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Citations for Roche :
8951 - 9000 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The heads were crushed on ice using a pestle in 0.5% NP-40 lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM EDTA, pH 7.5) supplemented with protease inhibitors (Roche). The resulting lysates were clarified by centrifugation and the supernatants were collected ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 1 µg of total RNA using the KAPA Stranded mRNA Hyperprep Kit (96rxn) (Roche, KK8581) according to manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 300 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min on ice) ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 50 µL cold nuclear lysis buffer (1% SDS, 10 mM EDTA, 50 mM tris-HCl pH8.0, 100U Ribolock inhibitor, Thermo, 1x protease/phosphatase inhibitor, Roche) and lysed (10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 5 ng amplified DNA and 1 μM forward and reverse primers and run on a LightCycler 480 machine (Roche) using the following protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Molecular Biology 2024Quote: Cells were harvested and protein was extracted using lysis buffer (20mM HEPES pH7.4, 2mM MgCl2, 40mM NaCl and 1% Triton-X) containing complete mini EDTA protease inhibitor (Roche) at 1 in 50 concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... cells were lysed with TNE-lysis buffer (50mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and cOmplete protease inhibitors, Roche) and prepared for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for western blots presented in this work were as follows: anti-HA monoclonal antibody (mAb) 3F10 (diluted 1:2,000) (Roche); mouse anti-GAPDH mAb (1:20,000).
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bioRxiv - Cancer Biology 2024Quote: ... or protein extraction (50 mM Tris-HCl, 1% NP40, 0.15 M NaCl, pH 8) supplemented with phosphatase (PhoStop, Roche) and protease inhibitors (Complete ...
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bioRxiv - Immunology 2023Quote: ... the hydrogels were carefully collected using cut 1000-µL pipette tips and incubated 15 minutes at 37°C in a 0.5 mg.mL-1 Collagenase (Roche, 11088866001) solution in DMEM/F12 ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were then ground to a fine powder in a mortar and pestle over liquid nitrogen and resuspended in freshly prepared extraction buffer (2mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% v/v NP40, 5% v/v glycerol, 1mM PMSF, 1ml Roche Protease Inhibitor cocktail per 30g input tissue ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with DPBS, resuspended in lysis buffer (1% NP40 in PBS, pH 7.4 with protease inhibitor cocktail (Roche), and 2.5% v/v RNase inhibitor) ...
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bioRxiv - Neuroscience 2024Quote: ... and blocked for 1 hour in blocking buffer consisting of 20% sheep serum and 2% blocking reagent (11096176001, Roche) in MABT ...
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bioRxiv - Biochemistry 2023Quote: ... after removal of the medium cells were lysed in 1 mL of lysis buffer (50 mM Hepes [pH 8.0], 150 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 % Glycerol, supplemented with 0.55 % Nonidet P40 substitute [Roche] and cOmpleteTM EDTA-free Protease Inhibitor Cocktail [Roche]) ...
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bioRxiv - Developmental Biology 2024Quote: ... heads were rinsed for 5 minutes in PBST five times and incubated in Blocking Reagent (Roche, 1:5000 dilution) for 60 minutes ...
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bioRxiv - Microbiology 2024Quote: ... gondii RH tachyzoites lysed in NP-40 lysis buffer (1% NP-40, 150 mM NaCl, 50 mM Tris [pH 8.0]) supplemented with complete protease inhibitor (Roche). The insoluble material was removed from the lysate by centrifugation at 10,000 x g for 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and resuspended in ice-cold lysis buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1 × cOmplete EDTA-free protease inhibitor (Roche), 1 mg/mL DNase and lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... resuspended in extraction buffer (SDS 2%, 50 mM Tris-Cl pH 7.4, 1 mM PMSF, 2x cOmplete protein inhibitor cocktail (Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were lysed in immunoprecipitation lysis buffer (50 mM Tris [pH = 7.5], 150 mM NaCl, 1% IGEPAL, 10% Glycerol) with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.1 % SDS, 150 mM NaCl, 10 mM EDTA, 20 mM Tris-HCl pH7.5 and complete protease inhibitor mix, Roche) was used to prepare protein lysates ...
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bioRxiv - Biochemistry 2024Quote: Homogenized lungs were 10-fold diluted starting 1:10 in infection media (DMEM containing 0.1% BSA (fraction V; Roche), 10 mM HEPES (Corning) ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 150 ml lysis buffer (50 mM Hepes pH 7.5, 50 mM NaCl, 1 mM MgCl2, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) at 4°C and loaded 3 times in a CellD disrupter (Constant Systems) ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl extraction buffer (Tris-HCl 50 mM pH 6.8, SDS 2%, DTT 2 mM and 1× protease inhibitors (Roche) and centrifuged for 5 min at 13,000g at 4OC ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), and washed again in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transfected with Tau P301L-V5 encoding full length human tau with P301L mutation and V5 tag (GKPIPNPLLGLDST) and TFEB-GFP at a 2:1 ratio of tau:TFEB (X-tremeGENE 9, Roche). 24 hours later the media was changed and 40 µL of Pff was added to the culture along with 200 nM Bafilomycin (Sigma ...
