Labshake search
Citations for Roche :
6951 - 7000 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... 1% replacement NP-40, 10 mM EDTA, 25 mM HEPES, 1 mM TCEP, 10 μM Combretastatin, 10 μM Cytochalasin D, Roche Protease Inhibitor Mini EDTA free ...
-
bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by discarding 100 ul from each well and adding 100 ul of solution containing DMEM with 10% FBS and WST-1 reagent (Roche Diagnostics) diluted 1:10 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 3 μL of each primer at 10 μM and 0.5 μL of 1 U/μL Kapa HiFi HotStart DNA polymerase (Kapa Biosystems, Inc, A Roche Company) for a total volume of 25 μL ...
-
bioRxiv - Cancer Biology 2021Quote: ... For CO-IP MS analysis JJN-3 KO and WT cells were incubated in hypoxia for 24 hours before lysis with 4x pellet volume RIPA buffer (1% CHAPS, 50 mM Tris, 150 mM NaCl, Complete protease inhibitor (Roche Diagnostics) and phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1μg of this material was then ligated in a volume of 20 μl with 1 μl of T4 DNA ligase (Roche, 10799009001) at 16°C to generate a randomized library of large fragments ...
-
bioRxiv - Immunology 2021Quote: Single-cell suspensions of tumors were generated by dicing tumors with a razor blade followed by enzymatic digestion for 30 minutes at 37C in medium containing 1 mg/mL Collagenase D (Roche Diagnostics) and 1μg/mL DNase I (ThermoFisher Scientific) ...
-
bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
-
bioRxiv - Genomics 2020Quote: ... This was followed with primary antibody incubation performed with 1:1000 goat anti-SOX17 (R&D) diluted with Antibody Dilution Buffer (Roche Ventana) for 60 minutes at 37°C ...
-
bioRxiv - Genetics 2020Quote: ... and the cDNA was diluted 1/10 for qPCRs using MESA BLUE MasterMix (Eurogenentec, 10-SY2X-03+NRWOUB) on a LightCycler® 480 Instrument II (Roche). A list of primers used can be found in Supplementary Table 7.
-
bioRxiv - Biochemistry 2020Quote: ... Blots were re-probed to detect ZMPSTE24 and Rce1 using rat anti-HA (clone 3F10, Roche cat #11867423001; 1:10000 dilution), and polyclonal rabbit anti-Rce1 [52] (1:2000 dilution) ...
-
bioRxiv - Immunology 2021Quote: ... 1mM PMSF, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1x Roche Complete Protease Inhibitor Cocktail, and 1x Roche Phosphatase Inhibitor Cocktail). Protein concentrations were measured using a BCA kit (Pierce) ...
-
bioRxiv - Biochemistry 2020Quote: ... 150 mM NaCl and 1 mM DTT and incubated at RT in 0.05 mg/mL sequencing grade trypsin (Roche Applied Science). Digestions were stopped with 2X Laemmli sample buffer and placed on ice until analysis ...
-
bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and left to incubate for 24 hours with blocking buffer containing a 1:200 dilution of anti-DIG (Roche, 11207733910) or anti-fluorescein antibody conjugated with POD (Roche ...
-
bioRxiv - Neuroscience 2020Quote: All qPCR reactions were performed in 10 μl volume in triplicates with 1× HOT FIREpol EvaGreen qPCR Mix Plus (Solis Biodyne) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche). Gene expression levels were normalized to HPRT1 mRNA levels in neurons and Cyclophilin B mRNA levels in astrocytes ...
-
bioRxiv - Plant Biology 2021Quote: ... 150mM NaCl] supplemented with 1% Tween® 20 for 30 min before probing the membrane with either rat monoclonal anti-HA antibody (3F10, Roche) or mouse monoclonal ANTI-FLAG® antibody conjugated to HRP (M2 ...
-
bioRxiv - Cancer Biology 2020Quote: ... washed twice in PBS and resuspended in 100 μL annexin V incubation buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 5 mM CaCl2) containing 1% annexin V FLOUS (Roche Molecular Biochemicals) and 500 μg/μl PI stain ...
