Labshake search
Citations for Roche :
9151 - 9200 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Cell toxicity at different concentrations of drugs as indicated was determine using the Cell Proliferation Reagent WST-1 (Roche Diagnostics) or cellular 96® Aqueous One Reagent viability reagent according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were then lysed using 1x RIPA buffer (Thermo) supplemented with 3x HALT Protease inhibitors (Thermo) and 1 × PhosSTOP (Roche), 2 μl (500 Units ...
-
bioRxiv - Cancer Biology 2020Quote: ... Transposed chromatin was amplified using customized Nextera PCR Primer 1 and Primer 2 (barcode) (31) and HotStart KAPA ReadyMix (Roche). Libraries were quantified by Qubit using the DS DNA HS kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... for 1h at RT and incubated with a primary antibody diluted in blocking solution (sheep anti-digoxigenin, 1/200, Roche) overnight at +4°C in a dark humidified chamber ...
-
bioRxiv - Cell Biology 2021Quote: ... pH 7.4, 200 mM NaCl, 1% NP-40, 2 mM MgCl2, 10% glycerol, NaF +Na3VO4, complete mini tablets without EDTA, Roche). Lysates were incubated on ice for 15 minutes and centrifuged at 17,000xg at 4°C for 10 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... The gel piece was vacuum dried and treated with sequencing grade trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche) at 37°C for 10-16 hours ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1·104 cells per well were seeded onto E-Plates 16 and analysed in the xCELLigence RTCA DP system (Roche) for 48 h in quadruplicates.
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 mM EDTA) supplemented with a cocktail of protease inhibitors (Complete Protease Inhibitor without EDTA, Roche Applied Science, Indianapolis, IN) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 3 ...
-
bioRxiv - Biophysics 2022Quote: ... the force was kept at 11 pN and Pif1 was added to the microfluidic chamber at a concentration of 6 nM and 1 mM ATP Lithium salt (Roche) in G4-Pif1 buffer ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were resuspended in fractionation buffer (20 mM HEPES-KOH pH 7.6, 1 mM EDTA, 1x cOmplete protease inhibitor (Roche 11873580001)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2ml of lysis buffer (50mM Tris pH 8.0, 150mM NaCl, 5mM MgCl2, 1%Triton X100, 1X Protease inhibitor (Roche,11836170001), 10% glycerol ...
-
bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with 1:4,000 diluted peroxidase-conjugated anti-DIG sheep antibody (11-207-733-910; Roche Diagnostics). Subsequently ...
-
bioRxiv - Neuroscience 2022Quote: ... cells or brain tissue were lysed in mild lysis buffer (50mM Tris-HCl, 150mM NaCl and 1% NP-40) with cOmplete mini protease inhibitor cocktail (Roche) and centrifuged at 12 000g for 10 minutes at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... cells were recovered and lysed in 500 µl hypotonic lysis buffer (10 mM Tris-HCl pH 8, 10 mM KCl, 1× complete protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Developmental Biology 2022Quote: ... TUNEL staining was performed using ApopTag Red In Situ Apoptosis Detection Kit (S7100, MerckMillipore) combined with peroxidase (POD) coupled anti-DIG antibody (1/200, #11207733910, Roche) followed by tyramide-based amplification61.
-
bioRxiv - Cell Biology 2022Quote: ... collected and lysed with NP-40 extract buffer (50 mM Tris, pH 8, 150 mM NaCl, 1 % NP-40) containing protease inhibitors (#11873580001 Roche) and 30 µg of lysate was migrated in 10 % SDS-PAGE gels (Bio-Rad Laboratories) ...
-
bioRxiv - Biochemistry 2022Quote: ... HEK293T cells were lysed in 100-200 μL of TRPA1 lysis buffer (20 mM HEPES, 150 mM NaCl, 1 mM IP6, 20 mM DDM, 1x EDTA-free Roche Complete Protease Inhibitor Cocktail ...
