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Citations for Merck :
1151 - 1200 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots of 2 ml of each recipient and donor strains were combined (three mating pairs in total) and filtered through sterile 0.22 µm membranes (Merck Millipore, GSWP02500), which were incubated over TSA plates for 8 days at 22°C ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were transferred to a low fluorescent polyvinylidene difluoride membrane Immobilon-FL (0.45 μm pore size; Merck-Millipore, Czech Republic) by a Mini Trans-Blot® cell system (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... A sequence of coronal slices (400 μm) was cut at the level of the somatosensory cortex and plated on Millicell-CM organotypic culture membrane inserts (Merck Millipore). Slices were cultured in a medium containing 50% Optimem-1 ...
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bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted fractions were concentrated (Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane, EMD Millipore, Merck, Burlington MA, USA) and purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE LS ...
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bioRxiv - Microbiology 2022Quote: ... Proteins in cell lysates were separated by 10% SDS-PAGE and transferred to Immobilon NC membranes (Millipore Merck KGaA, Darmstadt, Germany). The membranes were blocked by incubation with 5% nonfat dry milk in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... protein lysate (30 μg) was loaded onto and run through a 10% SDS-PAGE gel and transferred to a PVDF membrane (Merck, IPVH00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... Antibody preparations for in vivo infusions were micro-filtered (Ultrafree®-CL devices - 0.1 µm PVDF membrane, Merck-Millipore, Darmstadt, Germany), and checked for endotoxins levels using the ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit (GenScript).
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bioRxiv - Cancer Biology 2022Quote: ... 10 ml of medium were filtrated on 0.45 µm PVDF membrane of Millex-HV® filter unit (Cat. No. SLHV R25 LS, MERCK KGaA), 3.5 ml of 4x PEG solution (32% PEG6000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 45-μm polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204 ...
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bioRxiv - Microbiology 2021Quote: ... structured microcosms were set-up in multi-well plates with a 0.22 µm filter membrane at the bottom of the well (Merck, Darmstadt, Germany) and containing 250 mg of sterile glass beads (with diameter of 80 to 120 µm ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole-cell lysate was loaded and separated on a 7.5% polyacrylamide gel and transferred onto a PVDF membrane (Merck Millipore, IPVH07850). Membranes were blocked with PVDF Blocking Reagent for Can Get Signal (Toyobo ...
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bioRxiv - Cell Biology 2022Quote: ... Purified chromatin was separated by SDS-PAGE and transferred to 0.45 μm polyvinylidene difluoride membranes (Immobilon, Millipore, Merck KGaA, Darmstadt, Germany). Blots were incubated with primary antibodies HDAC1 (Cell Signalling # D5C6U ...
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bioRxiv - Immunology 2022Quote: ... removed and faux adherent cells from the lower membrane washed into the bottom well before migrated cells were counted using a Guava EasyCyte 8HT Flow cytometer (Merck Millipore). Data was then presented as the total number of migrated cells or relative to vehicle controls (% control).
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bioRxiv - Immunology 2022Quote: ... resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Laemmli running buffer and transferred to PVDF membranes (Merck-Millipore) in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... and afterwards transferred to a methanol- activated Immobilon-P PVDF (poly-vinylidene difluoride) membrane (catalog#IPCVH00010, Merck Millipore Ltd., Carrigtwohill, Ireland) by tank blotting at 250mA for 90 min in a cold room (4 °C ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Immunology 2024Quote: Net ALI membrane resistance for each sample was determined by measuring the sample resistance with a MillicellERS-2 Voltohmmeter (Merck Millipore) then subtracting the blank sample resistance reading ...
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bioRxiv - Microbiology 2023Quote: ... The elution buffer was further exchanged with Thermo Scientific BupH Tris Buffered Saline (TBS) by using Amicon Ultra-15 Centrifugal Filter Units with Ultracel-10 membrane cutoff (Merck Millipore) and protein concentration was measured with a Qubit 2.0 fluorometer.
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysate was then separated by electrophoresis in a 10% polyacrylamide gel and transferred to a PVDF membrane (Merck Millipore). The membrane was blocked with 5% milk in PBS-T ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Plant Biology 2023Quote: ... The separated total proteins or proteins from two-dimensional gels were subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck, Cat. IPVH00010), hybridized with specific antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE and were blotted onto an Amersham™ Protran® nitrocellulose membrane (Merck KGaA; Darmstadt, Germany) for 1.5 h using const ...
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bioRxiv - Microbiology 2022Quote: ... Three microliters of cell suspension (whole cell) or cell lysate were spotted on an Immobilon-PSQ transfer membrane (Merck Millipore Ltd.) and air dried ...
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bioRxiv - Cell Biology 2023Quote: ... and the trophozoites were resuspended in 500 μl NaPBS/0.05 % saponin (or permeabilization of cell membranes (Sigma-Aldrich, Merck, Taufkirchen, Germany) and incubated for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The indexed PCR products were again normalized by loading 15 µl indexed PCR products onto Sequal Prep TM Normalization plates and were pooled and concentrated using a Amicon® Ultra 0.5 ml centrifugal filter (30K membrane, Merck Millipore). A NanoDrop™ One Microvolume Spectrophotometer (Thermo Scientific™ ...
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bioRxiv - Genomics 2022Quote: ... The NEBuilder reactions were dialyzed against water in a 6 cm Petri dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, NJ) for 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell lysates were separated via SDS-PAGE and transferred to Immobilon-P PVDF membranes (IPVH00010, Merck & Co., Inc., USA). These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 50 μg of proteins were separated by 10% or 15% SDS- polyacrylamide gel (SDS-PAGE) and transferred to a nitrocellulose membrane (Merck, HATF00010). To detect the CXCL16 and CXCL8 secreted in the culture medium ...
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bioRxiv - Neuroscience 2023Quote: ... and the retina was separated from the eyes (see section Retina Isolation) and mounted ganglion-layer up on membrane filter (HAWP04700, Merck, USA). The retina was then secured with slice anchors (SHD-27LH/10 ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the extracted liquid sample was collected by centrifugation through a filtered microcentrifuge tube (cellulose acetate membrane with pore size 0.45 μm; Merck CLS-8162), (x ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto Immobilon®-P PVDF membrane (Merck), followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was filtered through a hydrophilic polytetrafluoroethylene membrane filter (Millex-LG, 0.2 um × 4 mm, Merck Millipore, Darmstadt, Germany) and transferred to a screw-top glass vial ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...