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Citations for Miltenyi Biotec :
2151 - 2200 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Microglia cells from adult young or old mice were isolated and cultured using immunomagnetic separation (Miltenyi Biotec, Germany). Mice were perfused via the left ventricle with 60 mL of cold saline and collected in HBSS buffer without calcium/magnesium (#14175-05 ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... density gradient centrifugation followed by Magnetic-activated cell sorting using biotin MicroBead kit (Miltenyi Biotec, Leiden, The Netherlands), according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... both naïve and memory OT-1 CD8 T cell donors were magnetically sorted via magnetic beads (Miltenyi Biotec) with specific antibodies ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by negative selection from spleens and lymph nodes (Miltenyi Biotec, #130-104-454) according to manufacturer’s instructions (typically >95% purity) ...
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bioRxiv - Cell Biology 2022Quote: ... ErbB2 (CD34) PE-Vio770 used for FACS sorting of HER2+ stable cells was obtained from Miltenyi Biotec (Germany).
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bioRxiv - Immunology 2022Quote: ... Acquisition of a minimum of 100,000 cells per sample was performed on a MACSQuant 10 analyzer (Miltenyi Biotec). Single-stained controls using mouse Ig/ κ compensation beads (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... fresh splenocytes were isolated as previously described and subjected to Pan-T cell magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: ... Patient-derived GBM6 and GBM12 cells were stained with human-CD133 (PE conjugated 1:11 dilution; Miltenyi Biotec), then with human-CD68 (FITC conjugated 1:20 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... patient cells were stained with the following primary antibodies: mouse anti–human CD3 (clone BW264/56; Miltenyi Biotec), mouse anti–human CD7 (clone M-T701 ...
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bioRxiv - Immunology 2020Quote: ... The excess of beads was washed out and cells were then separated over an LS-column (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... and T cells were depleted using Thy1.1 or Thy1.2 positive selection and a magnetic separation kit (Miltenyi Biotec). Cells were counted ...
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bioRxiv - Immunology 2019Quote: ... Resuspended cells from the bone marrow were labeled using biotinylated anti-CD3 antibody and streptavidin microbeads (Miltenyi Biotec). Cells were then resuspended in PBS and 1×106 cells were transferred to each mouse.
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bioRxiv - Immunology 2019Quote: ... The cells were then labelled with magnetic streptavidin microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Auburn, CA). After washing ...
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bioRxiv - Immunology 2021Quote: ... Released cells were collected in the supernatant and filtered through a 70 mm MACS Smart Strainer (Miltenyi Biotec) into a new 15 mL conical tube ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended pellets to single cells and incubated with Anti-SSEA-1 (CD15) Microbeads (Miltenyi Biotec, 130-094-530). After collected the positive cells ...
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bioRxiv - Cancer Biology 2020Quote: Cells were stained with anti-human CD34 (AC136, APC, Miltenyi Biotec, Cat No 130-113-738, 1:50) and anti-human CD38 (IB6 ...
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bioRxiv - Immunology 2020Quote: ... Tissue was dissociated into single-cell suspensions with enzymic digestions using a gentleMACS dissociate per protocol (Miltenyi Biotec). Single-cell suspensions were cryopreserved (10% DMSO ...
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bioRxiv - Immunology 2021Quote: ... Th1 cultures were activated with CD2/3/28 activation beads (Human T cell Activation/Expansion kit, Miltenyi Biotec) at a 2:1 ratio of cells to beads with anti-IL-4 (2μg/mL) ...
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bioRxiv - Microbiology 2021Quote: Purified rhesus and human CD4+ T cells were isolated from PBMCs using magnetic MACS CD4 MicroBeads (Miltenyi Biotec), as previously described (35) ...
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bioRxiv - Immunology 2020Quote: ... CD45+ hematopoietic cells were depleted using anti-CD45 magnetic beads by autoMACS with the depleteS program (Miltenyi Biotec). Total mTECs (EpCAM+UEA-1+Ly51lo) ...
