Labshake search
Citations for Roche :
6751 - 6800 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... Thawed pellets were lysed in LB1 (50 mM HEPES-KOH pH 7.6, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.5% NP- 40, 0.25% Triton X-100, Roche protease inhibitor cocktail) for 20 min with rotation at 4°C and cleared by centrifugation at 300 x g for 5 min at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... The total contents of Tie1-HA and Tie1KD-HA were assayed in a parallel run using anti-HA antibody (1:2000, Roche). As a loading control ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were counted and directly transferred into CUT&Tag Wash buffer supplemented with 0.01% Digitonin (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1× Roche Protease inhibitor cocktail). Per experiment ...
-
bioRxiv - Immunology 2023Quote: ... tumor tissue was harvested digested with 1 mg/ml collagenase type I and 100 μg/ml DNase I (both Roche) and T cell infiltration analyzed by flow cytometry ...
-
bioRxiv - Neuroscience 2023Quote: ... Five volumes of 50 mM Tris pH 8 was added and proteins were digested over night with trypsin (1:50) (Roche) at 37°C while shaking at 800 rpm ...
-
bioRxiv - Molecular Biology 2023Quote: ... The cells were blocked and permeabilized overnight (STORM) or for 2h (STED and LSCM) (1% Roche Western Blotting Reagent (WBR), Merck ...
-
bioRxiv - Neuroscience 2023Quote: ... microvessels obtained in the pellet were combined into a new conical sterile tube and further digested with collagenase-dispase (1 mg/ml, Roche) and DNase I (6.7 μg/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... were lysed by sonication in ∼140 mL lysis buffer (50 mM Tris-HCl pH 8, 250 mM KCl, 1 mM TCEP) supplemented with protease inhibitor cocktail (Roche) and DNase I (5 μg/ml ...
-
bioRxiv - Microbiology 2023Quote: ... ChIP-seq libraries were prepared by Duke University Sequencing and Genomic Technologies Core Facility (Duke Sequencing Facility) from 1 ng of ChIP DNA using the KAPA HyperPrep Library Kit (Roche) to generate Illumina-compatible libraries ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC), and liver (human hepatocellular carcinoma, HepG2) [45–50] cells were assessed using a standard WST-1 (Roche) cell viability assay ...
-
bioRxiv - Cell Biology 2023Quote: 293T cells to express FLAG-tagged human RSPH proteins transiently were lysed with 1% Triton X-100 in 1X HEPES buffer with EDTA-free protease inhibitor cocktail (Roche) by rocking at 4 °C for 2 hours ...
-
bioRxiv - Cell Biology 2023Quote: Cells were washed 2x with 1x PBS to remove excess biotin and lysed in highly stringent washing buffer 5 (WB5; 8 M urea, 1% SDS in 1x PBS) supplemented with 1x protease inhibitor cocktail (Roche) and 50 μM NEM ...
-
bioRxiv - Cell Biology 2023Quote: Total cell lysates were generated from E10.5 embryos using CHAPS buffer (10 mM HEPES, pH 7.4, 150 mM NaCl, 1% CHAPS plus complete protease inhibitor cocktail (Roche, #11697498001)) ...
-
bioRxiv - Immunology 2023Quote: Spleens were injected with HBSS (with Ca2+ and Mg2+) containing 1 mg/mL of collagenase D and 10 μg/mL of DNase I (Roche) and incubated for 20 minutes at 37°C ...
-
ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Phosphorylated and annealed oligos were diluted 1:10 and a Golden Gate reaction was setup with 1x rapid ligase (Roche), 10 units of BbsI enzyme ...
-
bioRxiv - Neuroscience 2022Quote: ... The cells were washed in chilled 1X PBS and subjected to dounce homogenization in lysis buffer (5mM HEPES, 85mM KCl, 1% IGEPAL CA-630 in 1X PBS, 1X Roche Complete protease inhibitors ...
-
bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 1 mL Equilibration Buffer B containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche) and 0.2 mg/mL lysozyme ...
