Labshake search
Citations for Merck :
1351 - 1400 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the GenElute™ Bacterial Genomic DNA kit (Merck) as per manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were incubated with filipin complex (Merck, cat #F-9765; 0.15 mg/ML in D-PBS) for 2 h and after several washes in D-PBS were visualized by fluorescence microscopy ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with primary antibodies [anti-FLAG antibody (M2, Merck Millipore/Sigma, 1/300), anti-HA antibody (3F10 ...
-
bioRxiv - Cell Biology 2023Quote: Hela Kyoto or U2OS cells were grown on coverslips coated with Poly-D lysine (Merck, #P7280). Coverslips containing cells were gently washed twice with 1X PBS to remove excess media ...
-
bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 hour with 10 µg/mL Calcofluor White M2R (CFW) (Merck KGaA). Cells were harvested by centrifugation (2,000 ×g ...
-
bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
-
bioRxiv - Molecular Biology 2023Quote: pCold-DDX43 and pCold-DDX6 were transformed into Rosetta 2 (DE3) competent cells (Merck Millipore/Novagen). The cells were cultured at 37°C until the OD600 reached ∼0.6 ...
-
bioRxiv - Cell Biology 2023Quote: Cultured HAEC cells were stained for SA-β-galactosidase according to the manufacturer’s protocol (Merck Millipore). Briefly ...
-
bioRxiv - Developmental Biology 2023Quote: ... Anterior and posterior samples were dissociated into single cell suspensions using Liberase TM enzyme (Merck 5401119001) as described in 49 ...
-
bioRxiv - Molecular Biology 2024Quote: Cultured HAEC cells were stained for SA-β-galactosidase according to the manufacturer’s protocol (Merck Millipore). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in the presence and absence of 1 μg/ml tetracycline (Sigma-Aldrich / Merck) for RNAi induction ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The acquisition of 30000 cells was performed with a flow cytometer (Guava EasyCyte™, Merck Millipore) and data were analyzed using Incyte™ (Millipore).
-
bioRxiv - Cancer Biology 2023Quote: ... B2M negative cells were resuspended in PBS supplemented with 2.5 mg/ml DNase I (DN25, Merck) and 10 % trypan blue solution (T8154 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were counted manually via the BRAND counting chamber Neubauer improved (Sigma Aldrich/Merck, BR717810-1EA) under a 10X objective lens using a Leica DMi inverted microscope ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cells were counted manually via the BRAND counting chamber Neubauer improved (Sigma Aldrich/Merck, BR717810-1EA) under a 10X objective lens using a Leica DMi inverted microscope ...
-
bioRxiv - Cell Biology 2023Quote: ... THP-1 cells were grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Merck, R8758-500ML) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... HeLaM and HEK-293T cells were grown in Dulbeccos modified Eagles medium (DMEM) (Merck, D6429-500ML) supplemented with 10% Foetal bovine serum (FBS ...
-
Regulatory T cells control type 1-driven immunopathology restraining GM-CSF-producing helper T cellsbioRxiv - Immunology 2024Quote: Treg cell-depletion was achieved by intraperitoneal (IP) injection 500 ng diphtheria toxin (DT) (Merck-Millipore) dissolved in of 150 µl of PBS on days 0 and 1 ...
-
bioRxiv - Immunology 2024Quote: ... the cell lysates were incubated with 15 μl of SA beads (Invitrogen, 65601; Merck Millipore, 69203) for 6 hours at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... The stained cells were analyzed using the ImageStream X Mark II Imaging flow cytometer (Merck Millipore) with an X60/0.9 objective ...
-
bioRxiv - Immunology 2024Quote: ... Cell suspensions were incubated with directly conjugated fluorescent antibodies in PBS/1% (w/v) BSA (Merck)/0.05% (v/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were seeded and incubated as described above and supplemented with 50 nM RSL3 (Merck, #SML2234) and 10% FBS ...
-
bioRxiv - Microbiology 2020Quote: ... cells were passed through a 0.1 micron polycarbonate filter (Whatman nucleopore track-etch membrane, Merck, Southampton, UK) directly following the initial fixation and then processed as described above.
-
bioRxiv - Microbiology 2020Quote: ... Supernatant was discarded and cell pellet resuspended in 1.8 ml ice-cold 50 mM CaCL2 (Merck, 1.02083.0250), incubated on ice for 20 min and centrifuged for 2 min at 5000xg at 0°C ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were fixed with 4% paraformaldehyde (Intron, Seongnam, Republic of Korea) and 100% methanol (Merck, Darmstadt, Germany). The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon ...
