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Citations for Merck :
1201 - 1250 of 1905 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and the cells grown in 2 L of Overnight Express Instant LB medium (Merck, Germany) containing 100 μg/ml ampicillin at 28 °C for approximately 32 h ...
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bioRxiv - Cancer Biology 2022Quote: ... cell-free samples were fixed for 1 hour at room temperature with 2% glutaraldehyde (Merck) in 0.1M cacodylate buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets used for FACS were dissolved in 500 ul EDTA-DPBS (#E8008, Merck Millipore), cell pellets used for scRNA sequencing were dissolved in 500 μl PBS (pH 7.3-7.4).
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162 ...
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bioRxiv - Microbiology 2020Quote: Cells were lysed with phosphate buffer saline (PBS) containing 0.1% Triton X-100 (Merck Millipore), according to a standard procedure (54) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed three times in PBS and counterstained with Hoechst 33342 (Merck, B2261). F-actin was detected using Alexa Fluor 488-conjugated phalloidin (ActinGreen 488 ReadyProbes ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 1% beta-Mercaptoethanol (#M3148-100ml, Sigma, part of Merck, Darmstadt, Germany)-supplemented RLT lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Merck, KGAa) with added 10% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Cell Biology 2019Quote: ... Chromosomes of the dividing cells were then G-banded with Wright stain (Merck, Darmstad, Germany). The subsequent cytogenetic analysis and karyotypic description followed the recommendations of the International System for Human Cytogenetic Nomenclature (ISCN 2106).
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bioRxiv - Cell Biology 2019Quote: ... Collagen gels containing cells were prepared by mixing type I rat tail collagen solution (Merck) with 2 x concentrated DMEM (Merck) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed twice with PBS and loaded with 200 μM oleic acid (Merck, O3008) and various Doxycycline concentrations (0.5-2 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling ...
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bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... or in PHH and HepG2-hNTCP-derived cell lines using X-tremeGENE HP reagent (Merck) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... HeLa cells were transfected with 1.2 µg DNA (total) per 3 µL GeneJuice (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Cells were left untreated or treated with IFN-α2b (INTRON A, Merck, 100 IU/ml) or IFN-λ3 (R&D Systems ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed in Lysis Buffer (PBS with 0.1% NP-40) with protease inhibitor (Merck), 1 mM Na3VO4 ...
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bioRxiv - Cell Biology 2020Quote: ... LLC-PK1 cells (EC86121112-F0, ECACC) were incubated at 37°C in Medium 199 (Merck) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the cells were covered with Leibovitz’s L-15 medium without L-glutamine (Merck KGaA). To eliminate the confounding effects of neighboring cells on cytoskeleton arrangement and morphology ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated onto #1.5H coverslips coated with 10 μg/ml fibronectin (Merck, FIBRP-RO), 25 μg/ml laminin (Merck ...
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bioRxiv - Microbiology 2021Quote: ... The cells were harvested after adding 480ul cold MeOH/H2O (80/20, v/v) (Merck) to each sample containing Lamivudine (Sigma ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... Cell viability was assessed by metabolic reduction of resazurin sodium salt (Sigma-Aldrich/Merck #R7017). For the assay ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing medium from producing cells was treated with 15 U/ml DNase I (Merck) and 10 mM MgCl2 for 5 h at 37°C prior to ultracentrifugation ...
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bioRxiv - Microbiology 2019Quote: ... bacterial cells were suspended in trifluoroacetic acid (TFA, Uvasol® for spectroscopy, Merck, Darmstadt, Germany) in a sample/TFA ratio of 1:4 (v/v ...
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bioRxiv - Immunology 2021Quote: ... African green monkey kidney (MARC-145) cells were cultured in Dulbecco’s modified MEM (DMEM, Merck) supplemented with 10 % HI FBS and antibiotics at 37 °C in a humidified 5 % CO2 atmosphere.
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bioRxiv - Molecular Biology 2021Quote: Cells were treated with 100 μM DRB (5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside; Merck, D1916) or with 5 μg/ml α-amanitin (Merck ...
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Peripheral blood and synovial mononuclear cells were isolated by density gradient centrifugation (Biocoll, Merck Millipore) or following enzymatic digestion with hyaluronidase (10µL/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were lysed in Triplextractor and after aqueous and organic phase separation using chloroform (MERCK), RNA was precipitated using isopropanol (Fisher Chemical) ...
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bioRxiv - Cell Biology 2022Quote: ... ANA12 (10 μM [cells] or 1 μg [rats] 34,35; SML0209, Sigma-Aldrich/Merck group, USA) was dissolved in DMSO / Tween 80 / sterile 0.9% NaCl solution ...
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bioRxiv - Plant Biology 2022Quote: Root cell measurements were conducted by staining seedlings with Calcofluor White (Merck, 18909-100ML-F). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were rewashed in PBS-T and incubated with filtered (0.22 µm syringe filter, Merck) fluorescently conjugated secondary antibodies and 20 μM Hoechst 33258 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were either preexposed or not to 5 nM ActD (Merck, #50-76-0) for 4 hr ...
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bioRxiv - Neuroscience 2023Quote: ... cells or tissues were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing 2 mM orthovanadate (Merck-Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... 25,000 cells were plated on ELISpot IP Filter Plates (PVDF membrane, 0.45 um, Merck; MSIPN4550) and fixed at 50°C for 1 h before treatment with 1 ug/ml proteinase K (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...
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bioRxiv - Immunology 2022Quote: ... Peritoneal cavity cells were obtained by lavage with 5 mL PBS + 2 mM EDTA (Merck). Following gentle massage ...
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bioRxiv - Cell Biology 2022Quote: ... the cells on the transwell membrane were permeabilized in 0.5% Triton-X (Merck, Darmstadt, Germany) in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with medium supplemented with 20 μg/ml actinomycin D (Act D; Merck) for 1 h to inhibit cellular transcription ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was added to RPE1-hTERT cells together with 8µg/ml polybrene (Merck Life science). Cells were selected using 5 µg/ml puromycin (Santa Cruz ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass (Merck). After treatments cells were washed 3x with PBS for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized with 0.1% (weight/volume) Triton X-100 (Merck, Cat. no. X100) in PBS and the nuclei were stained with the DNA dye Hoechst 33342 (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... after which cells were lysed by resuspending in 50 μL of BugBuster reagent (Merck, 71456) and incubation under constant rotation for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated for 3 hours at 37°C with PMA (50ng/ml; Merck Darmstadt) and Ionomycin (1 mM ...
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bioRxiv - Biophysics 2023Quote: ... SK-N-BE cells expressing LAMP1-eGFP are treated with 10 µg/mL nocodazole (Merck) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Physiology 2023Quote: ... the cells were passaged using 0.02% EDTA Ca2+/Mg2+-free PBS pH 7.2-7.4 (Merck) once 80% confluency was reached ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 30 min at 4°C with 0.4 % paraformaldehyde (PFA, Merck KGaA) and permeabilized by washing twice with permeabilization buffer (PBS containing 2 % FBS and 0.1 % saponin (Sigma)) ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... Transfected cells were collected after three days and suspended in Hank’s Balanced Salt Solution (Merck). The AAV-containing cell lysate was treated with 250 U/ml benzonase at 37 °C for 30 min without adding MgCl2 ...