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Citations for Roche :
8751 - 8800 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... The lysis was performed in RIPA buffer (1% SDS, 10 mM Tris pH 8.0, containing protease and phosphatase inhibitor cocktail tablets (Roche)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Wing discs of L3 larvae were hybridized with probes overnight at 56 °C using standard procedures and visualized using anti-Dig-AP (1:1,000; Roche). Primers used for generating PCR templates are listed in Key Resources Table.
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in 1x lysis buffer (1% NP-40, 100mM Tris, pH 8.0, and 150mM NaCl) containing a protease inhibitor mixture (Roche) and shaken 60 mins at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Eye discs were then removed from the mouth hooks and blocked for 1 hour in 1X PBX:Western Blocking Reagent (Roche) at room temperature with nutation ...
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bioRxiv - Developmental Biology 2021Quote: ... apob-1 and apob-2 levels were detected using the Fast Start Essential Green DNA master mix (Roche 06924204001) on a Roche LightCycler 96 instrument ...
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bioRxiv - Immunology 2021Quote: ... were lysed using for one hour with ice-cold 1% NP-40 lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM EDTA) supplemented with complete mini (Roche) and HALT (Invitrogen ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HA-epitope tagged recombinant proteins were detected using a rat-derived monoclonal anti-HA antibody (dilution 1:200, Roche) followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200 ...
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bioRxiv - Developmental Biology 2020Quote: ... they were hybridized overnight at 65°C for three nights and incubated overnight with 1/2,000 alkaline-phosphatase conjugated with anti-DIG Fab fragment (Roche) at 4°C for three nights ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h and then incubated overnight at 4 °C in block solution with diluted DIG-antibodies (1:5,000) conjugated with alkaline phosphatase (AP) (Roche). To visualize WISH signal ...
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bioRxiv - Developmental Biology 2020Quote: ... 10 µl reaction mix was mixed with 290µl IP buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) containing protease inhibitor cocktail tablet (Roche) and 2 µg/ml of anti-FLAG (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... treatment for 1 hour at 37°C and cDNAs were amplified with KAPA HiFi Hotstart Ready Mix (Roche, KK2602). PCR reactions were conducted as follow ...
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bioRxiv - Developmental Biology 2021Quote: ... blocked in 1xBM Blocking/PBST at RT for 2 hours and incubated with anti-DIG-POD (1:1,000, 11207733910, Roche) at 4°C overnight ...
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bioRxiv - Genomics 2022Quote: ... 100µl of fly heads/∼107 S2 cells were homogenized in 500µl/1ml lysis buffer (50mM TRIS HCL 7.4, 150mM NaCl, 1mM EDTA, 1% TRITON X100 and protease inhibitor tablets [Roche]). Homogenate was kept on ice for 30 min and then sonicated using a Bioruptor sonication device (Diagenode ...
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bioRxiv - Microbiology 2022Quote: ... comprising 0.5 μL of DNA (40 ng/μL) and 1 μL of LightCycler 480 SYBR Green I Master mix (Roche) containing 0.5 μM PCR primers (Eurogenetec SA) ...
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bioRxiv - Molecular Biology 2021Quote: ... in a total volume of 40 µl to 2.4 pmole trypSL in the presence of 1 mM ATP and 20 U RNasin (Roche) in vaccinia capping buffer ...
