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Citations for Roche :
5901 - 5950 of 8217 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and then resuspended in 3 mL of lysis buffer (160 nM NaCl, 20 mM, Tris-HCl pH7.5, 1 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Biophysics 2020Quote: ... the pool was loaded in 0.5 mL GST resin mixed with 1 mL of cOmplete His-tag purification resin (Roche) stacked in a 12 mL polyprep column ...
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bioRxiv - Cell Biology 2021Quote: ... 200 μg of mitochondria were lysed in 20 μL of lysis buffer (50 mM KPi pH 7.4, 150 mM KCl, 1× Complete Protease Inhibitor cocktail (Roche), 1 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... human iPSC-derived neuronal cultures and N2a cells were collected in Lysis Buffer (50 mm Tris-Base, 150 mm NaCl, 1% Triton X-100, 0.5% deoxycholic acid) with protease inhibitor (Roche) and phosphatase inhibitor cocktails II and III (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... protein extracts were obtained by lysing cells in lysis buffer (50 mM Tris-pH: 7.5, 150 mM NaCl, 1% Triton-X100, 1x Complete Protease Inhibitor Cocktail (Roche)) ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... The slides were incubated overnight with a 1:5,000 dilution of alkaline phosphatase-conjugated anti-DIG antibody (Roche Diagnostics). After the slides had been washed in in a solution of 0.1 M Tris (pH 7.5 ...
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bioRxiv - Physiology 2021Quote: ... Reactions were performed in a 6 μl volume of 1 x Kapa Probe Fast Mastermix (Kapa Biosystems, Amsterdam, Netherlands). TaqMan assays (FAM labelled ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in KPBS and incubated with rabbit anti-FG antibody (1:5000; Chemicon International, Calif, USA) in 0.5% Blocking Reagent (Roche) in KPBS for 16 h at 4 ° C ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... The membrane was blotted overnight at 4°C with rat antibody against the HA tag (Roche, 1/500 dilution), TRAF2 and TRAF6 rabbit antibodies (Suppl ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl) and resuspended in 1 ml Tris-Buffered saline supplemented with protease inhibitor (cOmplete Protease Inhibitor, Roche). Bacterial lysis was done by enzymatic cell wall degradation (30 min at 37°C with 10K/ml mutanolysine ...
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bioRxiv - Genetics 2022Quote: Cell line lysates were made in NP40 lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP40) supplemented with protease inhibitors (Roche). Next ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfer membranes were then incubated with a 1:10,000 dilution of primary mouse anti-6xHis antibody (Roche, cat. #11922416001) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Neurons were harvested on DIV14 and lysed by addition of lysis buffer (50 mM Tris pH 8.0 and 1% SDS containing cOmplete Mini Protease Inhibitor (Roche)) and boiling at 95°C for 5 min ...
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bioRxiv - Cell Biology 2022Quote: HEK293T lysates were prepared using IP lysis buffer (150 mM NaCl, 25mM Tris-HCl pH=7.5, 1% Triton-X and protease cocktail inhibitor from Roche). Rabbit reticulocyte lysates were obtained from Promega ...
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bioRxiv - Neuroscience 2022Quote: ... Digoxigenin probes were made by standard protocols and were detected using the anti-DIG POD antibody (Roche, 1:1000) and stained using Cy3-tyramide substrate (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: ... 100 ng cDNA from each sample was subjected to quantitative real-time PCR (qPCR) following the manufacturer’s instructions of PerfectStart Green qPCR SuperMix (TransGen Biotech, #1) on LightCycle480 Instrument II (Roche). Relative mRNA expression was normalised to RPLP0 using standard statistics method of 2-ΔΔCT.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... pH 8.0) augmented with protease and phosphatase inhibitors (1:100 HALT PI cocktail, Thermofisher Sci Cat# 78440; or Roche cOmplete ULTRA [Cat# 05892970001] plus phosStop [Cat# 4906845001] tablets) ...
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bioRxiv - Cell Biology 2022Quote: ... 10mM Tris-HCl pH 8, 1 mM DTT, 0.2% NP-40, 1x protease inhibitor [Roche], 1x phosphatase inhibitor [Roche]), sonicated and centrifugated for 10 minutes at 14,000 RPM at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed 3x with PBS and then lysed in 1 ml TX-100 lysis buffer (1% Triton X-100, 150mM NaCl, 50 mM Tris-HCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Cell lysates were cleared by centrifugation at 10,000 × g for 10 mins at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Released nuclei were washed (10 mM Tris-HCl pH 8, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 1x PIC (Roche)) for 5 minutes at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 1 µg RNA was used to synthesize cDNA using a first-stand cDNA synthesis kit (Roche Diagnostics, Switzerland). The cDNA was normalized to 100 ng µl−1 and used for PCR analysis in a 10 µl reaction with gene-specific primers (5‘-CGTGGGATACCCTTACATGG and 5‘-CCCGATTATCTTCCACCAGA for ZmOMT1 ...
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bioRxiv - Genomics 2020Quote: ... Isolated nuclei were then lysed with lysis buffer (50mM Tris-HCl, pH 8.1, 10 mM EDTA, pH 8, 1% SDS, 1X protease inhibitor cOmplete EDTA-free cocktail, Roche). Nuclei were sonicated using a Bioruptor ultrasonic cell disruptor (Diagenode ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted at 500 × g for 10 min and lysed in TNTE buffer (1:10 wt:vol) supplemented with protease inhibitor cocktail (Roche) by homogenization using motorized Teflon-glass homogenizer (Thomas Scientific ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... 150mM NaCl, 10% glycerol, 5mM MgCl2, 0.1% Tween20, 15mM EGTA, 10μM MG132, and 1× cOmplete™ Protease Inhibitor Cocktail [Roche]) and incubated for 30 min at 8 rpm in the cold room ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% Triton X-100) supplemented with Complete® protease inhibitor cocktail (Roche, 1 pastel per 10 mL lysis buffer) for 5 min ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... 1% Triton X-100 plus protease and phosphatase inhibitor cocktail tablets (Complete mini, EDTA-free, Phos-STOP from Roche). Insoluble cell material was rejected by centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1× protease inhibitors complete mini (Roche)) ...
