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Citations for Merck :
1201 - 1250 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... Expression of wild-type SLC45A4 and mutants were assessed using western blotting on membrane fractions with an anti-FLAG antibody (Merck F1804) at 5,000x dilution ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was immediately separated from the cells by filtration through a 0.45-µm pore size filter (PVD membrane, Merck Millipore Ltd.) ((adapted from [59]).
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bioRxiv - Molecular Biology 2024Quote: ... The tissue homogenates and cell lysates were equally combined with 8-12% SDS-PAGE and transferred onto the polyvinylidene difluoride membranes (0.45 μm, Merck, Darmstadt, Germany). After washing with 0.05% Tween-20 in the Tris-buffered saline (TBST) ...
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bioRxiv - Molecular Biology 2024Quote: ... of 1 L each were filtered through 0.8 μm Whatman (Cytiva, Germany) Cellulose Nitrate membrane filters (25 mm diameter) using sterile 25 mm Swinnex (Merck Millipore, Germany) Filter Holders and a Vampire Sampler (Bürkle GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... of 1 L each were filtered through 0.8 μm Whatman (Cytiva, Germany) Cellulose Nitrate membrane filters (25 mm diameter) using sterile 25 mm Swinnex (Merck Millipore, Germany) Filter Holders and a Vampire Sampler (Bürkle GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were subsequently separated via SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). To prevent non-specific binding ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR amplicon was digested with DpnI and desalted for 15 min by drop dialysis using a mixed cellulose ester membrane (0.025 µm size, Merck Millipore Ltd) and then used for transformation into the L ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein was separated by sodium dodecyl sulfate-polyacrylamide (SDS) gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (PVDF, Merck Millipore). The membrane was then blocked for 1 h with 5% skimmed milk and incubated with the following primary antibodies overnight followed with horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: ... and Click-iT® EdU (5-ethynyl-2’deoxyuridine) Assay (BCK-EDU488, baseclick GmbH, Merck/Sigma-Aldrich, UK), respectively ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the compounds were tested by use of a MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay (Merck) at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Immunology 2024Quote: ... For reporter gene assays 293FT cells were transiently transfected using a slightly modified polyethylenimine (PEI, Sigma-Aldrich/Merck) transfection method72 ...
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bioRxiv - Genomics 2023Quote: Luminex assays were performed using the Milliplex Human Cytokine/Chemokine Magnetic Bead Panel (HCYTOMAG-60K-41, Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Microbiology 2024Quote: The enzyme assay couples the formation of ADP to NADH oxidation via PK (rabbit muscle, (Merck, Darmstadt, Germany)) and LDH (rabbit muscle ...
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bioRxiv - Pathology 2021Quote: ... KAPA SYBR® FAST kit and qPCR kit were purchased from Merck (Darmstadt, Germany). RNeasy Mini kit and QIAcube were obtained from Qiagen (Venlo ...
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bioRxiv - Biochemistry 2023Quote: ... while an ApopTag Kit (Merck) was used to detect apoptotic cells in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... A total of 30 μg of protein from each sample were subjected to SDS-PAGE electrophoresis and transferred to PVDF membrane (Merck Millipore, USA). Then the membrane was blocked in 5% w/v bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... biofilm samples were rinsed with deionized water and deposited on polycarbonate filters (GTTP Isopore membrane filters, Merck Millipore, pore size 0.2 μm) or on glass slides (for correlative Raman analyses ...
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bioRxiv - Genomics 2020Quote: ... An amount of 35 or 40 μg whole cell lysates or tissue lysates were resolved by 10% SDS-PAGE and transferred onto PVDF membranes (Merck Millipore, Germany). After incubation in 5% non-fat milk for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... groundwater was pumped directly into a sterile chilled Nalgene filtration unit fitted with a 0.22 µm pore size Isopore polycarbonate membrane (Merck Millipore, Darmstadt, Germany). Approximately 10 L of groundwater was filtered each for metagenomic and metaproteomic sample and 1 L was filtered when samples were taken for 16S rRNA gene amplicon analysis only ...
