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Citations for Agilent :
1901 - 1950 of 2020 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The aqueous samples were resuspended in 35x the packed cell volume using 80:20 methanol:water and transferred to polypropylene mass spectrometry vials (Agilent, 5188-2788).
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were rinsed three times with phosphate-buffered saline and mounted with a fluorescence mounting medium (S3023; Dako, Agilent Technologies). Images were captured using a laser scanning microscope (BZ-9000 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed thrice with 0.1% (v/v) tween-20 / PBS and incubated in Serum Free Protein Block (DAKO, cat.#X0909) for 1h at 21°C ...
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bioRxiv - Biochemistry 2023Quote: ... A total of 100,000 cells were counted at each experimental point using a NovoCyte Flow cytometer (Agilent, Santa Clara, CA, USA). DHR-stained cells were detected using 488 nm for excitation and 530/30 nm for emission.
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bioRxiv - Cell Biology 2023Quote: Mitochondrial respiration (JO2) of intact cells were measured using an XF HS mini Analyser Extracellular Flux Analyzer (Seahorse Bioscience, Copenhagen, Denmark). L6 myoblasts were seeded and differentiated in Seahorse XFp culture plates coated with matrigel and assayed after incubation at 37°C without CO2 for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with DAPI (1 μg/ml) and mounted onto glass slides using DAKO Fluorescent Mounting Medium (Agilent Technologies S3023). Images were acquired using an Upright ZEISS AXIO Imager.M2 with Apotome 2.
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bioRxiv - Physiology 2024Quote: ... the cell pellet was resuspended in 500ul of warm MAS buffer solution [58] + 1nM Plasma Membrane Permeabilizer (Agilent Seahorse XF PMP) to permeabilize the cells ...
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bioRxiv - Biochemistry 2024Quote: ... pT7-7 α-syn FL plasmid (a gift from Hilal Lashuel, Addgene66) was transformed into BL21-Gold (DE3) competent Escherichia coli (E. coli) cells (Agilent Technologies) according to the manufacturer’s instructions and previous methodology44 ...
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bioRxiv - Neuroscience 2024Quote: Ten µg of mitochondrial protein in a volume of 30 µl were added to each well of a Seahorse XFe96/XF Pro Cell Culture Microplates (Agilent Technologies) coated with polyethylenimine (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: Mitochondrial respiratory function in live SH-SY5Y cells was measured using the Seahorse XFe96 Extracellular Flux Analyzer (Agilent Technologies, California, USA) via changes in the Oxygen Consumption Rate (OCR ...
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bioRxiv - Biophysics 2024Quote: OCR measurements were performed with the Agilent Seahorse XF Cell Mito Stress Test kit with an XF24 extracellular flux analyser (Seahorse Bioscience). XF24 plates were coated with either 23 µl of 100% Matrigel (soft ...
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bioRxiv - Neuroscience 2024Quote: ... except that the dissociated spinal cord cells were plated in 80 µL serum-containing plating medium (50% DMEM, 25 % HBSS and 25 % horse serum) at 80.0000 cells per well in Seahorse XFp Miniplates (Agilent, 103025-100). After two hours ...
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bioRxiv - Microbiology 2024Quote: ... Cell densities were determined in two technical replicates with a NovoCyte Flow Cytometer equipped with a NovoSampler Pro (Agilent, Waldbronn, Germany). Two times 200 µL of each sample were pipetted into a 96-well microtiter plate (Sarstedt ...
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bioRxiv - Biophysics 2021Quote: ... The pre-incubated LNPs (see above) were transferred to the cells using a Bravo liquid pipetting robot (Agilent Technologies, Santa Clara, US). All treatments were analysed in four technical replicates ...
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bioRxiv - Biophysics 2021Quote: Wild-type (WT) 3RS-and 4RL-Tau constructs were expressed in BL21 CodonPlus(DE3)-RP Escherichia coli cells (Stratagene, La Jolla, CA) and purified as previously described [17 ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA was extracted with Norgen single cell RNA purification kit (Norgen) and the quality was checked with a Bioanalyzer 2100 (Agilent, RIN >8.0). Ribosomic RNA was depleted with Ribo-Zero™ Gold Kit (Epicentre) ...
