Labshake search
Citations for Roche :
5251 - 5300 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and prepared for shotgun metagenomic sequencing using the Kapa Hyperprep Plus kit with 3 rounds of PCR amplification (Kapa Biosystems, Wilmington, MA), and sequenced to an average depth of 7 gbp per fraction on the Illumina NovaSeq (Illumina ...
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bioRxiv - Genomics 2021Quote: ... and then either the remaining components of the PowerSoil Pro kit or DNA purification beads from Kapa Biosciences (Roche, Burgess Hill, UK) and either the MPBio SuperFastPrep-2 (MPbio ...
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bioRxiv - Cancer Biology 2021Quote: ... Femurs were then decalcified and paraffin sections (5-μm thickness) were stained with hematoxylin and eosin or reticulin (Reticulum II Staining Kit, Roche, Tucson, AZ) to assess fibrosis ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Microbiology 2020Quote: Each 300 μL of GEA supernatant sample was extracted using the High Pure PCR Template Preparation Kit (Roche Life Science, Mannheim, Germany), a kit based on silica-membrane spin columns technology ...
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bioRxiv - Molecular Biology 2021Quote: ... the probe for RNA FISH was prepared with 2 µg of BAC RP183A17 using Nick translation kit (10976776001) purchased from Roche (Basel, Switzerland). Salmon sperm DNA and cot1 DNA were added to the probe to mask the repetitive sequence ...
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bioRxiv - Immunology 2020Quote: ... CD206 and CD64 was performed by Fast Start DNA Masterplus SYBR Green I kit on the Light Cycler 480 (Roche, Applied Science). Relative RNA expression levels were normalized to the geometric mean of two reference genes RPL37A and ACTB ...
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bioRxiv - Immunology 2021Quote: ... it was added 50 µL of fresh TUNEL reaction mixture composed of 5 µL of enzyme solution mixed with 45 µL of labeling solution (In Situ Cell Death Detection kit, POD, ROCHE, version 15.0) for 1 hour at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... cultured in vitro at 30 °C and at 37 °C in EMJH medium was also extracted using a High Pure RNA Isolation kit (Roche Applied Science) following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... The membrane was hybridized using digoxigenin-labeled DNA probes (S4 Table) for mPSP based on the DIG-High Prime DNA Labeling and Detection Starter Kit II protocol (Roche, Mannhein, Germany). For western blotting ...
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bioRxiv - Immunology 2023Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche, cat#07960140001). Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis and sequenced on a NovaSeq 6000 with a paired-end 150 cycle sequencing run.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated at 37 °C in the dark for 1 h with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) solution from the In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). The embryos were washed three times with PBS-PVP ...
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bioRxiv - Microbiology 2023Quote: ... total RNA was isolated by RNA Premium Kit (NIPPON Genetics, Cat# FG-81250) and cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) with random hexamer ...
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bioRxiv - Cancer Biology 2023Quote: ... Strand-specific and barcode-indexed RNA-Seq libraries were generated from 300 ng total RNA each after poly-A enrichment using the mRNA-Seq Hyper Kit (Kapa Biosystems, KK8581) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and 10 ng of purified DNA for each IP and input sample were used for library preparation with the KAPA Hyper Prep Kit (Kapa Biosystems KR0961). TAZ and Combo treatments required two IPs per biological replicate to attain enough material for library preparation ...
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bioRxiv - Microbiology 2023Quote: For NGS library preparation we added adapters and unique dual indices in a two-step amplification process using the KAPA HiFi HotStart Library Amplification Kit (KAPA Biosystems, KK2611) and purified samples with Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... The libraries were quantified by fluorometry on a Qubit instrument (LifeTechnologies) and by qPCR with a Kapa Library Quant kit (Kapa Biosystems-Roche) prior to sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA inserts of the plasmids were isolated and amplified by low-cycle (12x) PCR using a Kapa2G Robust Hotstart PCR kit (Roche, Ref #07961073001) according to specifications ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from the cells was collected and used to quantify LDH release using the LDH cytotoxicity detection kit (Roche, Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... or T3 polymerase (pSC-best4, pSC-otop2) and fluorescein or digoxygenin RNA labeling kits (digoxygenin: Roche, Cat# 11277073910; fluorescein: Roche, Cat# 11685619910) These reactions were cleaned using the NEB Monarch RNA Cleanup protocol (NEB ...
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bioRxiv - Pathology 2023Quote: Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine nick-end labeling (TUNEL) staining was performed by using the In Situ Cell Death Detection Kit from Roche (Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The libraries obtained from the CBE approach were quantified using the qPCR KAPA library quantification kit (Roche Diagnostics, Cape Town, South Africa). Sample size of all libraries was checked using an Agilent 2100 Bioanalyzer (Agilent technologies ...
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bioRxiv - Genomics 2023Quote: ... Then DNA fragments underwent a NGS library preparation procedure consisting in end repair and Illumina adaptor ligation using the KAPA HTP kit (Roche, Basel, Switzerland). Individual index sequences were added to each library for identifying reads and sorting them according to their initial origins ...
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bioRxiv - Microbiology 2024Quote: ... Total nucleic acids were extracted from 200 µl of each sample using the DNA/Viral NA Small Volume v2.0 extraction kit (Roche Diagnostics, Mannheim, Germany) and an automated extractor MagNA Pure 96 ...
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bioRxiv - Physiology 2022Quote: ... The PCR products of target genes were purified and used as templates for probe preparation using DIG or fluorescein RNA labeling kits (Roche, Basel, Switzerland). The pituitary sections were hybridized with the antisense RNA probes for fshb and lhb as we previously reported [52] ...
