Labshake search
Citations for Roche :
5451 - 5500 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche cat# KK4824) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Microbiology 2024Quote: ... a relative qPCR assay using anammox-specific 16S rRNA and general 16S rRNA genes was performed with KAPA SYBR® FAST qPCR Master Mix (2X) Kit (Kapa Biosystems, Inc.). The primers used for the amplification of anammox-specific 16S rRNA genes (Anammox 16S ...
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bioRxiv - Genomics 2024Quote: ... the cell pellet was used to extract genomic DNA (gDNA) using the High Pure PCR Template Preparation Kit (Roche Molecular Systems, Inc., Switzerland). Afterwards ...
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bioRxiv - Immunology 2024Quote: Five hundred nanogram of RNA from CD4+T cells underwent bulk RNA-seq using KAPA stranded mRNA-Seq kit (KAPA Biosystems, Wilmington, MA). Transcripts were aligned to hg38 assembly using STAR aligner (v2.4.2a)63 ...
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bioRxiv - Molecular Biology 2024Quote: Bacterial DNA was extracted from human fecal samples using phenol-chloroform extraction method with glass beads and purified using a High Pure PCR Template Preparation Kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted from primary intestinal epithelial cells and organoid cultures using the High Pure RNA Isolation Kit (Roche Life Science, Indianapolis, IN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by RT-qPCR using a commercially available kit (KAPA Biosystems; Roche Basel, Switzerland) and insert size distribution determined with the LabChip GX or Agilent Bioanalyzer.
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bioRxiv - Cancer Biology 2024Quote: ... Strand-specific RNA libraries were prepared for sequencing (3 - 4 biological replicates/treatment) using a KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, MA, USA). Poly-A mRNAs were purified from 100 ng of total RNA using poly-T-oligo-magnetic beads (Kapa Biosystems ...
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bioRxiv - Evolutionary Biology 2024Quote: ... A small subset of samples was sent to an external collaborator by the Molecular Evolution Core where library preparation was performed using the KAPA HyperPrep Kit (Roche, Material Number: 07962363001). Samples across all runs were sequenced on a NovaSeq 6000 instrument using v1.5 chemistry.
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bioRxiv - Evolutionary Biology 2024Quote: ... We prepared dual-indexed libraries with custom indexes (Glenn et al. 2019) and the Kapa Biosystems Hyper Prep Plus library preparation kit (F. Hoffmann-La Roche AG, Basel, Switzerland) following the manufacturer’s protocol at one-half volume.
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was purified from homogenized tissues using a combination of Trizol (Tri Reagent; Molecular Research Centre, Burlington, ON, Canada) and a High Pure RNA isolation kit (Roche Scientific, Laval, QC, Canada) as described previously (30) ...
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bioRxiv - Developmental Biology 2020Quote: Digoxigenin (DIG)-labeled and Fluorescein (Flu)-labeled in-situ probes were synthesized using in vitro transcription with Flu/DIG RNA Labeling kits respectively (Roche,Cat#11175025910, Cat#11685619910). RNA in-situ hybridization was conducted according to the published protocol 85 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5% CO2 by quantification of LDH released into cell supernatants by apoptotic/necrotic cells (LDH detection kit, Roche Applied Science, #11 644 793 001). Maximal lysis of the target cells (= 100% ...
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bioRxiv - Cancer Biology 2020Quote: ... The captured libraries were quantified using KAPA Library Quantification Kits for Illumina Sequencing platforms according to the qPCR Quantification Protocol Guide (Kapa Biosystems, catalog number KK4854) and qualified using the TapeStation D1000 ScreenTape assay (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... total nucleic acid was extracted from 400 µl of cerebrospinal fluid using the MagNA Pure Compact Nucleic Acid Isolation Kit I (Roche Diagnostics, Indianapolis, IN, USA) on the MagNA Pure compact automated extractor ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were pooled in equimolar amounts after library quantification using both quantitative PCR with the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA, USA) and the fluorometric Qubit dsDNA high sensitivity assay kit (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: DNA protein interaction was studied with purified H-NS and DIG labeled PCR amplified PctxAB fragments by using the DIG gel shift kit (2nd Gen, Roche Aplied Science, Mannheim, Germany) as previously described (10) ...
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bioRxiv - Molecular Biology 2021Quote: ... Barcode-indexed sequencing libraries were generated from reverse-crosslinked ChIP-DNA samples using a Kapa Hyper DNA Library Preparation Kit (Kapa Biosystems-Roche, Basel, Switzerland) and NextFlex UDI adapters (PerkinElmer ...
