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Citations for Roche :
7101 - 7150 of 7401 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and incubated at room temperature for 30 minutes with HRP-labeled sheep anti-FITC antibody (1/500 in TNB, Roche/Sigma). Following washes in TNT ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were ground in liquid nitrogen and homogenized in 40 ml of NEB1 buffer (10 mM Tris-HCl [pH 8.0], 0.4 M sucrose, 10 mM MgCl2, 5 mM DTT, 0.1 mM PMSF, 1% Roche protease inhibitor cocktail tablet). The homogenate was filtrated through two layers of Miracloth ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were blocked in 10% sheep serum and incubated overnight at 370C with anti-DIG-AP antibody (Roche at 1:1000). Signal detection was done using NBT/BCIP substrate (Roche).
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bioRxiv - Cell Biology 2024Quote: Samples were lysed in ice cold radioimmunoprecipitation assay (RIPA) buffer containing 1:10 PhosSTOP™ (#4906845001, Roche Diagnostics, Indianapolis, IN, USA) and 1:20 cOmplete™ ULTRA Protease Inhibitor Cocktail (#06538304001 ...
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bioRxiv - Genomics 2024Quote: ... heat-induced epitope retrieval was performed by boiling the sections for 48 minutes with Cell Conditioning 1 buffer (DISC CC1 RUO, Roche, 6414575001) followed by incubation with DISC inhibitor (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Then tissue was rinsed three times in ice-cold RPMI-1640 and digested in 5 ml of complete media (RPMI-1640 containing 10% FBS, 1% antibiotic/antimycotic, 5mg/ml DNase I) supplemented with 5 mg/mL Liberase™ (Roche) and 5mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were then washed three times with 1 mL of lysis buffer with EDTA-free protease-inhibitor cocktail (Roche, Indianapolis, IN), and then three times with 1 mL of lysis buffer without NP-40 or protease-inhibitor cocktail ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples subjected to colorimetric signal detection were incubated in anti-Digoxygenin-AP antibody solution (1:4000 dilution; Roche Diagnostics, Mannheim, Germany) overnight while rocking at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... immunoblotting was performed and HA-tagged SopB was detected using a HA-specific antibody (diluted 1:1000, Roche, Cat. No.: 1867423).
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bioRxiv - Microbiology 2024Quote: ... protein extracts from the respective parasite lines at different stages were analyzed by SDS-PAGE and immunoblotting using anti-GFP mAb (1:1000, Roche, 11814460001), anti-APEX2 antibodies (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue was washed two times before either storing at -80°C or immediately lysed in SDS lysis buffer (50mM Tris Tis pH 8.1, 10mM EDTA, 1% SDS, cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) on ice for 15 minutes and subjected to sonication for 4 separate cycles of 30s on and 30s off cycles at high setting (Bioruptor Plus ...
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bioRxiv - Biochemistry 2024Quote: Bacterial cell pellets were resuspended in 50 ml of lysis buffer (50 mM Tris-HCl pH 8, 1 mM DTT, 5% glycerol, protease inhibitor cocktail [Roche 1183615300]). Cells in pellets were lysed by sonication on ice with 10 sec pulses of maximum setting followed by 1 min cooling period ...
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bioRxiv - Pathology 2024Quote: 150 mg wet weight human lung tissue from IPF patients and healthy controls was homogenized in 1 mL ice cold RIPA buffer (defined previously) with complete mini protease inhibitors (Roche 11836153001). Supernatants were collected following centrifugation (10,000 x g for 20 min x 2) ...
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bioRxiv - Pathology 2024Quote: ... and 1 drop of blood was used for direct blood glucose measurements using an Accu Chek Aviva glucose meter (Roche Diagnostics). For 2 of the non-treated and 3 of the anle138b-treated Ob/Ob mice ...
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bioRxiv - Pathology 2024Quote: ... and 1 drop of blood was used for direct blood glucose measurements using an Accu Chek Aviva glucose meter (Roche Diagnostics). During all 3 ISTs of the hIAPP Ob/Ob mice (i.e ...
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bioRxiv - Systems Biology 2024Quote: ... and 10 µL (1 µg) was used as input to a second strand synthesis reaction with 2x KAPA HiFi Master Mix (Roche, #07958935001) and an equimolar amount of primer P276R (Table S5) ...
