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Citations for Roche :
6801 - 6850 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... cells were lysed for 30 min on ice in lysis buffer supplemented with fresh 1 x protease inhibitor cocktail (Roche). Unless otherwise indicated ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... The lungs were perfused through the pulmonary artery and inflated via the airway with DMEM containing 1 mg/ml Collagenase/Dispase (Roche), 3 U/ml Elastase (Worthington) ...
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bioRxiv - Neuroscience 2024Quote: ... co-cultures were treated every 48 h from the start of co-culture with either 1 µM LGMN inhibitor (Roche) or a DMSO control for three weeks.
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bioRxiv - Molecular Biology 2024Quote: ... and twice with TE wash buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 1x Roche protease inhibitor mixture).
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bioRxiv - Microbiology 2023Quote: ... Nuclear pellets were resuspended in shearing buffer (0.1% SDS, 1 mM EDTA, 10 mM Tris-HCl pH 7.5, 1X Roche protease inhibitor, and 1X Roche phosphatase inhibitor) and transferred into 130 μl Covaris tubes (Covaris ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed with 1x PBS then lysed in 1% Triton lysis buffer or 1x RIPA lysis buffer supplemented with Complete Mini Protease inhibitor cocktail (Roche). The concentration of protein was then assessed via BCA and samples were normalized and added to sample buffer supplemented with 10% BME before being run on an SDS-PAGE gel (Invitrogen NW04125BOX) ...
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bioRxiv - Microbiology 2023Quote: ... and the murine J774A.1 (ATCC TIB-67) macrophages was assessed using a lactate dehydrogenase (LDH) cytotoxicity detection kitPLUS (Roche), which measures the release of the LDH enzyme in the growth medium ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed with sonication in lysis buffer (50mM Hepes, 500mM NaCl, 1% Tween20, 5% Glycerol, half a tablet of cOmplete EDTA-free protease inhibitor cocktail [Roche]). The suspension was sonicated at 150J in intervals of 1.5secs for 20 sec total in ice ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested after 48 hours and lysed in 50 mM Tris pH 7.5, 150 mM NaCl, 1% Triton X-100, protease inhibitor (cOmplete™, Mini, EDTA-free (Roche)) ...
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bioRxiv - Neuroscience 2023Quote: ... The larvae were then incubated overnight at 4°C with anti-Dig-AP (1:2000 in 5% normal goat serum, 11093274910, Roche) and washed before detecting alkaline phosphatase using NBP/BCIP(Roche ...
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bioRxiv - Biophysics 2023Quote: ... 5% d8-13C depleted glycerol, 1 mM EDTA, 5 ug/mL of aprotinin and leupeptin and 100 µg/mL Roche protease inhibitor cocktail ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNAs were obtained by reverse transcription of 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by quick wash with PBS and lysis with 400 µM of lysis buffer (50LJmM Tris pH7.5, 150LJmM NaCl, 1% Triton-X supplemented with 2x protease inhibitor cocktail (Roche, 11836145001), 1x phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Screening for repeat expansions in RFC1 was achieved by a combination of short-range repeat-spanning end-point PCR (Supplementary Table 1) with 2X Faststart PCR mastermix (Roche) and by Repeat-Primed PCR using 2X Phusion Flash High-Fidelity PCR Mastermix (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... washed and lysed in a protein extraction buffer (10mM TrisHCl pH7.5, 5mM EDTA, 150mM NaCl, 30mM Na2HPO4, 50mM NaF, 10% glycerol, 1% NP40, 1X cOmplete (Roche #11873580001), 1X PhosSTOP (Roche #4906845001) ...
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bioRxiv - Neuroscience 2023Quote: ... 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, protease inhibitor cocktail [Roche], and PhosStop [Roche]; pH 7.5) and sonicated for 30 s ...
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bioRxiv - Neuroscience 2023Quote: Neurons were lysed with 200 μl SDS lysis buffer (1% SDS with protease inhibitor cocktail tablet; Roche, cat. no 04693159001) per well in 6-well plates and scraping ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Prehybridization for 60 min and overnight hybridization were carried out with a DIG-labeled probe (1 µg) at 65 °C with DIG Easy Hyb Granules (Roche). A chemiluminescent assay was used to visualize the probe on the membrane ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were re-suspended in 500 µL of lysis buffer (PBS containing 1 % Triton-X 100 and complete protease inhibitors [Roche]). DAPI was added to lysis buffer (final concentration 5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed twice with PBS and lysed using a lysis buffer (50mM Tris, pH7.4; 150mM NaCl; 1% NP-40) supplemented with protease (Roche, #11836153001) and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of the reverse transcrip-tion mix from the Titan One Tube RT-PCR System kit (Roche, Switzerland) was added ...
