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Citations for NanoTemper :
501 - 550 of 1068 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... thermal melting was performed from 20 to 90°C using a PrometheusNT.48 DSF (Nanotemper) using high sensitivity capillaries ...
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bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
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bioRxiv - Biochemistry 2022Quote: ... and allowed to incubate in the dark for 30 min before loading into NT.115 premium treated capillaries (Nanotemper Technologies). A red LED at 30% excitation power (red filter ...
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bioRxiv - Cell Biology 2022Quote: Prometheus NT.48 instrument (NanoTemper Technologies) performed differential scanning fluorimetry (DSF ...
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bioRxiv - Cell Biology 2022Quote: ... 500mM Imidazole was loaded in nanoDSF grade standard capillaries (NanoTemper Technologies) and laser power optimised for signal ...
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bioRxiv - Biochemistry 2022Quote: ... Labeling of RIP2K1-300 was performed with Monolith Protein labeling kit RED-NHS 2nd generation (MO-L011, Nanotemper) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... tuberculosis DnaE1 was determined in the absence and presence of the ligand 6-thioinosine using a Tycho instrument (Nanotemper). 8 μM of M ...
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bioRxiv - Biochemistry 2022Quote: ... Experiments were performed in triplicates and data were fitted using the Kd model of the MO.Affinity Analysis software (Nanotemper Technologies). The dissociation constant Kd was obtained by plotting the bound fraction against the logarithm of ligand concentration.
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bioRxiv - Biochemistry 2022Quote: Thermal stability of GDAP1 variants was studied by nanoDSF using a Prometheus NT.48 instrument (NanoTemper), in SEC buffer ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... The equilibrium dissociation constant (Kd) was determined using the MO.Affinity Analysis software (NanoTemper Technologies, Germany) with the Kd fit function ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies, Germany). For Zn2+ titration ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: MST analysis was performed using Monolith NT.115 (NanoTemper Technologies, Germany) by staining His-tagged xtZnT8 with the RED-Tris-NTA 2nd Generation dye (NanoTemper Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... K17A and K18A were labeled using RED-NHS 2nd Generation (Amine Reactive) Protein Labeling Kit (Nanotemper Technologies). For all experiments ...
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bioRxiv - Biochemistry 2022Quote: ... Data analysis was performed with NTAffinityAnalysis software (NanoTemper Technologies) in which the binding isotherms were derived from the raw fluorescence data and then fitted with both NanoTemper software and GraphPad Prism to determine the KD using a non-linear regression method ...
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bioRxiv - Biochemistry 2022Quote: ... The HIS-tag fluorophores RED-tris-NTA 2nd Gen (NanoTemper MO-L018) and Atto-647 (Sigma 02175) ...
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bioRxiv - Biochemistry 2022Quote: ... All MST data was analyzed using MO.AffinityAnalysis software (Nanotemper) and fit using the time period +0.5 to +1.5 seconds after application of the IR laser ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were then loaded into standard capillaries and read on a NT.Automated (Nanotemper) using 10% excitation energy and “medium” MST power settings ...
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bioRxiv - Biochemistry 2022Quote: ... Binding curves were fitted and affinities (Kd) were calculated using MO.Affinity Analysis software (NanoTemper).
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bioRxiv - Biochemistry 2022Quote: Protein melting profiles using 5 μM of 4B7- or 1245-Db were measured with Tycho NT.6 (NanoTemper® Technologies GmbH). Fluorescence emission intensities was measured at I350 and I330 in triplicates and its ratio plotted as a function of temperature ...
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bioRxiv - Biochemistry 2022Quote: ... with a 30 °C/min increase rate (NanoTemper® Technologies GmbH). The Inflection temperature (Ti ...
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bioRxiv - Biophysics 2022Quote: The conformational stability of HBc/4M2e on the thermal denaturation analysis was performed by differential scanning fluorimetry without additional fluorescent labels by smoothly heating the sample in a scanning fluorometer (Prometheus NT.48, NanoTemper Technologies GmbH, Germany). HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml ...
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bioRxiv - Biophysics 2022Quote: ... HBc/4M2e samples in PBS buffer solution (at concentrations of 3 mg/ml) were collected in glass capillaries (Standard Capillaries Prometheus NT.48, NanoTemper), capillaries were placed on the platform and measurements were taken in the temperature range from 15°C to 95°C in increments of 1°C /min at a laser intensity of 50% ...
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bioRxiv - Biochemistry 2022Quote: ... equipped with back-reflection optics or with a Prometheus Panta (Nanotemper, Munich, Germany), equipped with back-reflection and DLS optics ...
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bioRxiv - Biochemistry 2022Quote: NanoDSF and light scattering of purified proteins were measured with a Prometheus NT.48 (Nanotemper, Munich, Germany), equipped with back-reflection optics or with a Prometheus Panta (Nanotemper ...
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bioRxiv - Cancer Biology 2022Quote: ... Microscale thermophoresis (MST) experiments were conducted in triplicate using Monolith automated capillaries (Cat no. MO-AK002, NanoTemper Technologies) with a Monolith NT Automated system (NanoTemper Technologies GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... fluorescent dye (Cat no. L008, NanoTemper Technologies) and applied at the final concentration of 50 nM in His-SPN SEC buffer having 0.05% Tween-20 ...