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bioRxiv - Molecular Biology 2021Quote: In situ antibody stainings were done as described previously 31 using rat anti-HA (MAb 3F10, 1:20; Roche), rabbit anti-FLAG (M2 ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with an established dilution in fresh-prepared sample buffer (1% bovine serum albumin and 0.05% Tween-20 in PBS) supplemented with protease inhibitor (cOmplete, Roche). Detection antibody (R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... The pellet was resuspended in 5ml lysis buffer (20 mM Tris, 150 mM NaCl, 1% NP40 pH 7.5, plus a tablet of protease inhibitor cocktail from Roche) per mg of cell pellet and incubated at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... washed in cold PBS and incubated on ice in cold swelling buffer (10 mM Tris-HCl pH 8, 10 mM NaCl, 0.2% NP-40, 1 mM AEBSF and 1x complete mini EDTA-free proteasome inhibitor, Roche) for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: Mouse livers were homogenized with a motorized tissue grinder in STM buffer (Tris-HCl pH 7.4, 5 mM MgCl2, 250 mM Sucrose, 1 mM DTT, supplemented with 500 µM PMSF, Protease Inhibitor Cocktail [Roche, Basel ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed in 500 μl lysis buffer (50mMTris-HCl, pH8.0, 100mM NaCl, 10m MEDTA, 5% glycerine, 1% NP- 40 and protease inhibitor cocktail (Roche)) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 mM NaCl, 5 mM MgCl2, 0.5% NP-40, 1 mM DTT, and 1x cOmplete EDTA-free protease inhibitor cocktail [Roche]), washed once with PBS ...
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bioRxiv - Genomics 2020Quote: ... Between 60-80 mg pellets were lysed in 1mL lysis buffer (50mMTris-Cl pH 7.0, 10mM EDTA, 1%SDS, 1mM PMSF, complete protease inhibitor cocktail (Roche), 0.1U/mL RiboLock RNase inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were incubated with 50 μl of 1:10 Enzyme:TUNEL reagent (TdT and fluorescein-dUTP) (Roche, Cat No. 11684795910) at 37°C for 3 h in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... the cell pellets were resuspended in lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 % NP-40, 5 mM MgCl2, 5 % glycerol and protease inhibitor cocktail [Roche]) and incubated for 20 min at 4 °C with end-over-end rotation ...
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bioRxiv - Cell Biology 2019Quote: ... 100 mM KOAc pH 7.5, 10 mM MgSO4, 10 µM ZnSO4, 1 mM PMSF and complete protease inhibitors from Roche), and disrupted by bead beating with a FastPrep 24 (MP Biomedical ...
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bioRxiv - Microbiology 2019Quote: ... Immunoprecipitated proteins were immunoblotted for GFP- or HA-epitope tagged ubiquitin with antibodies for GFP (1:3,000, Roche #11814460001) or HA (1:4,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM ATP (+ 2 mM DTT for Strep-KaiC36803 proteins)] including protease inhibitors (protease inhibitor cocktail, Roche or Nacalai). Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences ...
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bioRxiv - Immunology 2019Quote: ... containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Immunoblots were carried out using the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were rinsed twice with PBS and resuspended in 1 mL lysis buffer (1X PBS, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS) supplemented with protease inhibitor cocktail (Roche, #11836170001). Chromatin was sheared to 200-500 base pairs using a Covaris E220 sonicator and cleared by centrifugation for 15 minutes at 19,000 × g ...
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bioRxiv - Physiology 2019Quote: ... Tissue was placed in ice-cold hypotonic lysis medium (HLM, 10 mM Tris, pH 7.4, 1 mM EDTA) in the presence of a protease inhibitor cocktail (Roche), minced and homogenized by 20 strokes in a glass homogenizer ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were lysed in lysis buffer (20 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 1 mM DTT and complete EDTA-free protease inhibitors cocktail [Roche]) supplemented with 0.02% NP40 and 1 mM PMSF ...
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bioRxiv - Developmental Biology 2019Quote: ... Trunks from ∼60 embryos were homogenised in 1ml solubilisation medium [2%w/v CHAPS in PBS made 1mM with sodium orthovanadate and 1 tablet cØmplete protease inhibitor cocktail (Roche) per 50ml of solution] on wet ice by shearing through a 20G then 26G needle ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then vigorously resuspended in 1ml of fresh ice-cold lysis buffer (50mM Tris pH 8, 150mM NaCl, 0.5% NP-40, 1 tablet of complete protease inhibitor (04693159001, Roche)) and transferred to 9 ml of pre-chilled lysis buffer and incubated for 20 min on ice ...
