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Citations for Miltenyi Biotec :
2551 - 2600 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... IMhu-M cells were cultivated in IL-4/GM-CSF MoDC differentiation medium (Miltenyi Biotec, Cat. No. 130-094-812), when indicated ...
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bioRxiv - Genomics 2024Quote: ... we processed a fraction of the dissociated tumor for immune cell depletion using CD11b MicroBeads (Miltenyi Biotec #130-097-142) following manufacturer’s protocol on LS Columns ...
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bioRxiv - Immunology 2023Quote: ... and the supernatant was discarded while PMs were purified according to manufacturer’s instruction using a magnet purification kit by selecting F4/80+ cells (Miltenyi Biotec; Macrophage Isolation Kit ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Genomics 2024Quote: The obtained homogenate was filtered in 3-5 ml of Wash Buffer (1x DPBS, containing 10% FBS, 20mM HEPES, and 6mM glucose) through a prewetted 70μm cell culture filter (Miltenyi Biotec) and centrifuged for 5 minutes at 300xg (4 °C).
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bioRxiv - Microbiology 2024Quote: ... Primary CD4+ T-cells and monocytes were purified from PBMCs by positive selection using CD4 (Miltenyi Biotec; 130-045-101) and CD14 (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: IFN-γ production of CD8+ T cells was measured by adapting a commercially available IFN-γsecretion assay kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were blocked for 30 min and stained with 2μl of human EpCAM (FITC conjugated, Miltenyi Biotec, 130-080-301) and 1:75 of mouse MHC class I (APC conjugated ...
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bioRxiv - Biochemistry 2024Quote: ... cells were incubated for 20 min at 4°C with an anti-mouse antibody conjugated to magnetic microbeads (Miltenyi Biotec). Finally ...
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bioRxiv - Microbiology 2023Quote: ... cells were permeabilized and stained in buffer from commercial kit (Inside Stain Kit, ref no. 130-090-477, Miltenyi Biotec) for 40 minutes at 4°C ...
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bioRxiv - Genomics 2023Quote: Human induced pluripotent stem cell lines were routinely cultured on Matrigel-coated dishes in MACS iPS-Brew media (Miltenyi Biotec) and passaged using ReLESR (Stem Cell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: The fluorescence intensities of the recovered cells were analyzed using a MacsQuant Analyzer flow cytometer (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and epithelial cells were selected using CD326 (EpCAM) magnetic beads (respectively 130-052-301 and 130-105-958, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ expressing thymocytes were depleted using anti-CD4 and CD8 magnetic-activated cell-sorting (MACS) beads (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Immunology 2023Quote: ... bacterial cells were washed again and re-suspended in PBS before they were analyzed with a MACSQuant cytometer (Miltenyi Biotec). C3 deposition analysis in age-matched WT ...
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bioRxiv - Genomics 2023Quote: All other solid organs were dissociated into single-cell suspensions using Miltenyi’s Liver Dissociation Kit (Miltenyi Biotec, 130-105-807) with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were isolated from mouse spleen and lymph nodes by magnetic bead sorting using the Naïve CD4+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... tumour suspensions were mashed through a 70 μm filter and live cells were enriched using debris removal solution (Miltenyi Biotec). For obtaining single cell suspensions for the KM macrophage datasets ...
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bioRxiv - Cell Biology 2023Quote: CD34+ magnetic cell isolation was performed on the PBMCs according to the manufacturer’s protocol for the Direct CD34+ progenitor cell isolation kit (Miltenyi Biotec), to enrich for haematopoietic progenitor cells ...
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bioRxiv - Neuroscience 2023Quote: ... Magnetic beads coupled with mouse anti-CD11B antibodies (microglia/macrophage) were used for cell isolation according to the manufacturer’s protocol (Miltenyi Biotec) as previously described [29 ...
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bioRxiv - Microbiology 2023Quote: Cecal and colonic lamina propria cells were extracted using the Lamina Propria Dissociation Kit and gentleMACS Dissociators (Miltenyi Biotec, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... macrodissected HCC nodules and control liver samples were dissociated as single-cell suspensions using the Liver Dissociation kit (Miltenyi Biotec) and the gentleMACS Octo Dissociator following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... MNCs were enriched in CD34+ cells using magnetic separation with the CD34 Microbead Kit (Miltenyi Biotec, San Diego, CA, USA) and further purified by flow cytometric sorting as described below.
