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Citations for Roche :
6101 - 6150 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The cells were then rinsed with PBS and lysed at 4°C in Buffer A (1% Triton X-100, 0.5% NaDOC and 1X cOmplete Protease Inhibitor Cocktail (Roche) in PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... cell fusion was performed by incubating the dish with 1 ml of prewarmed polyethylene glycol 1500 (PEG 1500, Roche) for 1 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: Whole cell lysates were collected using RIPA lysis buffer (Beyotime Biotechnology, Jiangsu, China) containing 1% protease inhibitor (Roche, 11697498001) and 1% phosphatase inhibitor (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... The eluted proteins were separated on 8% SDS-PAGE gel (37.5:1 acrylamide:bis-acrylamide) and probed with anti-HA (Roche, 3F10), anti-GFP (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: ... Washed three times for 30 minutes each in TNTT at 4 ℃ to remove methanol.Blocked with 1% blocking reagents (Roche) in TNTT buffer for 2 hours at 4 ℃ to prevent no specific binding ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were lysed in SDS based lysis buffer (50 mM Tris Ph7.4, 150 mM NaCl, 1% SDS, 1mM EDTA and 50mM NaF) containing protease inhibitors (Roche) and sonicated ...
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bioRxiv - Biochemistry 2024Quote: Cells were lysed in native lysis buffer (10% glycerol, 25 mM NaCl, 20 mM HEPES pH 7.0, 1% DDM) supplemented with protease inhibitor tablet (Roche), and solubilized by rotating 30 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with primary antibody for 2.5 h (GFP, Abcam, Cambridge, UK: rabbit, 1:10000; HA (Roche), rat ...
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bioRxiv - Cell Biology 2024Quote: ... The bacterial pellet was then resuspended in ice-cold lysis buffer composed of 1x PBS (pH 7.4) and 1 tablet of protease inhibitor (Roche) per 10 ml ...
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bioRxiv - Neuroscience 2024Quote: ... 15 mM EDTA and 1% Nonidet P-40) supplemented with cOmpleteTM protease inhibitor and PhosSTOPTM phosphatase inhibitor cocktails (Roche). Lysates were subsequently subjected to GFP trapping ...
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bioRxiv - Molecular Biology 2024Quote: ... was transferred to a new tube and the pellet was resuspended with 300 µl membrane lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40) supplemented with protease inhibitors (Roche). After 20 min of incubation the samples were centrifuged again at 100’000 for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The powdered tissue was then mixed with 1 ml of lysis buffer per gram of tissue (150 mM NaCl, 0.1% Triton X, and 50 mM TRIS HCl-pH 8, supplemented with 13:1 of Complete Mini, Roche).
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed in HBSS and then dissociated using 1 mg/mL collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... were pelleted and resuspended in NP-40 lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-Cl pH 8.0) containing cOmplete protease inhibitor cocktail (Roche) and PhosSTOP phosphatase inhibitor cocktail (Roche) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1.5 mM MgCl2, 1 mM DTT, 0.1% Triton X-100 and protease inhibitors: cOmplete Mini, EDTA-free, Roche, #4693159001). Lysis was performed by sonication (10-20 cycles of 30 seconds ON and 30 seconds OFF ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 µg of GST-F-box beads were washed 3 times with pulldown wash buffer (25 mM Tris HCl pH 7.5, 150 mM NaCl, 1.5 mM MgCl2, 1 mM DTT and protease inhibitors: cOmplete Mini, EDTA-free, Roche, #4693159001). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... 1 µg of RNA was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). The PCR mix contained 10 ng cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were resuspended in 2 mL of ice-cold lysis buffer (50 mM Tris pH 7.6, 150 mM NaCl, 1% Triton X-100, protease inhibitors [Roche]) and rotated at 4°C for 20 mins ...
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bioRxiv - Cell Biology 2024Quote: ... The cell pellet was resuspended in Lysis buffer (20mM Tris-HCl pH 7.5, 150mM NaCl, 1% SDS, 1mM EDTA) containing protease inhibitor cocktail (Roche). The lysate was clarified by centrifugation (17,000xg for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0 and 1 %) complemented with complete protease inhibitor cocktail (PIC) (Complete EDTA-free Proteinase inhibitor cocktail tablets (11836170001, Roche)) ...
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bioRxiv - Microbiology 2024Quote: All the samples were lysed with high-salt buffer (50 mM Tris pH 7.5, 300 mM NaCl, 1% Triton-x-100, protease inhibitors (11836170001, Roche), and Pierce Phosphatase inhibitor cocktail (A32957 ...
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bioRxiv - Microbiology 2024Quote: ... Proteases were inactivated by the addition of 1 mM PMSF and 10 ul 1x complete protease inhibitor cocktail (Roche).
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bioRxiv - Microbiology 2024Quote: ... resuspended in 4 ml of a buffer (HEPES 10 mM pH 7.4, 1 mM EDTA, EDTA-free protease inhibitor cocktail from a tablet (Roche)) and lysed by 3 passages through a cell disrupter at 2.4 kbar ...
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bioRxiv - Immunology 2024Quote: ... Tissues were then incubated for 1.5 hours at 37°C in an orbital shaker (200 rpm) with 1 ml of digestion media containing 0.25 mg/ml Liberase TL (Roche) and 0.25 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... brachial and mesenteric lymph nodes from 3 mice/genotype were pooled and cut into pieces and digested with the 1 ml of enzyme mix containing 0.8 mg/ml Dispase (Roche), 0.2 mg/ml Collagenase P (Roche ...
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bioRxiv - Microbiology 2024Quote: ... Cells were resuspended in cold extraction buffer (50 mM Tris-HCl pH 7.5 containing 1 mM EDTA, 4 mM DTT, cOmplete Protease Inhibitor Cocktail (Roche), and 1 mM PSMF ...
