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Citations for Roche :
6151 - 6200 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and centrifuged at 4000 rpm for 1 min at 4°C and run on the LightCycler 480 II (Roche) using the steps outlined in Supplemental Table 3.
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with cold PBS before resuspending in ChIP shearing buffer (50 mM Tris HCl pH 8.0, 1% SDS, 10 mM EDTA with protease and phosphatase inhibitors, Roche). Samples were sonicated using a Covaris ME220 for 7.5 minutes using the following sonication conditions ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were co-lysed together in lysis buffer (40mM Tris-HCl pH 7.5, 500mM NaCl and 1 mM DTT) with 100 mM Pefabloc (Roche) and protease inhibitor cocktail (Roche cOmplete EDTA-free ...
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bioRxiv - Cancer Biology 2024Quote: ... and MAZ (1:25, Immunoway Cat# YT2669) was performed using the Ventana BenchMark ULTRA instrument (Ventana/Roche, AZ, USA) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... and the remaining cell pellet was lysed at a final concentration of 1.25×106 cells/mL in lysis buffer (1% NP40-PBS containing protease and phosphatase inhibitors (Roche)) and stored at -20°C until further analysis.
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bioRxiv - Biochemistry 2024Quote: ... resuspended in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 20 mM Imidazole, 1 mM TCEP, and protease inhibitors from Roche), and lysed by several passes through a microfluidizer (LM20 Microfluidizer ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody-bound beads were washed in BSA-PBS (0.5%) before the addition of pre-cleared lysate in 1% Triton with protease inhibitor (Roche). Following rotation overnight at 4 °C the beads were washed in RIPA (50 mM HEPES pH 7.6 ...
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bioRxiv - Cell Biology 2024Quote: ... islets were preincubated for 1 hour in KRBH buffer containing 0.1% (w/v) bovine serum albumin (BSA) (10775835001, Roche) and 3 mM glucose before incubation with 11 mM glucose ± 100 nM GLP-1RAs in KRBH buffer in a shaking 37°C water bath (80 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were harvested and resuspended in lysis buffer (50 mM HEPES pH 8.0, 300 mM NaCl, 1 mM TCEP, supplemented with Roche EDTA-free cOmplete protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2024Quote: Equal amounts of total RNA (1 µg) were reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with random hexamer primers for one hour at 50°C ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclear pellet was washed once with 3mL ST buffer and then twice with 3mL FANS buffer (1% BSA, 1mM DTT, and 0.2U/μL RNAse inhibitor (03 335 402 001, Protector RNAse inhibitor, Roche) in RNAse free DPBS (AM9624 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacteria pellets were resuspended in Lysis Buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1 mM DTT, 0.1% Triton-X, and protease inhibitors, cOmplete Mini, EDTA-free, Roche, #4693159001). Next ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from 3 million HEK293T cells or 0.75 million primary neurons using TriPure reagent (Roche) and purified using RNeasy MinElute Cleanup Kit (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Dissected hippocampi were dissolved in lysis buffer (1M Tris-Cl, pH 7.5; 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001 ...
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bioRxiv - Microbiology 2020Quote: ... Sections were stained with NBT (Nitroblue tetrazolium) and BCIP (1,5-bromo-4-chlooro-3-indolyl-phosphate, Roche, Switzerland) in developing solution at 37°C for 2 hrs ...
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bioRxiv - Biophysics 2020Quote: ... The 3′ end of the 1,882-nt DNA handle was labeled with dig-ddUTP using terminal transferase (Roche), and the 798-nt DNA handle of the transcript was functionalized with biotin on the 5′ end of the PCR primer ...
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bioRxiv - Cell Biology 2022Quote: ... The sonicated sample was layered over 3 ml of S3 solution (0.88 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) in a new Falcon tube and centrifuged at 3000 × g for 10 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended pellet was layered carefully over 3 ml of S2 solution (0.35 M sucrose, 0.5 mM MgCl2, 1x Complete protease inhibitor cocktail (Roche)) and centrifuged at 1430 × g for 5 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Positive control embryos were incubated in polymerase chain reaction buffer containing 3 U/ml DNase I recombinant (Roche) for 1 h at 37°C before adding the TUNEL reaction mix ...
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bioRxiv - Microbiology 2022Quote: ... purified GST-RomA was incubated with equal amounts of HIS6-LphD or HIS6-LphD Y392F in protein binding buffer (25 mM Tris pH 8.0, 140 mM NaCl, 3 mM KCl, 0.1% NP40 with protease inhibitors (ROCHE)) overnight at 4°C ...
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bioRxiv - Pathology 2020Quote: C-reactive protein was measured using the Tina-quant C-Reactive Protein Gen.3 reagent (Roche, Basel, Switzerland) designed to achieve very high sensitivity ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were incubated at 37°C for 3-4 h before adding DNase I (0.1 U/µl) (Roche) and incubating for another 30 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: Cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.6, 150 mM NaCl, 3 mM MgCl2, 10% glycerol, 0.5% NP-40, cOmplete EDTA-free Protease Inhibitors [Roche]) and then clarified by centrifugation at 21,000 x g for 10 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and single-cell suspensions of lung mononuclear cells were prepared by Liberase Blendzyme 3 (70 ug/ml, Roche) digestion containing DNaseI (30 µg/ml ...