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bioRxiv - Physiology 2021Quote: ... we collected feces of 90 adults that had fed on Arabidopsis wild type leaves for one day in a total volume of 1 mL 20 mM MES buffer pH 6.5 containing protease inhibitors (cOmplete, EDTA-free, Roche). After homogenization with metal beads for 3 min at 25 Hz in a TissueLyzer II and centrifugation at 4 °C for 10 min at 16,000 × g ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.2ml of Buffer NUN2 (300mM NaCl, 7.5mM MgCl2, 1% NP-40, 1M Urea supplemented with complete EDTA free protease inhibitor (Roche) and PhoSTOP (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Pellets were resuspended in buffer (50mM Tris-HCl pH 7.0, 100 mM NaCl, and 1 tablet anti-protease cocktail (Roche) for 25 mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... 2003) except that 1 to 600 dilution of anti-DIG-HRP conjugated and anti-FLU-HRP conjugated antibodies (Roche 11207733910 and 11426346910 ...
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bioRxiv - Cancer Biology 2020Quote: ... were resuspended in 50 μl PBS following the addition of 50 μl 1% SDS in 100 mM Hepes/NaOH pH8.5 supplemented with protease inhibitor cocktail EDTA-free (Roche). Samples were heated to 95°C for 5 min ...
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bioRxiv - Neuroscience 2020Quote: ... The brains were transferred to a 1.5 ml Eppendorf tube containing 500 microliters 1 mg/ml Liberase DH (Roche, prepared according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2020Quote: Cultured cells were lysed in RIPA buffer containing protease inhibitor cocktail (1 tablet/15 mL) (Roche Diagnostics, Mannheim, Germany), sonicated and stored at −80°C until analysis ...
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bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
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bioRxiv - Genetics 2021Quote: ... 0.1% SDS) and resuspension of the nuclei in 0.9 mL Covaris shearing buffer (with 1 × protease inhibitors complete mini (Roche)) ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mM Tris HCl [pH 8.0], 10 mM MgCl2, 1 mM EGTA, and 1X protease-inhibitor cocktail [11697498001, Roche]), and incubated for 30 min at 4°C with gentle rocking ...
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bioRxiv - Biochemistry 2021Quote: ... The cell pellet was resuspended in 40 mL lysis buffer (50 mM NaH2PO4 pH 7.4, 300 mM NaCl, 1 mM PMSF and Complete protease inhibitors without EDTA (Roche)) and sonicated on ice ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl; 20 mM Tris-HCl [pH 7.5]; 1 mM EDTA, 10% glycerol; complete protease inhibitor cocktail [Roche]). Samples were mixed (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... 2011) or Tyramide Signal Amplification (TSA) after incubation with anti-digoxigenin-AP or anti-FITC-POD (Roche, 1:300) antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... and resuspended in SDS lysis buffer (50 mM Tris-HCl [pH 8.1], 1% SDS, 10 mM EDTA) with complete protease inhibitor cocktail (Roche). Cell extracts were sonicated with a Bioruptor TOS-UCW-310-EX (output ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C in B1 buffer with alkaline phosphatase-conjugated anti-DIG (1/2000; Roche 11633716001). After three washes in B1 buffer and one wash in B3 buffer (100 mM Tris–HCl pH 9 ...
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bioRxiv - Genomics 2021Quote: ... Samples were then incubated overnight at 4°C with a rabbit anti-DIG HRP-conjugate antibody (1:500, Roche). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Brain tissues were homogenized using mortar/pestle with liquid nitrogen and then lysed in ice-cold RIPA buffer (50mM Tris-HCL pH=7.4, 150mM NaCl, 1% Triton X-100, 0.1% SDS) (Beyotime, P0013B) freshly supplemented with protease inhibitor (Roche, 11836145001) and phosphatase inhibitor (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 animals per group were dissected in CMFB (CMF containing 0.5% BSA) and dissociated using 1:50 Liberase (2.5mg/mL, Roche 5401135001) in CMFB at 30°C with agitation at 300 rpm for 30 minutes on an Eppendorf ThermoMixer ...
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bioRxiv - Developmental Biology 2020Quote: Transfected HEK cells were lysed in cold immunoprecipitation buffer (1% NP-40, 50 mM Tris-HCl, pH 8.0, 150 mM NaCl) with protease inhibitors (Roche), and centrifuged for 10 minutes and 3,000 rpm at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with 1:2000 alkaline phosphatase-conjugated anti-DIG antibodies (Sigma/Roche, 11093274910). After washes and prior to development ...
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bioRxiv - Developmental Biology 2021Quote: ... 20% heat-inactivated lamb serum in Tris-buffered saline with 0.1% Tween-20 (TBST) and incubated with alkaline phosphate-conjugated anti-DIG antibody (1:2000, Roche) in blocking buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... for 2hr at room temperature and then incubated in blocking solution containing anti-Digoxigenin-AP antibody (1:2000; Roche) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 50 ng of nick translated probe per coverslip was combined with 12 μg of human Cot-1 DNA (Roche), 10 μg salmon sperm ssDNA (Sigma-Aldrich ...