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Citations for Merck :
1401 - 1450 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... allowing the interaction to be observed as PLA foci within the cells (Adopted from Duolink PLA, Merck). PLA was performed on S2 cells that were transfected with the following plasmids using Effectene Transfection Reagent (QIAGEN) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated for 36 hours in the presence or absence of PU-WS13 (Merck Millipore). S ...
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bioRxiv - Microbiology 2020Quote: ... the end-point cell lysis staining assay consisted of adding Neutral Red dye (Merck KGaA, Darmstadt, DE) to cell monolayers ...
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bioRxiv - Microbiology 2021Quote: ... According to the manufacturer’s manual cells were loaded into a CellASIC-microfluidic plate type B04A (Merck Milipore) and mounted on a Delta Vision Elite microscope (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Calu-3 cells were transduced by addition of lentiviral supernatants containing 8 μg/ml polybrene (Merck Darmstadt). 48 hours after transduction ...
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bioRxiv - Neuroscience 2019Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Genetics 2020Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... the cells at a density of 5×105 /ml were supplemented with 2% dimethyl sulfoxide (DMSO; Merck) and the culturing was continued for another 24 to 72 hr ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was cleared of cell debris by filtering through a 0.45µm filter (Corning, Merck, Darmstadt, Germany) and mixed with 1x PEG solution (SBI System Bioscience ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... plasmid coding for 6x-His-Cas9 were transformed in Rosetta DE3 Novagen competent cells (Merck Millipore, USA). Protein production was induced using autoinduction medium (Formedium ...
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bioRxiv - Neuroscience 2021Quote: ... After 24h cells were lysed in RIPA buffer containing 10μg/ml protease and phosphatase inhibitor cocktail (Merck). Lysates were cleared by centrifugation and stored at −20°C until use.
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Immunology 2021Quote: Madin–Darby canine kidney (MDCK) cells were cultured in Eagle’s minimum essential medium (MEM, Merck, Feltham, UK) supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS ...
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bioRxiv - Immunology 2020Quote: ... the cells were further cultured for 4 days in the same conditions plus Dexamethasone (1 μM; MERCK). IDCs were equally generated in 4 days ...
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bioRxiv - Bioengineering 2022Quote: Unfixed U2OS cells were incubated in a prewarmed solution of 0.2% Saponin (#47036, Sigma-Aldrich now Merck) in Cytoskeleton Buffer (CB ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA was extracted from cells using the GenElute mammalian Total RNA miniprep extraction kit (Merck, RTN70-1KT) and was reverse transcribed to synthesise cDNA using High Capacity cDNA Reverse Transcription Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Recipient cells were incubated for eight hours with lentivirus and selected three days later with puromycin (Merck) or G418 (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... cells were quickly but gently resuspended in six-well coated plates with Synthemax (Sigma-Aldrich; Merck KGaA) in MtesR-plus medium supplemented with CloneR (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and seeded onto coverslips that were coated with 25 µg/ml laminin (Merck, #11243217001) in laminin coating buffer (50mM TRIS ...
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Immunology 2022Quote: Cell extracts (1 mg protein) were treated for 15 min at ambient temperature with benzonase (Merck-Millipore) and pre-cleared by incubation for 30 min at 4°C with 20 µl of protein A/G-Sepharose beads ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... cells were incubated with an IF-optimized EMRE antibody (anti-SMDT1/C22orf32/EMRE; #HPA060340, 1:250; Merck), washed 3 times in PBS ...
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bioRxiv - Immunology 2022Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE-171440-02, Merck), collected from the interphase ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed and permeabilized with a 3:1 mixture of methanol (Klinipath)-glacial acetic acid (Merck) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... Transfections were carried out in 6 well plates with cells at 50–70% confluency using GeneJuice (Merck) with 1 μg of total plasmid DNA per well ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cell extracts were concentrated using Amicon Ultra-15 3 kDa cutoff (Merck Millipore, Burlington, MA, USA). The obtained cell extract was flash-frozen in liquid nitrogen and preserved at −80 °C until further use.
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bioRxiv - Molecular Biology 2022Quote: ... Expression of gene of interest was induced by treating cells with 1 μg/ml doxycycline (Merck, D9891) for 72 hours and confirmed by western blotting.
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bioRxiv - Molecular Biology 2022Quote: Cells pellets were resuspended in 200μl lysis buffer (8M urea, 50mM Tris pH 7.5, protease inhibitors (Merck)) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed with 3.7% formalin and methanol and stained with Giemsa and May-Grünwald (Merck), as previously described (68) ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 107 cells were lysed in 200 μL lysis buffer [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... cells were twice washed with PBS and permeabilized with 0.1% TX-100 (in PBS, Merck Life science). Cells were washed an additional 2 times ...
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bioRxiv - Microbiology 2023Quote: ... and VeroE620 cells were maintained in Dulbecco’s Modified Eagle’s High Glucose Medium (DMEM, Merck, Cat. no. D6546), 1% L-glutamine (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... pps1Δ cells were freshly grown on YES medium plate supplemented with 0.4 mM ethanolamine (Merck, 398136-25ML) before liquid culture ...
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bioRxiv - Cell Biology 2023Quote: ... The cell proliferation assay was done using the standard MTT assay (Sigma-Aldrich/Merck, Catalog No. 11465007001). MTT assay was performed on each well following the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with primary antibodies against puromycin (mouse monoclonal 1:500, Merck), Par3 (rabbit polyclonal 1:1000 ...
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bioRxiv - Immunology 2023Quote: Treated BMDM cells were lysed with RIPA buffer (supplied with cOmplete™ Protease Inhibitor Cocktail, Merck#11697498001), and protein concentration was determined by Bradford assay ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated overnight with 30 μL ChIP-grade Protein G agarose beads (Merck Millipore, Germany) and 10 µg DNMT1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then cultured in G-IVF PLUS (Vitrolife, #10136) under mineral oil (Merck, # 8042-47-5) at 37°C in 5% O2 ...
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Ozone mediates tumor-selective cell death caused by air plasma-activated medium independently of NOxbioRxiv - Cancer Biology 2023Quote: ... The cells were maintained in 10% FBS (Serena Europe Gmbh, Brandenburg, Germany) containing DMEM (Merck, NM, USA) supplemented with 100 U/mL penicillin and 100 μg/mL streptomycin (FBS/DMEM ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Cell Biology 2023Quote: HEK cells were grown in an 8-well chamber slide (Millicell EZ slide, Merck-Millipore, Burlington, MA) to early confluence ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and fixed for at least 3 h with 70% ethanol (Merck, 100983). Cells were washed with PBS and analyzed by using the Cell Cycle Kit (Luminex Corporation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell death was assessed by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; S7100, Merck Millipore). Images were obtained with a Leica DM3000 microscope with a mounted Leica DFC420 camera ...
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bioRxiv - Neuroscience 2023Quote: Cell viability was estimated using a propidium iodide stain (2µg/mL; catalogue # P4864, Merck Life Sciences (UK)) as previously published28.
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS+ and incubated twice (15 min each) with 50 mM MESNA (Merck) in 100 mM Tris ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... cell lysates (500 μg) were subjected to immunoprecipitation with LRP1α (1 μg, Cat#: L2295, Merck, Rahway, NJ), HA (0.2 μg ...
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bioRxiv - Genomics 2024Quote: ... then washed twice in fluorescence-activated cell sorting (FACS) buffer (PBS supplemented with 0.5% BSA (Merck, 10735086001) and 2mM EDTA (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The stained cells were analyzed using the ImageStream X Mark II Imaging flow cytometer (Merck Millipore, USA) with an X60/0.9 objective ...