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bioRxiv - Neuroscience 2023Quote: ... then lysed on ice for 15 minutes in non-denaturing lysis buffer (150 mM KCl, 10 mM MgCl2, 5mM HEPES, 1% IGEPAL) supplemented with protease inhibitors (Roche Complete EDTA-free ...
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bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP 10 % v/v glycerol) supplemented with 4x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Developmental Biology 2022Quote: ... before being incubated in TNTw/block for 1 hour followed by an ON incubation with anti-DIG or anti-Fluo horseradish peroxidase (Roche). Post-antibody washes and the TSA reactions were repeated as for the first probe ...
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bioRxiv - Cell Biology 2022Quote: ... Thawed pellets were resuspended with 200-300 µL native lysate buffer (1% NP-40 with 1X cOmplete protease inhibitor cocktail [SKU, 11836145001, Roche] ...
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bioRxiv - Cell Biology 2022Quote: ... At the completion of the assay islets were lysed in RIPA buffer (Pierce) containing 1 mM PMSF and EDTA-free protease inhibitor cocktail (Roche)) at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: Protein lysates were collected from worms using RIPA buffer (50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, and Roche protease inhibitor tablet ...
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bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and directly lysed in 50 μl of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... at 1:500 for 32 min was used followed with the secondary antibody OmniMap anti-rabbit HRP (760-4311, Roche). Antigen-antibody complexes were revealed with ChromoMap DAB Kit (Roche ...
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bioRxiv - Biochemistry 2022Quote: ... 150 ng of U1 RNA (TMG or NAD cap) and 150 ng of HIV-1 mRNA were mixed with ResoLight dye (1x, Roche) and annealing buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: Mouse pancreata from adult mice (9-14 week old) were perfused with a solution containing 1 mg/mL collagenase-P (Roche) in Hank’s buffered salt solution (HBSS ...
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Cell Biology 2022Quote: ... Both Caco2 and HepG2 cells were transfected with 1 µg of pooled plasmid using X-tremeGENE9 Transfection Reagent (Roche:6365779001) following manufacturer recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... isolated from each cell line was collected by centrifugation (30 000 x g, 4°C, 10 min) and resuspend in 1 x PBS supplemented with protease inhibitors (Roche) to protein concentration 1.5 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: LC-MS-based quantification of methyl-cytosines was performed on 1 μg of DNA degraded to nucleosides with nuclease P1 (Roche), snake venom phosphodiesterase (Worthington ...
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bioRxiv - Genetics 2022Quote: ... 50000 cells were lysed in NPB buffer (PBS containing 5% BSA, 1mM DTT, 0.2% IGEPAL, 1□×_Roche Complete Protease Inhibitor) and incubated on ice for 15 min to isolate nuclei ...
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bioRxiv - Genetics 2022Quote: ... COS-7 cells transiently expressing POPDC constructs with the appropriate epitope tag were lysed using a 1% (v/v) Triton X-100 based lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche). Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: Slides were blocked for two hours at room temperature in blocking buffer composed of 1% Roche blocking reagent (Roche #11096176001) and 20% sheep serum ...
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bioRxiv - Developmental Biology 2022Quote: ... and 0.25 µM forward and reverse primers (Extended Data Table 1) on a LightCycler 480/II 384 (Roche, serial #6073). Four technical replicates were performed per biological replicate qPCR primer set ...
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bioRxiv - Microbiology 2022Quote: ... lacking the predicted signal peptide (amino acids 1-29) was amplified from genomic DNA (isolated using the High Pure Template kit, Roche) by PCR using the Phusion Hot start II High Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: ... Sections after the post-hybridization washing and blocking steps were incubated with peroxidase-conjugated anti-digoxigenin sheep antibody (1:1000; 11207733910, Roche) in TS 7.5 containing 1% Blocking Reagent at 4°C for overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with SAE2-T2A-SAE1 cDNA in the pcDNA5/FRT/TO vector and the Flp-recombinase cDNA in the pOG44 vector at a 5:1 pcDNA5/FRT/TO DNA: pOGG44 DNA ratio using FuGene6 (Roche) at a ratio of 4:1 FuGene (μl) ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed three times with 400 μL wash buffer (25 mM Tris-HCl pH 8, 150 mM NaCl, 1 mM EDTA pH 8, cOmplete protease inhibitor cocktail (Roche), 5% glycerol) ...
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bioRxiv - Microbiology 2022Quote: ... A cell pellet was resuspended in 250 μL Tris-EDTA buffer and 50 μL 30 g L-1 lysozyme (Roche) in Tris-EDTA buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 mM imidazole and 10 % (v/v) glycerol] supplemented with 1 mM PMSF and complete EDTA-free protease inhibitor cocktail (Roche). After centrifugation at 45 000 x g supernatant was filtered (0.45 µm ...