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Citations for Roche :
9001 - 9050 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Cell pellets were snap frozen in Eppendorf tubes and lysed with glass beads in 500 μl GFP pull-down buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of OsHV-1 and total bacteria were performed using quantitative PCR (qPCR, Roche LightCycler 480 Real-Time thermocycler) with the following program ...
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bioRxiv - Cell Biology 2023Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche, 5401151001) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibodies were incubated for 1 h at 37°C while nuclei were counterstained with DAPI (Roche) at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... washed and resuspended in lysis buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM MgCl2; 10% Glycerol; Complete protease inhibitor (Roche); 1 mM PMSF or 2 mM AEBSF-hydrochloride (BioChemica APA1421.0001) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Genetics 2023Quote: Mouse tissue was homogenized in 400 μl of RIPA homogenizing buffer (50-mM Tris HCl pH 7.4, 150-nM NaCl, 1-mM EDTA, supplemented with protease-inhibitor cocktails (Roche)) and lysed with a MagNA Lyser ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hr and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... released by trypsin and incubated in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then released by trypsin and lysed in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti HA-HRP antibody (Roche 39 #12013819001; 1:2,000).
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed in with glass beads in 500 µl SPOT PD buffer (20mM HEPES pH 7.4, 150mM KOAc, 5% Glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were taken up in sonication buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, cOmplete protease inhibitor by Roche) at a ratio of 10 million cells in 150 µL (120 uL for ChIP-seq) ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Cell Biology 2023Quote: ... The clarified lysate was then diluted 3.75 times using dilution buffer (10 mM Tris–HCl, pH 7.4, 2 mM EDTA, 1 mM DTT, protease inhibitor cocktail tablet (cOmplete, Roche) and phosphatase inhibitor (phosSTOP ...
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed with TNE-lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP40, and complete Protease Inhibitors, Roche) for 30 min on ice then spun down at 11,000 rpm for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... 10 mM NaCl, 1% SDS, 0.1 mM dithiothreitol, 20 μM PMSF, 0.5% β-mercaptoethanol, 1X proteinase inhibitor from Roche) and centrifuged for 10 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Nonidet P-40) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10ml) (11836170001, Roche) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial pellets were harvested by centrifugation and resuspended in 50 mL of lysis buffer (20 mM Tris pH 7.5, 500 mM NaCl) to which 1 protease inhibitor tablet was added (Roche). Bacterial cells were lysed by sonication with 10 s pulses for 10 min and cell debris was removed by centrifugation at 25,000 x g for 40 min and cleared cell lysate was filtered through 0.45 μm filters ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed with 500 μl acid-washed glass beads in 500 μl GFP-Trap pulldown buffer (20 mM HEPES, pH 7.4, 150 mM KOAc, 5% glycerol, 1% GDN, Roche Complete Protease Inhibitor Cocktail EDTA free, Roche) using a FastPrep (MP biomedicals) ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... coli were harvested and disrupted by sonication in the lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP40) supplemented with protease inhibitor cocktail (cOmplete, Roche). After centrifugation at 40000 g for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Cell Biology 2023Quote: Cells were resuspended in cold homogenization buffer (250 mM sucrose, 250 mM HEPES/KOH pH 7.4, 1 mM EDTA) supplemented with cOmplete protease inhibitors (Roche) and lysed by Dounce homogenization on ice ...
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bioRxiv - Neuroscience 2023Quote: ... 7.5×105 of S2 cells were lysed in Lysis Buffer (50 mM Tris at pH 6.8, 150 mM NaCl, 1% NP40, protease inhibitor cocktail [Roche]), immunoprecipitated with 1 μg of GFP antibody (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... for 10 min and resuspended in lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were next rinsed 3x 15 minutes in room temperature TBST (Tris-buffered saline [pH 8.5] with 1% Tween 20) and blocked for two hours in 20% DIG buffer (Roche) in TBST.
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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Brief Change in Dopamine Activity during Consolidation Impairs Long-Term Memory via Sleep DisruptionbioRxiv - Neuroscience 2023Quote: ... primary antibodies used to detect GRASP signals was mouse monoclonal anti-GFP (1:200, Cat# 11814460001, Roche Applied Science). Samples were then washed in PBS-T at 4 ℃ for 10 min for 3 times ...
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bioRxiv - Biochemistry 2023Quote: ... under gravity flow, washed with PER2 buffer (1 x TBS pH 7.5, 1mM EDTA, 5% glycerol, EDTA-free protease inhibitors (Roche)) and eluted with 3x flag peptide in PER2 buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Microbiology 2023Quote: ... blocked in 5 % milk and probed with α-GFP (1:1,000 in 5 % milk; Roche Diagnostics GmbH, Mannheim, Germany), for GFP-based sensor probing ...
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bioRxiv - Genomics 2023Quote: ... The cell pellet was resuspended with ice-cold Galbraith’s buffer amended with bovine serum albumin (BSA, 0.05% w/v) and cOmplete protease inhibitor (1×, Roche 11697498001), lysed by repeated pipetting ...
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bioRxiv - Microbiology 2023Quote: ... The nuclear pellets were resuspended in nuclear lysis buffer (50mM Tris-HCl pH 8.0, 10mM EDTA, 1% SDS, 1mM PMSF, cOmplete Protease Inhibitor Cocktail (Roche)) and vortexed at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 million cells were suspended in 500 μl SDS lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, protease inhibitor (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in 750 μl Tris lysis buffer (50 mM Tris, 0.2% NP-40, 150 mM NaCl, 5 mM EDTA, 5.7 mM PMSF, and 1 x Roche cOmplete). For whole organ samples (either whole detunicated testis or thymus) ...
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were harvested by centrifugation and resuspended in 20 mL of lysis buffer (50 mM Tris pH 7.5, 300 mM NaCl, 2 mM MgCl2 and 1 mM DTT, 1X Roche EDTA-free Complete protease inhibitor cocktail) ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...