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Citations for Roche :
6301 - 6350 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Total RNA from 4 replicates of BCBL-1 WT clone B4 and 57KO clone #6 cells were isolated by TriPure Reagent (Roche) 24 h after induction with VA ...
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bioRxiv - Plant Biology 2022Quote: ... The ELVd probe was obtained via in vitro transcription of a linearized plasmid for 1 h at 37°C with 20 U of T3 bacteriophage RNA polymerase (Roche) in 40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed on ice for 15 minutes with 100 µl lysis buffer (50 mM HEPES, 150 mM NaCl, 1% TritonX100) supplemented with protease and phosphatase inhibitor cocktail (Roche). 10% of the soluble fraction of the lysate was boiled with 4X sample buffer ...
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Neurotransmission and neuromodulation systems in the learning and memory network of Octopus vulgarisbioRxiv - Neuroscience 2021Quote: Immunological detection was performed using antidigoxygenin-AP Fab antibody fragments at a dilution of 1:2000 (Roche Diagnostics, Mannheim, Germany). After probe hybridization ...
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bioRxiv - Molecular Biology 2020Quote: ... 4×15cm plates of confluent WT F1 2-1 MS2129 female ESCs were lysed by pipetting in 3mL of lysis buffer (10M Tris-HCl pH8, 137mM NaCl, 1% NP40, 2mM EDTA) supplemented with 1x Complete EDTA-free Protease Inhibitors (Roche) and incubated for 1h on ice with or without RNase (10μg/mL RNase A ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100, pH 7.4, containing phosphatase inhibitors (10 mM NaF, 1 mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and were homogenised in TBS pH 7.4, 1% NP-40 containing phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche). Protein concentration was adjusted to 10 mg/ml by the Bradford method (Pierce ...
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bioRxiv - Molecular Biology 2020Quote: ... Sequencing libraries were constructed from 1 ng of immunoprecipitated and input DNA using the KAPA Hyper Prep kit (KAPA Biosystems) and NEXTflex ChIP-seq barcodes (Bio Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and the soluble fraction was incubated for 1 h at 4°C with beads crosslinked to mouse anti-GFP antibody (Roche). Resulting Immunoprecipitates were washed three times with lysis buffer and protein eluted with RapiGest surfactant (Waters ...
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bioRxiv - Immunology 2022Quote: ... In short lungs were minced with scissors and incubated in a digestion cocktail containing 1 mg/mL collagenase A (Roche) and 30 μg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in 500 μl lysis buffer (10 mM Tris HCl, 10 mM NaCl, 0.3% NP40, 1× Roche Complete) and kept on ice ...
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bioRxiv - Immunology 2021Quote: ... Colon tissue samples devoid of intra-epithelial cells as well as lung and skin samples were enzymatically digested with 1 mg/ml Collagenase A from Clostridium histolyticum (Roche) and 1 unit/ml DNaseI (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... PrPSc levels were determined through digestion of 20 μg of COCS homogenates with 25 μg mL-1 of proteinase K (PK, Roche) at a final volume of 20 μL in PBS for 30 minutes at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sense and antisense digoxigenin-labeled RNA probes were prepared from a BglII-digest of the pMPX2-1 mouse Pax6 cDNA clone (25) using a DIG RNA labeling kit (Roche). Hybridization and stringent posthybridization wash steps were performed at 65°C.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Paw tissues were minced and digested in 1 mg/ml collagenase A / 2.4 U/ml dispase II (Roche Applied Sciences) in HEPES buffered saline (Sigma ...
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bioRxiv - Biochemistry 2020Quote: Cells were resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Triton X100, 0.3 mM TCEP, 1X Roche cOmplete tablet) and lysed by three cycles of rapid freeze-thaw ...
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bioRxiv - Developmental Biology 2020Quote: ... a final probe concentration of 0.1 ng/μL was used and the probe was detected using an alkaline phosphatase conjugated anti-digoxigenin antibody (1:1500; Roche) and NBT/BCIP (Roche ...
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bioRxiv - Immunology 2020Quote: ... single cell suspensions were prepared from the tumors after mechanical disruption and enzymatic digestion with 1 mg/mL Type IV Collagenase (Roche). Live tumor infiltrating CD8+ T cells (CD3+ CD8+ CD4− ...
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bioRxiv - Biochemistry 2021Quote: ... 150 mM sodium chloride (NaCl), 20% glycerol, and 1 mM Dithiothreitol (DTT, Scientific) supplemented with one Complete EDTA free protease inhibitor tablet (Roche) per 50 ml lysis buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated with 0.5% blocking reagent in PBT before an overnight incubation in 1:2000 anti-dig AP antibody (Roche, 11093274910) in 0.5% blocking reagent (Roche ...
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Elevated Hoxb5b expands vagal neural crest pool and blocks enteric neuronal development in zebrafishbioRxiv - Developmental Biology 2021Quote: ... Probed embryos were blocked for 1-2 hours at ambient temperature in 5% Goat sera in PBST before detection overnight (∼16 hours) at 4°C using an anti-Digoxigenin-Fab fragments conjugated to Alkaline Phosphatase enzymes (1:1000 dilution, Roche) in 5% Goat Sera in PBST ...
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bioRxiv - Genomics 2020Quote: ... before incubation at 37°C with 1 µg of double stranded DNA (atr amplicon, Jones et al., 2003) in the presence of 1x M buffer (Roche). Filtrated supernatants (10 µl ...
