Labshake search
Citations for Agilent :
1551 - 1600 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... The pETM11-Sumo3-Tn5E54K,L372P plasmid was obtained from EMBL-Heidelberg and transformed into Escherichia coli BL21 (DE3) Gold cells (Agilent). Bacterial cultures were grown at 30◦C to a density of OD600=0.6-0.8 and protein expression was induced with 0.5 mM isopropyl-β-D-thiogalactoside (IPTG) ...
-
bioRxiv - Neuroscience 2021Quote: ... RNA from cultured and transfected DRG cells was purified using the Absolutely RNA Nanoprep Kit (Agilent Tech., Santa Clara, CA). RNA quantity was measured using a NanoDrop 1000 (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... fixed paraffin-embedded and analysis of alpha and beta cell proliferation was performed in sections immunolabeled with anti-insulin (A5064, Agilent, Santa Clara ...
-
bioRxiv - Cancer Biology 2020Quote: Microarray was performed on in vitro cultures of dMMR and CIN MC38 CRC cells using the SurePrint G3 Mouse Gene Expression v2 Microarray (8×60K) (Agilent) system ...
-
bioRxiv - Immunology 2021Quote: ... the utility plate without cartridge was unloaded and the cell plate was loaded into the XF24 analyser (Seahorse, Agilent Technologies). After equilibration ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS and incubated with a fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse IgG (Dako; 1:200). Cell nuclei were stained with 4’ ...
-
bioRxiv - Plant Biology 2022Quote: ... The concentration of copper in processed yeast cells was analyzed by inductively coupled plasma mass spectroscopy (ICP-MS; Agilent 7500).
-
bioRxiv - Immunology 2019Quote: ... maximal and reserve capacities and non-mitochondrial respiration were assessed using the Seahorse XFp Cell Mito Stress Test Kit (Agilent).
-
bioRxiv - Biophysics 2019Quote: ... anti-surfactant C protein antibody to target membrane antigen secreted from airway type 2 epithelial cells in alveoli or ii) anti-thyroid transcription factor-1 (TTF-1) antibody (Dako Agilent Products ...
-
bioRxiv - Biophysics 2019Quote: ... anti-surfactant C protein antibody to target membrane antigen secreted from airway type 2 epithelial cells in alveoli or ii) anti-thyroid transcription factor-1 (TTF-1) antibody (Dako Agilent Products ...
-
bioRxiv - Immunology 2019Quote: ... 5×105 CD8+ T-cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose ...
-
bioRxiv - Cancer Biology 2020Quote: ... the cell media was replaced with the assay media and ran using the Seahorse XF Cell Mito Stress Test and ATP Rate Assay on a Seahorse XFe96/XF96 analyzer (Agilent). Hoechst 33342 staining (ThermoFisher ...
-
bioRxiv - Physiology 2019Quote: The oxygen consumption rate (OCR) of HL-1 cardiomyocytes was measured using the XFp Cell Mito Stress Test by Agilent Seahorse24 ...
-
bioRxiv - Physiology 2021Quote: ... cells were subjected to a Seahorse XF Mito Stress Test using the manufacturer’s instructions for timing (Agilent, Santa Clara, CA), as described (Lassiter et al. ...
-
bioRxiv - Systems Biology 2020Quote: ... lymphatic tissue was cut into 1-mm pieces with a sterile scalpel and disaggregated mechanically into a single-cell suspension using the Medimachine (DAKO) equipped with sterile cartridges holding rotating knives (Medicons ...
-
bioRxiv - Microbiology 2021Quote: All viral stocks were generated in Vero cells and tested negative for Mycoplasma contamination using a MycoSensor PCR Assay Kit (Agilent). Detailed passage histories of the ZIKV isolates used in this study were previously described (56) ...
-
bioRxiv - Microbiology 2021Quote: The CAN97-83 HMPV P construct was cloned into the plasmid pET 302/NT-His between the cleavage sites EcoRI and XhoI and expressed in BL21(DE3) CodonPlus RIL cells (Agilent) overnight at 37 °C in terrific broth (TB ...