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bioRxiv - Microbiology 2024Quote: ... These cells were then subjected to staining with PE Anti-human IgA antibodies (1:100; Miltenyi Biotec clone IS11-8E10) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed twice with FACS buffer and analyzed using flow cytometry (MacsQuant Analyzer 10 flow cytometer, Miltenyi Biotec). The FlowJo™ software (BD Biosciences ...
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bioRxiv - Neuroscience 2024Quote: ... The cell dissociation procedure followed the guidelines of the Neural Dissociation Kit (T) (130-093-231, Miltenyi Biotec GmbH, Germany). After filtering with medium with 10 % (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were then stained with fluorochrome-conjugated antibodies (anti-mouse-BV421-CD45) and anti-mouse-PE-O4 (Miltenyi Biotec)) for 40 min at 4°C to characterize oligodendrocytes (CD45-O4+) ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: ... lung single-cell suspensions were labeled with anti-CD4+ or anti-CD8+ magnetic beads and separated by autoMACS (Miltenyi Biotec), or CD4+CD8+ double positive cells purified from thymus single-cell suspensions by flow-cytometric sorting on FACS Aria (BD Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Cells were then incubated with conjugated antibodies (FITC-CD11b, BD Biosciences, #557396; PE-Recombinant-ACSA2, Miltenyi Biotec, #130-116-244) for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... hSS2 mouse tumors were harvested and digested to single cell suspension with the Miltenyi mouse tumor dissociation kit (Miltenyi Biotec). Approximately 5-10 million cells were crosslinked with 1% of formaldehyde ...
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bioRxiv - Biochemistry 2024Quote: We purified CD4+CD25-CD44lowCD62Lhigh naïve T cells obtained from the spleens of WT and Cars2+/– littermates with the Naïve CD4+ T Cell Isolation Kit II (130-093-227; Miltenyi Biotec) and an autoMACS Pro Cell Separator (Miltenyi Biotech) ...
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bioRxiv - Cancer Biology 2024Quote: Miltenyi bead separation was used according to the manufacturer’s instructions to purify CD8 T cells from the spleen tissue of WT FVB mice (#130-104-075, Miltenyi Biotec). Four subsets of tumor-infiltrated myeloid cells (CD45+CD11b+Ly6G- ...
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bioRxiv - Immunology 2024Quote: Macrophages were isolated from lung single-cell suspensions using microbeads conjugated to anti-F4/80 antibodies according to the manufacturer’s protocol (Miltenyi Biotec). CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... which were negatively selected using a magnetic cell sorting system (MACS) consisting of CD11b and CD45 MicroBeads and MS Columns (Miltenyi Biotec), were used as BM-MSCs ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM EDTA in PBS) and endothelial cells (CD31+) and leukocytes (CD45+) were sequentially positively selected using CD31 and CD45 MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... Monocytes were isolated from PBMCs using a magnetic cell separation system with anti-CD14 mAb-coated microbeads (130-050-201; Miltenyi Biotec). CD14-positive monocytes were differentiated in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... Stem cell marker expression was validated in isolated cell lines by flow cytometry using anti-SSEA4-FITC (Cat. # 130-098-371, Miltenyi Biotec).
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bioRxiv - Neuroscience 2020Quote: ... Resuspended total EVs were incubated with 4 μg of anti-GLAST (i.e. antibody against the astrocyte cell surface antigen-1; RRID: AB_2733473; Miltenyi Biotec, Auburn, CA), anti-human CD171 (i.e ...
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bioRxiv - Neuroscience 2021Quote: ... with either magnetic-activated cell sorting (MACS) using large cell columns and CD90 pre-conjugated to microbeads (#130-042-202 and #130-049-101, Miltenyi Biotec), fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2021Quote: Spleens of OT-I Hobit reporter × ROSA26-eYFP LT mice were isolated and subsequently CD8+ T cells were isolated by negative selection using MicroBeads (130-104-075, Miltenyi Biotec) according to the manufacturer’s protocol ...