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bioRxiv - Plant Biology 2024Quote: ... Plant tissues were ground in liquid nitrogen and the powder was suspended in lysis buffer (50 mM Tris-HCl at pH 8.0, 150 mM NaCl, 1 mM phenylmethanesulfonyl fluoride, 10 mM iodoacetamide, and 1X Roche cOmplete mini protease inhibitor cocktail) ...
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bioRxiv - Neuroscience 2024Quote: ... 1% NP-40 (vol/vol) supplemented with protease inhibitor cocktail (cOmplete EDTA-free Protease Inhibitor Cocktail, Roche, Cat# 05892791001) for 20 minutes at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... cells were lysed in IPH buffer (20mM Tris-HCl pH7.5, 150mM NaCl, 2mM EDTA, 1% NP-40) supplemented with protease inhibitor cocktail (Roche, cOmpleteTM EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended with 1 ml of single nuclei wash buffer containing 0.2 U/µl RNase inhibitor (3335402001, Roche) and transferred to a microcentrifuge tube ...
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bioRxiv - Biochemistry 2024Quote: His-tagged 53BP1 TTD was purified by resuspending thawed cell pellets in 30ml of lysis buffer (50mM sodium phosphate pH 7.2, 50mM NaCl, 30mM imidazole, 1 x EDTA free protease inhibitor cocktail (Roche) per litre of culture) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNAse from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Lysates were incubated with Ni-NTA agarose (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 1µg of DNase from bovine pancreas and 1 dissolved tablet of cOmplete EDTA-free Protease Inhibitor Cocktail (Roche). Pellets were then homogenized with a Biospec Tissue Tearor (Dremel) ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using NP-40 lysis buffer (100 mM Tris pH 8.0, 1% NP-40, 150mM NaCl) containing protease inhibitor cocktail (Roche) for 20 min with shaking on ice ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were then harvested by centrifugation and resuspended in buffer A (20 mM Tris-HCl, 1 mM EDTA, pH 8.0) including a protease inhibitor tablet (EDTA-free, Roche). Cells were lysed by sonication on ice before cell debris was removed by centrifuged (18,000 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellets were resuspended in 150 μl chilled lysis buffer (1% SDS, 10mM EDTA, 50 mM Tris-HCl pH8.1, 1x protease inhibitor cocktail, 1x PhosSTOP phosphatase inhibitors (Roche), 5 mM sodium butyrate (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cell pellets were resuspended in 1/10 volume of lysis buffer (0.2 M Tris-HCl, pH 8, 10 mg/mL lysozyme, and 1× EDTA-free protease inhibitors; [Roche]), incubated for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and supplemented with 850 μl 1% Triton X IP dilution buffer (1% Triton X, 20mM Tris-HCl pH8.1, 150mM NaCl, 2mM EDTA, 1x protease inhibitor cocktail (Roche), 1x PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... 12 mM MgCl2 and 150 mM KCl in RNAse free water; 0.5 mM DTT, 1 µL/mL Protease Inhibitors Cocktail [Roche] ...
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bioRxiv - Microbiology 2024Quote: ... and 10% glycerol) supplemented with the complete EDTA-free protease inhibitor cocktail (1 tablet/50 ml of lysate; Roche), frozen in liquid nitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Cells were lysed in DDM lysis buffer (50 mM Tris-HCl, pH 7.4; 150 mM NaCl; 1 mM EDTA; 50 mM NaF; 1mM sodium vanadate; 1x Roche Complete Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... Dicer was detected using anti-HA 3F10 monoclonal primary antibody (High Affinity rat IgG1, Roche #11867431001; dilution 1:500), anti-HA rabbit primary antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... 500 μg cell lysate was diluted to 500 μL in lysis buffer supplemented with 1 mM DTT and 25 μg poly(dI:dC) (Roche) and precleared by incubating with 50 μL streptavidin beads for 1 hour at 4°C on rotating wheel ...
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bioRxiv - Neuroscience 2024Quote: ... Protein extraction buffer (150 mM NaCl, 1% Triton x-100, 50 mM TrisHCl pH 7.4 with protease inhibitor (11836170001 Roche)) ...
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bioRxiv - Physiology 2024Quote: ... cells were lysed in IP buffer (10 mM Tris pH 8, 0.4% NP40, 300 mM NaCl, 10% glycerol, 1 mM DTT, antiprotease (Roche), anti-phosphatase (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Aggregate fractionation buffer (100 mM HEPES pH 7.4, 1% Triton X-100, 300 mM NaCl) supplemented with Protease Inhibitor Cocktail (Roche) and 25 mM N-ethylmaleimide was added at 3 volumes per unit mass of dried seedling weight ...
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bioRxiv - Neuroscience 2024Quote: Each brain hemisphere was added to a 7 mL Precellys tube with pre-loaded zirconium oxide beads (Precellys, Bertin, USA) and homogenized in ice cold 0.02% CHAPS in 1x PBS with protease inhibitors (1 Roche cOmplete tablet 4693159001 ...
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bioRxiv - Neuroscience 2024Quote: ... protease inhibitor (1 tablet per 10 ml solution; cOmplete ULTRA Tablets-Mini, F. Hoffmann-La Roche AG, Basel, Switzerland) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells are then washed twice in cold 1% BSA solution containing 0.5X EDTA-free Protease inhibitor cocktail (PIC, Roche), and once in cold PBS ...
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bioRxiv - Genetics 2024Quote: ... The amplification PCRs (Primers 1-6 Table S7) were done with Kapa HiFi HS Ready Mix (Kapa Biosystems 07983034001), using 20 ng input template and 50 ul final volume except for the High GC bin in which 20 ul of KAPA2G GC Buffer (ROCHE KK5516 ...