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Citations for Merck :
1101 - 1150 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The eluted aliquots were then desalted with filtered water and concentrated using an ultrafiltration device with a 3 kDa cut-off membrane (Amicon Ultra-0.5 Centrifugal Filter Unit; Merck, UK) to remove any urea and salt residues.
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bioRxiv - Microbiology 2024Quote: ... Proteins in the lysate were then fractionated by SDS-PAGE and transferred to a PVDF membrane (Merck Millipore, Cat#IPVH00010). After blocking with 1% nonfat milk overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) to 3 mg/ml was used for rabbit immunization.
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bioRxiv - Physiology 2024Quote: ... the excess of reagents was eliminated by performing several washing steps with Milli-Q water using 4 mL cellulose membrane centrifugal filters (Amicon, MilliPore, 100 kDa, Merck). All MNP suspensions were sterilised by filtration using 0.22 μm MilliPore® filters before addition to cell cultures ...
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bioRxiv - Neuroscience 2024Quote: ... were plated for neuronal differentiation in wells pre-coated with Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Merck). Cells were plated in NMM and half the media was replaced twice a week ...
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bioRxiv - Microbiology 2023Quote: ... the solution was transferred to a Millipore Amicon Ultra 30 kDa cut off membrane centrifugal filter unit (Merck Millipore, USA) (prewashed twice to remove glycerol ...
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bioRxiv - Developmental Biology 2023Quote: ... all the samples were filtered through a 47 mm polytetrafluoroethylene polymer (PTFE) membrane with a 0.45 μm pore (Merck OmniporeTM) and rinsed three times with filtered ultrapure water ...
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bioRxiv - Developmental Biology 2023Quote: ... all materials and equipment utilized underwent a thorough three times rinsing process using ultrapure water that had been previously filtered using a 0.1 μm membrane (Merck Isopore). Furthermore ...
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bioRxiv - Biochemistry 2023Quote: ... The prepared samples were separated by SDS-polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride (PVDF) membrane (Merck Millipore). The PVDF membrane was blocked in 5% skimmed milk in Tris-buffered saline containing Tween for 1 hour and incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted fractions were concentrated on Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane (EMD Millipore, Merck, USA) and further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... The phosphorylated histidine was detected by Western blotting by then incubating the membrane for 60 min with 1:1.000 anti-N1-phosphohistidine antibody (Merck Millipore) in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (10 µg) were analyzed by SDS-PAGE and transferred to Immobilon-FL PVDF membranes (Merck Millipore) using Pierce™ 1-step transfer buffer (Thermo ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was filtrated using Millex® HV membrane filter units with a pore size of 0.45 µm (Merck Millipore). Fy-4 was subsequently purified combining Ni-NTA affinity chromatography (HisTrap FF column ...
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bioRxiv - Cell Biology 2022Quote: Proteins (either from whole cell lysates or insoluble fraction) were resolved by SDS-PAGE and transferred to PVDF membrane (Merck) by western blotting ...
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bioRxiv - Cell Biology 2022Quote: ... The viral supernatant was collected after 48 h and 72 h and filtered through a 0.45 µm PVDF membrane (Merck Millipore).
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bioRxiv - Microbiology 2022Quote: ... ZIKV was concentrated using an Amicon Ultra-15 Centrifugal Filter Unit with an Ultracel-100 membrane (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Microbiology 2022Quote: ... The proteins were separated in a 10% SDS-polyacrylamide gel and transferred to Immobilon NC membranes (Millipore Merck, Darmstadt, Germany). The membranes were blocked with 5% non-fat dry milk in PBS for 1 h at room temperature and then incubated with primary antibodies (DB 1:2000 ...
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bioRxiv - Cell Biology 2023Quote: Whole liver lysates and lipid droplet-enriched fraction proteins were loaded on 10% SDS-PAGE gels and transferred onto polyvinylidene fluoride membranes (Merck). Membranes were incubated at room temperature for 1 h with blocking buffer (TBS ...
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bioRxiv - Biophysics 2023Quote: ... The lysozyme protein solution was filtered by a 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA). The filtered sample was stored in 1.0 mL aliquots at −45 °C until crystallization experiments were performed ...
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bioRxiv - Developmental Biology 2024Quote: ... of dialyzed supernatant of organoids which was ultra-filtered into approximately 12-24- fold concentration using AMICON ULTRA-15 10KDA ULTRACEL-PL MEMBRANE (Merck), 50 Units/kg rhFVIII ...
