Labshake search
Citations for Roche :
9101 - 9150 of 9754 citations for Monoisodecyl Phthalate 100 Ug Ml In Mtbe Unlabeled Mono 3 7 Dimethyl 1 Octyl Phthalate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... 0.5 mM tris(2-carboxyethyl)phosphine (TCEP) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and EDTA-free protease inhibitors (Roche). The lysate was clarified by centrifugation and the proteins in the supernatant were purified by gravity Ni-nitrilotriacetic acid (Ni-NTA ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Sections were blocked for 1 h with 2% blocking reagent in MABT (Perkin-Elmer) and then incubated with anti-digoxigenin-POD (Roche), diluted 1:500 ...
-
bioRxiv - Genomics 2020Quote: ... cells were resuspended in 500 μl lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.3% NP40, 1× Roche Complete) and kept on ice ...
-
bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
-
bioRxiv - Genomics 2020Quote: ... Real-time quantitative PCR reactions were set up in triplicate with 2 μL of cDNA (1:4 dilution) per reaction using the FastStart Universal SYBR Green Master mix (Rox) (Roche) and run on a 7500 Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2019Quote: ... and incubated 2 hr at room temperature with a 1:5000 dilution of alkaline phosphatase-conjugated anti-DIG primary antibody in block solution (Roche, cat #11-082-736-103 ...
-
bioRxiv - Cell Biology 2019Quote: ... were lysed in NP-40 lysis buffer (50mM TRIS, pH7.5, 120mM NaCl, 1% NP-40) supplemented with Complete® protease inhibitor (Roche) and phosphatase inhibitor cocktail (Roche) ...
-
bioRxiv - Biochemistry 2020Quote: Cells were resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Triton X100, 0.3 mM TCEP, 1X Roche cOmplete tablet) and lysed by three cycles of rapid freeze-thaw ...
-
bioRxiv - Neuroscience 2019Quote: ... After 48 h of transfection cells were scraped and lysed in lysis buffer (20mM HEPES, 150mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton-X100, cOmplete Protease Inhibitor Cocktail (Roche), Benzonase (EMD Millipore) ...
-
bioRxiv - Physiology 2019Quote: ... Tissue slices were incubated with hybridization probes for 18 h at 55°C and then incubated with sheep anti-DIG and anti-FITC antibodies conjugated with peroxidase (POD; 1:250; Roche) for probe labeling using the InSituPro VSI robot (Intavis ...
-
bioRxiv - Molecular Biology 2019Quote: Total proteins were extracted in RIPA buffer containing 1 mM sodium orthovanadate and Complete Protease Inhibitor Cocktail (Roche Applied Science) and quantified using the Pierce™ BCA protein assay kit (Thermo Fisher) ...
-
bioRxiv - Developmental Biology 2020Quote: ... a final probe concentration of 0.1 ng/μL was used and the probe was detected using an alkaline phosphatase conjugated anti-digoxigenin antibody (1:1500; Roche) and NBT/BCIP (Roche ...
-
bioRxiv - Cancer Biology 2019Quote: Whole cell lysates from 5.0 × 106 cells were prepared using SDS-based buffer (5 mM EDTA, 1% SDS) supplemented with Protease inhibitor cocktail (Roche) and PhosSTOP EASY (Roche) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested after 48-72 h and resuspended in lysis buffer (20 mM HEPES pH 7.5, 300 mM NaCl, 10% glycerol, 30 mM imidazole, 1 mM TCEP and complete EDTA-free protease inhibitors cocktail [Roche]) and lysed by sonication ...
-
bioRxiv - Cell Biology 2019Quote: ... allowed to adhere overnight and transfected with the indicated splicing reporter constructs (1 µg/well) using X-tremeGENE 9 (Roche). As shown in the Key Resources Table ...
-
bioRxiv - Biochemistry 2019Quote: ... The cells were then pelleted again and resuspended in 150 μL of Lysozyme buffer (150 mM NaCl, 30 mM Tris–HCl pH 8, 10 mM EDTA, 1 mM TCEP, cOmplete protease inhibitor (Roche), 1 mg/ml lysozyme ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were harvested 48h after transfection and lysed in lysis buffer (50mM Tris-Cl pH 8, 150mM NaCl, 1% Triton, 1x Roche Complete Mini Protease Inhibitor Cocktail ...
-
bioRxiv - Neuroscience 2019Quote: ... using 30:1 molar ratio of isotope [35S] dATP (1250Ci/mmol, NEN) to oligonoucleotide and terminal deoxynucleotidyl transferase (TDT, Roche). Non incorporated nucleotide was removed by Biospin 6 chromatography columns (Bio-rad) ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: Antiviral azaBINOL B#24 was assessed for inhibition of HIV-1 reverse transcriptase using a commercially available colorimetric reverse transcriptase assay kit (Roche) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... 200 mM NaCl; 1mM EDTA, pH 8.0; 0.5 % Nonidet P-40; 1 tablet complete protease inhibitor per 50ml, Roche, Mannheim, Germany). For each pulldown ...
-
bioRxiv - Biochemistry 2021Quote: ... 150 mM sodium chloride (NaCl), 20% glycerol, and 1 mM Dithiothreitol (DTT, Scientific) supplemented with one Complete EDTA free protease inhibitor tablet (Roche) per 50 ml lysis buffer ...
