Labshake search
Citations for Roche :
5101 - 5150 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA from twenty-four females from each of the four groups was extracted using Roche High Pure™ PCR Template Preparation Kits (Roche Molecular Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Bulk RNA-seq libraries were prepared from 500 ng of total RNA for each sample using KAPA mRNA HyperPrep Kit for Illumina (Roche, #KR1352) following manufacturer’s instruction ...
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bioRxiv - Genetics 2022Quote: ... a single ChIP and single input library was prepared from each of three plants using a KAPA HyperPrep kit (Roche #KK8502), amplified with 5 cycles of PCR ...
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bioRxiv - Genomics 2022Quote: ... the fragments were treated with end-repair, A-tailing, and ligation of Illumina-compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit ...
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bioRxiv - Immunology 2022Quote: ... Library quantification and quality checks were done using KAPA Library Quantification Kit for Illumina (#KK4824; Kapa Biosystems, Cape Town, South Africa), High Sensitivity D1000 DNA Kit on BioAnalyzer (#5067-4626/#5190-6502 ...
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bioRxiv - Microbiology 2022Quote: ... The Kapa HyperPrep kit was used to prepare libraries from 50 μL of each sheared cDNA sample following modifications of the Kapa HyperPrep kit (version 8.20) and SeqCap EZ HyperCap Workflow (version 2.3) user guides (Roche Sequencing Solutions Inc.). Adapter ligation was performed for 1 hour at 20°C using the Kapa Unique-Dual Indexed Adapters diluted to 1.5 μM concentration (Roche Sequencing Solutions Inc.) ...
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bioRxiv - Microbiology 2022Quote: ... Following DNA extraction Illumina shotgun sequence libraries were prepared using the Kapa HyperPrep kit according to manufacturer specifications (Roche; Basen, Switzerland). Libraries were sequenced on an Illumina NovaSeq S4 flow cell (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... genomic DNAs were fragmented using a Covaris S220 ultrasonicator and libraries were generated using KAPA LTP Library Preparation Kit (Roche, KK8230). To verify that the insertion was present in the genome at the correct location in both transfected lines ...
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bioRxiv - Microbiology 2022Quote: ... hybridization and signal detection were carried out according to the instructions of the manufacturer (DIG RNA labelling kit and detection chemicals; Roche Diagnostics).
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bioRxiv - Microbiology 2022Quote: ... automated extraction of total nucleic acids was performed on the MagNA Pure 96 system with the DNA and Viral NA Small Volume 2.0 kit (Roche, Meylan, France). HBV-DNA was quantified in IU/mL (WHO Standard ...
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bioRxiv - Plant Biology 2022Quote: ... The libraries were constructed at the Genomics Core Facility at West Virginia University using the KAPA mRNA Stranded Kit (KAPA Biosystems), and then 150-bp paired-end sequenced at the BioHPC Lab at Cornell University using an Illumina NextSeq 500 platform ...
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bioRxiv - Systems Biology 2022Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the KAPA mRNA HyperPrep Kit (KAPA Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina) (Kapa Biosystems), using 10 cycles of PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting was conducted to confirm the correct deletion using the digoxigenin (DIG) high prime DNA labeling and detection starter Kit I (11745832910 Roche Germany). The Myb gene complementation vectors were constructed by cloning the entire length of the target gene with the native promoter region (about 1.5-kb ...
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bioRxiv - Cell Biology 2023Quote: Library preparation was performed with 500 ng of total RNA where the ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems), fragmented in reverse transcriptase buffer and heated to 94°C for 8 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR (hereafter called qPCR) was performed by using the KAPA SYBR FAST qPCR Kit Master Mix (2×) Universal (KAPA Biosystems) with StepOne plus (Applied Bio systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... The pooled samples with different indices for multiplex sequencing were measured using the library quantification kit (Kapa Biosystems, Wilmington, MA, USA) to obtain molar concentrations ...
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bioRxiv - Systems Biology 2022Quote: ... CRISPR gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). In the first PCR step ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The libraries were diluted 1:10 000 and 1:20 000 and quantified in triplicate using the KAPA Library Quantification Kit (Kapa Biosystems). Equimolar samples were pooled ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of RNA was reverse-transcribed using the Transcriptor First Strand cDNA Synthesis Kit with random hexamer primers (Roche, 04379012001), and a 370-bp fragment (nucleotides 1304- 1673 of NM_010610.3 ...
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bioRxiv - Genomics 2023Quote: ... and Illumina PCR-free libraries were prepared from 700 ng DNA using the KAPA Hyper prep kit and unique dual-indexed adapters (5 µL of a 15 µM stock) according to the supplier’s protocol (Roche, Basel, Switzerland). The library concentration and size distribution were assessed on a Bioanalyzer (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: Plasmids were obtained from Transomic Technologies and the antisense mRNAs were synthesized using the Digoxigenin RNA Labeling Kit (SP6/T7; Roche, 11175025910). DIG RNA in situ hybridization (ISH ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were then quantified at the Genome Quebec Innovation Centre (Montreal, Quebec) using a KAPA Library Quantification kit (Kapa Biosystems, USA), and average fragment size was determined using a LabChip GX (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with a KAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In house adaptors were ligated to 100-300 bp fragments of dsDNA ...
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bioRxiv - Immunology 2023Quote: ... DNA was incubated for 10 minutes at 65°C before performing RT qPCR using the KAPA SYBR FAST qPCR Kit from KAPA Biosystems. Primers used for real-time qPCR can be found in Supplementary Table 1.
