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Citations for Roche :
6901 - 6950 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... incubation buffer containing a peroxidase-labeled anti-DNA mAb (Cell Death Detection ELISA, 11544675001, Roche Life Sciences, dilution 1:10). The plate was incubated for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples used in FISH analyses were incubated in anti-DIG-POD antibody solution (1:2000 dilution; Roche Diagnostics, Mannheim, Germany) rocking overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... The primary antibodies used in this study were an anti-HA rat monoclonal antibody (1:500 dilution, clone 3F10, Roche) and an anti-heat shock protein 70 (HSP70 ...
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bioRxiv - Microbiology 2024Quote: Duplicate aliquots of 1 mL were removed from each flask for subsequent RNA extraction with the High Pure RNA Isolation Kit (Roche). Extraction was conducted following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100, supplemented with protease inhibitor cocktail (cat. #A32965, Pierce) and phosSTOP Phosphatase Inhibitor Cocktail (cat. #4906837001, Roche)) ...
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bioRxiv - Immunology 2024Quote: The ear skin was minced finely with scissors and digested in RPMI1640 medium containing 333-666 µg/ml Liberase TL (1 vial with 5–10 mg pack size; Roche), 2 kU/ml DNase I (Merk Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were blocked and then incubated with 1/4000 concentration of anti-DIG Fab fragment conjugated with alkaline phosphatase (Roche) overnight at 4ºC ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Plant Biology 2024Quote: Seed histological sections were washed with Phosphate Buffered Saline solution (PBS) and digested with 1 mg/ml proteinase-K (Hoffmann-La Roche). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed 3x 15 minutes in room temperature TBST (Tris-buffered saline with 1% Tween 20) and blocked at room temperature for one hour in 10% DIG buffer (Roche) in TBST.
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bioRxiv - Immunology 2024Quote: ... Quantitative RT-PCR analysis was performed with selective primers (Supplementary Table 1) using the Light Cycler 480 SYBR Green I Master X2 Kit (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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bioRxiv - Immunology 2024Quote: ... Probed membranes were washed and incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit Abs for 1 hour and developed with Lumi-LightPLUS Western Blotting Substrate (Roche). Quantification was performed on Lumi-Light autoradiography films using ImageJ software.
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bioRxiv - Immunology 2024Quote: Cell lysates were prepared by lysing cells for 1 h at 4 °C in RIPA buffer supplemented with cOmplete protease inhibitor cocktail (Roche). The cell lysate concentrations were quantified using a BCA protein assay kit (ThermoFisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total proteins from T-ALL#1 cells were extracted with a RIPA buffer containing a cocktail of 1Xprotease inhibitors (11836145001, Roche) and 1X phosphatase inhibitors (P2850 ...
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Lobes were separated into clusters of 1-5 oocytes and the follicular layer was removed i) enzymatically with Liberase (Roche) and ii ...
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bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
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bioRxiv - Bioengineering 2024Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
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bioRxiv - Neuroscience 2024Quote: 1 µg of each total RNA sample was used to synthetize cDNA with Transcriptor First Strand cDNA synthesis Kit (Roche) according to the manufacturer’s instructions with the additional denaturation step at 65°C for 10 min ...
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bioRxiv - Neuroscience 2024Quote: Cells were harvested using a lysis buffer (TBS with 1% Triton X-100, 1x Roche EDTA free cOmplete protease inhibitor, 1x Roche pSTOP phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2024Quote: ... desoxycholic acid-Na-salt 1%, SDS 0.1%) (Serva electrophoresis, Germany) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Switzerland). Western blots were performed following routine protocols67 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of the total protein extract was incubated with 20 μL of Anti-HA Affinity Matrix (rat IgG1, Roche) or Anti-FLAG M2 Affinity Gel (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed in a NP-40 buffer (50 mM Tris, 150 mM NaCl, 1% Nonidet P-40, and Complete Mini protease inhibitor cocktail (Roche), Flag-tagged M117 protein was precipitated with anti-Flag and protein G sepharose ...
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bioRxiv - Systems Biology 2024Quote: ... Each cell pellet was resuspended in roughly 0.9 ml of lysis buffer consisting of 1% SDS and 200 mM EPPS with protease inhibitors (Roche 11836170001). The cell mixture was boiled at 95°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... solution then stained for 12 - 18 hours at 4°C in buffer containing 10% HISS and alkaline phosphatase-conjugated sheep anti-digoxigenin antibody (1:2000, Roche).
