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Citations for Miltenyi Biotec :
2201 - 2250 of 2875 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... germ cells were recovered using an anti-SSEA-1 antibody conjugated to MicroBeads (Miltenyi Biotec, 130-094-530) in accordance with the manufacturer’s instructions.
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... FLK1 expressing cells were sorted for by separation using a MACS LS column (Miltenyi Biotec, 130-042-401). FLK1 expressing HB cells were then taken for flow cytometry analysis and sequencing experiments as well as further differentiation in blast culture into HE1 ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were removed from the samples using RBC lysis reagent (Miltenyi Biotec, cat.no.130-094-183). After RBC lysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated and expanded with the T Cell Activation/Expansion Kit (Miltenyi Biotec, Cat No. 130-091-441) according to the vendor’s manual ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... CD11b-positive brain cells were selected from the brain with microbeads-conjugated CD11b antibody (clone M1/70.15.11.5; Miltenyi Biotec) and CD4-positive spleen cells from the spleen with Dynabeads magnetic beads-conjugated CD4 antibody (clone L3T4 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were suspended in 90µL of a buffer made of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB) ...
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bioRxiv - Neuroscience 2023Quote: ... OPC magnetic cell sorting was performed using anti-O4-coupled-beads (O4 MicroBead Kit, Miltenyi biotec, Paris, France) and MultiMACS Cell24 Separator Plus (Miltenyi biotec ...
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bioRxiv - Immunology 2023Quote: ... the enriched CD8+ T cells were stimulated with the CMVpp65 overlapping peptide pool PepTivator® CMVpp65 (Miltenyi Biotec) at a concentration of 1 µg of each peptide/ml for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... pDCs were enriched from freshly isolated PBMCs using the human Plasmacytoid Dendritic Cell Isolation Kit II (Miltenyi Biotec) following manufacturer’s instructions with a 10% reduction in the recommended amounts of reagents ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Cat #130-104-075). Rag1-/- mice were reconstituted with CD8+ T cells by intravenous route (3 x 106 cells/mouse ...
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bioRxiv - Immunology 2023Quote: ... The splenocyte and tumor cell suspensions were then fractionated using CD8 (TIL) microbeads (Miltenyi Biotec, 130-116-478) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and B cells were isolated by negative selection directly from whole blood using MACSxpress® separator (Miltenyi Biotec) with Whole Blood B Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were incubated with APC-conjugated cytochrome c antibody (1: 50 dilution in permeabilisation buffer)(Miltenyi Biotec) and analyesd by FACS on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended with permeabilization buffer and incubated with 20 μl FcR blocking reagent (Miltenyi Biotec) for 10 min at 4°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: For CD44 staining gliomaspheres were dissociated into single cells and stained with anti-CD44-APC antibody (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PBMCs were depleted of CD14 mononuclear cells using anti-CD14 microbead antibodies (cat#130-050-201, Miltenyi Biotec) and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101 ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells (PBMC) were purified from whole blood by density gradient centrifugation (FicollPaqueTM, Miltenyi Biotec). Whole blood was collected from healthy donors through an NIH Department of Transfusion Medicine (DTM)-approved protocol (Institutional Review Board of the National Institute of Allergy and Infectious Diseases [NIAID]) ...
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bioRxiv - Bioengineering 2023Quote: ... This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec) for the subsequent experiments.
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bioRxiv - Neuroscience 2023Quote: OPCs were isolated from p7 mouse brains using magnetic activated cell sorting and anti-NG2 MicroBeads (Miltenyi Biotec). Tissue dissection and cell sorting were performed under sterile conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and labeled with CD154-Biotin followed by anti-Biotin MicroBeads (CD154 MicroBead Kit; Miltenyi Biotec) and magnetically enriched by two sequential MS columns (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed twice with 1X assay buffer before being assessed by a MACSquant analyzer (Miltenyi Biotec, Germany) with a green fluorescence channel using a 488 nm laser source ...
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bioRxiv - Microbiology 2024Quote: ... Purity of CD4+ T cell population was measured by flow cytometry (MACSquant Analyzer using MACSQuantify software, Miltenyi Biotec), showing a fraction of at least 95% CD4+CD3+ double positive cells and less than 0.01% CD123+CD304+CD303+ cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells in DMEM+FBS were subsequently filtered through a 70µM filter (Miltenyi Biotec, Cat-Nr: 130-098-458) and transferred to 15ml conical Falcon tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were isolated for imaging using magnetic purification (102) with a magnetic cell fraction column (MACS, Miltenyi Biotec) and a MidiMACS separator ...