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bioRxiv - Neuroscience 2022Quote: ... and indexed libraries were created for sequencing using Truseq adapters and quantified using the Kapa universal qPCR library quantification kit (Kapa Biosystems Inc., MA, USA). Samples were sequenced on an Illumina Novaseq using a 2×100 paired-end chemistry kit (Illumina Inc. ...
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bioRxiv - Biophysics 2022Quote: ... The PVDF membranes were developed using an enhanced chemiluminescence western blot detection kit (Pierce SuperSignal® West Dura, Rockford, IL) and exposed to Lumi-Film chemiluminescent detection films (Roche Diagnostics, Mannheim, Germany). The experiment was replicated five times ...
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bioRxiv - Cancer Biology 2022Quote: A total amount of 1 μg total RNA per sample was used for sequencing libraries generation by using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was conducted using an in-situ cell death detection kit (Roche, Indianapolis, Indiana, United States) as per the manufacturer’s instructions [31,34] ...
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bioRxiv - Neuroscience 2021Quote: ... genomic DNA was isolated from the biosamples using magnetic beads with the MagNA PureLC DNA Isolation Kit—Large Volume (Roche Diagnostics GmbH., Mannheim, Germany). Genotypes for CYP2C19 ...
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bioRxiv - Plant Biology 2022Quote: ... of total and polysomal transcripts was conducted as described in [96] with the difference of using KAPA SYBR® FAST qPCR Kit (Kapa Biosystems, South Africa). ACTIN2 (AT3G18780 ...
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bioRxiv - Microbiology 2020Quote: ... RNA was reverse transcribed using Transcriptor High Fidelity cDNA Synthesis Kit and a specific 3’-UTR DENV-1 primer (Roche Applied Science, Mannheim, Germany), d1a5B 5’-AGAACCTGTTGATTCAACRGC-3’ (62) ...
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bioRxiv - Genetics 2020Quote: ... The final linked-read library (8 cycles of PCR amplification) was quantified using the Kapa Library Quantification Kit (Cat. No. KK4824) from Kapa Biosystems (Woburn, MA, USA) and diluted to 4 nM ...
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bioRxiv - Plant Biology 2021Quote: ... was isolated from the cDNA of juvenile cluster root tissues from P-deficient plants using the 5’/3’ RACE Kit (2nd generation, Roche Diagnostics S.p.a., Monza, Italy) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The RNA-Seq Library was prepared according to KAPA Stranded mRNA-Seq poly(A) selected kits with 201-300 bp insert sizes (KAPA Biosystems, Wilmington, MA, USA) using 250 ng total RNA as an input ...
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bioRxiv - Genomics 2020Quote: ... nucleic acids were extracted directly from 200 μl CSF sample using the MagNApure 96 DNA and Viral NA Small volume extraction kit on the MagNAPure 96 system (Roche Diagnostics, Almere, The Netherlands) with 100 μL output eluate ...
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bioRxiv - Microbiology 2020Quote: ... the genomic DNA of each isolate was extracted using a MagNA Pure LC DNA isolation kit on a MagNA pure LC instrument (Roche Diagnostics GmbH, Mannheim, Germany), which was used for Nextera XT library construction and genome sequencing (300 bp paired-end ...
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bioRxiv - Developmental Biology 2020Quote: ... Riboprobes were synthesized from the plasmids by in vitro transcription using either T7 or Sp6 polymerase and a digoxigenin (DIG) labeling kit (Roche Applied Science, Indianapolis, IN). The primer sequences used for riboprobe preparation are given in Supplemental Table 1.
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bioRxiv - Molecular Biology 2020Quote: Equal amount by mole of non-targeted and targeted RNA-Seq library were mixed based on qPCR-based quantification with Kapa Library Quantification Kit (KAPA BIOSYSTEMS, Wilmington, MA, USA). Sequencing of 150-bp paired-ends using HiSeq was carried out.
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bioRxiv - Genomics 2021Quote: ... Quantification of final library before loading on MiSeq sequencer was performed using the Kapa quantification kit (RT-PCR) for next generation sequencing with the Illumina platform (Kapa Biosystems, Wilmington, MA USA). Sequence reads were quality filtered and assembled using CLC Genomics Workbench 8.0.1 (CLCbio ...
