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Citations for Miltenyi Biotec :
2351 - 2400 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: The AML blast cells were isolated from whole blood (WB) utilizing MACSprep™ Chimerism CD33 MicroBeads (Miltenyi Biotec), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... leukocytes were purified using magnetic-assisted cell sorting (MACS) using CD45 microbeads (35 μl per sample, Miltenyi Biotec) according to manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec) following manufacturer’s instructions with a 10% reduction in the recommended amounts of reagents ...
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bioRxiv - Neuroscience 2024Quote: ... The prepared cell pellets were resuspended in 80 µl of AstroMACS separation buffer (Miltenyi Biotec, 130-117-336) per 107 total cells ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with EpCAM magnetic beads and AT2 were collected using the MultiMACS Cell24 Separator (Miltenyi Biotec). Automated cell counting was performed using Nexcelom K2 Cellometer with AO/PI reagent ...
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bioRxiv - Microbiology 2022Quote: ... Migratory cells were either used directly for experiments or subjected to a positive selection using CD1a Microbeads (Miltenyi Biotec), in order to enrich epidermal Langerhans cells ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched from spleen with CD4 microbeads (Clone: L3-T4, Miltenyi Biotec, San Diego, CA, USA). The enriched CD4+ T cells were stained with antibodies to define TFH cells as described in flow methods below ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in the sorting buffer and resuspended in the same buffer with anti-FITC Microbeads (Miltenyi Biotec), and incubated on a rotor for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Cancer Biology 2019Quote: ... the resultant single-cell preparation was stained with anti-F4/80 MicroBeads UltraPure mouse Abs (Miltenyi Biotec, Sunnyvale, CA). The sorting column was fixed on a MACS stand (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+) MicroBeads (Miltenyi Biotec 130-091-224) and isolated using MS columns (Miltenyi Biotec 130-042-201 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD133 and HER2 expression on the tumor cell lines were detected using anti-CD133 (130-098-826, Miltenyi Biotec) and anti-HER2 (BMS120Fl ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2020Quote: ... (CD45/CD11b/CD31/Sca1)-positive cells were depleted by passage through LS selection columns (Miltenyi Biotec # 130-042-401). All washes ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... FACS analysis of cell fluorescence was performed on a MACSQuant Analyzer (Miltenyi Biotec, excitation 485 nm, emission 535 nm) by calculating median over 25000 events ...
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bioRxiv - Neuroscience 2020Quote: ... and CD11b+ CD11c+ cells were isolated from adult murine retinas using the Miltenyi Adult Brain Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2020Quote: ... PBMCs or purified NK cells were stimulated for 16-18 hours with 10 ng/mL rhIL-12 (Miltenyi Biotec), 100 ng/mL rhIL-15 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...
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bioRxiv - Cancer Biology 2020Quote: ... CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2021Quote: Primary murine naïve CD4 T cells were isolated from the spleens and lymph nodes of SLE1.2.3 or control mice using the CD4+CD62L+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec). CD62L positive selection was routinely ∼98% in purity as determined by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... PE anti-human IgA-stained bacteria were incubated with Anti-PE Magnetic Activated Cell Sorting (MACS) beads (Miltenyi Biotec) (1:5 ...
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens by using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... CD8+ T cells were isolated by negative selection using magnetic beads following the instructions from the manufacturer (Miltenyi Biotec). The purified CD8+ T cells were incubated with APC-conjugated HLA-A2/YFV NS4b (LLWNGPMAV ...
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bioRxiv - Cell Biology 2022Quote: ... complete DMEM (with FBS) was added to the cell suspension and sorted using MACS LS columns (Miltenyi Biotec GmBH). The columns were washed two times with 1 ml of cold DMEM each ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor and matched normal samples were dissociated into single cell suspension using human tumor dissociation kit (Miltenyi Biotec) and gentleMACS™ Dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell sorting was performed using the FACSAria (Becton Dickinson) or by using magnetic sorting (MACS MicroBead Technology, Miltenyi Biotec) and anti-APC MicroBeads (Miltenyi Biotech) ...