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bioRxiv - Cell Biology 2022Quote: cDNA was synthesized from 100–500 ng of total RNA in 10 µl reactions with a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol using random hexamer primers and an MJ Mini Personal thermal cycler (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System, Laval, QC, Canada) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Exome libraries were prepared from 1 μg of genomic DNA from each analyzed section using the Nimblegen EZ Exome kit V3 (Roche, Nutley, NJ). Paired-end 100 bp sequencing was performed on a HiSeq2500 sequencer (Illumina Inc. ...
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bioRxiv - Physiology 2024Quote: ... 20 ng cDNA was used for real-time qPCR reactions with KAPA SYBR FAST qPCR Kit (Catalog no. KK4618, Roche, Basel, Switzerland) using a CFX Opus Real-Time PCR System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2024Quote: ... Gene-specific antisense or sense DIG-labeled cRNA probes were obtained by in vitro transcription with T7 polymerase using the Roche DIG RNA labeling kit (Roche Diagnost ics) following the instructions of the supplier ...
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bioRxiv - Cancer Biology 2024Quote: ... size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480). Libraries were sequenced using an Illumina Novaseq 6000 sequencer as single-end 100 base reads ...
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bioRxiv - Neuroscience 2024Quote: ... cDNAs and final libraires were run on an Agilent 4200 TapeStation to check for quality and were quantified using KAPA Library Quantification Kit (Roche, Basel, Switzerland).
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bioRxiv - Genomics 2024Quote: ... Hybridization was carried out with SeqCap RNA Choice Probes using the KAPA HyperCapture Reagent and Bead kit (v2, Roche Sequencing Solutions, Inc.) according to SeqCap RNA Enrichment System User’s Guide (v.1.0 ...
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bioRxiv - Cancer Biology 2024Quote: Terminal dUTP Nick End Labeling (TUNEL) staining was performed with an In Situ Cell Death Detection Kit (TMR Red #12156792910; Roche Applied Science) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Illumina-compatible sequencing libraries were prepared from 20 ng of purified DNA in 50 μL of TE buffer using the KAPA Hyperprep Kit with Library Amplification Primer Mix (Roche, CAT #07962363001) and 6 cycles of PCR amplification ...
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bioRxiv - Cancer Biology 2024Quote: ... we treated sensitive and resistant cells with increasing concentrations of SP600125 for 24 hours and evaluated the cell viability using the Cell Proliferation Kit I (MTT) (Roche, Cat. 11465007001) and measuring the absorbance value at λ=590nm ...
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bioRxiv - Physiology 2023Quote: ... 500ng of total purified RNA was used for library preparation with the KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina Platforms (KAPA Biosystems KK8561) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared by the Van Andel Institute Genomics Core from 500 ng of total RNA using the KAPA stranded mRNA kit (v4.16) (Kapa Biosystems, Wilmington, MA). RNA was sheared to 300-400 bp ...
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bioRxiv - Physiology 2024Quote: ... RNA probes were synthesised and labelled using the T7-ScribeTM Standard RNA IVT Kit (Madison, Wisconsin, United States) and Digoxigenin-11-UTP (Roche, Basel, Switzerland). Embryos at various stages were fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... For this 2 µl of RNA sample were mixed with the PCR ingredients of the one-step LightCycler 480 RNA Master Hydrolysis Probes kit (Roche Holding AG), the JFH1-specific probe (5’-6FAM-AAA GGA CCC AGT CTT CCC GGC AA-TMR-3’ ...
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bioRxiv - Microbiology 2023Quote: One microgram of RNA of each isolate was converted into cDNA using the Transcriptor high-fidelity cDNA synthesis kit (Roche, Basel, Switzerland). RT-PCR was performed in 96-well plates using the PowerUp SYBR green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of ligated fragments in each library was quantified with the KAPA Library Quantification Kits for Illumina platforms (Roche/KAPA Biosystems, KK4824) on a Roche LightCycler 480 Instrument (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... The bulk transcriptome of these cases was obtained after macrodissection of the whole tumor area on the same block as the one used for the IHC and obtained from FFPE sections using a high-purity FFPE RNA isolation kit (Roche, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Cytotoxicity was assessed by measuring the release of lactate dehydrogenase (LDH) into the culture supernatant using the cytotoxicity detection kit PLUS (Roche, Basel, Switzerland). 100 μl cell-free supernatant ...
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bioRxiv - Microbiology 2023Quote: Transcriptional start sites (TSS) of rokL6 and sco1448 were determined by 5’ RACE using a 5’/3’ RACE Kit 2nd generation (Roche, California, US). Total RNA (2 μg ...
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bioRxiv - Biochemistry 2023Quote: ... Final quantification of the RNA-seq library was done with the Library Quantification Kit for Illumina® platforms (KAPA Biosystems, M.A, U.S.A.). The C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The primary antibody was either visualized with the multimer-technology based UltraView Universal DAB Detection kit (Ventana® BenchMark® XT; Roche) or a fluorochrome-labeled secondary antibody (Alexaconjugated ...
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bioRxiv - Pathology 2023Quote: Cell death was assessed in tissue sections using terminal deoxynucleotidyl transferase-mediated dUTP nick- end labeling (TUNEL, Roche TUNEL kit (12156792910; Roche, Basel, Switzerland), as previously described (Cade et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Deletion of FGSG_09786 was further verified by Southern blot with the Digoxigenin High Prime DNA Labeling and Detection Starter Kit I (Roche, Mannheim, Germany). Using a similar strategy ...