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bioRxiv - Cell Biology 2021Quote: Measurements of aerobic respiration and glycolysis were conducted with the Seahorse Bioscience XFe-96 bioanalyzer using the Seahorse XF Cell Mito Stress Test Kit (Agilent #103015-100). Cells were seeded on XF96 cell culture microplates (Agilent #102416-100 ...
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bioRxiv - Cell Biology 2019Quote: Cell mitochondrial oxygen consumption rates (OCRs) were assayed in 96-well plates by using a XF Cell Mito Stress Test Kit (Cat. #: 101706100, Seahorse Bioscience, U.S.) on the XFe96 Extracellular Flux Analyzer (Seahorse Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with prediluted antibody polyclonal Guinea Pig anti-Human Insulin (cat. IR002 - Agilent Technologies, Santa Clara, CA, USA) for 1h at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were co-transfected with 100ng pCAG-Lgr6 overexpression plasmid (pLgr6) or pBluescript II KS+ (pBKS) (Agilent Technologies, Santa Clara, CA, USA) as a control ...
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bioRxiv - Developmental Biology 2019Quote: ... OCR and ECAR parameters were measured using the Seahorse XFp Cell Energy Phenotype Test Kit™ (Agilent Biotechnology, Cat No. 103275-100). Metabolic stress was induced by simultaneous treatment with 1μm Oligomycin and 0.125µM Carbonyl cyanide p-[trifluoromethoxy]-phenyl-hydrazone (FCCP).
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bioRxiv - Biochemistry 2020Quote: The oxygen consumption rate (OCR) of LPO-exposed HLCs was measured using the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100) on a Seahorse XF Analyser (Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... to extract total RNA cells from the various experimental conditions and tested for RNA quality using a Bioanalyzer (Agilent, Santa Clara, CA). Contaminating genomic DNA was removed using Turbo DNA free from Ambion ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial respiration and glycolysis were assessed using Seahorse extracellular flux analyzer (XFe96) and Seahorse XF Cell Mito Stress Test Kit (Agilent Technologies, USA). Cells were seeded in 96-well plates (2 × 104 cells/well) ...
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bioRxiv - Cell Biology 2020Quote: His-tagged Ran in the pET28 vector (a gift from Dr. Günter Blobel) was transformed into BL21 (DE3) RIL-competent cells (Stratagene, St. Louis, MO). Expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA libraries were assessed for quality and quantification according to the Chromium Single Cell 3’ Reagent kit v3 user guide using the High Sensitivity D5000 ScreenTape (Agilent 5067-5592) and 2200 TapeStation Controller software (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... 150,000 cells (activated with anti-CD3 and anti-CD28 antibodies 48 hours prior) were plated into individual wells of a poly-D-lysine coated seahorse cell culture microplate (Agilent; 101085-004). T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM ...
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bioRxiv - Cell Biology 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of NSPCs was measured using the Seahorse XF Cell Energy Phenotype Test Kit (Agilent, # 103325-100), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... slices were then incubated with anti-cleaved caspase 3 antibody or anti-Ki67 antibody (Cell Signalling) and further processed with secondary antibody (LSAB2 horseradish peroxidase kit; Dako, Copenhagen, Denmark).
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bioRxiv - Developmental Biology 2021Quote: For chimera RNA sequencing we first FACS sorted GFP-expressing 500 cells and used microcapillary electrophoresis on Agilent 2100 Bioanalyzer with RNA Pico 6000 kit (Agilent, 5067-1513) to analyze RNA quality (RIN values > 8) ...
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bioRxiv - Biochemistry 2021Quote: ... fluorescence of labeled and unstained control cells was quantified with an ACEA NovoCyte benchtop flow cytometer (Agilent Technologies, Santa Clara, CA, USA). After applying a cut-off of 7.5 × 105 to the forward scatter ...