-
bioRxiv - Plant Biology 2020Quote: ... transferred to a PVDF membrane and immunoblotted with rat monoclonal anti-HA (High Affinity, clone 3F10, Roche, www.roche.com; 1:2000 dilution) and hybridized with peroxidase conjugated goat anti-rat (Polyclonal ...
-
bioRxiv - Microbiology 2021Quote: ... HindIII treatment was performed at 37°C for 1 h in 1x reaction buffer B with 10U of HindIII enzyme per reaction (HindIII, 10656321001, Roche, Switzerland). Mung bean nuclease treatment was performed at 30°C for 30 min in 1x mung bean nuclease reaction buffer with 1U per reaction (Mung Bean Nuclease ...
-
bioRxiv - Plant Biology 2021Quote: ... The digested DNA was separated in a 1 % agarose gel at 50 Volts for 72 hours at 4°C and transferred to a nylon membrane (Roche®) overnight ...
-
bioRxiv - Cell Biology 2021Quote: Transduced MEFs were seeded in triplicate in a 96 well flat bottom tissue culture plate and WST-1 assay (Roche, Switzerland) performed as per manufacturer’s protocol at day 0 ...
-
bioRxiv - Cell Biology 2022Quote: 3T3-L1 adipocytes were washed with ice-cold PBS and harvested in ice-cold HES-I buffer (20 mM HEPES, pH 7.4, 1 mM EDTA, 250 mM sucrose containing protease inhibitors mixture (Roche Applied Science)) ...
-
bioRxiv - Molecular Biology 2022Quote: ... synovial tissues from three patients with RA were minced and incubated with 1 mg/mL collagenase/dispase (Roche, Indianapolis, IN, USA) in phosphate-buffered saline (pH7.2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Biotin-labeled LPA1-HA was detected using streptavidin beads (Pierce) and conjugated anti-HA antibody (3F10 from Roche Diagnostics; 1:1000).
-
bioRxiv - Biophysics 2022Quote: ... embryos were washed (4 X 10 min) in PBTx and blocked in PBT-B (1× PBS, 20% (v/v) western blocking reagent (Roche, 11921673001), 2 mM ribonucleoside vanadyl complex (NEB ...
-
bioRxiv - Cell Biology 2022Quote: ... 148.5 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 1x PhosSTOP Roche, 5x cOmplete protease inhibitor cocktail Roche, 1 mM DTT) using a cell scraper (TPP 99003) ...
-
bioRxiv - Biochemistry 2022Quote: ... one volume of 2X TBS buffer supplemented with 1 mM PMSF and cOmplete EDTA-free protease inhibitor cocktail (Roche Applied Science) was added to one volume of packed worms then added dropwise to liquid N2 to obtain frozen worm ‘popcorn’ ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei pellet was then resuspended and incubated in 1 mL of nuclear lysis buffer (50mM Tris-HCL pH8.0, 100mM EDTA, 1% SDS, 1mM PMSF, SIGMAFAST™ protease inhibitor cocktail, and Roche PhoStop) on ice for 30 min and 3 freeze-thaw cycles to release chromatin ...
-
bioRxiv - Molecular Biology 2022Quote: Cryo-lysed cells were resuspended in lysis buffer (40mM Tris pH 8.0, 200mM sodium citrate) with 1× complete Mini EDTA-free protease-inhibitor solution (Roche Life Science). After centrifugation at 14,000×g for 10 min at 4 °C ...
-
bioRxiv - Microbiology 2022Quote: ... The capsids were suspended in sample buffer (1% [w/v] SDS, 50 mM Tris-HCl, pH 6.8, 1% [v/v] β-mercaptoethanol, 5% [v/v] glycerol, PIs [Roche cOmplete]), and adsorbed to nitrocellulose membranes (BioTrace™ ...
-
bioRxiv - Microbiology 2022Quote: The samples were lysed in Laemmli buffer (1% [w/v] SDS, 50 mM Tris-HCl, pH 6.8, 1% [v/v] β-mercaptoethanol, 5% [v/v] glycerol, bromophenol blue, PIs [Roche cOmplete]). The proteins were separated on linear 7.5 to 12% or 10 to 15% SDS-PAGE ...