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bioRxiv - Neuroscience 2024Quote: Human motor neuron lysates were prepared by resuspending 30 million cells in 1 ml of PBS with protease inhibitors added (Roche, 4693132001). Cell suspension was put through freeze thawing cycle thrice to lyse the cells ...
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bioRxiv - Cell Biology 2024Quote: ... The protein samples were separated with SDS-PAGE gel and detected with monoclonal anti-GFP antibody (1:1,000 dilution; 11814460001; Roche, Mannheim, Germany), monoclonal anti-Myc antibody (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ml lysis buffer was made up using 5 ml RIPA buffer with 5 μl 1 M PMSF and 1x EDTA-free protease inhibitor cocktail (Roche, 11836170001). Cells were washed with 1 ml cold PBS before 250 μl lysis buffer was added to each well ...
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bioRxiv - Immunology 2024Quote: ... A total of 100 pmol siRNA was transfected into 1×106 BmN cells using Roch-X gem (Cat:6366236001; Roche, Switzerland). siRNA-NC was used as the negative control ...
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bioRxiv - Plant Biology 2024Quote: ... In total of 1 μg total RNA was used for first-strand cDNA synthesis using Transcriptor First Strand cDNA synthesis kit (Roche, Switzerland) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: 200×106 A2780 cells were resuspended in a 2.5 ml lysis buffer (1 ml protease inhibitor 25x (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Roche, Switzerland), 1.5 ml 1x PBS) ...
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bioRxiv - Genetics 2024Quote: ... The resultant pellet was homogenized in three volumes times the heaviest sample of 1% Triton™ X-100 in TBS plus complete protease and phosphatase inhibitors (Roche) and centrifuged at 186 000 g at 4°C for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were resuspended in 900 µL of SDS Lysis buffer (50mM Tris pH 8.1, 10mM EDTA, 1% SDS, cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, #cat 05892791001) and sonicated for four cycles of 10 minutes (30 seconds ON 30 seconds OFF ...
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bioRxiv - Biophysics 2024Quote: ... The PCR cycle was determined for each library using 1/10 of the adaptor-ligated DNA as the template with the KAPA SYBR FAST qPCR Master Mix (Roche, KK4603) by taking the Ct that reaches 1/2 of the maximum intensity and subtracting 2 cycles (Ct -2 cycles) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5% (w/v) DOC and 0.1% (w/v) SDS) in the presence of protease (1:40 cOmplete™ Protease Inhibitor Cocktail (Roche, #11836145001)) and phosphatase inhibitors (1:100 Phosphatase inhibitor cocktail 2 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... slides were blocked with 5% lamb serum diluted in TBST and incubated with an anti-digoxigenin antibody conjugated to Alkaline phosphatase (1/2000, Roche, 11093274910) overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was blocked in 5% milk in PBS with 0.1% Tween 20 (PBS-T) for 1 h at room temperature (RT) before being probed with either rat anti-HA-tag antibody (Roche, 11867423001) diluted 1:1000 in 5% milk in PBST ...
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bioRxiv - Biochemistry 2024Quote: ... 105 g cell pellet was collected and resuspended in 1.5X pellet volume of lysis buffer (50 mM Tris-HCl, pH 8.0, 1 mM EDTA, Protease inhibitor Roche cOmplete mini tablet) and lyzed by French press (1200 Psi.) ...
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bioRxiv - Biochemistry 2024Quote: ... were harvested by centrifugation and resuspended in 25 ml lysis buffer (20 mM Tris-Hcl, pH 7.5 + 200 mM Nacl + 1 mM DTT + 5% Glycerol) and protease inhibitor (Roche, Basel, Switzerland). The suspended cells were disrupted by sonication and then cell debris was separated by centrifugation at 12,000 rpm for 45 min.
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bioRxiv - Cell Biology 2024Quote: ... Samples were analyzed by standard SDS-PAGE and Western blotting using the following primary antibodies: anti-c-Myc (1:1000, Roche 11667149001), anti-HA (1:1000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Binding reactions were carried out in wash buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM DTT, EDTA-free protease inhibitors Roche, 2.5 % glycerol) in a total volume of 15 μL with purified protein and 77.3 nM DNA ...