-
bioRxiv - Bioengineering 2021Quote: ... S-phase Synchronous HeLa S3 cells were washed with ice-cold 1× PBS and lysed in a swelling buffer (20 mM HEPES, pH 7.5, 2 mM MgCl2, 5 mM KCl, 1 mM Dithiothreitol [DTT], and protease inhibitor cocktail [Roche; #11836170001]) supplemented with energy-regenerating mixture ...
-
bioRxiv - Genetics 2019Quote: ... The cell pellet was resuspended in cold sonication buffer (50mM Tris-HCl pH8, 10mM EDTA, 1% SDS) supplemented with protease inhibitors (Complete, Roche), and chromatin was sheared to an average size of 200-500bp by sonication for 35 cycles (30s ON/30s OFF ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated with 0.5% blocking reagent in PBT before an overnight incubation in 1:2000 anti-dig AP antibody (Roche, 11093274910) in 0.5% blocking reagent (Roche ...
-
Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
-
bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
-
bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
-
bioRxiv - Neuroscience 2020Quote: ... the samples were incubated with anti-digoxigenin conjugated with alkaline phosphatase (1:2500; sheep anti-DIG-AP Fab fragments, Roche Diagnostics ...
-
bioRxiv - Neuroscience 2020Quote: ... followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750; Roche). The colometric reaction was performed using mixture of 5-Bromo-4-chloro-3-indolyl phosphate (Roche ...
-
bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... 5% (v/v) glycerol and 1 mM Tris(2-carboxy-ethyl) phosphine (TCEP) containing an EDTA-free protease inhibitor tablet (Roche). The cell suspension was sonicated on ice and clarified by centrifugation at 27,000g for 15 min ...
-
bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
-
bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) analyses of cDNA (diluted at 1/10) were performed in triplicate with a LightCycler 480 II (Roche) using the SYBR Green I Master mix (Roche) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC) and liver (human hepatocellular carcinoma, HepG2) cells were assessed using a standard WST-1 (Roche) cell viability assay ...
-
bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Neuroscience 2021Quote: ... in DMEM medium supplemented with Glutamax and 10 % FBS were transfected using a 2:1 ratio X-treme GENE HP DNA transfection reagent (Roche) per μg of plasmid DNA with a total of 0.5 μg DNA per well ...
-
bioRxiv - Cancer Biology 2021Quote: ... A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche). Sequences of the primers used are listed in the reagents or resource table.
-
bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
-
bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended in ice-cold NP-40 Buffer (150mM NaCl, 1% NP-40, 50mM Tris pH8) containing EDTA free protease inhibitors (complete cocktail, Roche) and lysed using a 1ml tissue homogenizer (Fisher) ...
-
bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
-
bioRxiv - Biochemistry 2021Quote: Cells were washed 2x with PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1X PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
-
bioRxiv - Neuroscience 2021Quote: ... embryonic cortices were isolated and stored either in lysis buffer (48% Urea, 14 mM Tris pH 7,5, 8,7% Glycerol, 1% SDS, protease inhibitor cocktail (Roche, Basel, CH)) or RNAlater (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... 150 mM KOAc, 2 mM MgCl2, 1 mM CaCl2, 0.2 M sorbitol, 10mM PMSF, 2x protease inhibitor cocktails [Roche, 11873580001]), dropped into liquid nitrogen ...
-
bioRxiv - Plant Biology 2020Quote: ... cDNA was generated from 1 μg total RNA with qScript cDNA SuperMix (Quantabio) and analyzed on a LightCycler 480 II apparatus (Roche) with the SYBR Green I Master mix (Roche ...
-
bioRxiv - Microbiology 2020Quote: ... The plasmid NL4.3 and various mutants were transfected into JLTRG-R5 cells with 1 μg of viral DNA using Xtreme-GENE HP (Roche) Transfection reagent ...
-
bioRxiv - Plant Biology 2020Quote: ... and proteins were extracted in protein extraction buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 % glycerol, 2 mM EDTA, 5 mM DTT, 1 × EDTA-free Complete Protease Inhibitor Cocktail [Roche, USA] ...
-
bioRxiv - Genomics 2021Quote: ... Cells from two biological replicates were lysed with ChIP lysis buffer (5 mM PIPES pH8, 85 mM KCl, 1% Igepal) with a protease inhibitor (PI) cocktail (Roche). Nuclei were collected by centrifugation at 2,000 rpm ...
-
bioRxiv - Neuroscience 2020Quote: ... Lysis was performed using mechanical scraping in lysis buffer (1% IGEPAL-CA630 and 1mMPMSF with Roche complete protease inhibitor tablet) and subsequently spun down at 500xg for 5 minutes at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... The construct was used to transform wild type plants following the standard floral dip method (Clough and Bent, 1998) and selected with 50 mg L-1 Hygromycin (Roche). A total of 60 T1 plants were individually genotyped by PCR and sequencing ...
-
bioRxiv - Microbiology 2021Quote: Cells were washed once with ice-cold PBS and lysates harvested at indicated times post infection with lysis buffer (1% NP40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris HCl) supplemented with protease inhibitors (Roche – cOmplete mini EDTA-free protease inhibitor ...