-
bioRxiv - Cancer Biology 2022Quote: Cells were resuspended in lysis buffer (20 mM Tris pH8, 50 mM NaCl, 1% NP40, cOmplete EDTA-free Protease Inhibitor Cocktail (Roche)) or in MIB (to analyze mitochondria) ...
-
bioRxiv - Microbiology 2022Quote: ... and cells were lysed in assay buffer (1% NP-40) supplemented with cOmplete™ mini EDTA-free protease inhibitor (Roche) and PhosSTOP phosphatase inhibitor (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... Blotting was done as described in (Konrad et al. 2010) using primary rat-derived anti-HA antibody (dilution 1:500, Roche) followed by anti-rat (dilution 1:2000 ...
-
bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche). 1:5 diluted cDNAs was used for qPCR by using KAPA SYBR® FAST qPCR Master Mix (5X ...
-
bioRxiv - Cell Biology 2022Quote: ... then precipitated and cleaned before resuspension in hybridization buffer (65% formamide, 5X SSC, 5X Denhardts with 1% blocking reagent (Roche), 0.5 mg/mL salmon sperm DNA added fresh) ...
-
bioRxiv - Neuroscience 2020Quote: ... the sections were incubated overnight at +4 °C with the antibody anti-digoxigenin-AP Fab fragment (#11093274910, Roche, 1:1000) diluted in blocking solution in a humidity chamber ...
-
bioRxiv - Microbiology 2020Quote: ... proteins were collected by lysing in lysis buffer (50mM TRIS-HCl, 150mM NaCl, 1% NP40 and protease inhibitor cocktail [Complete Mini EDTA-free, Roche]) and subjected to western blotting with the corresponding antibody (anti-GFP ...
-
bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at 4°C with primary antibody for rat anti-HA (1:200; 12013819001, RRID:AB_390917, Roche, Basel, Switzerland), rabbit anti-GFP (1:200 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and incubated with α-DIG or α-fluorescein antibody conjugated with alkaline phosphatase (Roche, 1:2000 in 10% FBS/PBST) at 4°C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... pelleted and resuspended in 150 μl sonication buffer [(50 mM Tris pH 8, 1% SDS, 10 mM EDTA, 1x protease inhibitor (Roche)] ...
-
bioRxiv - Molecular Biology 2021Quote: ... un-probed input RNA was also subjected to Sanger sequencing: RNA was dissolved in 5 μl water and supplemented with 1 μl of 10 mM corresponding ddNTP (Roche), then reverse-transcribed into cDNA and analysed by PAGE as described below.
-
bioRxiv - Microbiology 2020Quote: ... 30 mM imidazole, 1mM DTT, 0.75% CHAPS, 1 μM DNases, 5◻mM MgCl2 and EDTA-free protease inhibitor cocktail [Roche]). Purification was similar as the above instead of the elution buffer used (50 mM HEPES ...
-
bioRxiv - Microbiology 2020Quote: Cells were washed once with ice-cold PBS and lysates harvested at the indicated times post infection with lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – complete mini EDTA-free protease inhibitor ...
-
bioRxiv - Neuroscience 2020Quote: ... slides were incubated overnight at room temperature with anti-DIG antibody conjugated with the alkaline phosphatase (1/5,000, Roche Diagnostics) in B1 containing 1% NGS ...
-
bioRxiv - Cell Biology 2020Quote: ... protein extracts were prepared as previously described (Alao et al., 2014) with addition of 1 × PhosStop phosphatase inhibitor cocktail (Roche Diagnostics Scandinavia AB ...
-
bioRxiv - Microbiology 2020Quote: ... 1 mM EDTA) supplemented with 150 mM NaCl and the Completeä Mini EDTA-free protease inhibitor cocktail (Roche Applied Bioscience). Cell aliquots (200 ml ...
