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Citations for Merck :
1251 - 1300 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
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bioRxiv - Immunology 2024Quote: ... And then the proteins were separated on 12.5% SDS-PAGE gels and then transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking with 5% nonfat milk diluted in Tris Buffered Saline with Tween 20 (TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was rinsed with TBS buffer and incubated with the primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) (1:2,500 ...
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bioRxiv - Biophysics 2024Quote: ... 500 ng of BiP were separated via SDS-PAGE on 10 % polyacrylamide gels (homemade) and transferred on Immobilon®-P PVDF membranes (Merck-Millipore) for 1.5 h at 230 mA using a Biometra Fastblot B44 (Analytik Jena ...
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bioRxiv - Cell Biology 2021Quote: Radial 3D invasion assays were performed by embedding glioblastoma spheroids into a 50% Matrigel® solution (Corning®, Merck) (1:1 Matrigel® diluted in spheroids + GBM medium) ...
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bioRxiv - Cell Biology 2022Quote: ... For colony survival assays with BI2536 (Selleck-chemicals), talazoparib (Cat#S7048, SMS-Gruppen Denmark) or CPT (Cat#C9911, Merck), the methylcellulose medium with drug was shaken for more than 1 hour at 4°C before transfer into 6 well plates with 5 ml medium each well.
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bioRxiv - Neuroscience 2022Quote: ... 50 μL/well of detection antibody (anti-FLAG-D2) diluted in Erenna Assay buffer (catalog #02-0474-00; Merck) at the final concentration of 1 μL/mL were added to the assay plate and incubated for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The in situ proximity ligation assay (PLA) in combination with immunofluorescence microscopy was performed using the Duolink Detection (Merck) or the NaveniFlex (Navinci diagnostics ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2024Quote: ... qPCR or EdU proliferation assay) for 72 h in the presence or absence of 200 nM Smoothened agonist (Merck). Full medium changes were performed daily ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Cell Biology 2022Quote: ... the SDS samples were applied onto an Amicon filter unit (MWCO = 10 kDa; Ultracel-10 cellulose membrane, Cat.No; UFC201024, Merck Millipore, Billerica, MA, USA) to trap the proteins ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Microbiology 2020Quote: ... and HA-tagged proteins of samples were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to a PVDF membrane (Merck Millipore, Tokyo, Japan). The membrane was incubated with rabbit anti-N antiserum or mouse anti-GFP antibody ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Cell Biology 2020Quote: ... The pooled fractions were transferred to ice-cold PBS pre-rinsed Amicon Ultra-4 centrifugal filter units with Ultracel-30 membrane (Merck Millipore, Massachusetts, USA), then centrifuged with 3,200 x g at 4 °C for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
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bioRxiv - Cell Biology 2020Quote: ... were separated with a 10% SDS-PAGE resolving gel using the Laemmeli discontinuous buffer system and then transferred to a PVDF transfer membrane (Immobilon-FL, Merck KGaA, Darmstadt, Germany) using a Biorad wet transfer apparatus ...
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bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... Four small insertions were made and the retina was flat mounted on a filter paper annulus (1 mm inner radius; 0.8 µm hydrophilic MCE MF-MilliporeTM membrane filter, Merck Millipore Ltd., Tullagreen, Ireland). The retina was then mounted photoreceptor cell side up on a perforated 60-electrode MEA chip (60pMEA200/30iR-Ti ...
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bioRxiv - Biophysics 2024Quote: ... The phages were then purified with two different protocols: Protocol 1: Phage lysates were filtered through 0.22 µm polysulfone membrane filters (Merck Millipore, Billerica, MA, USA) and were used for subsequent purification and concentration by cross-flow filtration using Sartorius Hollow fiber cartridges ...
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bioRxiv - Microbiology 2023Quote: ... Cell-free extracts were then resolved by SDS-PAGE using 12.5%-15% polyacrylamide gels and subsequently electro-blotted onto a 0.45 μm polyvinylidene difluoride (PVDF) Immobilon®-P membrane (Merck Millipore, Sigma Aldrich) at 100 V for 90 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... and then clarified via passing through Millex® -GP Fast Flow & Low Binding Millipore Express® PES Membrane 0.22 µm syringe filter unit (SLGP033RS, Merck Millipore, USA).
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bioRxiv - Neuroscience 2024Quote: ... Membranes were visualized using Fusion Fx (Vilber Lourmat, Marne-la-Vallée, France) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA). Protein levels were quantified using ImageJ and normalized to the β-actin levels.
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bioRxiv - Plant Biology 2024Quote: ... The seedlings were cut perpendicularly in the upper hypocotyl with an injection needle tip (NN-2613S; TERUMO, Tokyo, Japan) on a membrane filter (HAWP04700; Merck Millipore, Darmstadt, Germany). The obtained scion was connected with the partner rootstock through a 0.4-mm silicon microtube (1-8194-04 ...