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bioRxiv - Biochemistry 2024Quote: ... and 0.5% sodium deoxycholate,100 µL buffer and ∼20 µL beads per 10 mg tissue) with 1 x PhosSTOP phosphatase inhibitor cocktail (Roche). ∼50 µg protein from each sample was then digested in two steps by Lys-C and trypsin ...
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bioRxiv - Molecular Biology 2024Quote: ... FISH probes were made by labelling 1 μg of DNA with BIO-nick translation kit or DIG-nick translation kit (Roche). Following addition of hybridization mix slides were incubated in a thermocycler with the following program ...
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bioRxiv - Genomics 2024Quote: ... Probes were added together (each at a 1:50 dilution) to hybridization buffer (70% deionized formamide, 10 mM Tris-HCl pH 7.5, 0.5% 10x Roche blocking solution). Diluted probes were denatured at 90°C and coverslips were denatured at 85°C ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in with TritonX-100 buffer (1% Triton X-100, 0.5 M EDTA, PBS, ddH2O) with protease/phosphatase inhibitors (cOmplete™ Protease Inhibitor Cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). The qPCR was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Systems Biology 2024Quote: ... then primary antibodies or lectins added at the following dilutions in IF blocking buffer: rat anti-HA 1:1000 (Roche), biotinylated GNL 1:1000 (Vector Labs) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... beads were washed between 10-15 times with wash buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). and ...
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bioRxiv - Neuroscience 2024Quote: ... mouse brains were homogenized at a 1:10 (w/vol) ratio of the brain in 1X TBS supplemented with protease inhibitor cocktail (Roche), using a Bead Beater (1 min ...
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bioRxiv - Bioengineering 2024Quote: ... 8-16 hCOs were lysed in 300 μl of 1% SDS in Milli-Q water with cOmplete ULTRA Protease Inhibitor Cocktail (Roche). Samples were vortexed ...
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bioRxiv - Developmental Biology 2024Quote: ... NvAxin expression was detected using NBT/BCIP as a substrate for the alkaline phosphatase conjugated to anti-digoxigenin fab fragments (1:5000; Roche).
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bioRxiv - Biophysics 2024Quote: ... The cells were resuspended in lysis buffer (1× PBS, 250 mM KCl, 2.5mM MgCl2, 1mM DTT, cOmplete EDTA free protease inhibitor cocktail; Roche, Benzonase), lysed ...
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bioRxiv - Bioengineering 2024Quote: ... sulfanilamide (Reagent I) and N-(1-Naphthyl)-Ethylenediamine in hydrochloric acid (Reagent II) from the “Nitrite/Nitrate Colorimetric Test” (Roche) kit (#11746081001 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then washed in 1 mL of PBS twice and lysed in ice-cold 1x RIPA buffer supplemented with protease inhibitor (Roche). Cells were lysed while shaking on ice for 20 min before transfer and tip sonication (3 x 1 s pulses at 30% amplitude) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein was eluted of beads with 10 mM EDTA and 1% SDS and the DNA was liberated from protein using Proteinase K (Roche). The DNA was purified using phenol chloroform extraction then treated with RNAse A ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed through regular hybridization buffer/SSC series and incubated in 1:1000 pre-absorbed anti-DIG-AP fab fragment (Roche)/5% sheep serum/1% blocking reagent (Roche)/1% DMSO/TNT at 4°C overnight ...
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bioRxiv - Evolutionary Biology 2024Quote: ... This was followed by a 1-hour incubation at 37°C with 10 µL of RNAse A (20mg/ml) (Roche). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% replacement NP-40, 10 mM EDTA, 25 mM HEPES, 1 mM TCEP, 10 μM Combretastatin, 10 μM Cytochalasin D, Roche Protease Inhibitor Mini EDTA free ...
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bioRxiv - Developmental Biology 2024Quote: ... Chromogenic asexual WISH samples were incubated with anti-DIG-AP (1:2000) and then developed with an NBT-BCIP solution (Roche) as previously described (King ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 10% heat inactivated sheep serum (HISS) and incubated at 4°C with anti-DIG antibody (1:2000, Roche) in TBST with 1% HISS overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... the optimal PCR cycle number was determined with an aliquot of 1 μL unpurified cDNA in a 10 μL qPCR containing 1X Kapa HiFi Hotstart Readymix (Roche), 1X SybrGreen and 0.2 μM UP primer ...
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bioRxiv - Developmental Biology 2024Quote: ... for 3 h at room temperature and then blocked in blocking solution (Anti-Digoxigenin-alkaline phosphatase-Fab fragments in incubation mix) (1:10000; Roche) overnight at 4°C with gentle shaking ...