-
bioRxiv - Developmental Biology 2020Quote: ... the number of viable cells was counted using the Muse Count & Viability Assay Kit (Merck Millipore MCH100102) and run on the Muse cell analyser (Merck Millipore) ...
-
bioRxiv - Immunology 2021Quote: ... Inhibition of the TLR-4 pathway was achieved by treating cells with 2 µM TAK-242 (Merck) for 6 hours before adding particles ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... the embedded cells were lysed in a lysis buffer containing 2.5M NaCl (Merck Milipore, Burlington, MA, USA), 1 mM Na2EDTA (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2019Quote: ... These cell lines were routinely tested for mycoplasma infection and treated with BM-Cyclin (Merck, Burlington, USA) to eliminate mycoplasma ...
-
bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney (293T) cells were maintained in the Dulbecco’s Modified Eagle Medium (D6046, Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FB-1365 ...
-
bioRxiv - Neuroscience 2020Quote: ... cells were lysed and subjected to subcellular fractionation using a ProteoExtract Subcellular Proteome Extraction Kit (Merck, #539790) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The Bin1b-SH3 construct was transformed into Eschericia coli RosettaTM 2 (DE3) competent cells (Novagen, Merck, 71403) by heat shock ...
-
bioRxiv - Systems Biology 2020Quote: ... The cells were allowed to attach for 24 hours and then tetracycline (0.13 μg/mL, Merck, T7660) was added in the medium of the stable cell lines for the induction of protein expression ...
-
bioRxiv - Microbiology 2021Quote: Yeast cells were digested at 90°C for 4 hours in 65% (w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.5% (v/v ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 48 h after transfection in 2x Laemmli buffer containing 200 mM dithiothreitol (DTT, Merck). Lysates were sonicated and separated by SDS-PAGE/immunoblotting using 7,5 % gels ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were treated for 40 hours with the pan-FGFR inhibitor PD173074 [100nM] (Calbiochem, Merck Eurolab, France). Control cells were treated with DMSO vehicle diluted proportionally to the inhibitor ...
-
bioRxiv - Cell Biology 2021Quote: ... The cells were maintained in 293T medium composed of DMEM high glucose (4.5 g/l; Merck, NJ) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2021Quote: The cells were grown in an 8-well chamber slide (Millicell EX slide, Merck Millipore, Burlington, MA) to early confluency ...
-
bioRxiv - Cell Biology 2022Quote: KELLY cells were treated with 100 μM 5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB, Merck, D1916-10MG) for 3 h at 37°C ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with PBS and blocked with 5% normal donkey serum (NDS) (Merck-Millipore), 0.1% Triton X-100 in PBS for 1 h at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: ... and one of the following primary antibodies: mouse anti-GAD67 for staining GABAergic cells (Merck & Co., MAB5406), goat anti-Substance P for staining D1-containing cells (Santa Cruz ...
-
bioRxiv - Cancer Biology 2022Quote: ... 600,000 cells were lysed using lysis buffer containing 50% of TruPAGE™ LDS Sample Buffer (PCG3009, Merck) and 20% 2-mercaptoethanol ...
-
bioRxiv - Cell Biology 2022Quote: ... at a density of 25×103 cells/cm2 and cultured in EndoGro medium (Merck Millipore, Darmstadt, Germany). TEER was measured daily and experiments were performed when it reaches over 30 Ωcm2 (5-7 days after seeding) ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were washed another 2 times and permeabilised with 0.1% Triton™ X-100 (Merck, UK) diluted in PBS and incubated at room temperature for 5 minutes ...
-
bioRxiv - Cell Biology 2022Quote: The cells used in this study were adherent and cultured in Corning® T75 flasks (Merck, UK) at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... exponentially growing yeast cells (OD600 0.4-0.6) were loaded in commercial microfluidic chips (CellASIC ONIX2, Merck Millipore) as previously described 2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were grown for 48h in serum-free ESGRO Complete Clonal Grade Medium (Merck, Cat# SF001- B) supplemented with 1000 U/ml LIF ...
-
bioRxiv - Microbiology 2021Quote: ... cells were incubated in the presence of 100 μM allylthiourea (ATU; ≥98% purity, Merck KGaA, Darmstadt, Germany), which has been shown to inhibit comammox Nitrospira previously (2) ...
-
bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed before fixation by treating cells with Tyrode’s acidic solution (Merck, T1788-100ML) as described previously (52) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Resistant cells were sorted after TROP2 induction with DOX (final concentration 2 µg/ml; purchased from Merck) by surface labeling with TROP2-specific antibodies (see further).