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Citations for Merck :
951 - 1000 of 1623 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The light signal was developed by soaking membranes in BM Chemiluminescence Western blotting substrate (POD; Merck, MO, USA) for 1 minute in the dark and either exposed to an X-ray film or scanned in an Amersham Imager 600 (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... the liposome suspension was extruded through a polycarbonate membrane filter with a 200-nm pore size (Merck Millipore). Smaller liposomes were prepared by sonicating the liposome suspension ...
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bioRxiv - Neuroscience 2021Quote: ... 15 min) followed by filtering of the supernatant with a 0.45 μm Millex PVDF membrane filter (Merck Millipore). For subsequent FPLC purification (Fast Protein Liquid Chromatography ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then incubated with 0.5 μg/ml of mouse anti-phosphotyrosine monoclonal antibody 4G10 (Merck, Darmstadt, Germany) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... followed by a mid-filter of 5 μm pore size (Mixed cellulose ester membrane filter; Merck Millipore, USA) and finally through a 0.22 μm collection filter (Cellulose mixed ester membrane filter ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Immunology 2021Quote: ... inserts and cut vector were dialysed for 30 minutes using 0.025 µm MF-Millipore membrane filters (Merck VSWP01300) and purity of final products was confirmed by agarose gels and concentration measured using NanoDrop 2000c (Thermo Scientific ND-2000) ...
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bioRxiv - Biophysics 2022Quote: ... The PVDF membrane was blocked in 5 w/v% milk (skim milk powder for microbiology, Millpore, Merck KGaA) in PBS for 1 hour on an orbital shaker (SC5 ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with a 50,000-fold-diluted HRP-labelled anti-mouse or rabbit IgG antibody (Merck) at room temperature for 2 hours or more ...
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bioRxiv - Biophysics 2022Quote: ... The protein solution was filtered through 0.22 μm hydrophilic polyethersulfone (PES) membrane filter (Cat#SLGP033NS, Merck Millipore, USA) and stored as 1.0 mL aliquots at −80 °C until crystallization experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were separated by SDS-PAGE gel electrophoresis and then transferred to the PVDF membrane (Merck, USA).
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 M dithiothreitol for SDS-PAGE separation and electrotransferred to a polyvinylidene difluoride membrane (Immobilon-FL; Merck Millipore) and detected by an antibody raised against synthetic peptides of Synechococcus elongatus PCC sp ...
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bioRxiv - Cell Biology 2024Quote: Protein samples were separated by 10-12% SDS-PAGE and electrotransferred to nitrocellulose membranes (Merck Millipore, Cat # ISEQ00010). Immunoblotting was carried out as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... both the decidual layer and the Reichert’s membrane were removed and embryos were fixed in paraformaldehyde (PFA) (Merck) 2% in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was blocked in 1XPBS + 0,1% Tween (1XPBS-T) with non-fat dry milk (5%, Merck Millipore) for 30 minutes at RT and then incubated for 3 hours at RT with a primary antibody resuspended in 1XPBS-T with 5% milk ...
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bioRxiv - Microbiology 2023Quote: ... water was filtered onto 47 mm diameter 0.2 μm pore-sized polycarbonate white membranes (Millipore, Merck, Darmstadt, DE), which were stored at −20°C for future analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of total proteins (20μg) were separated by SDS-PAGE and transferred to a 0.45-mm Immobilon-P PVDF Membrane (Millipore, Merck) and the membrane was incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... all samples were filtered in a 47 mm polytetrafluoroethylene polymer (PTFE) membrane (0.45 μm pores, Merck Millipore, USA) and rinsed with filtered ultra-pure water at least three times in order to prevent any NaClO and KOH contamination ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting and detection were performed on the PVDF membrane with SNAP i.d.® 2.0 Protein Detection System (Merck) by using anti-myc (A00704 ...
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bioRxiv - Neuroscience 2023Quote: ... Retinae were placed in the center of a Whatman® Nuclepore™ track-etched membrane (Merck, Darmstadt, Germany) and covered with a drop of medium ...
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bioRxiv - Biophysics 2023Quote: ... the vesicle suspension was extruded 31 times through a Whatman Nucleopore™ track-etched polycarbonate membrane (Merck, Germany) with 200 nm pore size using a Mini-Extruder (Avanti Polar Lipids ...
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bioRxiv - Biophysics 2024Quote: ... Aggregates were removed by filtering the concentrated sample with a 0.22 µm PVDF membrane spin-column (Durapore, Merck). The flow-through was injected onto an S75 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... The precipitate was discarded and the supernatant was filtered through a 0.45 µm membrane (Millipore/Merck KGaA, USA).
