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Citations for Roche :
6001 - 6050 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 0.1 % SDS, 150 mM NaCl, 10 mM EDTA, 20 mM Tris-HCl pH7.5 and complete protease inhibitor mix, Roche) was used to prepare protein lysates ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was incubated with 1 nM DIG (Digoxigenin)-labeled Telomere probes in Hybridization solution (DIG Easy Hyb, Roche) at 42°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... pooled and resuspended in ice-cold lysis buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1 × cOmplete EDTA-free protease inhibitor (Roche), 1 mg/mL DNase and lysozyme ...
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bioRxiv - Biochemistry 2024Quote: ... Frozen cell pellets were lysed in immunoprecipitation lysis buffer (50 mM Tris [pH = 7.5], 150 mM NaCl, 1% IGEPAL, 10% Glycerol) with cOmplete Protease Inhibitor Cocktail (Roche) and PhosSTOP Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... cells were detached using cold PBS and lysed in 350 µl lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100) containing protease inhibitor (Roche) for at least 20 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed twice in ice-cold PBS and resuspended in 600 uL NLB (1xPBS, 300 mM NaCl, 1% TritonX-100, 0.1% Tween-20) freshly supplemented with 1x Complete protease inhibitors (Roche), and 1x PhosSTOP (4906845001 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Neuroscience 2023Quote: ... The heads were crushed on ice using a pestle in 0.5% NP-40 lysis buffer (50 mM Tris, 150 mM NaCl, 10% glycerol, 1 mM EDTA, pH 7.5) supplemented with protease inhibitors (Roche). The resulting lysates were clarified by centrifugation and the supernatants were collected ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... then transferred for 48 hrs in a solution of mouse anti-synaptophysin (1:20, Roche Applied Science, Indianapolis, IN). Sections were then washed in PBS-t 3x10 min ...
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bioRxiv - Molecular Biology 2024Quote: Cells were harvested and protein was extracted using lysis buffer (20mM HEPES pH7.4, 2mM MgCl2, 40mM NaCl and 1% Triton-X) containing complete mini EDTA protease inhibitor (Roche) at 1 in 50 concentration ...
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bioRxiv - Molecular Biology 2024Quote: ... The crude nuclear pellet was resuspended in nuclear lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, and Complete protease inhibitor; Roche) and sonicated with a Branson sonifier (VWR ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell viability was determined by using the WST-1 assay according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). After removing the cell supernatants for cytotoxicity measurements ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and 0.1% Triton X-100 supplemented with 1 mM PMSF and one Complete Protease Inhibitor tablet (EDTA-free; Roche), and then centrifuged at 13,000 × g for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... Bacterial pellets were harvested by centrifugation and resuspended in 50 mL of lysis buffer (20 mM Tris pH 7.5, 500 mM NaCl) to which 1 protease inhibitor tablet was added (Roche). Bacterial cells were lysed by sonication with 10 s pulses for 10 min and cell debris was removed by centrifugation at 25,000 x g for 40 min and cleared cell lysate was filtered through 0.45 μm filters ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Nonidet P-40) supplemented with cOmplete Mini EDTA-free protease inhibitor cocktail tablets (1 tablet/10ml) (11836170001, Roche) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Single cell suspensions were obtained from dissected muscles mechanically disrupted and enzymatically digested at 37 °C for 1h using DMEM 1% P/S medium containing 0.2% collagenase B (Roche) and Calcium dichloride (CaCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... Mixed-stage transgenic worms expressing ectopic GFP::EGO-1 were collected and resuspended in equal volumes of 2x lysis buffer (50 mM Tris-HCl [pH 8.0], 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche ®cOmplete EDTA-Free Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... target proteins were detected by using primary antibodies of mouse monoclonal anti-GFP antibody (1: 2000; Roche, Cat. 11814460001), mouse anti-mCherry (1:5000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonadal tissues were washed three times in 0.2× SSC at 50 °C for 15 min each and transferred to 4× SSC containing 1% blocking reagent (Roche) for 1 h at RT ...
