Labshake search
Citations for Roche :
5501 - 5547 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the pocks positive for both BleCherry and GFP signals (pocks containing the recombinant green/red virus) were isolated for DNA extraction using High Pure Viral Nucleic Acid Kit (Roche Diagnostics GmbH, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... To correct ONT reads another library using the same HMW sample as above was constructed for shotgun sequencing with the Hyper Library construction kit from Kapa Biosystems (Roche, Basel, Switzerland) and sequencing was performed using an Illumina NovaSeq at the Roy J ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR primer for generating the probe was 5’-GGTTCTGTGATTGAGTGTTTGGATCTCCCTGCG-3’ with a single-end CY3 tag generated using the DIG RNA Labeling Kit (Roche Applied Science, Penzberg, Germany) based on the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Libraries for target enrichment of ∼3 Mb of Lupinus angustifolius genomic material were produced using the Roche Sequencing Solutions’ ‘SeqCap EZ – HyperPlus’ kit (Roche Sequencing Solutions, Pleasanton, CA) with 200 ng/L of input DNA.
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bioRxiv - Plant Biology 2023Quote: ... and purified based on the manufacturer’s instructions before sample barcoding with xGen Stubby Adapter and Unique Dual Index Primers (IDT) and library preparation using KAPA HyperPrep Kit (Kapa Biosystems-Roche, Wilmington, MA). The library was then sequenced by Illumina MiSeq nano PE150 at the Georgia Genomics and Bioinformatics Core ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections as previously described (46) (Iba-1 staining was conducted on a Ventana Benchmark using OptiView and UltraView detection kits provided by Roche (Roche Molecular Systems, Inc). Sections were deparaffinized in xylene and rehydrated through an ethanol series ending in distilled water ...
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bioRxiv - Physiology 2023Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems, Wilmington, Massachusetts, USA). For ovulated mature eggs ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription (RT) was performed from 2-3 µg of RNA with the Transcriptor First Strand cDNA Synthesis Kit (Roche Life Sciences, Basel, Switzerland) using random hexamer primers ...
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bioRxiv - Zoology 2023Quote: ... Paired-end cDNA libraries were constructed from 4 μg of total RNA using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Inc., Wilmington, USA) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: RT‒qPCR was conducted by using a KAPA SYBR Fast qPCR kit (Nippon Genetics) with a LightCycler 480 II system (Roche Applied Science, Penzberg, Germany). The PCR reactionwas conducted under the following conditions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and ligated to iTru adapters followed by limited-cycle PCR of iTru primers to add indexes and complete the library molecules using Kapa Library Preparation Kit reagents (Kapa Biosystems [Roche, Basel, Switzerland]). We sequenced the pooled libraries on an Illumina sequencer (Illumina ...
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bioRxiv - Genomics 2024Quote: ... RNA-seq libraries were prepared from 500 ng of total RNA using the KAPA Stranded RNA-seq kit with RiboErase (07962304001, Kapa Biosystems, Wilmington, MA, USA) for rRNA depletion and Illumina compatible indexes (NEXTflex RNA-seq Barcodes ...
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bioRxiv - Microbiology 2024Quote: ... hoffmanii kept in RNAlater buffer were transported to DZIF where total nucleic acids (RNA) were extracted using the MagNA Pure 96 DNA and Viral NA Large Volume Kit (Roche Diagnostics, Indianapolis, IN, USA) in a Magna Pure 96 Instrument (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2024Quote: ... We used identical input total RNA (50 ng to 500 ng) between control and samples for library preparation using the KAPA RNA HyperPrep Kit (KAPA Biosystems, Wilmington, MA, USA). Libraries were analyzed by Agilent 2100 bioanalyzer system for quality control ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the KAPA stranded mRNA-Seq Kit for the Illumina® Platform (KR0960-v.5.17; Kapa Biosystems, Wilmington, MA, USA), and sequencing were performed on an Illumina Novaseq 6000 instrument via NEO Bio (Shanghai ...
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bioRxiv - Plant Biology 2024Quote: ... These cDNA plasmids were used as templates and antisense RNA probes were synthesized using the DIG RNA Labeling kit (Roche Diagnostics GmbH, Mannheim, Germany).
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bioRxiv - Pharmacology and Toxicology 2024Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2024Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by RT-qPCR using a commercially available kit (KAPA Biosystems; Roche Basel, Switzerland) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer.
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bioRxiv - Immunology 2021Quote: ... Quality of libraries were determined via Agilent 2200 TapeStation using High Sensitivity D1000 tape and quantified using Kappa SYBR®Fast qPCR kit (KAPA Biosystems, Inc, Wilmington, MA). Approximately 60-80 million paired-end 150 np sequence reads were generated per sample using the Ilumina HiSeq 4000 sequencer.
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bioRxiv - Immunology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist.
