Labshake search
Citations for NanoTemper :
551 - 600 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Mixtures were loaded into premium capillaries (Monolith Capillaries, NanoTemper Technologies). Thermophoresis was measured at 21°C for 15 sec with 50% LED power and 100% (auto-detect ...
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bioRxiv - Microbiology 2023Quote: ... For nanoDSF (Nanotemper), both ComFB and SbtB proteins were diluted in the ITC buffer and used at 1.5 mg/mL concentration with or without 0.5 mM c-di-AMP ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity Analysis software (NanoTemper Technologies). To ensure the accuracy and reliability of the results ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability analysis was conducted using the Tycho NT.6 (NanoTemper Technologies). SIDT1ECD and SIDT2ECD were diluted to a final concentration of 0.25 mg/mL at different pH values (pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: Protein Labeling Kit RED-maleimide 2nd Generation (cat# MO-L014; NanoTemper Technologies GmbH) was used for covalent labeling of LC3A cystine residues ...
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bioRxiv - Biochemistry 2023Quote: Thermal unfolding of SpyCatcher mutants was measured on a Prometheus nanoDSF device (Nanotemper) from 20°C to 90°C with a ramp rate of 2°C/minute.
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bioRxiv - Biophysics 2023Quote: ... Standard glass capillaries (NanoTemper Technologies) were utilized for the measurements ...
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bioRxiv - Biochemistry 2023Quote: ... 115 Pico (NanoTemper Technologies) at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... The interaction of NRP-1 structural variants with FOL-peptides was measured using a Monolith NT.115 [NT.115Pico/NT.LabelFree] instrument (NanoTemper Technologies, Munich, Germany). For more details see supplementary materials and methods.
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bioRxiv - Biochemistry 2023Quote: ... Data were recorded using the PR.ThermoControl v2.3.1 software and analyzed with the PR.StabilityAnalysis v1.1 software (Nanotemper). Melting temperatures were determined as the inflection points of the curves representing the ratio of fluorescence intensity at the two wavelengths (350nm/330nm ...
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bioRxiv - Biochemistry 2023Quote: ... Ten microliters of proteins were loaded into a capillary (PR-C002, Nanotemper) and thermal denaturation was recorded using a 1 °C/min ramp from 20 to 95 °C ...
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bioRxiv - Biochemistry 2023Quote: Thermal denaturation was analyzed by Nano-DSF using a Prometheus NT.48 instrument (Nanotemper). Mini-procollagens were diluted to a final concentration of 150 µg/mL in 20 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were loaded into the Monolith NT.115 device (NanoTemper) and MST was measured using 40% (RED ...
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bioRxiv - Neuroscience 2023Quote: ... Data were processed using the manufactuer’s software (MO.affinity 2.3, NanoTemper) following published protocols39 ...
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bioRxiv - Biochemistry 2023Quote: ... Microscale thermophoresis data were collected using a Monolith NT.115 instrument (NanoTemper). All experiments were performed using the red fluorescence laser (λexcitation = 600–640 nm ...
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bioRxiv - Immunology 2023Quote: ... were fluorescently labeled using His-Tag Labeling Kit RED-Tris-NTA 2nd Generation (NanoTemper, Cat# MO-L018). The proteins (at fixed concentration ...
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bioRxiv - Immunology 2023Quote: ... MST was run in Monolith NT.115 Instrument (NanoTemper) and analyzed with MO Affinity Analysis software ...
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bioRxiv - Immunology 2023Quote: ... and samples were loaded in Monolith capillaries (NanoTemper, Cat# MO-K022). MST was run in Monolith NT.115 Instrument (NanoTemper ...
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bioRxiv - Immunology 2023Quote: Thermal melting profiles of proteins were measured by differential scanning fluorimetry on a Prometheus NT.48 instrument (NanoTemper). Protein samples (0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... Protein samples (0.1 mg/mL) were loaded into glass capillaries (NanoTemper) and then subjected to a temperature gradient from 20 to 95°C at a heating rate of 1°C per min ...
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bioRxiv - Biophysics 2023Quote: ... The microscale thermophoresis (MST) traces of each sample were measured using a Nanotemper Monolith NT.115 (NanoTemper Technologies GmbH, Munich, Germany). The temperature-related fluorescence intensity change between the cold range (1 s before measurement started ...
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bioRxiv - Cancer Biology 2023Quote: ... Thermal unfolding was followed by differential scanning fluorimetry (DSF) by measuring intrinsic protein fluorescence using a Prometheus NT.48 (NanoTemper Technologies). Aliquots (10 μl ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM of the labeled ARC4 and 16 two-fold dilution series of SPIDER were loaded into sixteen premium capillaries (NanoTemper Technologies GmbH ...
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bioRxiv - Cell Biology 2023Quote: Microscale thermophoresis (MST) was performed on a NanoTemper Monolith NT.115 Pico instrument (NanoTemper Technologies GmbH) at 25°C using auto-detect Pico Red at 20% excitation power ...