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bioRxiv - Immunology 2021Quote: Strand-specific ribosomal RNA depleted RNA-seq libraries were prepared from 1 µg of total RNA using the KAPA Stranded RNA-seq Kit with Ribo-Erase (Roche). Samples were prepared in at least triplicate for analysis ...
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bioRxiv - Microbiology 2021Quote: ... following which the pellet was re-suspended in 50 ml of buffer A (PBS with 10 mM DTT and 1 x protease Inhibitor Cocktail (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated with anti-digoxigenin conjugated with alkaline phosphatase (1:2500; sheep anti-DIG-AP Fab fragments, Roche Diagnostics ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the detection of the Dig-labelled riboprobe with an anti-DIG Fab fragment conjugated with alkaline phosphatase (1:750; Roche). The colometric reaction was performed using mixture of 5-Bromo-4-chloro-3-indolyl phosphate (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Transfected HEK29T cells and COS-7 cells were washed and lysed with 1% Triton X-100 in PBS with protease inhibitor cocktail (Roche) at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... cell lysates were retrieved in 1% sodium deoxycholate (SDC) in phosphate buffered saline solution with Protease Inhibitor Cocktail (cOmplete, Roche) buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM imidazole and 10 % glycerol supplemented with EDTA-free protease inhibitor tablets (Roche; 1 tablet per 100 ml buffer) and lysozyme (1 mg/ml) ...
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bioRxiv - Biochemistry 2020Quote: ... Membrane pellets were resuspended to 40 mg ml-1 in ice-cold buffer A supplemented with a protease inhibitor tablet (Roche) and 100 μM PMSF ...
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bioRxiv - Neuroscience 2020Quote: ... All qPCR reactions were performed in 10 μl volume in triplicates with 1× LightCycler 480 SYBR Green I Master kit (Roche) and primers listed in Supplementary Table 1 on LightCycler® 480 PCR instrument II (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) analyses of cDNA (diluted at 1/10) were performed in triplicate with a LightCycler 480 II (Roche) using the SYBR Green I Master mix (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using RIPA buffer (50 mMTris–HCl, 10 mM MgCl2, 20% Glycerol and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche). Protein concentration was determined by BCA assay (Pierce ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... kidney (human primary renal proximal tubule epithelial cells, RPTEC) and liver (human hepatocellular carcinoma, HepG2) cells were assessed using a standard WST-1 (Roche) cell viability assay ...
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bioRxiv - Microbiology 2021Quote: ... Two days after induction, cells were lysed with lysis buffer (1x PBS, 1% NP-40, 1x protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2021Quote: ... A 1/10 dilution of cDNA was used for qRT-PCR analysis using the FastStart Universal SYBR Green Master Mix (Roche). Sequences of the primers used are listed in the reagents or resource table.
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bioRxiv - Cell Biology 2021Quote: Cells were lysed with 0.1% Triton in PBS- (phosphate-buffered saline without CaCl2 or MgCl2) supplemented with EDTA-free protein inhibitor cocktail (complete, Roche) on ice ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were resuspended in ice-cold NP-40 Buffer (150mM NaCl, 1% NP-40, 50mM Tris pH8) containing EDTA free protease inhibitors (complete cocktail, Roche) and lysed using a 1ml tissue homogenizer (Fisher) ...
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bioRxiv - Plant Biology 2021Quote: ... and the presence of SnASFF1-HT was confirmed by immunoblot using the BMG–His-1 monoclonal antibody (Roche, Basel, Switzerland). Purified SnASFF1 proteins were stored in 100 mM sodium acetate (pH 4.5 ...
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bioRxiv - Neuroscience 2021Quote: ... embryonic cortices were isolated and stored either in lysis buffer (48% Urea, 14 mM Tris pH 7,5, 8,7% Glycerol, 1% SDS, protease inhibitor cocktail (Roche, Basel, CH)) or RNAlater (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and digested in digest buffer (RPMI supplemented with 10% FBS, 1% penicillin/streptomycin [Invitrogen], 1mg/mL collagenase D [Sigma], 1mg/mL dispase [Sigma], 40 μg/mL DNAse [Roche]) at 37° C for 30 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was generated from 1 μg total RNA with qScript cDNA SuperMix (Quantabio) and analyzed on a LightCycler 480 II apparatus (Roche) with the SYBR Green I Master mix (Roche ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid NL4.3 and various mutants were transfected into JLTRG-R5 cells with 1 μg of viral DNA using Xtreme-GENE HP (Roche) Transfection reagent ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Neuroscience 2020Quote: ... Lysis was performed using mechanical scraping in lysis buffer (1% IGEPAL-CA630 and 1mMPMSF with Roche complete protease inhibitor tablet) and subsequently spun down at 500xg for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples were homogenized in pre-chilled lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, protease inhibitor cocktail (Roche 04693132001) and phosphatase inhibitor (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting powder was resuspended in 50 ml TAP Lysis buffer plus 1 mM PMSF and 1X Complete EDTA-free protease inhibitor cocktail (Roche). The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl ...
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bioRxiv - Microbiology 2021Quote: ... The column was washed with 200 mL of the equilibration buffer supplemented with 1 mM PMSF and EDTA-free protease inhibitor (Roche). SolPacL1 and SolPacL1Δ3 were then eluted with 25 mL of the equilibration buffer containing 1 mM PMSF and 250 mM imidazole.
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bioRxiv - Plant Biology 2021Quote: ... The construct was used to transform wild type plants following the standard floral dip method (Clough and Bent, 1998) and selected with 50 mg L-1 Hygromycin (Roche). A total of 60 T1 plants were individually genotyped by PCR and sequencing ...