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bioRxiv - Genomics 2023Quote: ... The cell pellet was resuspended with ice-cold Galbraith’s buffer amended with bovine serum albumin (BSA, 0.05% w/v) and cOmplete protease inhibitor (1×, Roche 11697498001), lysed by repeated pipetting ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication in 50 mL lysis buffer (20 mM Tris pH 8.5, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 2x Roche protease inhibitor tablets ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... coli were harvested and disrupted by sonication in the lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP40) supplemented with protease inhibitor cocktail (cOmplete, Roche). After centrifugation at 40000 g for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with horseradish peroxidase (HRP)-conjugated anti-DIG (Roche Applied Science cat#11207733910, 1:500) and anti-GFP (Aves Labs cat#GFP-1010 ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) (10 mL/g pellet weight) supplemented with DNAse and 1 cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 2 L of culture ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were then incubated with the anti-myc antibody (11667149001 9E10 mAb from Roche; 1:250 in blocking buffer) for two hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The Ni-NTA agarose was rotated for 30 min at 4°C with 10 mL of elution buffer (50 mM Na-phosphate buffer pH 8.0, 500 mM NaCl, 1.4 mM β-mercaptoethanol, protease inhibitors [1 Roche Mini EDTA-free tablet] ...
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bioRxiv - Microbiology 2023Quote: ... The nuclear pellets were resuspended in nuclear lysis buffer (50mM Tris-HCl pH 8.0, 10mM EDTA, 1% SDS, 1mM PMSF, cOmplete Protease Inhibitor Cocktail (Roche)) and vortexed at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were next rinsed 3x 15 minutes in room temperature TBST (Tris-buffered saline [pH 8.5] with 1% Tween 20) and blocked for two hours in 20% DIG buffer (Roche) in TBST.
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bioRxiv - Cancer Biology 2023Quote: ... FFPE slides were steamed for 48 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were suspended in ice cold lysis buffer (150 mM NaCl, 1% Triton X-100, 50 mM Tris-HCl pH 8.0) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 million cells were suspended in 500 μl SDS lysis buffer (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1% SDS, protease inhibitor (Roche)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue slides were steamed for 32 minutes in Cell Conditioning 1 (CC1) solution (Roche Diagnostics, Cat. No. 950-500) for antigen retrieval ...
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bioRxiv - Microbiology 2023Quote: ... for 10 min and resuspended in lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris pH 8.1) supplemented with protease inhibitors (Roche) and incubated on ice for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Cells were resuspended in cold homogenization buffer (250 mM sucrose, 250 mM HEPES/KOH pH 7.4, 1 mM EDTA) supplemented with cOmplete protease inhibitors (Roche) and lysed by Dounce homogenization on ice ...
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bioRxiv - Neuroscience 2023Quote: ... 7.5×105 of S2 cells were lysed in Lysis Buffer (50 mM Tris at pH 6.8, 150 mM NaCl, 1% NP40, protease inhibitor cocktail [Roche]), immunoprecipitated with 1 μg of GFP antibody (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... washed once in PBS and lysed for 15–20 min on ice with lysis buffer A (50 mM Tris, pH 7.8, 1% NP-40, 150 mM NaCl) containing protease inhibitors (Complete, Roche), 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... washed with 1 x PBS and resuspended in 100 μl of SoTE buffer (10 mM Tris-HCl pH 8, 1 mM EDTA, 0.15 % Triton X-100, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Genetics 2023Quote: VSMCs and myofibroblasts were scraped in phosphate-buffered saline (PBS) supplemented with protease inhibitor cocktail (1:100, Roche, 11836145001) and phosphatase inhibitor cocktail (1:100 ...
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bioRxiv - Immunology 2022Quote: ... were lysed in 400 μl lysis buffer in urea buffer (8 M urea, 50 mM Tris pH 8.0, 1 mM TCEP) with protease (cOmplete mini EDTA free, Roche) and with phosphatase inhibitors (PhosStop ...
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bioRxiv - Microbiology 2022Quote: ... a 1% agarose gel block embedded with enterococci was treated with lysozyme solution (10 mg/mL; Roche, Basel, Switzerland) at 37 °C for 6 h ...
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bioRxiv - Neuroscience 2022Quote: ... rat colon was homogenized in lysis buffer using 1 mm glass beads (Thistle Scientific) in a MagnaLyser system (Roche). The samples were filtered through a binding column ...