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Citations for Merck :
1451 - 1500 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The stained cells were analyzed using the ImageStream X Mark II Imaging flow cytometer (Merck Millipore, USA) with an X60/0.9 objective ...
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bioRxiv - Genomics 2024Quote: ... then washed twice in fluorescence-activated cell sorting (FACS) buffer (PBS supplemented with 0.5% BSA (Merck, 10735086001) and 2mM EDTA (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed once with DPBS and stained with 1 μg/ml Hoechst 33342 (Merck, Zug, CH) in a Live Cell Imaging Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cells were harvested for FACS analysis (excitation 488 nm, emission 690/50 nm; Guava easyCyte Flow Cytometer, Merck). Increased red fluorescence has been correlated with the formation of mitochondrial reactive oxygen species (ROS) ...
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bioRxiv - Molecular Biology 2021Quote: Transfected HEK293T cells were treated for 16 h with DMSO or with 10 µM Cyclopiazonic acid (CPA, Merck) before collection.
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The number of cells in each sample was counted using a Guava easyCyte 8HT flow cytometer (Merck, Germany). To describe and compare the effect of each chelator ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Genomics 2019Quote: The populations were enriched in metaphasic cells by 2 h of treatment with 200 nM nocodazole (Merck, M1404) prior to cell recovery ...
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bioRxiv - Cell Biology 2021Quote: ... After 24 h cells were stained for 30 min at 37 °C with NAO dye (200 nM, Merck), washed ...
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bioRxiv - Biophysics 2021Quote: HaCaT mCherry-Histone H2B cells were seeded in 96-well glass bottom plates (Greiner Sensoplate (M4187-16EA, Merck)) at 75 000 cells per well ...
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bioRxiv - Bioengineering 2021Quote: INS-1 cells were stained with red fluorescent membrane-inserting dye PKH-26 (Lot# SLBW0232, Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Each medium collection was concentrated/buffer exchanged to PBS (1X, pH 7.4) using an Amicon stirred cell (Merck) with a 3 kDa molecular weight cut-off membrane ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with 250 μl of PBS and analysed via flow cytometry (Guava easyCyte 5HT; Merck group). 5000 events were recorded per strain and condition using InCyte software (3.1).
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 2 twice with PBS and were permeabilized with PBS–0.06%-Triton X-100 (Merck, USA) for 10 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: Total protein was extracted from the cells 48 h post-transfection by complete lysis-M buffer (Merck, USA). Twenty-five mg of total protein were loaded per well in a 12% SDS PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Microbiology 2019Quote: DF1 cells were seeded into a Chambered #1.0 Borosilicate Coverglass slide (Nunc, Lab-Tek, Sigma-Aldrich, Merck, UK) at a density of 8 × 104 per well and cultured in 1ml of DMEM supplemented with 10% hiFBS ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: Extraction of RNA from cell lines/strains was carried out using TRI Reagent (Merck Life Sciences, Dorset UK) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 16.6 μl/10,000 cells) encoding for ZsGreen transcription in a complex medium containing 5μg/ml polybrene (Merck Millipore). Using qPCR ...
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bioRxiv - Cell Biology 2022Quote: ... The cells at 13 days and 20 days after electroporation were treated with doxycycline (1 µg/mL, Merck) for 24 hours and subjected to microscopic and flow cytometric analyses ...
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bioRxiv - Cell Biology 2022Quote: ... Cells (8 × 104) were seeded on fibronectin (bovine, 0.1 mg/mL) coated Millicell EZ SLIDE 8-Well (Merck) in 300 µL media/well (RPMI + 10% FCS + 1% ultraglutamine + 1% Pen/Strep + 1% HEPES + 1% Na-pyruvate + 1% MEM NEAA + 0.1% mercaptoethanol).
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2021Quote: ... Mononuclear cells were isolated from cord blood fractions by density centrifugation using Ficoll (Biocoll, Merck Millipore, Burlington, Massachusetts). Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec ...