-
bioRxiv - Immunology 2020Quote: ... 1 mM of EDTA) supplemented with protease and phosphatase inhibitors (EDTA-free protease mixture inhibitor; Roche Diagnostics, Almere, the Netherlands). The protein pellets were homogenized passing through a 27G needle ...
-
bioRxiv - Microbiology 2021Quote: ... Membranes were blocked in TBST with 5% milk protein and probed with the following antibodies: 1:1000 anti-HA (Roche), then 1:1500 goat anti-rat IgG-HRP (Dako) ...
-
bioRxiv - Neuroscience 2021Quote: ... there is a need to stop it after 2 hours by a single injection of diazepam (Valium®, 10 mg×kg−1, i.p.; Roche). Rats were then hydrated with 2 mL of saline solution (0.9% NaCl ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNAseq libraries were prepared from 1 μg of total RNA per sample using the KAPA Stranded mRNA Sequencing Kit (Roche). In short ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
-
bioRxiv - Neuroscience 2022Quote: ... Hippocampi were isolated and stored in 10 volumes of sucrose buffer 1 (320mM sucrose, 1mM NaHCO3, 1mM MgCl2, 0.5mM CaCl2, 1µM PMSF) containing protease inhibitor cocktail (Roche, Mannheim, Germany). Tissues were homogenized for 10 strokes using a motor-driven 2 ml Potter-Elvehjem homogenizer fitted with a Teflon pestle ...
-
bioRxiv - Genetics 2022Quote: ... Samples were blocked for 30 minutes in a solution of 1% BSA in 2x SSC with 0.1% tween at room temperature and then rhodamine-conjugated anti-digoxigenin (Roche, #11207750910) or FITC-conjugated anti-biotin (AbCam ...
-
bioRxiv - Cell Biology 2022Quote: ... 25mM Tris-HCl pH=7.5, 1% Triton-X, 10mM Na3VO4, 40mM β-glycerophosphate, 10mM NaF and protease cocktail inhibitor from Roche). 1mg of total protein lysate was incubated with 1µg/mL of purified GST or GST-SidI overnight at 4°C in a rotary mixer ...
-
bioRxiv - Cell Biology 2022Quote: ... Each DNA sample was diluted to 1 ng/μL and 2 μL were used for amplification using PrimeTime Gene expression Master Mix (IDT) with LightCycler96 (Roche) instrument ...
-
bioRxiv - Immunology 2022Quote: ... and spleen were harvested and homogenated in PBS for CFU counting or in isotonic buffer (Tris HCl 50 nM, EDTA 2 mM, PMSF 1 mM [Roche Diagnostics GmbH] ...
-
bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
-
bioRxiv - Cell Biology 2022Quote: 2 μL of FLAG or IgG pull down and of a 1:10 dilution of the INPUT were used for amplification using qPCRBIO SyGreen Mix (PCR Biosystem) in a LightCycler96 (Roche) instrument ...
-
bioRxiv - Cancer Biology 2019Quote: Reverse transcription (RT) reactions were carried out using 1 μg of RNA using Transcriptor RT enzyme (Catalog No. 03531287001) from Roche Diagnostics ...
-
bioRxiv - Neuroscience 2019Quote: ... Remaining pellets were washed with PBS and resuspended in 1% sarkosyl in PBS containing complete mini protease inhibitor cocktail (Roche), vortexed for 30 sec and incubated at room temperature for 1 hr ...
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclei were pelleted by centrifugation and resuspended in sonication buffer (50 mM Tris-HCl pH 8, 1% SDS, 10 mM EDTA, 1x cOmplete ULTRA Tablet (Roche)) and sonicated using a Bioruptor sonicator (Diagenode ...
-
bioRxiv - Neuroscience 2019Quote: ... and then incubated overnight at 4°C with sheep anti-DIG alkaline phosphatase conjugated antibody (1:2,000; Roche Applied Sciences). Sections were washed thrice with PTW buffer and twice with coloration buffer (100 mM Tris-HCl ...
-
bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 hrs at 37°C in the dark with the in situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...