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bioRxiv - Biochemistry 2024Quote: ... with an appropriate molecular weight cutoff followed by filtration through a 0.45 μm pore size membrane filter (Merck). Protein concentration was determined by absorbance measurements at 280 nm using a NanoDrop OneC spectrophotometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2024Quote: ... the membranes were washed three times for 10 min in PBS-T (PBS with 0.1% Tween-20, Merck). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was next pre-hybridized for 30 min in hybridization buffer (DIG Easy Hyb Granules, Merck, #11796895001) at 65°C ...
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bioRxiv - Biochemistry 2024Quote: Samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated with 3% skim milk dissolved in phosphate-buffered containing 0.05% Tween 20 (PBS-T) ...
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bioRxiv - Cancer Biology 2024Quote: ... Glycoproteins were dot blotted (two dots each) onto a PVDF membrane (Immobilon P, 0.22 µm pore, Merck Millipore), and N- and O-glycans were sequentially released ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were separated on SDS-PAGE gels and transferred to Immobilon-P transfer membranes (cat# IPVH00010, Merck Millipore). The following antibodies were used to probe the membranes ...
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bioRxiv - Cell Biology 2022Quote: ... All assays were performed according to protocols provided by the manufacturer (Luminex/Merck, Poznan, Poland) and were described in detail previously [28–30].
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bioRxiv - Genomics 2021Quote: ... and TNFα) were determined using a custom bovine cytokines MilliPlex xMAP assay (Merck Millipore, USA). Data were recorded on a MAGPIX flow cytometer using Xponent software (Luminex ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1h in base assay medium (D5030, Sigma Aldrich, Merck, Darmstadt, Germany) supplemented with 2 mM glutamine ...
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bioRxiv - Pathology 2020Quote: ... Plasma LH and FSH concentrations were determined using a multiplex assay (MPTMAG-49K, Merck Millipore).
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bioRxiv - Plant Biology 2021Quote: ... the water was removed and replaced with ROS assay solution (100 μM Luminol (123072, Merck), 20 μg mL−1 horseradish peroxidase (P6782 ...
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bioRxiv - Microbiology 2022Quote: ... The assay samples were read on a Luminex 200 instrument with xPONENT software (Merck Millipore), as described by the manufacturer.
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bioRxiv - Immunology 2024Quote: ... Human TGFbeta1 and Human Soluble Cytokine Receptor Magnetic Bead Panel 96-well plate assay (Merck). Data acquisitions were performed on a Luminex® MAGPIX instrument and data were analyzed using the xPONENT® software ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were either left untreated for viability assays or fixed in 70% ethanol (Merck) at −20 °C for 30 min and treated with 25 μg/ml RNaseA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 50 µl LB and deposited on 22 mm filter membranes with 0.45 μm pores (Millipore, Merck) on a pre-dried LB agar plate ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC) membrane (Merck Millipore, ISEQ00010), which was incubated with individual protein-specific primary antibodies at 4 °C overnight on a shaker ...
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bioRxiv - Physiology 2020Quote: ... Proteins in the conditioned medium were concentrated up to 1.2ug/ul of total protein using cellulose membrane Ultra-4 Centrifugal Filter Unit of 3KDa cut-off (Merck) as per the manufacturer instructions.
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bioRxiv - Biochemistry 2021Quote: ... The trace elements stock solution was sterilized by filtration through a 0.22 µm polyether sulfone membrane (Merck-Millipore, Germany). Note that insoluble elements present in this solution were removed by the filtration step ...
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bioRxiv - Microbiology 2021Quote: ... on average 61.3 ± 35.4 liters of groundwater was filtered through 0.2 µm filters (Omnipore Hydrophilic PTFE membrane, Merck Chemicals GmbH) followed by 0.1 µm filters in triplicates (except for well H32 where there were only two replicates out of which one from the November 2018 sampling campaign was used as biological replicate) ...
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bioRxiv - Biochemistry 2020Quote: ... Selected FdR9-containing fractions were concentrated by ultrafiltration using a 30 kDa cut-off membrane (Amicon ultra-15, Merck) and further purified by gel filtration on a Superdex 75 26/60 column (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Biochemistry 2021Quote: ... vesiculosus fucoidan were separated by passing an aqueous fucoidan solution over a 10 kDa cut-off ultrafiltration membrane (Merck) followed by lyophilization.
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bioRxiv - Biochemistry 2020Quote: Proteins separated on PAGE gel were transferred to a PVDF membrane (Immobilon-P/Immobilon-FL, 0.45 μm, Merck Millipore) using Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized total proteins were separated by SDS-PAGE and electro-blotted onto a PVDF membrane (Merck Millipore; Billerica, MA). The membrane was first blocked with 5% skim milk in PBST (Phosphate Buffered Saline with Tween 20) ...
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bioRxiv - Biophysics 2021Quote: ... the culture supernatant was collected and concentrated using the Amicon Ultra centrifugal filter unit with 100 kDa membrane (Merck), aliquoted and stored in -80°C.
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...