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bioRxiv - Evolutionary Biology 2024Quote: ... and amplified 6 times (Sets 1 and 2) or 8 times (Set 3) with a KAPA Library Amplification Kit (KAPA Biosystems). The amplification products were cleaned using Agencourt Ampure XP reagent or KAPA Pure Beads ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were pooled in equimolar amounts and final library quantification performed using the KAPA Library Quantification Kit for Illumina (Roche; KK4824).
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bioRxiv - Genetics 2024Quote: ... along with a single -RT sample for each corresponding biological replicate using 2 μl cDNA and a no template control (NTC) with the LightCycler® 480 SYBR Green I Master kit (Roche). Ct values were measured with the Bio-Rad C1000 CFX96 Real-Time System and Bio-Rad CFX Software (qPCR primers ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Genomics 2024Quote: ... and then by real-time PCR against a standard curve using the KAPA Library Quantification Kit (Kapa Biosystems/Roche, Basel, Switzerland). Libraries were sequenced on an Illumina NovaSeq 6000 platform with S1 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was quantified and poly(A) selected RNA-seq libraries were generated using a KAPA Stranded mRNA-seq kit (KAPA Biosystems) with 500 ng RNA per sample as starting material ...
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bioRxiv - Cancer Biology 2024Quote: ... Relative gene expression was determined using the Quanta SYBR Green Master Mix Kit (Quanta Bio) on a LightCycler 96 (Roche Diagnostics), using GAPDH as the reference gene.
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bioRxiv - Neuroscience 2024Quote: ... and fluorescein (FITC)-labeled mRNA against Dbh using reverse-transcribed mouse cDNA as a template and an RNA DIG or FITC-labeling kits from Roche (Sigma). Primer and probe sequences for the Slc17a7 (Vglut1) ...
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bioRxiv - Cell Biology 2024Quote: ... samples were covered with the staining mixture at 37°C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Genetics 2023Quote: ... qPCR was performed in a final volume of 20 μl containing 20 ng of total DNA using SYBR Fast Universal qPCR Kit (Kapa Biosystems) and analysed using the Quant Studio 6 Flex system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... Digoxigenin (DIG)-labeled anti-sense mak RNA probe was generated by in vitro transcription from this T7 promoter: mak cDNA fragment as the template using a DIG RNA labeling kit (Roche, 11175025910). Zebrafish embryos from the 1-cell stage to 72 hpf were fixed with 4% PFA at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... One μg of total RNA was used for polyA-RNA isolation and library preparation using the KAPA mRNA Hyperprep kit (Roche, KK8580) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Library preparation of isolated RNA from Flag-PCBP1 immunoprecipitations or from whole cell lysates (inputs) was performed with the KAPA RNA Hyperprep Kit with RiboErase (Roche, #KK8560) using NEBNext Multiplex Oligos (adaptors and single index primers ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of specific gene transcripts was achieved through droplet digital PCR (ddPCR) using the Digital LightCycler 5× RNA Master Kit (Roche, swiss) with specific primers and probes ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were produced by conducting seven PCR cycles using a KAPA LTP library preparation kit (Roche Sequencing and Life Science) with NEBNext multiplex oligos for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... DNA_P4 was MagNA Pure 96 DNA and Viral NA Small Volume Kit on the MagNA Pure 96 Instrument (Roche, Basel, Switzerland) with proteinase K pre-treatment step and standard buffers using the DNA Blood ds SV Protocol optimized for double stranded DNA and Next Generation Sequencing (Table 1) ...
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bioRxiv - Cell Biology 2024Quote: ... Apoptotic cells (including fragmented DNA) were detected using the In Situ Cell Death Detection Kit POD (11684817910, Roche Ltd., Basel, Switzerland). To avoid false-positive or false-negative results ...
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bioRxiv - Genomics 2024Quote: ... The duplicate reactions were pooled and the libraires were enriched for viewpoints of interest in a double capture procedure using the KAPA Hyper Capture Reagent Kit (Roche, 9075828001). A total of 8 µg of indexed sample per biological replicate was used as input for the first capture ...
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bioRxiv - Immunology 2024Quote: ... Poly(A)-enriched cDNA libraries were generated using the Illumina TruSeq sample preparation kits and quality and quantity verified using the bioanalyzer and qPCR (KAPA Biosystems). Using dual-index barcoding methods to reduce index hopping ...
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bioRxiv - Immunology 2024Quote: ... Indexed libraries that meet appropriate cut-offs for both are quantified by qRT–PCR using a commercially available kit (KAPA Biosystems). Insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... PN-1000250).The concentration of each library was accurately determined through qPCR using the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to achieve the desired cluster counts for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Physiology 2024Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... The final libraries were analyzed using the Agilent bioanalyzer and the libraries were quantified using the Kapa SYBR FAST Universal qPCR kit for Illumina sequencing (Kapa Biosystems). The individual libraries were diluted to 6 nM and 2 uL of each added to a library pool ...
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bioRxiv - Immunology 2024Quote: ... cDNA was reverse transcribed using a HiScript III 1st Strand cDNA Synthesis Kit (Vazyme) and qPCR was performed using ChamQ SYBR® qPCR Master Mix (Vazyme) with a LightCycler 96 instrument (Roche). The relative mRNA expression was calculated by the value of 2-ΔCt normalized to 18S ...
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bioRxiv - Molecular Biology 2024Quote: ... Real-time PCR was performed to assess the concentration of target rRNA using SYBR Green Master Mix kit (Roche, Basel, Switzerland) in accordance with the manufacturer’s recommendations on LightCycler® 96 Real-Time PCR System (Roche ...