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bioRxiv - Immunology 2021Quote: ... TCRβ+CD138- and TCR+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). Depending on the experimental objective ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils were isolated from BM cells by positive selection using the neutrophil isolation kit (Miltenyi Biotec, Auburn, CA). WT and G184S KI neutrophils were stained by Alexa Fluor 568- and Alexa Fluor 488-conjugated anti-mouse Gr-1 antibodies ...
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bioRxiv - Immunology 2021Quote: ... Isolated cells were negatively selected tandemly first with Myelin Removal Beads II (Miltenyi Biotec, Cat# 130-096-731) and second with anti-CD11b MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Naïve CD4 T cells were isolated from mice spleens and lymph nodes by magnetic bead separation (Miltenyi Biotec) following the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was achieved using the 37_C Multi_tissue_dissociation B program on the gentleMACS octo-dissociator (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... 0.5% BSA) and then incubated with 10µL per 106 cells each of IFN-γ Catch Reagent (Miltenyi Biotec), IL-2 Catch Reagent (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
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bioRxiv - Biochemistry 2022Quote: ... Separation of yeast populations was done via magnetic-activated cell sorting (Miltenyi Biotec, cat. no. 130-091-051) using either streptavidin (Miltenyi Biotec ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... primary ACC from the mouse was dissociated as single cells using the mouse Tumor Dissociation Kit (Miltenyi Biotec). Cells were then serially transplanted into NSG mice for approximately 6 months ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany] ...
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bioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were isolated using the CD34 MicroBead Kit or CliniMACS (catalogue no. 130-100-454; Miltenyi Biotec). Patients gave informed consent in accordance with the Declaration of Helsinki and with approval of the National Health Service Greater Glasgow Institutional Review Board and Clyde Institutional Review Board ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared cell pellets were resuspended in 80 µl of AstroMACS separation buffer (Miltenyi Biotec, 130-117-336) per 107 total cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended and subjected to Miltenyi debris removal reagent (cat # 130-109-398, Miltenyi Biotec Inc) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The dissociated single-cell suspension was resuspended and incubated in FcR Blocking Reagent (130–097-678; Miltenyi Biotec) at 4 °C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1 x 106 RFP+ cells were collected in 0.04% BSA in PBS (Miltenyi Biotec 130-091-376) for single-nuclei isolation and multi-ome ATAC/sequencing (10X Genomics) ...
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bioRxiv - Developmental Biology 2023Quote: ... These samples were further processed using Debris Removal Solution and Dead Cell Removal Kit (Miltenyi Biotec., Gaithesburg, MD). Red blood cell depletion from cell suspensions were performed using anti-Mouse Ter-119 antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: Splenic naïve B-cells were prepared using the CD43 negative selection commercial kit (Miltenyi Biotec.; 130-090-862). Spleens were pulled from 2-3 sacrificed sex- and age- matched animals mechanically disintegrated and filtered through a 70 µm cell strainer to prepare a single cell suspension essential for microbead separation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 90µL of a buffer comprised of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB ...
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bioRxiv - Immunology 2023Quote: ... Cells harboring the receptor were enriched by using biotinylated anti-EGFR antibody and anti-biotin microbeads (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Monocyte-derived dendritic cells and monocytes isolated with the CD14 MACS kit from Miltenyi biotec (130-050-201) were collected in a V-bottom plate at 105 cells/well (Thermo scientific # 10462012 ...
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bioRxiv - Immunology 2023Quote: ... Bead-bound cells were positively selected through a magnet-mounted MACS LS column (Miltenyi Biotec; 130-042-401). In some experiments ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry analysis were conducted using BD LSR Fortessa™ cell analyzer or MACSQuant® analyzer (Miltenyi Biotec). Data were prepared using FlowJo 10.1 (Tree Star ...
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bioRxiv - Cancer Biology 2023Quote: ... GCB cells were first enriched from mouse splenocytes using the PNA MicroBead Kit (Miltenyi Biotec, 130-110-479), followed by antibody incubation and sorting on BD Aria II sorter (RRID:SCR_018091 ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently enriched for CD34+ cells using the human CD34 MicroBead kit (130-046-702 MACS Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Physiology 2023Quote: Single-cell suspensions were prepared from mouse lungs using the Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions ...