Labshake search
Citations for Merck :
1601 - 1650 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... HCT116 wild-type or p53 missense mutant cells were transfected with the plasmids using X-treme GENE HP (Merck) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... All cell lines were routinely tested for mycoplasma contamination using LookOut Mycoplasma qPCR Detection Kit (Merck 200-664-3). None of the cell lines were authenticated but low passage numbers were utilized.
-
bioRxiv - Microbiology 2023Quote: ... Parasitemia was determined daily following tail bleeds and assayed by automated fluorescent cell counting with a Muse cytometer (Merck-Millipore ...
-
bioRxiv - Genetics 2023Quote: ... The screens were performed without feeder cells in M15 medium supplemented with 1000 U/ml leukemia inhibitory factor (Merck). Cells were plated on flasks coated with 0.1% gelatin solution (Merck) ...
-
bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were resuspended in a modified sucrose electroporation buffer prepared by adding 272 mM sucrose (Sigma-Aldrich, Merck) to Opti-MEM™ Reduced Serum Medium (Life Technologies ...
-
bioRxiv - Genomics 2023Quote: ... The transduction was performed by adding the virus particle directly onto the cells supplemented with 6μg/mL polybrene (Merck). The cells were refreshed 24h after transduction and the antibiotics (puromycin/blasticidin ...
-
bioRxiv - Immunology 2023Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck) according to manufacturer’s specifications ...
-
bioRxiv - Cell Biology 2023Quote: 293T human embryo kidney (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (4.5 g/l glucose) (Merck, Dorset, UK) enriched with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation and pellets were suspended in PBST with 250 μg/mL lysozyme (Merck, Darmstadt, Germany) and lysed by three subsequent freeze-thaw-cycles ...
-
bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
-
bioRxiv - Systems Biology 2023Quote: ... For the multicellular spheroid generation 7.000 cells were seeded on ultra-low attachment 96-well plates (Corning CoStar, Merck). The spheroids were cultured for 72h at 37 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... LSEC were isolated by gradient density sedimentation of resting non-parenchymal cells on 25–50% Percoll (Merck Millipore, Germany) and purified by using CD146-based isolation on magnetic MicroBeads (MACS ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK-293T and HuTu-80 cells were transfected with the pSpCas9-GFP-LRP10gRNA plasmid using GeneJuice® reagent (Merck). After 48 h ...
-
bioRxiv - Neuroscience 2023Quote: ... MPAs or U373 cells were seeded in DMEM (catalogue # 11966) with 7.5mM glucose (catalogue # G7021, Merck Life Sciences (UK)) ...
-
bioRxiv - Immunology 2023Quote: Cells were infected separately with five different lentiviral transduction particles (at MOI = 5) containing five different shRNA species (Merck) specific for the mouse Flot2 gene (NM_008028 ...
-
bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
-
bioRxiv - Developmental Biology 2024Quote: mESCs were plated at a final density of 1 × 104 cells/cm2 on fibronectin-coated (MM Merck Millipore, FC010) µ-Slide 8 Well Glass Bottom Chamber Slides (Ibidi ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were washed once with PBS and then incubated with RPMI containing 1 µg / ml propidium iodide (PI) (Merck) and nigericin (Merck ...
-
bioRxiv - Genomics 2024Quote: ... Cells were then resuspended in 100µL RPMI + 10% FCS and stimulated for 4h with PMA (100ng/mL, Merck, P8139) and ionomycin (1µg/mL ...
-
bioRxiv - Immunology 2024Quote: ... For FACS sorted cells RNA was amplified cDNA synthesized using a SeqPlex RNA Amplification kit (Merck Life Science, UK) as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
-
bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... we re-analyzed microarray-based transcriptome datasets: i) HepG2 cells treated with vehicle (0.0125% DMSO) or 12 μg/ml silymarin (Merck) for 24 h (n = 3 biological replicates)98 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was then lysed in 5X volumes of Lysis Buffer (300μL) [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
-
bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with lentiviral particles in the presence of polybrene (8 g/mL, Cat# TR-1003-G, Merck); after 48 hours of transduction ...
