Labshake search
Citations for Miltenyi Biotec :
2601 - 2650 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Each tumor was dissociated into a single cell suspension with Miltenyi’s gentleMACS Dissociator according to the tumor dissociation kit protocol (Miltenyi Biotec, Germany). A small portion of the dissociated tumor cells was removed for profiling via flow cytometry ...
-
bioRxiv - Developmental Biology 2019Quote: ... cortices from 1-3 embryos from 2-5 mothers were pooled and dissociated into single cell suspension using the MACS Neural Tissue Dissociation kit with papain (Miltenyi Biotec) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... Neutrophils were counted using an Invitrogen automated cell counter and the purity assessed by flow cytometry using FITC anti-CD16 and APC anti-CD15 antibodies (Miltenyi Biotec) as markers characteristic of human neutrophils.
-
bioRxiv - Immunology 2019Quote: CD4+ and CD14+ cells were enriched from the PBMCs of 3 healthy donors via magnetic separation using the corresponding MicroBeads (Miltenyi Biotec). The CD14+ fraction was cryopreserved in liquid nitrogen ...
-
bioRxiv - Immunology 2019Quote: Human monocytes were purified from peripheral blood mononuclear cells (PBMCs) from healthy donors by positive immunoselection with anti-CD14-conjugated MACS beads (Miltenyi Biotec). Monocytes were also purified from PBMCs from either HLA-A2 WT NOD2 donors or HLA-A2 homozygous mutant NOD2 Crohn’s patients ...
-
bioRxiv - Bioengineering 2019Quote: ... the cells were washed two times with PBS and the percentage of PI stained cells was determined using a MACSQuant VYB system (Miltenyi Biotec). Data analysis was performed using the FlowJo software.
-
bioRxiv - Bioengineering 2019Quote: ... The monocytic cell fraction was isolated from the hPBMC fraction via negative selection using the MACS pan-monocyte isolation kit (Miltenyi Biotec) according to the supplier’s instructions ...
-
bioRxiv - Immunology 2019Quote: B220+ B cells were recovered from bone marrow mononuclear cells from WT or CCR7−/− mice by negative selection using a MACS microbeads isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2019Quote: ... Fresh BM aspirates from MM patients were routinely depleted from malignant plasma cells using anti-CD138-coated magnetic beads (Cat. #130051301 Miltenyi Biotec) and used for RNA sequencing and functional assays.
-
bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2020Quote: ... the population of IFNγ secreting cells was identified using the IFNγ Secretion assay detection kit (PE) (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
-
bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
-
bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
-
bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
-
bioRxiv - Physiology 2021Quote: Bone marrow monocytes and neutrophils were isolated from C57BL/6J mice using MACS cell separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation with either anti-Ly6G-PE (clone 1A8 ...
-
bioRxiv - Neuroscience 2019Quote: ... or left untransduced for 4 days prior to lysis in mitochondria isolation kit lysis buffer (1×107 cells/mL; Miltenyi Biotec) with protease inhibitors (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... Pulse-treated cells and non-treated cells harboring a different fluorophore were then mixed in a 50:50 ratio confirmed by flow cytometry (MACSQuant; Miltenyi Biotec). Gates were established using non-fluorescent parental cell lines as a negative control ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then depleted of lineage-positive cells by passing through a magnetic separation column (MACS LD Column, Miltenyi Biotec) loaded on a DynaMag-5 Magnet (Invitrogen) ...
-
bioRxiv - Cell Biology 2021Quote: ... The dispersed islet cells were then resuspended in 150 ml of Nicoletti buffer containing 50 mg/ml propidium iodide (Miltenyi Biotec), 0.1% (wt/v ...
-
bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
-
bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... 400 μL of 1x AnnexinV Binding buffer was added to each tube and cells were analyzed on a MACSQuant VYB (Miltenyi Biotec). A minimum of 10 000 cells were analyzed per condition ...
