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Citations for Miltenyi Biotec :
2401 - 2450 of 3039 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cDC2s were further enriched from single cell suspensions by positive isolation with CD11c Microbeads (Miltenyi Biotec; per manufacturer’s instructions) and FACS sorting (MHCII+ CD11c+ CD64- F4/80- CD172a+ XCR1- ...
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bioRxiv - Immunology 2022Quote: Mouse tissue samples for scRNA-seq were first positively selected for CD45+ cells using Mouse CD45 Microbeads (Miltenyi Biotec) and then sorted for 7AAD-CD45+Zbtb46-GFP+ expression before submission ...
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bioRxiv - Genomics 2022Quote: ... and processed into single-cell suspensions using the Whole Skin Dissociation Kit (Miltenyi Biotec, Cat No.130-101-540) according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2022Quote: CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+isolation kit (Miltenyi Biotec) using PBMCs from three donors as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Tregs were isolated from peripheral blood mononuclear cells (PBMCs) by negative selection of CD4 (Miltenyi Biotec, Bergisch Gladbach, Germany) followed by positive CD25 selection (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... brain cells were isolated using a 30%/80% Percoll gradient after homogenizing tissue on a gentleMACS dissociator (Miltenyi Biotec). Single cell suspensions were then incubated with purified anti-CD16/32 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: For single-cell sequencing experiments samples were additionally run on a gentleMACS™ Dissociator (Miltenyi Biotec, #130-093-235) with the custom program h_skin_01 (Duration ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in TMRE buffer and subjected to flow cytometry on a MACSQuant Analyzer (Miltenyi Biotec). For mitochondrial superoxide measurements ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... the unbound complexes were washed and the cells processed through magnetic columns (Miltenyi Biotec, Catalog No. 130-042-401) following manufacturer instruction ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... synchronisation at trophozoite or schizont stage was performed by utilizing magnetic activated cell sorting (Miltenyi Biotec MACS LD columns).
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bioRxiv - Immunology 2024Quote: ... blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207 ...
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bioRxiv - Cancer Biology 2024Quote: ... Depletion of CD45+ cells in NSCLC samples was performed using CD45 MicroBeads (Miltenyi Biotec, cat. no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... The following antibodies were used for cell surface staining: IgA (clone IS11-8E10) (Miltenyi Biotec, cat#: 130-114-002), IgD (clone IA6-2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Human CD34+ cells were enriched as previously described on a CliniMACS Prodigy according to the manufacturer’s instructions (Miltenyi Biotec). Nonhuman primate CD34+ cells were harvested ...
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bioRxiv - Cancer Biology 2022Quote: ... and dead cell removal following the manufacturer’s protocols (Miltenyi Biotec, 130-095-929, 130-104-694, 130-090-101). Suspensions of single cells were processed on a Fluidigm C1 instrument using a C1 Single-Cell mRNA Seq HT IFC and Reagent Kit v2 (Fluidigm ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: hCD8+ T cells were isolated from commercially available sources (buffy coats from ZenBio Inc. and leukopaks from Miltenyi Biotec) using magnetic separation (positive selection ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were isolated from whole blood by hypotonic lysis of erythrocytes using Red Blood Cell Lysis Solution (Miltenyi Biotec) followed by ice cold PBS/1% BSA/2mM EDTA washes ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Immunology 2023Quote: ... Further purification of immune cells was done by magnetic enrichment of the CD45+ fraction (130-052-301, Miltenyi Biotec). All cell suspensions were kept in PEB buffer (0.5% BSA and 2 mM EDTA in PBS).
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bioRxiv - Neuroscience 2023Quote: ... Analyses of the labeled cells were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). The proportion of eGFP+ cells that co-labelled with CD11b+CD45+ was assessed and compared between groups using MACSQuantify v2.13.0 software.
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cells were collected and incubated with CD45 magnetic microbeads (130-045-801; Miltenyi Biotec, Bergisch Gladbach, Germany) for 30 minutes at 4℃ in the dark ...
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Neuroscience 2023Quote: ... Analysis for plasma- and cell line-derived EV samples was performed using the MACSQuant® Analyzer 16 (Miltenyi Biotec) and the corresponding software MACSQuantify™ (Version 2.13.3) ...
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bioRxiv - Neuroscience 2023Quote: Cells were isolated from the adult brain using the Adult Brain Dissociation kit (Miltenyi Biotec, cat # 130-107-677) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: Primary bone marrow-derived white blood cells were sorted for CD34 positivity using a CD34 MicroBead Kit (Miltenyi Biotec) and cultured for 10 days as detailed above (primary cultures ...
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bioRxiv - Neuroscience 2023Quote: ... and processed to create a single-cell suspension using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) supplemented with Actinomycin D (#A1410 ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cell concentrates were enriched in CD34+ HSPCs using a CliniMACS Plus instrument (Miltenyi Biotec, Gaithersburg, MD, USA) and cryopreserved prior to use ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Genetics 2023Quote: ... and enriched for iPSC-RPE via magnetically activated cell sorting using anti-CD24 and anti-CD56 antibodies (Miltenyi Biotec). iPSC-RPE cells in the negative cell fraction were seeded on the desired surface pre-coated with Laminin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
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bioRxiv - Cancer Biology 2023Quote: ... after which CD34+ stem cells were isolated by MACS sorting using CD34 MACS microbeads (Miltenyi Biotec, Leiden, The Netherlands) following manufacturers recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated for 10 min with 25 µl of FcR Blocking Reagent (Miltenyi Biotec 130-059-901) (pre-diluted 1:50 with FACS buffer (PBS ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes of P2-TCRα retrogenic mice were enriched for CD4+ cells using CD4 microbeads (Miltenyi Biotec, 130-117-043). Teffs (≤ 140,000 cells per mouse ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor samples were dissociated into single cells using GentleMACS tissue dissociator and its kit (Miltenyi Biotec, Gladbach, Germany). The tumor samples were cut into small pieces ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspension was used for the isolation of neurons using the Adult Neuronal Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads (Miltenyi Biotec, Ref#130-090-855) with auto MACS pro (Miltenyi Biotec) ...