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bioRxiv - Immunology 2019Quote: ... diluted 1:150 in washing buffer (45 min) and 4’,6-diamidino-2-phenylindole counterstain (DAPI; Roche/Sigma-Aldrich) diluted 1:250 in TBS (15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Every 10 million cells were lysed by 300μL 1% SDS include freshly made Protease Inhibitor (PI) cocktail (Roche #4693132001), sonicated with Diagenode Bioruptor Pico and diluted with 2.7mL PBA (1x PBS + 0.5% BSA ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.15 μM each of PaaDlp-2_R1 and R2 in 1 × KAPA HiFi HotStart ReadyMix (KAPA Biosystems, Wilmington, WA, USA) and a 2-μL sample of eDNA ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... before lysing in lysis buffer ((1% Triton X100, 50 mM HEPES, 150 mM KCl, 1 mM MgCl2, pH 7.4, with 1x EDTA-free protease inhibitors, Roche) at 4 °C for 2 hrs ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: ... The samples were incubated on ice for 10min and then lysates were diluted 1:2 with CNBr IP wash buffer (50 mM Tris–HCl pH 7.5, 150mM NaCl, 1mM EDTA, 1/50 protease inhibitor tablets [Roche]). The homogenates were incubated on ice for 15min and were cleared by centrifugation at 12,000g for 15min at 4°C ...
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bioRxiv - Genetics 2021Quote: Neuronal cultures were lysed in 20 µl of NP40/SDS buffer (1% Nonidet P40/0.01% SDS lysis buffer and protease inhibitors (Roche) per well ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for one hour in donkey anti-mouse FITC antibody (1:200) in 0.3% Triton-X100 supplemented with 1X blocking buffer (Roche). After washing with PBS for 5 min ...
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bioRxiv - Immunology 2021Quote: ... Pellets of cross-linked cells were resuspended in shearing buffer (10 mM Tris-HCl pH 8, 0.1% SDS, 1 mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche), and chromatin was sheared to an average fragment size of 200-250 bp ...
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bioRxiv - Genetics 2020Quote: ... Cells pellets were combined and lysed in a denaturing urea buffer (8 M urea, 0.1 M ammonium bicarbonate, pH 8, 150 mM NaCl, 1 Roche mini protease inhibitor tablet without EDTA/10 ml ...
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bioRxiv - Immunology 2021Quote: ... coated with H-2Kb-OVA and ICAM-1 at a 1:1 ratio for various times at 37 ºC and immediately lysed in 2× cold lysis buffer containing phosphatase inhibitors (1 mM NaF and 0.1 mM Na3VO4) and protease inhibitors (cOmplete mini cocktail, EDTA-free, Roche). Activation of PI3K and MAP kinase signaling was assessed by immunoblot for pAkt (Phospho-Akt (Ser473 ...
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bioRxiv - Immunology 2021Quote: Single-cells were sorted into PCR tubes containing 1μl of cell lysis solution (1:10 Cell Lysis buffer(Roche), 10U/μl Rnasin plus Ribonuclease inhibitor (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Each tissue sample was dissociated using 2.4 mL of homogenization buffer (10 nM Tris pH 8, 5 mM MgCl2, 25 mM KCl, 250 mM sucrose, 1 μM DTT, 0.5x protease inhibitor [cOmplete, Roche #4693159001] ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to 8 cycles of lysis in a MagNALyser (Roche – 6000 rpm, 1 min on, 2 min off). Lysate was transferred to fresh microfuge tubes and clarified by centrifugation (13,000 rpm ...
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bioRxiv - Cancer Biology 2021Quote: GSDMB2-HA expression was analyzed by immunohistochemistry in 5µm-thick tissue sections using rat anti-HA (1:200; 3F10, ROCHE) or mouse monoclonal anti-GSDMB (1:10 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mM ATP and 10% glycerol) containing 1 mmol/L PMSF and DNase I (Roche Diagnostics Corp., IN, USA) to 5 µg/mL ...