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bioRxiv - Bioengineering 2023Quote: Liver tissue was lysed using RIPA buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, cOmplete® Mini Protease Inhibitor Cocktail [Roche Diagnosis ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.6; 15 mM NaCl; 10% glycerol; 0.5% Tween 20; 10 mM imidazole; 1 mM DTT; 20 mM NaHCO3; Roche protease inhibitors cocktail without EDTA) ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were blocked using non-fat dry milk and stained with an anti-GFP primary (Roche; 1:1000) and a goat anti-mouse secondary antibody coupled to horseradish peroxidase (HRP ...
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bioRxiv - Synthetic Biology 2023Quote: ... was amplified in a 5ul qPCR reaction using x2 Lightcycler 480 SYBR Green 1 Master mix (Roche Diagnostics 04707516001) according to the manufacturer’s protocol (15sec at 95℃ and 30sec at 60℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... and frozen in 600 µL of ChIP lysis buffer plus with protease inhibitors and 1 mM PMSF (Roche 11836170001) [ChIP lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4°C with anti-digoxigenin-alkaline phosphatase antibodies (1:5000; Roche; 11093274910; AB_514497) diluted in blocking solution ...
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bioRxiv - Genomics 2023Quote: ... Cells were centrifuged at 500 x g for 5 min at 4C and 500 uL 1X PBS + 1% BSA + DAPI + 0.5U/uL Protector RNAse Inhibitor (Roche) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 500 mM NaCl, 0.4% [wt/vol] SDS, 5 mM EDTA, 1 mM DTT and 1x cOmplete Protease inhibitor cocktail, Roche) for 20 min on a rocker at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 1 ml SEH buffer (20 mM HEPES pH 7.4, 1 mM EDTA, 0.6 M Sorbitol, 1x cOmplete™ Tablets mini EDTA-free protease inhibitor [Roche]). Resuspended yeast cells were transferred into screw cap tubes containing 500 µl glass beads ...
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bioRxiv - Molecular Biology 2023Quote: Cell lysates were extracted using Igepal lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris pH 7.4) enriched with protease inhibitor cocktail (Roche). Proteins were analyzed by SDS-PAGE and immunoblotting.
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bioRxiv - Immunology 2023Quote: ... These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of Mosunetuzumab (Genentech, Roche): 0 ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were centrifuged at 1,000 x g for 1 min at RT prior to loading on a LightCycler 480 (Roche) using 3-step cycling ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RT-qPCR reaction volume was 1.5⍰μL and consisted of 1⍰μL of LightCycler 480 SYBR Green I Master Mix (Roche) containing 0.5⍰μM of PCR primer (Eurogenetec ...
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bioRxiv - Biochemistry 2023Quote: ... were homogenised with 1:4 weigth per volume diethylamine (DEA) buffer (50 mM NaCl, 0.2% diethylamine, pH 10, cOmplete Protease InhibitorsTM, Roche). After 60 min at 130000xg and 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Pellet was resuspended in high-salt buffer (750 mM NaCl, 10 mM Tris, pH 7.6, 1 mM EDTA, protease inhibitor tablet (Roche)) and lysed by sonication at 60% power using a probe sonicator (Branson sonifier 450 ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) or Lysis buffer (1 % SDS, 50 mM Tris pH6.8 and 10 % Glycerol) supplemented with protease inhibitor cocktail (Roche) and subsequently quantified using Pierce BCA Protein Assay Kit (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Tissues were washed in three changes of 0.2× SSC at 44°C for 15 minutes each and blocked in 4×SSC containing 1% blocking reagent (Roche) in 4× SSC for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... and lysed using NP-40 lysis buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% NP-40) supplemented with Complete Protease Inhibitor Cocktail (Roche) for 30 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed by sonication in 700 mM NaCl;20 mM HEPES 7.5 containing 1 protease inhibitor tablet (Roche) + 5ul Turbonuclease ...
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bioRxiv - Neuroscience 2023Quote: ... rat cortical neurons cultured as described above were lysed directly in buffer (50 mM HEPES pH 7.0, 2% SDS, 1 mM EDTA plus protease inhibitor mixture (PIC, Roche) and 20 mM MMTS (to block free thiols) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...