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bioRxiv - Biophysics 2020Quote: ... Pseudotyped GFP expressing coronaviruses were harvested from the cell supernatant 24 hpi and concentrated using Amicon columns (Merck). Then the viruses were titrated in Vero E6 cells and stored at −80 °C ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were subjected to SDS-PAGE and transferred onto polyvinylidene difluoride membranes (Merck Millipore; Darmstadt, Germany). After blocking the membranes with 5% skim milk (Morinaga ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for one hour at room temperature with a mouse monoclonal antibody against γH2AX (Merck) diluted in PBS/SVF (1/1000 ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed using 4% paraformaldehyde and stained with antibodies against ZO-1 (rabbit polyclonal, #617300, Merck-Millipore) and Nestin (mouse monoclonal ...
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bioRxiv - Cell Biology 2019Quote: ... cells released from double thymidine arrest were incubated 4 hrs later with CDK1 inhibitor RO-3306 (Merck millipore) at 9µM concentration for 4 hrs ...
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bioRxiv - Immunology 2019Quote: ... Cells were interferon stimulated by the addition of 100 IU/ml recombinant interferon-β (IFN-β; Merck, 407318) to the growth media for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The human bronchial epithelial cell lines Calu-3 and the Vero E6 cells were cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with 10% standardized fetal bovine serum (FBS Superior; Merck), 2 mM L-glutamine ...
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bioRxiv - Microbiology 2021Quote: ... The human macrophage-like cell line U937 (ATCC no. CRL-1593.2) was cultured in RPMI-1640 medium (Merck) supplemented with 10% iFCS ...
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bioRxiv - Microbiology 2020Quote: ... virus inoculum was removed from each well and cells were overlaid with 300 μL of 0.6% carboxymethylcellulose (Merck) diluted in DMEM supplemented with 2% FBSi ...
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bioRxiv - Immunology 2020Quote: ... PBMC or primary T cells were stimulated with 20 ng/mL Phorbol 12-Myristate 13 Acetate (PMA, Merck), 300 ng/mL ionomycin (Merck) ...
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bioRxiv - Cell Biology 2021Quote: Cells were seeded either on Geltrex coated cover slips or in Millicell® EZ SLIDES (#PEZGS0816, Merck Millipore). Cells were fixed with 4% (vol/vol ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were incubated at room temperature for 20 minutes and analyzed with Muse Cell Analyzer (Merck, Darmstadt, Germany) with 5000 events per sample ...
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bioRxiv - Biochemistry 2021Quote: Full length 6xHIS-Faf1 was expressed from pET28a-Faf1 in Rosetta (DE3) pLysS Competent Cells (Novagen, Merck Millipore) bacteria as above ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were harvested in 4°C RIPA buffer with protease inhibitor (cOmplete, EDTAfree Protease Inhibitor Cocktail, Merck) and homogenised by sonication for 30 sec ...
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bioRxiv - Microbiology 2021Quote: ... The cell suspension was collected by centrifugation and transferred to an activated (by elution of 1mL CH3CN (Merck)) and equilibrated (by elution of 1mL MeOH/H2O (80/20 ...
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bioRxiv - Immunology 2020Quote: ... Primed neutrophils were obtained by treating the cells for 25 mins with 10 μM cytochalasin B (Merck, #C6762) and 100 nM N-Formylmethionyl-leucyl-phenylalanine (fMLF ...
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bioRxiv - Cell Biology 2021Quote: Human hTERT RPE-1 cells (ATCC, CRL-4000) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM/F12, Merck) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2021Quote: ... or 2500 cells/well depending on PARPi dose in 5 ml of medium supplemented with 1.5% methylcellulose (Merck) and the indicated concentrations of Olaparib ...
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bioRxiv - Cell Biology 2022Quote: Double thymidine block (DTB) – exponentially growing cells were treated with 2.5 mM thymidine (Merck Life Science Ltd 6060) for 16 h to arrest them at G1/early S-phase ...
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bioRxiv - Bioengineering 2022Quote: ... the cells were stained with 1 mL 10% Giemsa’s azur eosin methylene blue solution (Merck KgaA, Darmstadt, Germany) in milli-Q water for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... expanding cells were transferred to erythroid differentiation medium (Iscove’s medium with stable glutamine [Merck] containing 3% (v/v) AB serum [Merck] ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Cancer Biology 2022Quote: Xanthine oxidase activity was measured in 4T1 cells cultured in hypoxia using the xanthine oxidase activity kit (Merck) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... before transduction of TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and fresh media added ...