Labshake search
Citations for Merck :
1551 - 1600 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Counterstaining of nucleus was performed using Mayer’s hemalum solution and slides were mounted with Kaiser’s glycerol gelatin (the Kit and all reagents were obtained from Merck Millipore). Images were acquired as described above.
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Microbiology 2021Quote: ... containing a knockout of DNA Ligase 4) 44 that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT; Merck). The transformed cells were plated on minimal synthetic defined (SD ...
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bioRxiv - Plant Biology 2022Quote: ... obtained by grinding in liquid nitrogen and using a SpectrumTM Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, DE) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Microbiology 2021Quote: Ago2 RIPs were performed on TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck millipore) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of the digested RNA was reverse-transcribed as described above and validated by PCR using the KOD Xtreme HotStart Polymerase Kit (#71975-3; Merck, PCR program ...
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bioRxiv - Developmental Biology 2023Quote: ... The reverse transcription reaction to obtain the cDNA was performed with the MystiCq® microRNA cDNA Synthesis Mix kit (Merck) and amplified by PCR using the following primers (miR-34-5p Fw ...
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bioRxiv - Microbiology 2023Quote: The dissolved inorganic carbon concentration needed for the calculation40 was determined using the CaCO3 Merck Spectroquant kit (No. 1.01758.0001) and the Merck Spectroquant Pharo 100 spectrophotometer (Merck, Schaffhausen, Switzerland).
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Molecular Biology 2022Quote: ... the final peak integration was done with the TraceFinder 4.1 software (Thermo Fischer Scientific) using confirmed retention times of 462 metabolites in-house library developed using library kit MSMLS-1EA (Merck). For further data analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting pellet was retained and subject to extraction following the RED Extract Plant PCR-Kit (Merck KGaA, Darmstadt, Germany) protocol.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Cell Biology 2023Quote: ... TUNEL staining was made according to the manufacturer’s instructions of the apoptosis detection kit in situ red (S7165, Merck-Millipore).
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bioRxiv - Microbiology 2023Quote: ... and peak integration was carried out with TraceFinder 4.1 software (Thermo Fischer Scientific) using confirmed retention times for 463 metabolites standardized with a library kit MSMLS-1EA (Merck). Peak smoothening was adjusted to 7 ...
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bioRxiv - Plant Biology 2024Quote: ... Calibration of the column was performed using the Gel Filtration Markers Kit for Protein Molecular Weights 12,000-200,000 Da (#MWGF200; Sigma-Aldrich/Merck, www.sigmaaldrich.com).
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bioRxiv - Microbiology 2022Quote: ... containing a knockout of DNA Ligase 4) [64] that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT, Merck). The transformed cells were plated on minimal synthetic defined (SD ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was isolated from 100 mg of fresh root tissue sampled from three individual plants (biological replicates) using the Spectrum Plant RNA Purification Kit (Merck). RNA was treated with RNase-free DNase Set (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... a tissue sample (up to 100 mg fresh weight per macroalga) was used for genomic DNA extraction using the GenElute Plant Genomic DNA Miniprep Kit (Merck). Genotyping PCR was performed using Prime-STAR GXL DNA Polymerase (Takara ...
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bioRxiv - Immunology 2024Quote: ... LDH activity was quantified in the cell lysate of untreated cells and in all cell supernatants using the Cytotoxicity Detection Kit (LDH) from Merck, following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 6 x 103 hESCs were seeded on mitotically inactivated MEF feeder in a well of 12-well plates and cultured in hESC culture medium for five days before subjected to alkaline phosphatase staining using Leukocyte Alkaline Phosphatase Kit (Merck) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... and a random RT-PCR amplification was performed using the TransPlex® Complete Whole Transcriptom Amplification Kit WTA2 (Merck Millipore) following the manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... The medium was changed every two days for seven days before detection of alkaline phosphatase-positive colonies (Alkaline Phosphatase detection kit, Merck-Millipore). Alkaline phosphatase-positive colonies were analyzed and quantified using ImageJ analysis software (81).
