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Citations for Roche :
6051 - 6100 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM TCEP and 10 % glycerol) supplemented with 2x EDTA-free protease inhibitor cocktail tablets (Roche, cat. No. 11836153001) and 4 μg/ml DNase I ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: all the samples were lysed with High Salt Buffer (50 mM Tris pH 7.5, 300 mM NaCl, 1% Triton X-100, Protease inhibitors (Roche), Phosphatase inhibitors Halt cocktail ...
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bioRxiv - Biochemistry 2022Quote: ... 1-2×108 cells were lysed by mechanical lysis with glass beads in native lysis buffer (150 mM Tris/HCl pH 7.5, 50 mM NaCl, 1 mM PMSF and Complete protease inhibitors (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.34 M Sucrose, 10% Glycerol, 1 mM DTT, 0.1% TritonX100 added with 1x PI and 1x Phosphatase Inhibitors - Roche) and left on ice for 8 min ...
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bioRxiv - Biophysics 2022Quote: ... culture medium was collected and cells were gently washed twice with 5 ml cold PBS and lysed in 0.5 ml of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Biochemistry 2022Quote: ... cells were harvested and solubilized in the lysis buffer (20 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol) plus 1% LMNG and Protease Inhibitor Cocktail (Roche) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The bacterial pellet was resuspended in lysis buffer (7.5 mL PBS supplemented with 1% Triton, 10 μg/mL DNaseI, EDTA-free Protease Inhibitor Cocktail (Roche)) and was incubated for 1 h on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended with 20 mL lysis buffer (50 mM NaH2PO4, 0.5 M NaCl, pH 8) and 1 cOmplete Mini Protease Inhibitor Cocktail tablet (Roche) per 1 L of expression ...
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bioRxiv - Cell Biology 2022Quote: H3K4me3-immunoprecipitated genomic DNA was diluted 1:100 and Chip-qPCR performed with a Light Cycler 480II machine (Roche) using technical duplicates and ChIP-qPCR signals were calculated as % of input ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were incubated in a blocking solution containing maleic acid buffer (pH 7.5) and 1 % blocking reagent (Roche). Subsequently ...
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bioRxiv - Genomics 2022Quote: NGS data from German field samples was analysed by mapping all reads against the ASFV Germany 2020/1 (LR899193) [18] genome sequence as reference using Newbler 3.0 (Roche) with default parameters including adapter and quality trimming ...
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bioRxiv - Genetics 2022Quote: ... resuspended in lysis buffer (25 mM Tris-HCl at pH 7.4, 150 mM NaCl, 10% glycerol, 1% NP-40, Roche Complete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Developmental Biology 2022Quote: ... The probe was mixed in hybridization buffer (0.1 g/ml dextran sulfate [Sigma D8906-50G], 1 mg/ml Escherichia coli tRNA [ROCHE 10109541001] ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche) and 1 µl of RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: 7-day-old Q28 and Q69 seedlings were lysed in lysis buffer (1% Triton X-100, 50 mM Tris–HCl pH 8.0) supplemented with protease inhibitor cocktail (Roche) and 25 mM N-ethylmaleimide ...
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bioRxiv - Plant Biology 2022Quote: ... and the interaction between AtRsmD and the selected proteins was determined by immunoblot analysis with a mouse anti-GFP monoclonal antibody (1:5,000; Roche) and a mouse anti-Myc monoclonal antibody (1:10,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 60 mM KCl, 15 mM NaCl, 1 mM CaCl2, 0.9% Triton X-100, and 13 protease inhibitor cocktail [Roche]). After homogenization ...
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bioRxiv - Molecular Biology 2022Quote: ... cell pellets were lysed in whole-cell extraction buffer (150 mM NaCl, 50 mM Tris pH 7.5, 1% NP-40) supplemented with proteinase and phosphatase inhibitors (Roche). The lysates were cleared by centrifugation at 14,000 rpm for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were incubated overnight at 4°C with monoclonal mouse anti-GFP antibody (Roche; Basel, CH; dilution 1:500) or monoclonal rat anti-HA antibody (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Dissected cortexes were collected in 24 well-plate and cut into small pieces to be digested in 1.5 ml of digestion buffer containing 1 mg/ml of collagenase/dispase cocktail (Roche) and 0.5 mg/ml of DNase I ...
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bioRxiv - Plant Biology 2023Quote: ... in 100μl GTEN-buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl,1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... A GTEN-based buffer (10% Glycerol, 25mM TRIS pH 8, 0.5mM EDTA, 150 mM NaCl, 1 x protease inhibitors (Roche), 5mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were collected as the cytoplasmic fraction and pellets were resuspended in 200 µL cold nuclear lysis buffer (20 mM HEPES pH 7.9, 100 mM NaCl, 1 mM DTT, Roche protease inhibitor cOmplete tablets EDTA free ...
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bioRxiv - Plant Biology 2022Quote: ... and vortexed vigorously in extraction buffer 1 (50 mM Tris–HCl pH 7.5, 150 mM NaCl, Complete EDTA-free Protease Inhibitor cocktail (Roche), PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of OsHV-1 and total bacteria were performed using quantitative PCR (qPCR, Roche LightCycler 480 Real-Time thermocycler) with the following program ...