-
bioRxiv - Plant Biology 2022Quote: ... with Horse Radish Peroxidase (HRP)-conjugated antibody (HRP-conjugated anti-HA antibody, 1/1000 [50 mg/mL], cat. no. 12013819001, Roche; HRP-conjugated anti-FLAG antibody ...
-
bioRxiv - Molecular Biology 2022Quote: ... Sonicated samples were centrifuged and the supernatant (soluble fraction) was incubated for 1 h at 4°C with beads crosslinked to anti-GFP antibody (11814460001; Roche) or to M2 anti-FLAG antibody (F1804 ...
-
bioRxiv - Microbiology 2024Quote: ... Different dilutions of the enzyme preparation were assayed for 1 hour and analyzed with a luciferin-luciferase coupled reaction (76) using the CLSII-Bioluminiscent ATP Assay Kit (Roche).
-
bioRxiv - Immunology 2023Quote: ... The cell pellets were treated with lysis buffer (50 µg/mL digitonin, 75 mM NaCl, 1 mM NaH2PO4, 8 mM Na2HPO4, 250 mM sucrose, and Roche cOmpleteTM protease inhibitor cocktail ...
-
bioRxiv - Plant Biology 2023Quote: ... Detection of alkaline phosphatase was carried out for ∼1 h using NBT/BCIP ready-to-use stock solution (La Roche) diluted in 1× washing buffer.
-
bioRxiv - Plant Biology 2024Quote: ... The transformants were screened on MS plants with 1% sucrose and 0.8% agar containing 40mg/L hygromycin (Roche, no. 10843555001). For each construct ...
-
bioRxiv - Biochemistry 2024Quote: ... the proteins were transferred to a PVDF membrane and immunodetected with an anti-Myc-HRP antibody (Myc-HRP, 1:5000, Roche). The remaining 10% of the immunoprecipitated samples together with 50 μg of the input were subjected to the same procedure but probed with an anti-GFP antibody (JL-8 ...
-
bioRxiv - Bioengineering 2024Quote: cDNA from cells was diluted 1:10 and qPCR was performed in triplicate using LightCycler 480 SYBR Green I Mastermix (Roche). Samples containing primers specific for lmna ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were disrupted in 1% NP-40 lysis buffer (140 mM NaCl, 10 mM Tris-HCl pH 8, 1% NP-40) supplemented with proteinase inhibitors (Roche). Proteins were separated by electrophoresis (SDS-PAGE) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Membrane lysis was performed in 5Lml LB1 (50LmM HEPES pH 7.5, 140LmM NaCl, 1LmM EDTA, 10% glycerol, 0.5% 1% IPEGAL-CA630, 0.25% Triton X-100, and Roche protease inhibitors 11836170001) for 10 min at 4 °C with rotation ...
-
bioRxiv - Microbiology 2024Quote: ... The gDNA levels corresponding to the viral genes and the housekeeping wasp gene (elongation factor (ELF-1)) were determined using the LightCycler 480 System (Roche). The cycling conditions involved heating at 95°C for 10 min ...
-
bioRxiv - Microbiology 2024Quote: HeLa cells treated with or without CsA were infected with HIV-1 (10 ng p24) that had been treated with 100 U/ml of DNase I (Roche) for 1 h at 37° C ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Cells were resuspended in lysis buffer (100 mM Pi pH 7, 100 mM NaCl, 1 mM DTT, C0mplete protease inhibitor tablet, Roche) and lysed by sonication for 8 min (Vibracell ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells were harvested and stored at -80°C in ICD lysis buffer (300 mM NaCl, 50 mM Tris/HCl, 1 mM EDTA, 1x Protease Inhibitor (Roche), pH 7.4) ...
-
bioRxiv - Pathology 2024Quote: ... cDNA product was 1 to 40 diluted and real-time PCR was performed using FastStart SYBR Green Master Mix (Roche) on QuantStudio 5 (ThermoFisher ...