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bioRxiv - Biophysics 2022Quote: ... The samples were analyzed with a Monolith™ NT.115 pico device using MO.Control Software (Nanotemper). For PA and model membrane ...
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bioRxiv - Biophysics 2022Quote: EspB1-332 and free phospholipid or model membrane interaction assay was done using MST (Nanotemper)(43) ...
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bioRxiv - Biochemistry 2022Quote: ... followed by labeling with 30 μM Cysteine reactive dye (Monolith Protein Labelling Kit Red-Maleimide 2nd Generation, NanoTemper), and incubated in dark at Room Temperature (RT ...
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bioRxiv - Biochemistry 2022Quote: Thermal-shift assays of SARS-CoV-2 Mpro and its H172Y mutant were carried out using the nanoDSF method as implemented in the Prometheus NT.48 (NanoTemper Technologies). The nanoDSF method is based on the autofluorescence of tryptophan (and tyrosine ...
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bioRxiv - Biochemistry 2022Quote: ... The melting temperature (Tm) was calculated as the mid-point temperature of the melting curve using the PR.ThermControl software (NanoTemper Technologies).
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bioRxiv - Biochemistry 2022Quote: ... Then the proteins were loaded onto Prometheus NT.48 nanoDSF Grade Standard Capillaries (PR-C002, NanoTemper Technologies), the fluorescence signal was recorded under a temperature gradient ranging from 25 to 90°C (incremental steps of 0.5°C min-1) ...
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bioRxiv - Biochemistry 2022Quote: The amine residues of purified KsgA were fluorescently labeled with NHS red using the Protein Labeling Kit RED-NHS 2nd Generation (Cat # MO-L011 Nanotemper). The labeling reaction was performed according to the manufacturer’s protocol by mixing KsgA at a final concentration of 20 μM with a 3-fold molar excess of dye at room temperature for 30 minutes in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... The binding affinity measurements were carried out using the modified manufacturer’s protocol (Monolith NT.115pico, NanoTemper Technologies) in the Center for Macromolecular Interactions in Harvard Medical School (Boston ...
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bioRxiv - Biochemistry 2022Quote: ... MST experiments were recorded at 680 nm and processed by NanoTemper analysis 1.2.009 and Origin8G.
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bioRxiv - Biochemistry 2022Quote: ... using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). MST relies on binding-induced changes in thermophoretic mobility ...
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bioRxiv - Biochemistry 2022Quote: Microscale thermophoresis (MST) experiments were performed using a Monolith NT.115 (Nanotemper Technologies). Labeled purified Snf1 complex (0.144 µM) ...
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bioRxiv - Biochemistry 2022Quote: The thermal stability of SETX-HD WT and the R2136H variant was measured using the Prometheus NT.38 instrument (NanoTemper Technologies). NanoDSF grade capillaries were filled with protein samples at 1 mg/ml in protein dilution buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Microscale thermophoresis (MST) measurements were performed using the Monolith NT.115 microscale thermophoresis instrument (NanoTemper Technologies) at 25 ºC ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were loaded by capillary action into standard sensitivity capillaries and placed within a Prometheus Panta (Nanotemper). The instrument was allowed to stabilize at 20°C before measuring ...
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bioRxiv - Biochemistry 2022Quote: ... All reactions were incubated for 20 minutes at 25 ºC before loading into premium glass capillaries (NanoTemper Technologies). Microscale thermophoresis (MST ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were loaded to the capillaries (NanoTemper Technologies, catalog #: MO-K025) and measured using a Monolith NT.115 instrument with the settings of 40% LED/excitation and 40% MST power ...
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bioRxiv - Cell Biology 2022Quote: ... Monolith His-Tag Labeling Kit RED-tris-NTA 2nd Generation (NanoTemper Technologies, catalog #: MO-L018) was used to label recombinant His-α1-ERD (MyBioSource ...
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bioRxiv - Cell Biology 2022Quote: ... using a Monolith NT.115 instrument (NanoTemper Technologies Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... was mixed with 16 serial dilutions of strep-NHR-85 starting at 31.5 uM and loaded into microscale thermophoresis premium coated capillaries (NanoTemper Technologies). MST measurements were recorded at 25°C using 30% excitation power and 60% MST power ...
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bioRxiv - Developmental Biology 2022Quote: Binding assays of purified NHR23 or strep-NHR85 was measured using a Monolith NT.115 Pico running MO Control version 1.6 (NanoTemper Technologies). Assays were performed in 100 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... and to liposomes was assessed by MST using a Monolith NT.115 system (Nanotemper Technologies, Munich, Germany). Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments were performed in triplicates and data were fitted using the KD model of the MO.Affinity Analysis software (Nanotemper Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... Around 12 μL of protein complex at a concentration of 0.15 mg/mL in buffer B were applied into standard grade NanoDSF glass capillaries (Nanotemper). Capillaries were loaded into a Prometheus NT.48 device (Nanotemper ...