Labshake search
Citations for NanoTemper :
451 - 500 of 1200 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... were incubated with 0 mM to 50 mM GMP/GDP-mannose at room temperature for 15 minutes before measuring the Tm using a Tycho NT.6 instrument (NanoTemper Technologies, Germany). All experiments were done in two biological repeats and three technical repeats ...
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bioRxiv - Biochemistry 2023Quote: ... centrifuged for 5 min and loaded to standard capillaries (Nanotemper, #MO-K022). To investigate competitive effects of different ligands ...
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bioRxiv - Biochemistry 2023Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (NanoTemper Technologies). A red LED at 20% excitation power (red filter ...
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bioRxiv - Biochemistry 2023Quote: The thermal stability of wt-JDBD and mutant proteins was measured in the Prometheus NT.48 nanoDSF (nano differential scanning fluorescence) instrument (NanoTemper Technologies). For each protein ...
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bioRxiv - Systems Biology 2023Quote: Thermal unfolding of proteins was measured in absence and presence of metabolites by nano differential scanning fluorimetry (F350/F330) using a Prometheus NT.48 (NanoTemper) at 95% excitation power over a temperature gradient from 20 °C to 95 °C at an increase of 1 °C per minute ...
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bioRxiv - Biophysics 2023Quote: ... where the protein was labelled with Monolith Protein labelling kit RED-NHS (NanoTemper Technologies, Munich, Germany). In the binding assay ...
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bioRxiv - Biophysics 2023Quote: ... Experiments were carried out in a microMonolith NT.115 Blue/Red (NanoTemper Technologies) at 25 °C ...
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bioRxiv - Biophysics 2023Quote: ... pH 7.5 in premium capillaries (NanoTemper Technologies) at 40% MST power ...
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bioRxiv - Biophysics 2023Quote: ... sample were loaded into standard capillaries (Monolith NT Capillaries, Nanotemper Technologies). Before each measurement ...
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bioRxiv - Biophysics 2023Quote: ... Dose-response curve was plotted using the MO.Affinity Analysis software (Nanotemper Technologies) and outlier values were removed ...
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bioRxiv - Biophysics 2023Quote: ... Measurement was performed on a Monolith NT.115 MST apparatus (NanoTemper Technologies), by using a range excitation wavelength between 460 and 480 nm and a range emission wavelength measured between 515 and 530 nm ...
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bioRxiv - Immunology 2023Quote: ... Obtained MST traces were analyzed at an MST-on time of 1.5 s using the MO.Affinity Analysis version 2.2.4 (NanoTemper Technologies) for each of the three potential interaction pairs ...
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bioRxiv - Immunology 2023Quote: All MST experiments were performed on a Monolith NT.115 instrument with green/red filters (NanoTemper Technologies, Munich, Germany). MST and LED power were set at 40% and 60% ...
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bioRxiv - Developmental Biology 2023Quote: ... Recorded data were analyzed with MO.Affinity Analysis Software v2.3 (NanoTemper Technologies).
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bioRxiv - Cell Biology 2023Quote: ... a Monolith NT.115 Microscale Thermophoresis instrument (NanoTemper Technologies GmbH, München, Germany) was used ...
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bioRxiv - Cell Biology 2023Quote: ... The data were analyzed and fit using the MO.Affinity Analysis software (NanoTemper Technologies GmbH) to determine the Kd of FDX1 binding to LIAS ...
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bioRxiv - Cell Biology 2023Quote: ... Monolith RED-NHS 2nd generation protein labeling kit (NanoTemper Technologies GmbH) was used to label LIAS and ...
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bioRxiv - Cell Biology 2023Quote: ... Measurements were performed at 22 °C using medium MST power and 100% excitation power settings on the MO.control data collection software (NanoTemper Technologies GmbH). The data were analyzed and fit using the MO.Affinity Analysis software (NanoTemper Technologies GmbH ...
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bioRxiv - Developmental Biology 2023Quote: ... microscale thermophoresis (MST) assays were carried out by using a Monolish NT.115 (NanoTemper Technologies). His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper ...
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bioRxiv - Cell Biology 2023Quote: ... The Monolith NT.115 microscale thermophoresis instrument with premium coated capillaries (NanoTemper, Munchen, Germany) was used to measure binding curves ...
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bioRxiv - Cell Biology 2023Quote: ... Microscale thermophoresis was performed using a Monolith NT.115 (NanoTemper). Data were analyzed by MO Affinity Analysis software ...
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bioRxiv - Microbiology 2023Quote: ... thermal shift assays were performed with nanoDSF integrated in the Prometheus NT.48 (NanoTemper Technologies GmbH, Germany). Protein solutions of 75-100 µM of YopR WT ...
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bioRxiv - Biochemistry 2023Quote: ... microscale thermophoresis (MST) assays were performed on a Monolith X (Nanotemper Technologies) using standard capillaries (MO-K022 ...