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bioRxiv - Immunology 2019Quote: Individual subunits (Strep-Tactin and dead streptavidin-SpyCatcher or dead streptavidin and Strep-Tactin-SpyCatcherΔ) were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies # 230280) and refolded from inclusion bodies using a modified version of the protocol previously described by Howarth and Ting43 ...
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bioRxiv - Neuroscience 2022Quote: Co-transfection of pAAV-CAG-DIO-(hCAR)off-(GtACR2Kv2.1-MuGFP)on-W3SL with pDPI and pDPII plasmids (Grimm et al., 2003) into AAV293 cells (Agilent Technologies, CA, USA) preceded harvesting and iodixanol gradient purification (as described by (Zolotukhin et al. ...
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bioRxiv - Plant Biology 2022Quote: ... USA) equipped with a Cobalt cell (Teledyne Photon Machines) and connected to an Inductively Coupled Mass Spectrometer (model 8900, Agilent Technologies, USA). Analysis was done with the following settings ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 1 hour followed by vehicle or TGF-β (5 ng/ml) and 3000 cells were seeded into Seahorse assay microplates (Agilent Technologies). After 24h of incubation ...
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bioRxiv - Cell Biology 2022Quote: ... Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in MIN6 cells using an XF24 Analyzer (Seahorse Bioscience, MA, USA).
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bioRxiv - Neuroscience 2024Quote: ... OCR and ECAR were measured as follows: cell culture media was removed and 80µl of XF Media (Agilent Technologies; Cat #: 103334-100) containing no pH buffers and supplemented with 5.5 mM glucose ...
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bioRxiv - Neuroscience 2024Quote: ... 10K HMC3 microglia per well were seeded in 80µl of a Agilent Seahorse XF96 Cell Culture Microplate (Agilent Technologies; Cat #: 101085-004) and incubated overnight ...
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bioRxiv - Systems Biology 2024Quote: Media to be used for cultivating cells for intracellular metal quantification with ICP-MS was through a PVDF membrane plate (Agilent 200931-100) into fresh deep-well (2mL ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Neuroscience 2024Quote: OCR assay: Cells were washed and incubated for 1 hour with 180 μL assay medium [in mmol/L: 5 glucose (Agilent #103577-100), 1 pyruvate (Agilent #103578-100) ...
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bioRxiv - Cancer Biology 2024Quote: ... Drug was then washed out and 1x105 cells plated in 5 replicate wells for immediate analysis using the Mito Stress Test Kit (Agilent, 103015-100) according to the manufacturer’s instructions and using the following drug concentrations ...
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bioRxiv - Cell Biology 2024Quote: MitoSnap+ and MitoSnap-cells were purified by FACS and their oxygen consumption rates (OCR) were measured using a XF96 MitoStress Test (Seahorse Agilent, 103015-100). Activated CD8+ T cells were washed in RPMI 1640 without sodium bicarbonate ...
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bioRxiv - Immunology 2023Quote: ... following standard protocol and plated at a concentration of 2×105 cells per well of a seahorse XFe96 assay plate in seahorse XF DMEM media (Agilent; 103575-100) supplemented with 1mM pyruvate ...
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bioRxiv - Physiology 2023Quote: ... For β-cell proliferation analysis in P1 pups every other section was labeled with guinea pig anti-insulin (1:200; DAKO A0564) and mouse anti- BrdU (1:500 Sigma B2531 ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Biophysics 2023Quote: ... The cells were fixed with 3% formalin 3 days after the transfection and analyzed with the ACEA Quanteon (Agilent NovoExpresss Version 1.5.0), as described (22 ...
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bioRxiv - Synthetic Biology 2024Quote: For FI-MS cell extracts were directly injected into an Agilent 6546 Series quadrupole time-of-flight mass spectrometer (Agilent Technologies, USA) as described previously9,10 ...
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bioRxiv - Immunology 2024Quote: ... The mitochondrial function of macrophages and amastigotes was analyzed by the Agilent Seahorse XF Cell Mito Stress Test Kit (Agilent, 103015-100). The glycolytic function of infected macrophage was analyzed by the Agilent Seahorse XF Glycolytic Stress Test Kit (Agilent ...