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bioRxiv - Immunology 2024Quote: ... The cells were isolated from the colon lamina propria using the “Lamina Propria Dissociation Kit” from Miltenyi Biotec according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... ∼30h after transfection cells were submitted to MACS column selection using MS columns (130-042-201, Miltenyi Biotec) and LNGFR MicroBeads (130-091-330 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured in medium supplemented with 5 μM of the ROCK inhibitor StemMACS™ Y27632 (Miltenyi Biotec). The differentiation of hiPSCs into midbrain neurons was performed as previously described (35) ...
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bioRxiv - Microbiology 2024Quote: Primary CD4+ T cells were purified from PBMCs of a healthy donor by negative selection (Miltenyi Biotec, USA). Purified CD4+ T cells were stimulated with 1 µg/mL soluble anti-CD3 (clone OKT3 ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumors were dissociated into single-cell suspension using the tumor dissociation kit (Miltenyi Biotec, 130-095-929) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec) and published protocols 50,51 ...
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bioRxiv - Immunology 2021Quote: ... Bone marrows cells were purified with CD3+ T isolated kit (CD3ε MicroBead Kit, mouse, 130-094-973, Miltenyi Biotec). Splenic CD4+ and CD8+ cells were purified in two steps ...
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bioRxiv - Immunology 2020Quote: ... OVA-specific CD4+EGFP− cells T conventional cells were FACS-sorted from the spleen of OT-II.Ly5.1.Foxp3EGFP mice after enrichment using CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Cell Biology 2022Quote: ... The cells were incubated with EpCAM magnetic beads and AT2 were collected using the MultiMACS Cell24 Separator (Miltenyi Biotec). Automated cell counting was performed using Nexcelom K2 Cellometer with AO/PI reagent ...
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bioRxiv - Microbiology 2022Quote: ... Migratory cells were either used directly for experiments or subjected to a positive selection using CD1a Microbeads (Miltenyi Biotec), in order to enrich epidermal Langerhans cells ...
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bioRxiv - Immunology 2019Quote: ... CD4+ T cells were enriched from spleen with CD4 microbeads (Clone: L3-T4, Miltenyi Biotec, San Diego, CA, USA). The enriched CD4+ T cells were stained with antibodies to define TFH cells as described in flow methods below ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed in the sorting buffer and resuspended in the same buffer with anti-FITC Microbeads (Miltenyi Biotec), and incubated on a rotor for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Cancer Biology 2019Quote: ... the resultant single-cell preparation was stained with anti-F4/80 MicroBeads UltraPure mouse Abs (Miltenyi Biotec, Sunnyvale, CA). The sorting column was fixed on a MACS stand (Miltenyi Biotec ...
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bioRxiv - Developmental Biology 2021Quote: ... Differentiating c-KIT+ neonate spermatogonia cells were magnetically labeled with CD117 (c-KIT+) MicroBeads (Miltenyi Biotec 130-091-224) and isolated using MS columns (Miltenyi Biotec 130-042-201 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD133 and HER2 expression on the tumor cell lines were detected using anti-CD133 (130-098-826, Miltenyi Biotec) and anti-HER2 (BMS120Fl ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary human T cells were isolated from PBMCs by negative selection using the Pan T Isolation Kit (Miltenyi Biotec). Batch 1 T cells were cultured in IMDM Medium containing Icove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2020Quote: ... (CD45/CD11b/CD31/Sca1)-positive cells were depleted by passage through LS selection columns (Miltenyi Biotec # 130-042-401). All washes ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were then co-incubated with 1 μg of APC-conjugated anti-IgG antibody (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 μg of PE-conjugated anti-Flag antibody (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... FACS analysis of cell fluorescence was performed on a MACSQuant Analyzer (Miltenyi Biotec, excitation 485 nm, emission 535 nm) by calculating median over 25000 events ...
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bioRxiv - Neuroscience 2020Quote: ... and CD11b+ CD11c+ cells were isolated from adult murine retinas using the Miltenyi Adult Brain Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2020Quote: ... PBMCs or purified NK cells were stimulated for 16-18 hours with 10 ng/mL rhIL-12 (Miltenyi Biotec), 100 ng/mL rhIL-15 (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... NK cells highly enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion kit (Miltenyi Biotec), were used as effector cells ...