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bioRxiv - Genetics 2022Quote: ... Precise knock-in of 3’ homologous arm was evaluated by PCR using KAPA2G Fast HotStart ReadyMix (KAPA Biosystems) with a primer set (5’-CCACTAGTTCTAGAGCGGC-3’ and 5’-GAACTGTTGGCTACTAACACTAATACTG-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were then prepared from 3 ng of CUT&RUN DNA with the Kapa HyperPrep Kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... The DNA probes were 3′ end-labelled using digoxigenin (DIG)-11-ddUTP and terminal transferase (Roche, Basel, Switzerland). Binding of TFRs to DNA was analyzed using the DIG Gel Shift Kit ...
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bioRxiv - Plant Biology 2024Quote: ... and the reducing agent 1,4-dithiothreitol (DTT, 10 mM; CAS#: 3483-12-3, Roche Diagnostics GmbH, Basel, Switzerland). The algae were incubated in ASW in the absence or presence of either one of the reagents for 30 min in the dark prior to the high light treatment.
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 3 min with 200 μl of cold extraction buffer containing 20 mM Tris-HCl (Roche) pH 6.8 ...
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bioRxiv - Microbiology 2024Quote: ... and PcAEV5-3’ (nt 11,547-12,331) were prepared with specific primer pairs (Table S1) using PCR DIG Labeling Mix (Roche). Riboprobes were also prepared using the DIG RNA Labeling Kit (SP6/T7 ...
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bioRxiv - Immunology 2024Quote: ... colonic biopsies (n=3-4 per patient) were enzymatically digested using 0.5 Wünsch units/ml Liberase TM (Roche) and DNase I at 10µg/ml at 37°C (a protocol previously optimized in our lab)(43) ...
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bioRxiv - Immunology 2024Quote: ... Mouse lungs were excised and lightly homogenized in HEPES buffer containing Liberase Blendzyme 3 (70 μg/ml; Roche) and DNaseI (30 μg/ml ...
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bioRxiv - Physiology 2024Quote: ... then diluted 10x in protein lysis buffer (3% SDS, 50 mM HEPES pH 8.5, 75 mM NaCl + Roche complete cocktail of protease inhibitors) ...
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bioRxiv - Cell Biology 2024Quote: ... the glutathione beads were washed 3 times with 1X MAPK Lysis Buffer with 1X protease inhibitor (Complete, Roche). Proteins bound were eluted using 1xSDS loading buffer and boiled at 70°C for 10 minutes before SDS-PAGE using a stain-free gel (1610183 ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... 2 mM benzamidine and cOmplete protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany)) and incubated with zymolyase 20T for ∼20min at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... resuspended in protein resuspension buffer (2% SDS, 10 mM NaF, 1x Roche cOmplete Mini proteinase inhibitor cocktail ...
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bioRxiv - Microbiology 2021Quote: ... 10 mM Tris(2-carboxyethyl)phosphine) supplemented with phosphatase inhibitor cocktail (Roche) followed by heating to 100°C for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the nuclei were stained with PBS containing 2 μg/ml DAPI (Roche) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM MgCl2) supplemented with a cOmplete Protease Inhibitor Cocktail (Roche Diagnostics) and 1500 units Benzonase (Millipore Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM β-mercaptoethanol and cOmplete™ ETDA-free protease inhibitors (Roche)) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM β-mercaptoethanol and cOmplete™ ETDA-free protease inhibitors (Roche)).
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bioRxiv - Microbiology 2021Quote: ... 0.5% NP-40) with 2 mM PMSF and proteinase inhibitor cocktail (Roche). The supernatant lysates were then incubated with anti-GFP agarose (KTSM1301 ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were stained with 4’6-diamidino-2-phenylindole (DAPI) (Roche, Basilea, Switzerland) and slides were mounted with Fluorsave (Merck) ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mM EDTA) supplemented with a complete protease-inhibitor cocktail (Roche; 11697498001) and disrupted by vortexing with acid-washed glass beads ...
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bioRxiv - Cell Biology 2021Quote: ... and 2 mg/ml lysozyme (Roche, ref. no. 10 837 059 001) and sonicated six times for 30 seconds ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM EGTA) supplemented with protease inhibitors (Complete Mini, Roche, Basel, Switzerland). After clearing (15000×g for 15 min) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM EDTA and cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche, USA). The homogenized samples were centrifuged at 700 g for 10 min to obtain the post-nuclear supernatant (PNS) ...
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bioRxiv - Developmental Biology 2022Quote: ... 1mM EDTA) supplemented with 2× EDTA-free Complete Protease Inhibitor Cocktail (Roche). Protein lysates were collected from supernatant after centrifugation at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... 2 mM MgCl2] supplemented with cOmplete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). After 15 min of incubation on ice ...
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bioRxiv - Plant Biology 2021Quote: ... 2% β-mercaptoethanol) and cOmplete EDTA-free protease inhibitor cocktail (#04693159001, Roche). Water- saturated and Tris-buffered phenol (pH 8 ...