-
bioRxiv - Neuroscience 2022Quote: ... brains homogenized in 1xPBS containing 1% SDS and protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail, Roche, Sigma Aldrich, Cat#11873580001) and boiled for five min ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 µg of 330-BFP-CYP3A4-enhancer-R-sgRNA were transfected with X-tremeGENE 9 DNA transfection reagent (Roche 6365809001). After 3-5 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by rabbit anti-Rat (AI-4001, Vector) at 1:500 for 32 min and OmniMap™ anti-Rb HRP (760-4311, Roche). Blocking was performed using casein (760-219 ...
-
bioRxiv - Biochemistry 2022Quote: ... and subsequent addition of 50 µl lysis buffer supplemented with 1 mM PMSF / ml buffer and 22 µl / ml buffer protease inhibitor cocktail (Complete, Roche Diagnostics). The cell samples were boiled for 3 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 10% sheep serum] and incubated with a 1:2000 dilution of alkaline phosphatase-conjugated anti-digoxigenin antibody (Roche Cat# 11093274910) in MABT/Block at 4°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
-
bioRxiv - Neuroscience 2021Quote: ... proteinase K treatment was omitted and color development of alkaline phosphatase activity was carried out for 1 h with nitroblue tetrazolium chloride (Roche Diagnostics) and 5-bromo-4-chloro-3-indolyl phosphate (Roche Diagnostics ...
-
bioRxiv - Molecular Biology 2020Quote: ... nuclei were pelleted by centrifugation at 2500g for 5 minutes and resuspended in 880ul Nuclear Lysis Buffer (50mM Tris HCl, 10mM EDTA, 1% SDS, 1X protease inhibitors (Roche, 04693124001)) ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 M sucrose, 5 mM KCl, 5 mM MgCl2, 0.6% Triton X-100, 0.4 mM PMSF, 1X Roche protease inhibitor cocktail) and homogenized with mixing for 15 minutes at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... The pellet of 100 mL was resuspended in 1 mL cold fractionation buffer (40 mM HEPES, 0.1 mM EDTA, 1x cOmplete™ Protease Inhibitor Cocktail (Roche Sigma)) and the pellet of 1 mL lysed with Bug Buster (pellet sample ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 μM benzyloxycarbonyl (Z)-Leu-Leu-Leucinal (protease inhibitor, synthesized in-house) and Complete EDTA-free Protease Inhibitor (Roche, cat# 05056489001) in 50 mm MES pH 6.5 with or without 10 μM thiorphan (neprilysin-specific inhibitor ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Viral supernatants were prepared by transfecting 293T cells with GFP- or ABCG-targeting shRNAs encoded in pLKO.1 vectors using X-tremeGENE 360 transfection reagent (Roche, Germany) as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... and tissue homogenization was performed using a Bullet Blender in RIPA buffer supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics), 1% phosphatase inhibitor cocktail 2 (#P5726 ...
-
bioRxiv - Genetics 2022Quote: ... Samples were suspended in Laemmli sample buffer supplemented with a protease and phosphatase inhibitor cocktail (PIC; 1 mM PMSF, cOmplete™ Protease Inhibitor Cocktail [Roche, Base ...
-
bioRxiv - Pathology 2022Quote: ... labeling probe (359 bp) was synthesized using primers TiPV-Fq and TiPV-NS1-R (Table 1) and DIG-labeling mix reagent (Roche, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with cold PBS and lysed using buffer A (PBS, 20 mM imidazole, 1% Triton X-100, and a cOmplete® protease inhibitor tablet (Roche). Lysates were clarified for 30 min at 13,000 g at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... the lysed cells were resuspended in LB2 (10 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, Roche cOmplete protease inhibitors) and incubated at 35 rpm for 5 min followed by centrifugation ...
-
Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... sample pellets were resuspended in 200 µL lysis buffer (100 mM triethylammonium bicarbonate buffer (TEAB) with 1% (v/v) sodium deoxycholate (SDC) and 1x protease inhibitor cocktail solution (Roche, Switzerland)) ...
-
bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma], 10X protease inhibitors [Complete tablets, Roche, Indianapolis, IN]). Sucrose gradients for the preparation of lipid rafts were assembled previously described (18) ...