-
bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
-
bioRxiv - Physiology 2022Quote: ... The cell pellet was re-suspended in a sorting buffer and then passed through a magnetized LS separation column (Miltenyi Biotec). The iron-containing superparamagnetic cells/aggregates were eluted from the demagnetized column ...
-
bioRxiv - Immunology 2022Quote: ... Scotland) followed by CD14+ selection via magnetic cell separation using MACS CD14 MicroBeads and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), as previously described (31) ...
-
bioRxiv - Immunology 2022Quote: ... we assessed the capacity of NK cells to perform ADCC after being stimulated with IFN-α or IL-15 (Miltenyi Biotec). In such cases ...
-
bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells were resuspended with 100 µl of Inside Perm Buffer (Inside Stain Kit, Miltenyi Biotec). Eight µl of anti-GFAP and 2.5 µl of anti-Iba1 were added to the cell suspensions and mixed by gently pipetting ...
-
bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells resuspended with 400 µl of Running Buffer composed of MACS BSA stock solution (Miltenyi Biotec) 1:20 with autoMACS Rinsing Solution (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2022Quote: ... Biopsies were manually homogenized using sharp dissection between digest steps and by blunt homogenization through a 100 μm cell strainer (Miltenyi Biotec). Centrifugation steps were performed at 300 x g for 5 min at 4°C.
-
bioRxiv - Immunology 2020Quote: ... the SILP and the CLP single cell suspensions were obtained using the Lamina Propria Dissociation Kit (Miltenyi Biotec SAS, Paris, France) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... depleted of CD8+ T cells using a negative selection step (CD8 MicroBead Kit; LD columns, QuadMACS Separator, all from Miltenyi Biotec) per manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: ... The isolation of lineage negative (Lin-) HSPCs or neutrophils were performed using Lineage Cell depletion kit or Neutrophil Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
-
bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
-
bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
-
bioRxiv - Neuroscience 2022Quote: ... The samples were passed through a 45 μm Nitex mesh to remove undissociated cell clumps and after addition of myelin removal beads (Miltenyi Biotec), passed through a MACS column (Miltenyi Biotec) ...
-
bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
-
bioRxiv - Immunology 2022Quote: ... Fluorescence spillover compensation for cell sorting was performed using lymphocytes stained with CD4 (OKT4) in the respective colors and with compensation beads (Miltenyi Biotec) for flow cytometry ...
-
bioRxiv - Cell Biology 2022Quote: ... incubated for 15 min at room temperature and applied onto the dead cell removal column (cat # 130-042-401, Miltenyi Biotec), which was attached to the MACS separator ...
-
bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... Remaining cells were then magnetically labeled with HLA-G – PE antibody and anti-PE microbeads and enriched for HLA-G+ EVTs by magnetic-activated cell sorting using an OctoMACS separator and MS columns (Miltenyi Biotec).
-
bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens of transplanted PyMT tumor-bearing mice with a CD8a+ T Cell Isolation Kit (#130-104-075, Miltenyi Biotec), according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2019Quote: ... The cell suspension was passed through an LS column to hold the micro bead-labelled cells (RGCs) in the magnetic field of a MidiMACS system (Miltenyi Biotec). The adherent RGCs were rinsed with 3 ml of DPBS/BSA buffer to deplete endothelial cells and microglia ...
-
bioRxiv - Cancer Biology 2019Quote: ... The cells were then washed twice prior to resuspension in FACS buffer and acquisition on the MACSQuant Analyzer 10 (Miltenyi Biotec). Data were analyzed with FlowJo software (v10 ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were enriched from the filtrates by Ficoll density gradient centrifugation and magnetic-activated cell sorting using CD34 antibody-conjugated microbeads (Miltenyi Biotec). CD34+ cells were grown in serum-free StemSpan SFEMII (Stemcell Technologies ...
-
bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cells using dissociation kits according to the manufacturer’s protocol (preserving all surface epitopes, Miltenyi Biotec #130-095-927), then stained with APC-conjugated antibodies against CD45 (eBioscience #17-0451-83) ...