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Citations for Merck :
1301 - 1350 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Equal amounts of protein were then separated through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (Merck Millipore Ltd., Tullagreen, Ireland). Membranes were blocked in 5% nonfat dry milk in 1× TBST for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: Proteins in the lysate were separated on a denaturing polyacrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (Merck Millipore, Burlington, MA, USA). The membrane was incubated with 5 % skim milk (BD Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins in 65 μl of conditioned media and cell lysates per lane were separated on 6% polyacrylamide gels using SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred on a poly-vinylidene difluoride (PVDF) membrane (Immobilon-FL, Merck Millipore Ltd., Burlington, MA) using the MiniProteas Blotting Module (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled fractions were concentrated to 20 mg mL-1 using a 10-kDa cut off filtration membrane (Cat. UFC501096, Merck Millipore, Burlington, MA, USA). Sitting drop crystallization trials were set up by depositing 150 nL of the precipitant solution on top of 150 nL of protein with 70 μL of the reservoir using a Crystal Gryphon LCP robot (Art Robbins Instruments) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2958 bp) was purified using commercial DNA purification kits (GenElute™ HP Plasmid Miniprep kit, Merck, #NA0160), according to standard procedures ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNFα were measured in serum samples using MILLIPLEX MAP Porcine Cytokine and Chemokine Magnetic Bead Panel - Immunology Multiplex Assay (Merck Millipore) following the manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine levels produced by PCLS after 2 dpi were assessed in a Multiplex assay in supernatants (dilution 1:2) with MILLIPLEX® Bovine Cytokine/Chemokine Panel 1 (BCYT1-33K-PX15, Merck) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The rest of the supernatant for each sample is used to measure the total protein level of the sample with a Bradford assay (Merck # B6916), following reagent protocol ...
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bioRxiv - Microbiology 2023Quote: ... The PLA assay was performed according to the manufacturer’s instructions using the In Situ Detection Reagents Red (Catalogue Number: DUO92008, Merck, Darmstadt, Germany).
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bioRxiv - Bioengineering 2023Quote: An immunofluorescence assay was performed in cryosections of rd10 animals using the following antibodies: rabbit polyclonal cone arrestin (1/5000, Merck Millipore), mouse monoclonal rhodopsin (1/1000 ...
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bioRxiv - Microbiology 2024Quote: ... a red tetrazolium salt that turns yellow when cleaved to formazan by mitochondrial dehydrogenases (WST-1 Assay Protocol for Cell Viability, MERCK®). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... and GenElute™ Plasmid Miniprep Kit (Merck), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... we used Sigma-Aldrich kit from Merck-Sigma ...
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bioRxiv - Immunology 2023Quote: ... using Mouse Kapa Genotyping kit (Merck-Millipore), primers (500 nM ...
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bioRxiv - Molecular Biology 2024Quote: Magna MeRIPTM m6A Kit (17-10499, Merck) was used to enable identification and transcriptome-wide profiling of m6A ...
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bioRxiv - Biochemistry 2024Quote: ... cerevisiae using yeast transformation kit (Merck, #YEAST1). The linearized vector was a modified version of pJRoC30 vector (Kindly donated by F.H ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Microbiology 2021Quote: IFN-α and IFN-β or IL-6 and TNF-α were measured in supernatants of AMs or lung lysates using IFN alpha/IFN beta 2-Plex Mouse ProcartaPlex™ immunoassay (ebiosciences) or Milliplex MAP Mouse™ assay (Merck), respectively ...
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bioRxiv - Cancer Biology 2022Quote: ... and VEGF (Cat. No HCYTOMAG-60K) were quantified using Milliplex MAP Luminex microbead assays according to the manufacturer’s instructions (Merck Millipore, Darmstadt, DE). Samples were analyzed without dilution in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... The membranes were later incubated at room temperature for 1 hour with 5% fat free milk in DPBS (D1408, Merck Life Science, Milan, Italy, pH 7.4) and subsequently incubated with the primary antibody overnight at 4 °C (Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 20 million cells using the Magna ChIP A/G Kit (One-day chromatin Immunoprecipitation Kits, Merck) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... These samples were stored in the dark and analyzed colorimetrically using a commercial kit (Spectroquant Sulfide kit, Merck, Schaffhausen, Switzerland). Total particulate nitrogen and carbon were determined by filtration of 100 to 220 mL lake water on pre-combusted (400°C ...
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bioRxiv - Molecular Biology 2020Quote: ... An enhanced chemiluminescence (ECL) detection kit (Merck Millipore) was used to develop the membranes ...
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bioRxiv - Cell Biology 2022Quote: ... the Senescence Cells Histochemical Staining Kit (Merck, UK) was used according to the manufacturer’s guidelines ...
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bioRxiv - Genomics 2022Quote: ... contained in the Kap2G Robust PCR kit (MERCK) per sample and 9 μl added to each well need to be used in a 96-well plate ...
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bioRxiv - Microbiology 2024Quote: ... ECL kits and absolute ethanol were from Merck-Millipore (Germany) ...
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bioRxiv - Cancer Biology 2024Quote: ... as per the manufacturer’s protocol (Merck EZChIP kit). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... Live/dead Double Staining Kit (Merck Life Sciences) was used to stain cells followed by microscopy ...
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bioRxiv - Microbiology 2024Quote: ... After plasmid extraction (Miniprep kit, Sigma-Aldrich/Merck), plasmids were verified by Sanger sequencing at Microsynth.
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bioRxiv - Biochemistry 2024Quote: ... Transfection with NovaCHOice transfection kit (Merck, Nottingham, UK) was performed according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: To evaluate the angiogenic potential of BEC CM a tube formation assay was performed using the HCMEC/D3 line (SCC066 Merck Millipore, Darmstadt, Germany), as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: For the carbon assays the minimal medium was supplemented with the RPMI-1640 amino acid solution (Merck, Cat. No. R7131; 50 mL/L) as nitrogen sources ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of RNA was reverse transcribed using a First Strand cDNA Synthesis Kit (K1622; Fermentas, Burlington, ON, Canada) and polymerase chain reaction (PCR) using a SYBR Green qPCR kit (Merck, Darmstadt, Germany) with incubation at 37°C for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Duolink® PLA Red Starter Kit (Merck, Cat # DUO92101), which allows for endogenous detection of protein interactions was used to detect ACTN4/NMM IIA interaction ...
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bioRxiv - Bioengineering 2020Quote: ... or ApopTag® ISOL fluorescence apoptosis detection kit (Merck Millipore ...
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bioRxiv - Pathology 2020Quote: A Milliplex Magnetic Bead array kit (Merck Millipore, Milan) was used for measuring 30 cytokines ...
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Following exposure with the enhanced chemiluminescence kit (Merck, USA), the bands were processed using Image J software (National Institutes of Health ...
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bioRxiv - Genetics 2024Quote: ... CpGenome Direct Prep Bisulfite Modification kit (Merck Millipore, USA), innuCONVERT (Analytik Jena ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed twice with PBS at room temperature (RT) and lysed in 1x (radio immunoprecipitation assay buffer (RIPA buffer, Merck Millipore, cat. no. 20-188) containing 0.1% SDS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we followed Magna-Chip™ A/G kit (from Merck) protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... ApopTag plus peroxidase in situ apoptosis detection kit (Merck Millipore) was used according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... GLP-1 (GLP-1 total ELISA kit, Merck, Darmstadt, Germany) and biochemical parameters including ALT ...