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bioRxiv - Synthetic Biology 2024Quote: ... A thin film of excised bronchial tissue from rats was evenly distributed in the space between a pair of semi-penetrating membrane filters (Merck Millipore, 2.0 μm) placed between the donor and acceptor chambers ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of proteins per sample were subjected to SDS-PAGE and transferred to Immobilon-PSQ PVDF transfer membrane (Merck Millipore, Darmstadt, Germany) with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the alkaline phosphatase detection kit (Merck) was used following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... cell counting kit 8 (Merck, 96992) was applied for 2.5 h as recommended by the manufacturer.
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bioRxiv - Synthetic Biology 2024Quote: ... The GenElute Plasmid Miniprep Kit (Merck) was used for plasmid purification ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed and scraped in homemade Radioimmunoprecipitation assay buffer (RIPA) ((50 mM Tris pH 7.4, 150 mM NaCl, 1 % Triton X-100 (Merck, T9284), 2 mM EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... An assay volume of 150 μl was dispensed into a Hellma 10 x 2 mm Suprasil quartz cuvette (Merck #Z802778). For double-stranded substrates ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Biochemistry 2024Quote: ... H2O was removed and ROS production was measured upon addition of a 100-μL assay solution which contained 10 μg/mL horseradish peroxidase (P6782, Merck), 10 mM luminol and the treatment (1 μM SCOOP ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells following incubation with fibrils formed in the absence/presence of gangliosides was determined using a standard MTT cell proliferation assay (Merck).
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bioRxiv - Neuroscience 2022Quote: ... 100 μL/well of the MW1 antibody coupled with magnetic particles (appropriately diluted in Erenna Assay buffer catalog #02-0474-00; Merck), were added to the assay plate ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... PAM (0.1 mg/mL in DMSO) or DMSO were incubated with LCAT (5 μg/mL in assay buffer + 60 μM albumin (Merck)) 1:100 v/v at 37°C gently shaken ...
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bioRxiv - Neuroscience 2024Quote: Samples of 2mm diameter retinal punches were collected from donor eyes (Supplementary Figure 1A) and homogenized on ice using radio-immunoprecipitation assay (RIPA) buffer (#900000064244, Merck) supplied with phosphatase and protease inhibitor (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... then the acid supernatant was transferred to a 30 kDa centrifuge spin filter (Microcon-30 kDa Centrifugal Filter Unit with Ultracel-30 membrane, Merck, Australia; Product ID: MRCF0R030) prior to further centrifugation at 3,700 rpm for approximately 30 minutes until liquid had passed through the filter membrane ...
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bioRxiv - Microbiology 2022Quote: ... and proteins in the lysate were separated in a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Equal amount of lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride membrane (Merck Millipore, Burlington, MA, USA) in 25 mmol/L Tris ...
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bioRxiv - Genetics 2022Quote: ... 50 μg of protein was used for gel electrophoresis in 10-14% SDS-PAGE gels and transferred to PVDF membranes (Merck Millipore, Billerica, MA, USA). After blocking in 5% defatted milk ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were separated through electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel and then transferred onto an Immobilon-P membrane (Merck Millipore, Burlington, MA, USA). After blocking with 5% bovine serum albumin ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Pathology 2022Quote: ... 50 μg of protein was separated via SDS-polyacrylamide gel electrophoresis (SDS–PAGE; Bio–Rad Laboratories, Inc., Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Burlington, MA, USA). Then ...
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bioRxiv - Microbiology 2023Quote: ... approximately 300 µL supernatant was passed through a 0.2 µm nylon membrane filter (AcroPrep™ Filter plate; Pall Corporation, New York, United States; or syringe filters; Merck & Cie KmG, Schaffhausen, Switzerland). Analysis with a sample volume of 40 µL was carried out using a Chromaster HPLC-System (Hitachi ...
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bioRxiv - Cell Biology 2023Quote: ... Gels were run in 1x running buffer and then the proteins were transferred from the gel in ice cold 1x Buffer onto Immobilon®-P Transfer membranes (Merck Millipore, Cat.No. IPVH00005). Transfer membranes were air dried to dehydrate and stored until use ...
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bioRxiv - Cell Biology 2024Quote: ... An aliquot of 30 μL from each sample was subjected to electrophoresis on 8% or 15% polyacrylamide gel for ARAP1 and ADAP1 each and transferred into an Immobilon®-P transfer membrane (Merck Millipore Ltd., Germany). Membranes were blocked with 5% skim milk (Nacalai Tesque ...
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bioRxiv - Neuroscience 2024Quote: ... each sample was separated on 5-20% gradient e-PAGEL mini gels (ATTO Corporation, Tokyo, Japan) and transferred to polyvinylidene fluoride membranes (Merck Millipore, Billerica, MA, USA). The membranes were incubated for 30 min with blocking buffer (Nacalai Tesque) ...