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bioRxiv - Microbiology 2020Quote: ... Mononuclear cells (MNC) were isolated from spleen and lung tissue samples using an OctoMACS tissue dissociation device (Miltenyi Biotec). Lung tissue samples were dissected into approximately 5mm3 pieces and incubated for one hour in a solution of 772.8 U/ml collagenase + 426 U/ml DNase (both from Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... cells were surface-labelled with an APC-conjugated anti-HA antibody (Miltenyi Biotec, Cat# 130-098-404, 1:50) and sorted by flow cytometry ...
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bioRxiv - Developmental Biology 2022Quote: ... Unlabeled cells which passed through the LS column loading CD45 microbeads and LD column (Miltenyi Biotec #130-042-901) retaining weakly labeled cells were defined as the CD45- fraction ...
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bioRxiv - Immunology 2022Quote: Splenic CD4+ T cells were purified from Cd226−/− 2D2 TCR transgenic mice by using mouse CD4 MicroBeads (Miltenyi Biotec). Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11 ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were sorted from pooled spleens of WT C57BL/6 mice by using mouse CD11c MicroBeads (Miltenyi Biotec). Then ...
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bioRxiv - Molecular Biology 2019Quote: Mitochondria were isolated from WT and NSUN2 KO HEK293T cells (1 × 107) using the Mitochondria Isolation Kit (Miltenyi Biotec). Steady-state levels of subunit proteins of mitochondrial respiratory chain complexes were analyzed by immunoblotting with Total OXPHOS Rodent WB Antibody Cocktail (1:250 ...
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bioRxiv - Systems Biology 2019Quote: Cells were washed once with PBS and stained with CD11b-APC or isotype control (Rat IgG2b-APC, Miltenyi Biotec) antibodies in the presence of human FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... and TCRβ+CD138+ and TCRβ+CD138− cells were further separated with anti-PE magnetic MicroBeads (Miltenyi Biotec, Auburn, CA). After three washes with PBS ...
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bioRxiv - Immunology 2020Quote: ... ΔLNGFR-positive transduced T cells were isolated using the human CD271 MicroBead kit (Cat# 130-099-023, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then collected and processed for intracellular staining with anti-IFN-γ-FITC monoclonal antibody (Miltenyi Biotec, Germany). Changes in the subset of lymphocyte cells and cytokine production (IFN-γ ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) from healthy donors (HD) were used to isolate monocytes (using CD14+ microbeads, Miltenyi Biotec) and to sort CD1c+ DC and slanDCs using FACS (Aria IIIu ...
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bioRxiv - Cancer Biology 2020Quote: ... Positive selection was performed to isolate CD8+ T cells from primary tumors and spleens using CD8a microbeads (Miltenyi Biotec), an LS column ...
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bioRxiv - Cell Biology 2021Quote: T lymphocytes were isolated from healthy donor’s PBMCs via Pan T Cell Isolation Kit (Miltenyi Biotec,130-096-535) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Electroporated cells were seeded on Laminin 521 (Biolamina, Sundbyberg, Sweden) coated plates in StemMACS iPS-Brew XF (Miltenyi Biotec) supplemented with 1x CloneR (Stemcell Technologies ...
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bioRxiv - Immunology 2020Quote: ... the cells were labeled with the IFN-γ Catch Reagent (IFN-γ Secretion Assay-Detection Kit, human Miltenyi Biotec) containing bispecific antibodies for CD45 and IFN-γ which was secreted by the stimulated target cells ...
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bioRxiv - Cell Biology 2021Quote: ... CD11b myeloid cells were purified from PBMCs by positive selection using the anti-CD11b microbeads and columns (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQUANT Analyzer (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... human peripheral blood mononuclear cells were activated with 50□ng/mL OKT3 (Cat. No. 130-093-387, Miltenyi Biotec) and harvested for retroviral transduction on day 2 ...
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bioRxiv - Immunology 2020Quote: C1 and C4-plasma generated cells were isolated after 7 d of culture using human CD14 Microbeads (Miltenyi Biotec). Isolated CD14+ cells were validated using flow cytometry (CD14-FITC ...
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bioRxiv - Immunology 2021Quote: ... The Cytokine bead array and the T cell activation and proportions were analyzed using the MACSQuant Analyzer (Miltenyi Biotec).