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bioRxiv - Molecular Biology 2020Quote: ... From the remaining nuclei RNA-IP was then undertaken with the MagnaRIP Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit protocol (17-700, Merck) from step I.3 ...
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bioRxiv - Immunology 2021Quote: ... was used for m6A immunoprecipitation (m6A-IP) using an m6A antibody (Synaptic, 202003) following the standard protocol of the Magna MeRIP m6A Kit (Merck Millipore). RNA was enriched through RNA Clean & Concentration-5 (Zymo Research ...
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bioRxiv - Plant Biology 2022Quote: A hole punch was used to obtain approximately 100 mg of leaf tissue from each sample (15 biological replicates per condition) and the total RNAs were extracted using the Spectrum Plant RNA kit (Merck, Spain). RNAs were quantified using the NanoDrop ND-1000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and carbonic anhydrase (29 kD) from a combination of two kits (GE Healthcare, catalogue No. 28-4038-41, Chicago, Illinois, IL, USA and MERCK, Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) histochemistry was performed using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore), according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 2% paraformaldehyde for 10 min at room temperature and stained with the Hemacolor Rapid Staining Kit (Merck Millipore). Images were collected on BX61 upright microscope (Olympus ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR product was run on an SDS gel and purified using the Agarose Gel DNA extraction kit from Merck (11696505001).
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bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
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bioRxiv - Plant Biology 2022Quote: The m6A enrichment experiment was performed as described previously with minor modifications (Dominissini et al., 2013) and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck) was used following the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed using an Apop Tag Plus Peroxidase In Situ Apoptosis Kit (Merck Millipore) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2024Quote: The presence of T-DNA in transgenic plants was confirmed via extraction of genomic DNA and genotyping by PCR with the REDExtract-N-Amp™ Plant PCR-Kit (Merck, Darmstadt ...
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bioRxiv - Microbiology 2024Quote: The activity of the superoxide dismutase (SOD) enzyme was measured in cell lysates using the SOD Determination Kit (Sigma Merck®), and calculations were carried out following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The viability of cells cultured in monolayers was measured using the Cell Counting Kit-8 (CCK-8, Merck Ref 96992, Switzerland). The CCK-8 assay was performed according to manufacturer’s instructions and as described in[99] ...
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bioRxiv - Neuroscience 2024Quote: ... then labeling was performed for one hour at 37°C using a In Situ Cell Death Detection Kit (Merck, cat. 12156792910). Rod photoreceptors were labeled with anti-RHO 1D4 antibody followed by Alexa Fluor 568 anti-mouse secondary antibody (see Table S3) ...
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bioRxiv - Microbiology 2024Quote: SFPQ and FLAG RIPs were performed in TREx-BCBL-1 RTA cells using EZ-Magna RIP RNA binding Immunoprecipitation Kit (Merck Millipore) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purification steps were analyzed by 12.5% SDS-PAGE and WB with the His-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Germany). Protein concentration was determined using the Bradford method (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: The contents of CS in mouse serum and cells were measured with ELISA kits (Cat. No. MAK057; Sigma-Aldrich, Merck KGaA).
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bioRxiv - Neuroscience 2020Quote: ... and the ultraView Universal Alkaline Phosphatase Red Detection Kit (Ventana Medical Systems) or by manual application of biotinylated secondary antibodies (Merck, RPN1001, RPN1004), peroxidase-conjugated avidin ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Microbiology 2021Quote: ... After each PCR step the amplified regions were separated on agarose gel and purified using Illustra GFX™ PCR DNA and Gel Band Purification Kit (Merck KGaA).
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bioRxiv - Neuroscience 2022Quote: Hippocampal tissues from P40 WT mice were collected and lysed with IP lysis buffer in EZ-Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Merck-Millipore). YTHDF2 (Proteintech ...
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bioRxiv - Immunology 2021Quote: ... After each PCR step the amplified regions were separated on agarose gel and purified using Illustra GFX™ PCR DNA and Gel Band Purification Kit (Merck KGaA). The last oePCR step was performed to amplify the complete SARS-CoV-2S D19 sequence using primers carrying 15bp long 5’ overhangs homologous to the vector backbone ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...