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bioRxiv - Synthetic Biology 2021Quote: ... Then equal amounts of total protein in each condition were resolved by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, ISEQ00010). The PVDF membrane was blocked with 1% (for anti-FLAG antibody ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
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bioRxiv - Microbiology 2021Quote: ... A total of 20 µg of protein from each sample was subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Merck Millipore, USA). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was harvested by centrifugation at 10,000 x g at 4°C for 30 min and filter-sterilized using a 0.45 μm pore size hydrophilic PVDF membrane (Millex®-HV 0.45 μm, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to Immobilon 0.20 μm (LC3, GAPDH) or 0.45 μm (CD9, CD63) pore-sized PVDF membranes (Merck Millipore Ltd., Cork, Ireland) by wet transfer ...
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bioRxiv - Genomics 2021Quote: ... The In-Fusion reactions were dialysed against water in a 6 cm petri-dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, New Jersey) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Axonal fraction and the fraction containing neuronal body with dendrites were isolated by carefully detaching the cellular contents from the inner and outer insert membrane surface with a cell scraper in TAEB buffer (Merck, Darmstadt, Germany) four times ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were then transferred by wet tank-blotting (100 V for 60 min) onto a polyvinylidene difluoride (PVDF) membrane (Merck, Darmstadt, Germany). The membrane was blocked with Roti®-Block (2 h at room temperature ...
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bioRxiv - Immunology 2022Quote: Reduced cell extracts (20 µg of protein) or immunoprecipitates were resolved by SDS-PAGE and transferred to PVDF membranes (Merck-Millipore, #10344661) using a Trans-Blot transfer cell (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... Dust samples were collected by impaction on preconditioned and pre-weighted 25 mm hydrophilic polycarbonate membrane filters with a pore size of 0.8 µm (Merck KGaA, Darmstadt, Germany) mounted in antistatic polypropylene total dust filter cassettes (Pall Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... RNAs were chemically cross-linked to the membrane for 90 minutes at 65°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Merck, Sigma Aldrich). The membrane was pre-hybridized for 30 minutes in Perfect Hyb plus (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubation on ice for 10 min and loaded on SDS-PAGE gel and wet transfer onto PVDF membrane (Merck, Cat. # IPFL00010). Thereafter ...
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bioRxiv - Microbiology 2024Quote: ... for 10 minutes in the dark and then filtered through Nuclepore black polycarbonate membrane filters (0.22 µm pore size, Merck Millipore, Darmstadt, Germany) with negative pressure (≤ 20 kPa) ...
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bioRxiv - Microbiology 2024Quote: ... a volume of 20 mL with an OD720 of 0.6 was withdrawn from the culture grown in the desired conditions and vacuum filtered through a Durapore® 0.45 µm PVDF membrane (Merck Millipore-Ireland). The filter with the biomass was immediately inverted into a petri dish with 1.6 ml of pre-cooled methanol for quenching and incubated at −80°C for an hour with periodic rubbing of the filter against the container to aid cell lysis ...
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bioRxiv - Genomics 2024Quote: ... 500 mL vent-water was passed through a sterile 0.22 μm mixed cellulose ester membrane filter (Merck Life Science Private Limited, India) having a diameter of 47 mm ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was filtrated through a filter paper (Whatman No. 4, Whatman plc, Kent, UK) and a membrane filter (MF-milliporeTM 0.22 μm, Merck KGaA, Darmstadt, Germany). The solution was cleaned up through a Waters Sep-Pak C18 column (Waters ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of protein were separated by 10% SDS-PAGE gels and were blotted onto a high-resolution nitrocellulose (NC) membranes (MERCK, Darmstadt, Germany). Membranes were blocked with 5% Albumin Bovine Fraction V (SERVA ...
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bioRxiv - Cancer Biology 2023Quote: ... The separated proteins were transferred from the gel to the polyvinyl difluoride (PVDF) membrane (Immobilon-P, 0.45 µm, Merck Millipore Ltd, Ireland) at 200 mA for 1.5 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: PrPC was filtered through a 100 kD membrane filter in 10 mM Bis Tris pH 6.5 (Amicon ULTRA 0.5 mL 100K 96PK, UFC510096, Merck Life Science UK Ltd) to remove aggregates and then diluted into reaction buffer (50 mM Na-phosphate ...
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bioRxiv - Biophysics 2024Quote: ... Channel subunits in streptavidin precipitates and total lysates were analysed by Western blotting on 8% sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) gels and transferred onto a polyvinylidene fluoride membrane (Merck, Milan, Italy).
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampi were dissected and sliced into 350 um thick transverse slices with a use of McIlwain tissue chopper and placed on PTFE membranes (Millicell; Merck, Cat# PICMORG50). Cultures ware kept in the air-medium interphase ...