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bioRxiv - Cell Biology 2023Quote: ... cardiomyocytes were dissociated using DPBS for 20 min at 37 °C followed by 1:200 Liberase TH (Roche, 5401151001) diluted in DPBS for 20 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibodies were incubated for 1 h at 37°C while nuclei were counterstained with DAPI (Roche) at RT for 5 min before mounting the glass coverslips using DAKO mounting medium (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... washed and resuspended in lysis buffer (50 mM Tris pH 7.5; 150 mM NaCl; 1 mM MgCl2; 10% Glycerol; Complete protease inhibitor (Roche); 1 mM PMSF or 2 mM AEBSF-hydrochloride (BioChemica APA1421.0001) ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting cDNA was analysed by qRT-PCR on a LightCycler 480 using SYBR Green 1 Master Mix (Roche). Relative fold change in expression was determined by the ddCt method ...
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bioRxiv - Cancer Biology 2023Quote: ... Each suspension was then supplemented with 0.1 mL of RPMI containing 1.25 mg mL−1 Liberase TM (Roche, 5401119001) and 20 mg mL−1 DNase I (Roche ...
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bioRxiv - Genetics 2023Quote: Mouse tissue was homogenized in 400 μl of RIPA homogenizing buffer (50-mM Tris HCl pH 7.4, 150-nM NaCl, 1-mM EDTA, supplemented with protease-inhibitor cocktails (Roche)) and lysed with a MagNA Lyser ...
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bioRxiv - Genomics 2023Quote: Fresh pinch biopsies were washed twice in DPBS before being coarsely chopped with a scalpel and transferred into 0.5 mL DPBS solution containing 0.1 WU ml−1 Liberase TH (Roche, 5401151001) and 0.5 U ml−1 RQ1 DNase I (Promega ...
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bioRxiv - Immunology 2023Quote: 50,000 sorted cells were washed with PBS and resuspended in Buffer 1 (1X eBioscience Perm/Wash Buffer, 1X Roche cOmplete EDTA-free Protease Inhibitor ...
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bioRxiv - Microbiology 2023Quote: ... released by trypsin and incubated in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then released by trypsin and lysed in ice-cold lysis buffer (Tris-HCl pH 8.0, 50mM, NaCl 150mM, EDTA 1mM, NP40 1%, cOmplete Mini Protease Inhibitor Cocktail (Roche) and phosphatase inhibitor PhosSTOP (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... Western blotting was conducted as described above and visualized with anti HA-HRP antibody (Roche 39 #12013819001; 1:2,000).
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM KCl, 0.5% [v/v] NP-40, 1 mM EDTA, EDTA-free cOmplete mini protease inhibitor [Roche, 1186170001], PhosSTOP [Roche, PHOSS-RO] ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated overnight at 4°C: rabbit anti-HA high affinity at 1:500 (Roche Diagnostics #ROAHAHA), mouse anti-FLAG M2 at 1:500 (Sigma-Aldrich #F1804) ...
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bioRxiv - Immunology 2023Quote: ... enzymatic digestion of tissue pieces was done with collagenase D (1 mg/ml) and DNAseI (0.1 mg/ml) (both from Roche) in HBSS/10%FBS for 45 min (37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in lysis buffer (300 mM KCl, 1 mM MgCl2, 0.1 mM ATP, 1X Roche Protease/PPase inhibitor ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in immunoprecipitation (IP) buffer (1× PBS, 0.1% Triton X-100, and cOmplete EDTA-free protease inhibitor cocktail [Roche]) and then disrupted by three pulses of sonication for 5 s ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of 180 mM of adenosine triphosphate (ATP) and 0.5 U of Glucose-6-phosphate dehydrogenase(G6PDH) (Roche). After the absorbance at 340 nm was recorded for 20 min to determine a baseline ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were taken up in sonication buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA, 1% SDS, cOmplete protease inhibitor by Roche) at a ratio of 10 million cells in 150 µL (120 uL for ChIP-seq) ...
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bioRxiv - Molecular Biology 2023Quote: ... isolated cell wall proteins were probed with anti-HA peroxidase-conjugated antibody (1:1,250 dilution; 11667475001, Roche, Basel, Switzerland).
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bioRxiv - Plant Biology 2023Quote: ... pH 7.0, 1 mM EDTA, 10 mM DTT, 0.05% [v/v] Triton X-100 and 1x Protease Inhibitor Cocktail [Roche]). Crude leaf extracts were incubated for 2 h at 4 °C on a turning wheel and centrifuged at 16.900 g for 5 min at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... 150 mM NaCl, 1 mM EDTA, 0.2% IGEPAL® CA-630, 0.5 mM DTT, 1X protease inhibitor cocktail [Roche]). Crude extracts were incubated on ice for 10 minutes and centrifuged for 15 minutes twice at 14.000 g ...
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bioRxiv - Genomics 2024Quote: ... Isolated nuclei were then lysed with lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor complete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...