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Citations for Merck :
1551 - 1600 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: The cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Biophysics 2021Quote: ... cells were washed 2x 4 min in PBS and subsequently overlaid with 0.1 mg/ml collagen IV (C7521, Merck) and placed at 4°C overnight (ON) ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... One milliliter of the virus supernatants was directly overlaid on the cells in the presence of polybrene (Merck, Germany) at a final concentration of 4 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Then cells were washed 3 times with deionized water (Milli-Q® IQ 7003 Ultrapure Lab Water System, Merck), and dehydrated by washing them in a graded series of ethanol ...
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bioRxiv - Systems Biology 2020Quote: ... the cells were washed three times with PBS and saved in PBS supplemented with 0.02% sodium azide (Merck, S2002) at 4°C until image acquisition.
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bioRxiv - Microbiology 2021Quote: DF-1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... Apoptotic cells were identified by TUNEL assay on sections using an ApopTag in situ apoptosis detection kit (Merck-Millipore).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for SA-β-galactosidase activity overnight using a Cellular Senescence Assay kit (Merck Millipore, Massachusetts, USA) and imaged the next day in the motorised widefield microscope Cell Observer (Zeiss ...
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bioRxiv - Neuroscience 2022Quote: ... and plated in glass-coverslips (3000-4000 cells/well) pre-coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured on poly-L-ornithine and laminin-coated coverslips in 24-well plates at 150,000 cells per well in serum-free 2i Medium (Merck). After 24h ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed with 1x Laemmli (Bio- Rad Laboratories) with Benzonase nuclease and phosphatase inhibitors (Sigma-Aldrich p2850, Merck), and the lysates were migrated in 4–15 % gradient SDS-PAGE gels (Bio- Rad Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... when colony dispersion was observed and the cells were fixed and colored by Hemacolor® stain (Merck, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in 48-well plates (3000-4000 cells/well) previously coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Neuroscience 2020Quote: ... For electrophysiology experiments the excretory/secretory extracts were dialysed/buffer exchanged to PBS using an Amicon stirred cell (Merck) with a 3 kDa molecular weight cut-off membrane ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell extracts (500 μg per IP) were immunoprecipitated for 1h using a PHGDH-specific antibody (Merck Sigma, HPA021241) complexed with Dynabeads Protein A (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... we harvested the medium from the bottom compartment and counted the number of migrated cells using a Bürker-Türk counting chamber (Merck, BR719505). After cell counting ...
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bioRxiv - Microbiology 2022Quote: The growth of asexual blood stages was assessed by infecting NMRI mice at a parasitaemia of 0.01% and counting infected red blood cells in blood smears stained with Hemacolor (Merck). The percentage of total gametocytes was also determined by counting in blood smears ...
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bioRxiv - Immunology 2022Quote: ... Cells were then diluted to 200 μl with cold PBS and acquired using a GUAVA flow cytometer (Merck Millipore). Propidium Iodide (PI,Sigma ...
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bioRxiv - Cancer Biology 2022Quote: Lentiviral supernatants were generated into HEK293T cells by transient co-transfections of lentiviral CAS9-RFP Lenti Plasmid (Merck, USA) construct and appropriate amount of packaging vectors (Mission Lentiviral Packaging Mix ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Genetics 2020Quote: ... cells were filtered and resuspended from the filters (MF™ Membrane Filters 0.45 µm, Merck KGaA, 64293 Darmstadt, Germany) in the same volume of synthetic medium without serine ...
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bioRxiv - Biochemistry 2020Quote: Cells were harvested and lysed using RIPA lysis buffer (Beyotime, China) supplemented with 1% protease inhibitor cocktail (MERCK, Germany). After centrifugation at 14 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proportional volumes of bacterial and yeast cells were mixed and filtered through 0.45 µm mixed cellulose membrane (Merck, Millipore) before transferring onto IM agar supplemented with 200 µM acetosyringone ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was removed by centrifugation and the supernatant was filtered through a 0.22 µm syringe filter (Merck Millipore). Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: A 100μL bacterial suspension of 2.0 × 106 cells/mL was prepared and loaded on the four-chambered CellASIC ONIX B04A-03 microfluidic platform (Merck). Cells were trapped with the following pressure and flow time settings ...
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bioRxiv - Cancer Biology 2021Quote: ... cells and barcoded beads were respectively diluted at the concentration of 200 cell/μL in PBS with BSA 0.01% (Merck) and 120 bead/μL in lysis buffer ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... control cells were generated using the SHC202 -MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid (Merck). After infection ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Microbiology 2021Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... live cell fluorescence microscopy was performed as described before (85) with minor modification using 5 μg/ml Hoechst (Merck) to stain the nuclei ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... the obtained 2-cell stage embryos were transferred to a fresh drop of KSOM (Merck, cat. # MR-107-D). The IVF with b6d2F1 oocytes and b6casF1 has on average a 2-cell development (fertilization ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants were kept for viral titer determination and the cell pellets were harvested by Tri-reagent (Merck, Israel) for RNA purification.
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bioRxiv - Molecular Biology 2022Quote: Chicken embryonal fibroblast cells (DF1; a kind gift from Dr Dylan Sweetman) were expanded in standard Dulbecco’s Modified Eagle Medium (DMEM; Merck Life Science UK Limited ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells not set aside for surface antigen staining were incubated in PBS containing 0.1% Triton X-100 (X100, Merck) for 5 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were fixed on 4% PFA and senescence determination was made by measuring SA-β-gal activity (KAA02 Merck) and IF against the markers of senescence ...
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bioRxiv - Microbiology 2022Quote: ... and human embryonic kidney (HEK)293T cells (ATCC #CRL-3216) were maintained in Dulbecco’s Modified Eagle Medium with high glucose (Merck), supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured for at least 5 passages in DMEM containing either L- arginine and L-lysine (Merck) or L-arginine [13C6] and L-lysine [2H4] (Cambridge Isotope Laboratories).
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml, Merck, Cat.N. 3072) for the duration of the experiment ...
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation and pellets were suspended in PBST with 250 μg/mL lysozyme (Merck, Darmstadt, Germany) and lysed by three subsequent freeze-thaw-cycles ...