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Citations for Merck :
1701 - 1750 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells from identical conditions were collected and pooled in 10 ul in calcium-free and magnesium-free HBSS (Merck, H8264) + 50% FBS (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Cell Biology 2023Quote: Proximity ligation assays with Aurora A kinase and MP-GAP were performed in HeLa cells using mouse anti-Aurora A Kinase antibody (1:500, A1231 Merck), rabbit anti-MP-GAP antibody (1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Immunology 2023Quote: CORA receptors were transduced into a previously described Jurkat-based reporter cell line (12) by addition of lentiviral particles in a MOI of 1 and 5 μg/mL Polybrene (Merck) using spinoculation ...
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bioRxiv - Neuroscience 2023Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... GFP-coding or GFP/Flagtagged nanobody constructs optimized for mammalian cell expression were transfected using the GeneJuice transfection reagent (Merck). 24 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... Cells were then acquired on the flow cytometer for ~25 seconds before the addition of 1 μg/mL ionomycin (Merck), and calcium flux was measured for ~1.5 mins.
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bioRxiv - Developmental Biology 2023Quote: ... 2-cell stage embryos were similarly recovered from hybrid superovulated females (using 5IU of PMSG and 5IU of hCG; Merck), generated after crossing BDF1 male and CD1 females (each strain obtained from Anlab ...
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bioRxiv - Cell Biology 2023Quote: ... Ninety μl of cell lysates were mixed with 10 μl of lysis buffer containing 0.1 μl benzonase (Merck Millipore, 70664) and further incubated on ice for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and the resulting gel disc was mounted in a live imaging chamber (Aireka Cells) with low-melt agarose (A7431, Merck). The chamber was filled with ddH2O to prevent sample shrinkage and drying ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Molecular Biology 2023Quote: ... At 48 hours of transfection (If indicated) the cells were treated with the following conditions before harvesting at indicated times: 2mM of H2O2 (MERCK) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibody was washed again three times with PBS and the cell nuclei were then counter stained with DAPI (Merck). Epifluorescence microscopy was done in an inverted Olympus IX70 and Confocal microscopy was done in a Zeiss Axioscope 2 Plus microscope equipped with a Bio-Rad Radiance 2100 laser scanning system and Lasersharp 2000 imaging software and a Leica SP8 inverted focus microscope and analyzed with the Leica Application Suite (Las ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Immunology 2023Quote: ... Antibody-stained cells were subsequently probed with Duolink In Situ PLA Probe anti-mouse PLUS and anti-mouse MINUS probes (Sigma/Merck). Next ...
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bioRxiv - Cell Biology 2023Quote: ... after centrifugation the cell pellet was resuspended in 0.1 M HCL solution containing 0.5 mg/ml of the protease pepsin (Merck Life science) and left for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Neuroscience 2023Quote: ... The next day cells were split for selection on 400 μg/ml collagen-IV and 100 μg/ml fibronectin-coated (both from Merck) Transwell inserts (Corning ...
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bioRxiv - Cell Biology 2024Quote: ... the cell pellet was resuspended in 400 μl of 40 μg/ml ultra-pure digitonin (Merck, CAS 11024-24-1) in vPBS and incubated (25 min ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell culture supernatants from 4950T or 170162T cells were concentrated 10x using Amicon centrifugal filtration units with 100 kDa cutoff filter (Merck). Concentrated supernatant or glycodelin A isolated from amniotic fluid was added to the lectin coupled beads and incubated overnight on an overhead shaker at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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bioRxiv - Developmental Biology 2023Quote: Upper and lower arms were harvested from 4 cm double reporter axolotls (Alx4:mCherry_Hand2:EGFP) and dissociated into single cell suspensions using Liberase TM enzyme (Merck 05401127001) as described in 49 ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells pellet was resuspended with a freezing medium composed of 90% KSR and 10% of Dimethyl Sulfoxide (DMSO, #D2438, Merck). Cell vials were stored in a liquid nitrogen tank.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cytospins were prepared by spinning 30.000 cells per slide and stained with May-Grünwald-Giemsa staining (Merck KGaA, Darmstadt, Germany). 2*200 cells were identified by morphology and counted at 20x magnification (Olympus BX51) ...
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... freeze-thaw cycles were implemented to lyse cells and then with benzonase (50 U/mL; Merck Millipore, Burlington, MA, USA) for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: 1×106 of splenocytes or lymph node cells or primary B cells were added to 24-well plates and were stimulated with the TLR4 agonist LPS (L6529, Merck), the TLR7 agonist Imiquimod (HY-B0180 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4°C with primary antibodies (α-TOM20 Santa Cruz # sc-11415; α-STIM1 Merck WH0006786M1) diluted (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were starved (0% serum) for 24h and then treated for 96h with 5mM Metformin (Merck, cat. nr M0605000) or 2ng/ml TGF-β1 (Peprotech cat nr 10021) ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Molecular Biology 2024Quote: ... Cumulus cells were removed by briefly culturing the zygotes in potassium simplex optimization medium (KSOM) (catalog no. MR-101-D, Merck) supplemented with 0.3 µg.µL-1 hyaluronidase (catalog no ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were plated per well and were further cultured in the presence or absence of 5 µM oligomycin A (Merck) at 37 °C in a humidified chamber containing 5% CO2 and 1% O2 for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293 were seeded at 500,000 cells/mL concentration in 25 cm2 flasks and transfected after 24h with plasmid encoding nirFAST-P2A-EGFP using GeneJuice (Merck) according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... The explants were allowed to attach to the feeder layer before epithelial cell medium (ECM) containing Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DNEM-F12, #D8062, Merck) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated in T cell medium for 30 min at 37°C without any metabolic inhibitors or in presence of 1 μM oligomycin (Merck), 100 mM 2DG (Merck) ...
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bioRxiv - Genomics 2023Quote: ... Cell lysis was achieved by pre-incubating bacterial colonies in Phosphate-buffered saline (PBS) containing 100 μg/mL lysostaphin (Merck) at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Immunology 2024Quote: ... The lentivirus particles were centrifuged onto the cells for 90min at 500g at 30°C in the presence of 4μg/ml polybrene (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Cell Biology 2020Quote: Flp-In™T-REx™293 and HEK293 cell lines were grown under standard conditions (37°C, 5% CO2) in Dulbecco’s modified Eagle’s medium (Merck-Sigma-Aldrich, D5796). For Flp-In™T-REx™293 the medium was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...