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bioRxiv - Biochemistry 2023Quote: ... using a Prometheus NT.48 (NanoTemper, Germany) as previously described (33) ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 10 µL of each sample was loaded into Prometheus NT.48 Series nanoDSF high sensitivity capillaries (NanoTemper, Germany) and melting scans were performed using the Pr.ThermControl software (NanoTemper ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then briefly spun-down using a tabletop centrifuge and incubated in the dark at room temperature for one hour prior to being loaded into Monolith Standard Capillaries (NanoTemper, Germany) and microscale thermophoresis measurements were performed using the MO.Control Software with a medium laser power and fluorescence auto-detection ...
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bioRxiv - Biochemistry 2023Quote: ... Data were analyzed by MO.Control and MO.Affinity software (NanoTemper). The experiments were performed in triplicate.
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bioRxiv - Biochemistry 2023Quote: ... The measurements were performed on a Monolith NT.115 (NanoTemper) using standard capillaries at 25 °C with 80% MST power ...
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bioRxiv - Biochemistry 2023Quote: ... over 12 serial diluted Monolith NT.115 premium capillaries (NanoTemper) with 10 μl samples ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing varying concentrations of protein were loaded into glass capillaries and applied to the nanoDSF device Prometheus NT.48 (Nanotemper). The samples were heated from 20 to 90°C with a slope of 1.5°C/min and the unfolding of the proteins was observed by detecting shifts in the fluorescence at 330 and 350 nm ...
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bioRxiv - Bioengineering 2023Quote: ... and absorption at 330 and 350 nm was measured on a Prometheus NT.48 instrument (NanoTemper) in the temperature range 30-95°C with a temperature slope of 1.5°C/min ...
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bioRxiv - Immunology 2023Quote: ... Each sample was transferred into a capillary and measured from 35 °C to 95 °C using a Tycho N6T (Nanotemper). Data were analyzed in Prism (GraphPad Software).
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bioRxiv - Cell Biology 2023Quote: His-Tag labeling Kit RED-tris-NTA 2nd generation (MO-L018, Nanotemper) was used to label His-tagged Bax purchased from Creative BioMart (Catalog No ...
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bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis was performed using the Monolith Pico device (Nanotemper Technologies) as described previously (33) ...
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bioRxiv - Biochemistry 2023Quote: ... with the Prometheus device (Nanotemper Technologies). Measurements were carried out from 20°C to 95°C with a heating rate of 1°C/min.
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bioRxiv - Biochemistry 2023Quote: ... Each sample was measured with two technical and three independent biological replicates in standard capillaries (Nanotemper Technologies, Munich, Germany) with the Prometheus device (Nanotemper Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... then Plex nanoDSF Grade High Sensitivity Capillaries (NanoTemper) were filled with 10-µl protein sample ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged RALF proteins were labeled with red dyes with the His-Tag labelling kit RED-tris-NTA 2nd Generation (NanoTemper) according to the manufacturers’ recommendation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MST experiments were performed on a NanoTemper Monolith NT.115 (NanoTemper Technologies, Germany) with red filters at 25 °C and 20% MST power.
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bioRxiv - Pharmacology and Toxicology 2023Quote: Recombinant METTL16 MTD (residues 1-291) protein was labelled via His-tag using Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies GmbH, Germany). The labelled protein was diluted in MST buffer (10 mM Na-phosphate buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the samples were loaded into Monolith Premium Capillaries (NanoTemper Technologies GmbH, Germany). Measurements were done with Monolith NT.115 instrument (NanoTemper Technologies GmbH ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... MST data was analyzed in NT Analysis software (NanoTemper Technologies)
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and subjected to the MST measurement ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CK1γ2 was prepared at concentrations from 0.305 to 10000□nM and fluorescence dye from the Monolith His-tag Labeling kit RED-tris-NTA (NanoTemper Technologies) were added to concentrations of 80□nM and 5□nM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The MO.Affinity Analysis software v2.3 (NanoTemper Technologies GmbH, Germany) was used for Kd value calculations ...
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bioRxiv - Molecular Biology 2023Quote: ... Each binding experiment was performed in three independent runs and resulting MST data loaded to MO.Analysis (NanoTemper) software to estimate the Kd for MRE11 dimerization ...
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bioRxiv - Plant Biology 2023Quote: ... and mixed with different concentrations of barley total RNA (Phenol/Chloroform) before loading onto Monolith NT.115 (NanoTemper Technologies). Data were treated by the KD Fit function of the Nano Temper Analysis Software (version 1.5.3).
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bioRxiv - Plant Biology 2023Quote: The fluorescent dye NT-647 (MO-L001, NanoTemper Technologies) was used to label effector proteins GST or BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The measured data were fitted to the Hill equation using NT analysis software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2023Quote: ... Measurements were taken by using a Monolith NT.115 Instrument (NanoTemper Technologies) at 25 °C according to the manufacturer’s protocol ...