-
bioRxiv - Cancer Biology 2024Quote: ... reflecting cell-number and -viability) was assessed using the MTT [Thiazolyl Blue Tetrazolium Bromide] assay (Sigma/Merck, M5655-500mg) according to manufacturer’s instructions and sigmoidal dose-response curves were generated to calculate the mean IC50 values of each drug that were used in the subsequent study ...
-
bioRxiv - Bioengineering 2024Quote: ... THP-1 cells were treated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Merck Life Science NV) for 24 hours in a T75 culture flask ...
-
bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were settled in a 96-well half area microplate with clear flat bottom (Corning/Merck) at a density of 25,000 cells per well at RT for 20 min ...
-
bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
-
bioRxiv - Cell Biology 2020Quote: Epithelial canine MDCK II (CRL2936) cells were obtained from the ATCC and grown in MEM supplemented with 5% FBS (Merck) at 37°C in an atmosphere of 5% CO2 ...
-
bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...
-
bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
-
bioRxiv - Microbiology 2021Quote: ... and human lung carcinoma (A549) cells (CCL-185) were purchased from ATCC (Manassas, VA USA) and maintained in Dulbecco’s modified Eagle medium (Merck, Germany) supplemented with 10% foetal bovine serum (FB-1365 ...
-
bioRxiv - Developmental Biology 2021Quote: ... the tissues were finely dissected into tiny pieces and enzymatically digested into single cells by incubating them in a dissociation solution containing 0.125 mg/ml Collagenase (Merck, C9891), 1 U/ml Dispase (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were washed once and then incubated with the appropriate fluorescently labelled secondary antibody (anti-mouse polyvalent Ig-FITC (Merck) or anti-His6 HIS.H8 DyLight 488 ...
-
bioRxiv - Immunology 2020Quote: ... After incubation cells were washed 3 times with PBS containing 0.5% BSA and subsequently fixed 1% (w/v) paraformaldehyde (PFA, Merck Millipore) in PBS ...
-
bioRxiv - Cell Biology 2021Quote: Equal numbers of c-Kit+ bone marrow cells were expanded in the presence of doxycycline for 24 hours and treated with cycloheximide (CHX; 100 µg/mL; Merck) for 8 min at 37 °C before harvesting by centrifugation ...
-
bioRxiv - Developmental Biology 2021Quote: ... The cell pellets were resuspended in PBS+0.5% Triton-X 100 supplemented with Complete™ protease inhibitor cocktail (Merck, USA) and frozen overnight at −80 °C for lysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... BM cells harvested from moribund mice were cytospun and stained with Giemsa’s azur-eosin-methylene blue solution (Merck, cat.no. 109204).
-
bioRxiv - Cancer Biology 2021Quote: ... Apoptotic cell death was analyzed by staining with Annexin V/PI as per the manufacturer’s instructions (APOAF-20TST, Merck Millipore) and analyzed in BD LSR-Fortessa using FACS-Diva software (BD Biosciences) ...
-
bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
-
bioRxiv - Systems Biology 2020Quote: The constructs containing the TCA cycle enzymes fused to the biotin ligase and a FLAG tag were stably integrated in the Flp-In T-REx HeLa cells using the X-tremeGENE 9 DNA Transfection Reagent (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
-
bioRxiv - Cancer Biology 2020Quote: ... MCF7 cells were transduced with lentiviral particles at 75% confluency in the presence of 10 μg/ml polybrene (Merck, Germany). 24h after transduction virus containing medium was replaced with selection medium for the respective expression constructs to start the selection ...
-
bioRxiv - Cell Biology 2021Quote: ... The following day cancer cells were serum-deprived for 24h and then all cell lines were treated either with camptothecin 1-10μM (ref. C9911, CPT; Sigma-Merck, Argentina) or DMSO (ref ...