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bioRxiv - Biophysics 2023Quote: ... and experiments were carried out using the NanoTemper Tycho NT.6 (NanoTemper Technologies, Germany). The temperature gradient was set to an increase of 30 °C/min from 35° C to 95° C ...
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bioRxiv - Biochemistry 2023Quote: We used the Tycho NT.6 (NanoTemper Technologies) for label-free thermal shift analysis ...
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bioRxiv - Biochemistry 2023Quote: ... using standard treated capillaries (Nanotemper). Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper ...
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bioRxiv - Biochemistry 2023Quote: Melting temperatures were assessed using Differential Scanning Fluorimetry (DSF) on a Prometheus Panta (NanoTemper Technologies GmbH, Munich, Germany). Purified G11iN3-S WT and G11iN3-S R96A were diluted in DSF buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The melting temperature was determined from the inflection point of the transition using the PR.ThermControl software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2023Quote: Thermal unfolding experiments were carried out on a Prometheus NT.48 instrument (NanoTemper Technologies) using 5 µM CerS6 solubilized in 0.003% LMNG and 0.0003% CHS ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were labelled in NHS labelling buffer using the Red NHS 2nd generation amine labelling kit (Nanotemper, MO-L011), then buffer exchanged into 10 mM potassium phosphate buffer ...
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bioRxiv - Biochemistry 2023Quote: Binding experiments were performed using microscale thermophoresis (MST).39 Experiments were performed on a Monolith NT.115 (NanoTemper) using 20% light-emitting diode power ...
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bioRxiv - Biochemistry 2023Quote: ... Purified GST fusion protein were fluorescently labeled using the Monolith series Protein Labeling Kit RED-NHS 2nd Generation (Nanotemper, MO-L011) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity Analysis software (NanoTemper) using the data on time 1.5 s ...
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bioRxiv - Biochemistry 2023Quote: MST experiments were performed on a Monolith NT.115 instrument (NanoTemper Technologies, Germany), using standard treated capillaries (Nanotemper) ...
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bioRxiv - Biochemistry 2023Quote: The MST experiments were conducted using a Monolith NT.115 instrument (NanoTemper Technologies). Purified SIDT1ECD and SIDT1ECD-Dimer were first exchanged into a labeling buffer containing 25 mM NaHCO3 pH 8.3 ...
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bioRxiv - Biochemistry 2023Quote: ... a 16-point affinity measurement was performed in duplicates using the Dianthus (NanoTemper Technologies GmbH, Germany) instrument ...
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bioRxiv - Biochemistry 2023Quote: ... Monolith protein labeling kit RED-NHS 2nd generation was purchased from NanoTemper Technologies (Munich ...
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bioRxiv - Biochemistry 2023Quote: ... Serial dilutions of TAM were made in 16 different capillaries containing 50 µM PhoR-GFP (such that the fluorescence in the NanoTemper™ Monolith is in the range of 1200-1400 U) ...
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bioRxiv - Biochemistry 2023Quote: ... and labeled with RED-NHS dye (NanoTemper). PTX was prepared in 25 mM HEPES (pH 7.4) ...
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bioRxiv - Biophysics 2023Quote: ... The experiments were conducted using a Monolith NT.115 Blue/Green instrument (NanoTemper Technologies) in three independent replicates ...
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bioRxiv - Microbiology 2023Quote: ... loaded into Monolith™ capillary tubes (NanoTemper Technologies), and analyzed using the Monolith NT.115 MiscroScale Thermophoresis instrument with MO.Control software (NanoTemper Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... Data were analyzed using MO.Affinity Analysis (NanoTemper Technologies).
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bioRxiv - Microbiology 2023Quote: ... and analyzed using the Monolith NT.115 MiscroScale Thermophoresis instrument with MO.Control software (NanoTemper Technologies). For binding assays in the presence of phosphatidylinositol phosphates (PIPs) ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein interaction was measured in standard capillaries (NanoTemper), 100% excitation power and medium or high MST power (IR-laser intensity) ...
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bioRxiv - Molecular Biology 2023Quote: ... 650 nm fluorescent dye using the Monolith NT RED-NHS lysine labeling kit according to manufacturer’s protocol (NanoTemper, Munich, Germany). RED labeled untagged sP36 at 100 nM and His6-GlnK1 in 16 different concentrations ranging from 7.5 µM to 0.23 nM or 10 nM of sP36-RED and AmtB1-His6 in 16 dilutions ranging from 12.6 µM to 0.38 nM (all concentrations based on monomeric molecular mass ...
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bioRxiv - Cancer Biology 2023Quote: Binding affinities were estimated by microscale thermophoresis using a Monolith NT.115 device (NanoTemper, Germany). We measured the thermophoretic movements of labeled molecules within a temperature gradient ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were fluorescently labeled using the RED-Maleimide 2nd Generation Protein Labeling Kit (NanoTemper Technologies GmbH, Germany) as recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: The capillaries were loaded into a Prometheus Panta instrument (NanoTemper Technologies). NanoDSF ...
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bioRxiv - Immunology 2023Quote: ... Version 2.3 (NanoTemper).