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bioRxiv - Microbiology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems, Roche Holding AG, Basel, Switzerland). Sequencing libraries were loaded on a NextSeq500 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159) (Roche Diagnostics Corp. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: DNA was extracted from 1 mL of peripheral blood samples using a MagNA Pure LC DNA Isolation Kit in an automatic DNA extractor (MagNa Pure® System, Roche Applied Science, Indianapolis, Indiana). Thenceforth ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR was performed using the extracted DNA (100 ng) as template with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... and 2.55 nL of 72 Ad153-reverse-tag (1~72) primer [1 μM] made by KAPA HiFi HotStart PCR Kit (KK2500, Kapa Biosystems, Cape Town, South Africa). Finally ...
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bioRxiv - Plant Biology 2021Quote: ... Library quality was assessed using a Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Kapa Biosystems, Inc, Wilmington, MA, USA). RNA-seq experiments have been performed on an Illumina HiSeq3000 using a paired-end read length of 2×150 bp with the Illumina HiSeq3000 sequencing kits.
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bioRxiv - Microbiology 2020Quote: ... DNA isolation was performed with a MagNA Pure LC total Nucleic Acid Isolation kit in the MagNA Pure® system (Roche Diagnostics, Almere, the Netherlands). Samples were tested with a Chlamydiaceae PCR targeting the 23S rRNA and C ...
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bioRxiv - Microbiology 2021Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). All tissue slides were evaluated by a board-certified veterinary pathologist and a pathology scored was assigned based on the following observations ...
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bioRxiv - Developmental Biology 2020Quote: ... either SP6 or T7 RNA polymerase was used to produce a DIG (digoxigenin)-labeled RNA anti-sense probe (Roche DIG RNA Labelling Kit (SP6/T7)) ...
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bioRxiv - Immunology 2021Quote: ... concentrations measured in 100 ul of basolateral medium incubated with the reaction mixture of a cytotoxicity detection kit (Sigma-Aldrich, Roche, Saint Louis, MO, USA) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... A LYS2 probe was amplified and labeled with Digoxigenin (DIG)-dUTP using the primers 5’-TGAAGCCTTCCCAGAGAGAA and 5’-GCCAAGGAAAAATGTCTACCA (Roche PCR DIG Probe Synthesis Kit). The DIG probe was hybridized to the membrane (at 44°C ...
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bioRxiv - Microbiology 2020Quote: ... The three PCR reactions per sample were combined and purified using the High Pure PCR Product Purification Kit of ROCHE™ (Roche Diagnostics GmbH, Mannheim, Germany). Sequencing was performed using the MiSeq™ Illumina® (2 x 300 bp ...
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bioRxiv - Immunology 2021Quote: ... The tissues were processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159).
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bioRxiv - Immunology 2022Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760– 159). All tissue slides were evaluated by a study-blinded board-certified veterinary pathologist.
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bioRxiv - Immunology 2023Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760–159). Detection of SARS-CoV-2 viral RNA was performed using the RNAscope 2.5 VS assay (Advanced Cell Diagnostic Inc. ...
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bioRxiv - Microbiology 2023Quote: ... The tissues were then processed for immunohistochemistry using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat. 760-159).
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bioRxiv - Plant Biology 2023Quote: First-strand cDNA was synthesized by reverse transcribing 1 μg of total RNA in a final reaction volume of 20 μl using MMLV reverse transcriptase (Roche High Fidelity cDNA synthesis kit) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Quantitative RT-PCR was performed using the Qiagen kit QuantiFast® SYBR® Green RTPCR in a LigthCycler® 480 instrument (Roche Molecular Systems, Inc.) with mouse gene-specific oligonucleotides (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... spectrophotometer and 100 ng to 300 ng of total RNA was retrotranscribed to cDNA using the First Strand cDNA synthesis kit with random hexamers (Roche, catalog 04 897 030 001), or the High-Capacity cDNA Reverse Transcription Kit with RNAse inhibitor from Applied Biosystems (catalog ...
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bioRxiv - Cell Biology 2021Quote: ... staining was performed by autostaining on a Discovery Ultra Ventana using the Discovery OmniMap anti-Rb HRP and Discovery ChromoMap DAB kits (Roche, Cat # 760-4311 and 760-159).
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bioRxiv - Microbiology 2023Quote: ... no-chloroplast amplifying primers 335F (CADACTCCTACGGGAGGC) and 769R (ATCCTGTTTGMTMCCCVCRC) (Dorn-In et al. 2015) were used together with KAPA2G Robust PCR Kits (Kapa Biosystems, Cat. No. KK5005, Wilmington, USA). In protocol 2 (P2) ...
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bioRxiv - Plant Biology 2023Quote: ... and 1.5 ug RNA was used for library preparation at the High-throughput Sequencing Facility at UNC Chapel Hill using the Stranded mRNA-Seq kit (Kapa Biosystems. The NovaSeq 6000 sequencer (Illumina) generated 50bp paired-end reads with a read depth of 23-35 million reads per biological replicate ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The primers were appended to the DNA molecules by a 9-cycle PCR with the KAPA HiFi PCR Kit (Kapa Biosystems Pty, Cape Town, South Africa) with cycling parameters of 72°C for 3 min for gap-filling and 98°C for 30 s for denaturation ...