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bioRxiv - Genomics 2021Quote: ... Sense and antisense digoxigenin-labelled RNA probes were generated by in vitro transcription using the DIG-RNA Labeling Kit (SP6/T7; Roche Applied Sciences, QC, Canada). Labeled RNA probes were quantified by dot blot analysis.
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bioRxiv - Genetics 2020Quote: ... Tag sequences for identifying individual trees were attached to the DNA fragments using the Access Array Barcode Library for Illumina Sequencers-384, Single Direction (Fluidigm Corporation, South San Francisco, CA, USA) and KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, Wilmington, MA, USA). PCR was performed in a 20 μL volume containing 2 μL of 20-times diluted PCR products ...
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bioRxiv - Microbiology 2022Quote: ... IHC tissues were processed using the Discovery Ultra automated stainer (Ventana Medical Systems) with a ChromoMap DAB kit (Roche Tissue Diagnostics cat#760-159) for immunohistochemistry (IHC ...
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bioRxiv - Neuroscience 2022Quote: ... tissue samples were taken by collecting ∼2 mm of the end of the tail and extracting DNA from the tissue sample using the Kapa Express Extract Kit (Kapa Biosystems, Wilmington, MA, USA). The extracted DNA was then subjected to PCR using in-house designed primers to identify WT and KO alleles ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... n=40) and brain (n=40) for each individual using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adapters (KAPA Biosystems; Wilmington, MA, USA), with 100 ng of large RNA as input ...
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bioRxiv - Genetics 2022Quote: ... and amplifying the exon regions of targeted genes by using the primers with SP6 sequence (ATTTAGGTGACACTATAGAAGNG) according to the product description of KAPA HiFi PCR Kit (Roche Diagnostics, cat. no. 07958927001). Sense and antisense digoxigenin (DIG)-labeled probes were synthesized from the purified PCR product using DIG RNA Labeling Kit (Roche ...
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bioRxiv - Genetics 2022Quote: ... The hygromycin cassette including the B-tubulin promoter and terminator was amplified from pPHT1 plasmid with the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, MA, USA). The PCR thermocycling profile used was as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: ... first purified with two rounds of polyA mRNA selection with oligo-dT magnetic beads and then converted to sequencing-compatible cDNA using the KAPA mRNA HyperPrep kit (KAPA biosystems, Cat. No. KK8580), using the KAPA single-indexed adapter kit for multiplexing (KAPA biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting cDNA was used for qRT□PCR analyses using the TransStart® Green qPCR SuperMix kit (AQ101-01, TransGen Biotech, Beijing, China) on a LightCycler® 480 System (Roche, Basel, Switzerland). Ubiquitin 5 was selected as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... Final libraries were analyzed using Agilent DNA 1000 chip to estimate the quantity and check size distribution and were then quantified by qPCR using the KAPA Library Quantification Kit (ref. KK4835; Kapa Biosystems, Wilmington, MA, USA) prior to amplification with Illumina’s cBot ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted DNA samples were sent to Admera Health (South Plainfield, NJ) and were prepared using the KAPA HyperPrep kit (Kapa Biosystems, Inc., Wilmington, MD) according to the provided instructions ...
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bioRxiv - Genetics 2024Quote: ... Strand-specific and dual-barcode indexed RNA-seq libraries were generated from 450 ng total RNA each using the Kapa RNA-seq Hyper kit (Kapa Biosystems-Roche, Basel, Switzerland) and both the QIAseq FastSelect–5S/16S/23S ribodepletion and FastSelect rRNA Plant reagents (Qiagen ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (5∼8 μg) for Nanopore sequencing was end-repaired and ligated via a KAPA Hyper Prep Kit (Cat#KR0961, Kapa Biosystems, Wilmington, MA, USA), following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We verified enrichment success and measured size-adjusted DNA concentrations of each pool with qPCR using a SYBR-FASTqPCR kit (Kapa Biosystems, Wilmington, MA, U.S.A.) and a Bio-Rad CFX96 RT-PCR thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were processed for DNA strand breaks using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) from Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... 1 μg of RNA from each sample was processed using the KAPA Stranded mRNA-Seq Kit with mRNA capture beads (Kapa Biosystems, Bedford row, London). Each sample was then eluted in 20 μL of elution buffer adjusted to 10 mM ...
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bioRxiv - Neuroscience 2023Quote: Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA). Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit (Qiagen ...