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bioRxiv - Developmental Biology 2024Quote: ... The DIG hapten was detected by incubating the samples in a 1:4,000 dilution of anti-DIG-rhodamine antibody (Roche, Basel, Switzerland) in PBS-T for 90 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were amplified with target-specific primers (see Supp. Table 1 for primersequences) using a LightCycler 480 II real time PCR system (Roche Diagnostics) as previously described (22).
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were dissociated by incubation in DPBS (without Mg2+ or Ca2+) for 20 min at 37°C and then in 1:200 Liberase TH (Roche, Basel, Switzerland) in DPBS for 20 min at 37°C ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... Yeast pellets were resuspended in 2 x 500 μl of ice-cold lysis buffer (50mM Hepes, 150mM NaCl, 1% NP-40, 1mM EDTA) containing protease inhibitors (complete Protease Inhibitor Cocktail, Roche Life Sciences), 2 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... ESCs or HEK293T cells were harvested and lysed in TEN buffer (50 mM Tris-HCl, 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 0.5% Na-Deoxycholate, supplement with Roche cOmplete Protease Inhibitor). The lysates were quantified by the Bradford method and equal amount of proteins were loaded for Western blot assay ...
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bioRxiv - Neuroscience 2021Quote: ... Beads with cells were washed twice in 1 ml Digitonin Buffer (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Roche cOmplete™; 0.05% digitonin), and resuspended in 50 µl Digitonin Buffer with addition of 2.5 µl CUTANA pAG-MNase (20x stock) ...
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bioRxiv - Microbiology 2021Quote: ChIP followed by qPCR or Illumina sequencing was performed as described58 using the 3F10 rat anti-HA antibody (1:500; Roche no. 11867423001) to immunoprecipitate HA tagged AP2-HS ...
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bioRxiv - Molecular Biology 2021Quote: ... MGC803 cells were harvested and used to prepare lysate with cell lysis buffer with 1× Protease Inhibitor Cocktail (REF04693159001, Roche, Mannheim, Germany). The lysate was incubated with Glutathione Sepharose beads (20 μL for one group ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were made from 1∼2 mL packed worms in lysis buffer (25 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 10% glycerol, 1x cocktail of protease inhibitors from Roche (Complete, EDTA free), 40 mM NaF ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The final PBMC pellet was taken up in 200 μl lysis buffer (PBS + 1% Triton-X100 + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tissues were homogenized in 1 ml N-PER (brain) or T-PER (other organs) + Complete Mini protease inhibitor cocktail tablet (Roche, Mannheim, Germany) in FastPrep Lysing Matrix D tubes on a FastPrep homogenizer ...
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bioRxiv - Cell Biology 2022Quote: ... minced into ∼1-mm pieces and sequentially digested twice for 30-min in endotoxin-free collagenase (Liberase; Roche Diagnostics GmbH, Mannheim, Germany) to obtain osteocyte-enriched bone samples ...
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bioRxiv - Genomics 2022Quote: ... 0.1% NP-40 and 5 mM β-mercaptoethanol) supplemented with fresh protease inhibitors (AEBSF, Complete™ EDTA-free Protease Inhibitor Cocktail, Roche). Emulsions were snap-frozen in droplets in liquid nitrogen and cells subjected to cryogenic grinding using a Ball Mill MM 400 (5 cycles of 3 minutes at 20 Hz) ...
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bioRxiv - Microbiology 2020Quote: ... 1 mL digestion mix (4.8 mg/ml Collagenase IV; Worthington Biochem, and 200 μg/ml DNase I; Roche Diagnostics, in 1x PBS) was added after which the tissue was incubated at 37°C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and amplified using a reporter specific forward primer with illumina sequencing adaptors and a reverse primer binding the partial illumina read 1 sequence with the remaining sequencing adaptors using Kapa HiFi HotStart DNA Polymerase (Kapa Biosystems, #KK2601) using 18X cycles for GFP and 23X for Firefly reporter ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Cells in 6-well plates were lysed with RIPA lysis buffer containing 1mM PMSF and 1% phosphatase inhibitor cocktail (PhosSTOP, Roche group, Swiss). The supernatant was collected after centrifuging at 14,000 rpm for 15 min at 4℃ ...