-
bioRxiv - Molecular Biology 2024Quote: ... 15 µl of IPed proteins and 10 µl of WCE were submitted to SDS-PAGE and analyzed by immunoblotting using anti-HA antibody (1:250, ref.12013819001, Roche), anti-Flag antibody (1:500 ...
-
bioRxiv - Microbiology 2024Quote: ... The bacterial pellets were then resuspended in 20 ml of lysis buffer pH 8.0 (50 mM NaH2PO4, 300 mM NaCl, 5 mM Imidazole) containing 1× cOmplete EDTA-free protease inhibitor cocktail (Roche, Germany) and 1 mg/ml lysozyme ...
-
bioRxiv - Cell Biology 2023Quote: Frozen liver tissues that were crushed using a Multi-beads Shocker (Yasui Kikai, Japan) or cultured cells were dissolved in RIPA buffer containing 1× cOmplete proteinase Inhibitor Cocktail (Roche). Protein concentration in the lysates was determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2023Quote: ... and collected in lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor [#11873580001, Roche] in PBS). Chromatin was sonicated with a Covaris E220 sonicator (140PIP ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were then resuspended in cold lysis buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 5 mM MgCl2, 1 mM EGTA, Protease Inhibitor (Roche, 04693159001)) ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The final pellet was resuspended in 750 uL nuclear lysis buffer (10 mM EDTA, 1% SDS, 50 mM Tris-HCl at pH 8, 1x cOmplete protease inhibitor cocktails (Roche)) ...
-
bioRxiv - Developmental Biology 2024Quote: ... blocked in MAB-Buffer2 (20% sheep serum, MAB-Buffer1) for 1h and incubated with anti-DIG-AP antibody (1:4000, Roche) in MAB-Buffer2 overnight at 4 °C.
-
bioRxiv - Molecular Biology 2024Quote: ... whole cell lysate samples were prepared by resuspension and trituration of cell pellets in a lysis buffer (20 µM Tris-HCl pH 7.4, 1 µg/mL DNaseI, 1X cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche)) ...
-
bioRxiv - Neuroscience 2023Quote: ... the supernatant replaced by 1ml PBS-Glucose supplemented with 50 µl Collagenase/Dispase (Worthington CLS-1 345U/mg; Dispase II Roche 1.2U/mg ...
-
bioRxiv - Synthetic Biology 2023Quote: ... cell pellets were resuspended in 10 ml cold TES buffer (50 mM Tris pH 8.0, 20% Sucrose, 1 mM EDTA) supplemented with EDTA-free complete protease inhibitor (Roche, Switzerland) and incubated at 4 °C for 45 mins ...
-
bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked with freshly prepared blocking solution (100 mM maleic acid, 150 mM NaCl, pH 7.5, 1% (wt/vol) blocking reagent (Roche, 11096176001)) for 30 min ...
-
bioRxiv - Immunology 2023Quote: ... lung and liver tissues were mechanically disrupted into small pieces and enzymatically digested in 2ml of digestion buffer (1 mg Collagenase D (Roche) and 0.2 mg DNase I (Roche ...
-
bioRxiv - Microbiology 2023Quote: Cells were washed in PBS and harvested in lysis buffer (1% NP-40, 2mM EDTA, 10% glycerol, 150mM NaCl, 50mM Tris-HCl, protease inhibitor (Roche complete mini EDTA-free protease inhibitor) ...
-
bioRxiv - Developmental Biology 2023Quote: Five whole embryos or ten explants were lysed in 1% NP-40 supplemented with protease inhibitors (Complete Mini, EDTA-free tablet (Roche), Leupeptin (Roche) ...
-
bioRxiv - Pathology 2023Quote: ... The preparations were washed with buffer 1 and incubated for 2h at 37°C with an anti-digoxigenin antibody conjugated to alkaline phosphatase (1:200 in blocking solution; 11093274910, Roche). Next ...
-
bioRxiv - Molecular Biology 2023Quote: ... The frozen cell pellets were resuspended in lysis buffer (50 mM TRIS pH 7, 150 mM NaCl, 1 mM EDTA, Roche’s complete Protease inhibitors ...