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bioRxiv - Biophysics 2024Quote: ... The protein was then concentrated to about 100 μM (Amicon Ultra-15, PLQK Ultracel-PL Membrane, 50 kDa, Merck, Millipore). After size-exclusion chromatography with a Superdex 200 Increase10/300 GL column (GE Healthcare) ...
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bioRxiv - Physiology 2024Quote: ... 20 µg total protein extracts were separated by 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and cut into 2-3 parts ...
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bioRxiv - Neuroscience 2024Quote: Transfected cells were solubilized in gel loading buffer and analyzed through electrophoresis before being transferred to nitrocellulose membranes (Merck Millipore). Immunodetection was achieved with the primary antibodies (1:1000 in blocking solution) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were loaded in 7 or 12% acrylamide gels and then transferred on a polyvinylidene difluoride (PVDF Immobilon membrane, Merck) membrane and incubated in PBS 0.1% Tween supplemented with 5% milk for 1 h to block non-specific binding ...
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bioRxiv - Cell Biology 2024Quote: ... Each lysate containing the same amount of proteins was separated on 8–12% SDS-polyacrylamide gels (PAGE) and electro-transferred to PVDF membranes (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 60 µL were carefully taken off from each ultra-centrifugation tube and transferred into a 0.2 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore), centrifuged for 5 min at 500 xg and 4°C and the flow through was collected in a 96 well polypropylene plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell supernatants from all samples were carefully collected and 230 µL supernatant was transferred into a 0.45 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore) to remove detached cells and cell debris ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-20 µg protein lysate were separated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) using 8-10% gels and transferred to Amersham Protran 0.45 µm nitrocellulose membranes (Merck, #GE10600012) by wet blotting using the Mini-Protean Vertical Electrophoresis system (Bio- Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated at 95 °C for 5 min before loading onto Bis-Tris gels and wet transfer to a PVDF membrane (Merck). The membranes were then blocked with 5% BSA in TBS-T for 1 h and incubated with the appropriate primary antibodies overnight at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cell lysates were transferred to a 0.45 µm 96-well filter-plate (Durapore, low protein-binding PVDF membrane, Merck Millipore), centrifuged for 5 min at 500 xg and 4°C to remove aggregates and collected in a 96 well polypropylene plate ...
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bioRxiv - Cell Biology 2020Quote: ... the Akt/mTOR phosphoprotein magnetic bead panel 11-plex 96-well plate assay (Merck Millipore, Darmstadt, Germany). This assay uses paramagnetic beads (6.5 μm diameter ...
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bioRxiv - Bioengineering 2022Quote: ... the activity assay of immobilised GalT consisted of 0.16 mM UDP Gal (Merck, 16mM GlcNAc (Sigma-Aldrich), 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: Stress assays (wounding or uraemic serum addition) were performed in Corning® 6-well plates (Merck, UK). The HDF ...
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bioRxiv - Microbiology 2020Quote: ... the end-point cell lysis staining assay consisted of adding Neutral Red dye (Merck KGaA, Darmstadt, DE) to cell monolayers ...
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bioRxiv - Cancer Biology 2021Quote: Cell proliferation was assessed by 3-(4,5-1,2methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (Merck Millipore). K562 cells (5,000/well ...
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bioRxiv - Genetics 2022Quote: Whole-cell protein lysates were extracted in modified radioimmunoprecipitation assay buffer supplemented with protease inhibitors (#5892970001, Merck) and phosphatase inhibitor cocktail (#4906837001 ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Cell Biology 2024Quote: The EMD MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Pane A – Immunology Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Respective ELISA kits (insulin, leptin, IL-6 – MilliPlex Map Mouse Adipokine kit, Merck, Germany ...
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bioRxiv - Microbiology 2020Quote: ... Fifty µg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). The phosphorylated fractions of the MAP kinase ...
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bioRxiv - Microbiology 2020Quote: ... were percussed until the amoebae detached and 1mL of the culture media was filtered through a 3 µm cellulose acetate membrane (Merck, Germany) to retain the A ...
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Bioengineering 2021Quote: Unbound DNA was removed from DNA-SWCNT samples prepared by direct sonication and MeOH-assisted surfactant exchange by rinsing with Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck), as detailed above ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6x Laemmli buffer were subjected to standard SDS-PAGE (10%) prior to being electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Ponceau S staining (Merck ...