-
bioRxiv - Microbiology 2019Quote: ... the circular glass cover-slips containing the cell monolayers were mounted on glass slides using an anti-fade mounting solution (DakoCytomation) and visualized using an epifluorescence microscope (Olympus BX60) ...
-
bioRxiv - Biochemistry 2021Quote: ... All reactions were carried out using a TGradient (Biometra) thermocycler and resulting mutant plasmids transformed into electro competent BL21-Gold (DE3) cells (Agilent). The integrity of all constructs and PK mutations were confirmed by sequencing (Genewiz).
-
bioRxiv - Biochemistry 2021Quote: ... a 15-cm dish of cells was washed with PBS and UV-irradiated (120mJ/cm² at 254 nm) using a UV Stratalinker 2400 (Stratagene). Next ...
-
bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
-
bioRxiv - Neuroscience 2021Quote: Flow cytometry experiments were performed on at least 200,000 cells for each sample by using Novocyte 3000 (Agilent Technologies, Inc) equipped with 488 nm lasers ...
-
bioRxiv - Biochemistry 2021Quote: ... The product of each mutagenesis reaction was transformed into XL1-Blue Electroporation-Competent Cells (Agilent Technologies, Santa Clara, CA, USA) and serial dilutions were plated to determine the total number of colonies ...
-
bioRxiv - Genomics 2020Quote: BG4-encoding plasmid (kindly provided by Professor Shankar Balasubramanian, University of Cambridge, UK) was transformed into BL21(DE3) competent cells (Stratagene) which were cultured in TY medium (1.6% tryptone peptone ...
-
bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
-
bioRxiv - Cell Biology 2021Quote: The oxygen consumption rate (OCR) in MIN6 cells were monitored by an Extracellular Flux Analyzer (XFe96, Agilent Tchnologies, CA, USA). 3×104 cells (wildtype and TMEM24 KO ...
-
bioRxiv - Genomics 2021Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
-
bioRxiv - Cell Biology 2021Quote: ... Total RNA from cells was quantified by nanodrop and total RNA from exosomes was quantified using an RNA bioanalyzer (Agilent). Typically ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed thrice in PBS and incubated for 30 min with HRP-conjugated rabbit anti-mouse antibody (DaKo, P0161) diluted 1:5000 in blocking buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... and spare respirometry capacity were calculated using the Seahorse XF Cell Mito Stress Test Report Generator via Wave Software (Agilent) normalized to total protein levels.
-
bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
-
bioRxiv - Cell Biology 2022Quote: Optimal seeding density and FCCP concentration without toxicity response were tested before starting the Seahorse XF Cell Mito Stress Test assay (Agilent). A cell density of 4×103 cells per well and a FCCP concentration of 2 µM was determined and used for the following assays ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA from cells was quantified by nanodrop and total RNA from exosomes was quantified using an RNA bioanalyzer (Agilent). Typically ...
-
bioRxiv - Genomics 2022Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
-
bioRxiv - Immunology 2022Quote: All the tumor cells used in the paper tested negative for mycoplasma (MycoAlert®, Lonza and MycoSensor qPCR®, Agilent). The metastatic melanoma cells were cultured in RPMI 1640 (Corning ...
-
bioRxiv - Immunology 2022Quote: ... and probed with 8ng/mL anti-calnexin antibody (Cat. No. 2433S, Cell Signalling) overnight followed by 25ng/mL goat anti-rabbit -HRP (Cat. No. P0448, Agilent) secondary antibody or with 600ng/mL anti-GAPDH directly conjugated with HRP (Cat ...
-
bioRxiv - Physiology 2022Quote: ... were assessed by counting positive-stained cells in three different fields per section and were expressed as the percentage of positive cells of the total mature VSMCs (smooth muscle actin αSMA, M 0851 clone 1A4, DAKO). Collagen staining was assessed using Gomori’s 1-step trichrome stain (ab150686 ...
-
bioRxiv - Molecular Biology 2022Quote: ... live cell imaging lines were generated using a U-2 OS cell line harboring a Flp-In site and stably expressing a ponasterone A inducible system (Agilent), a gift from Robert Singer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were stained with CellROX reagents (Green) and Hoechst 33342 was used as nuclear stain and viewed in Cytation cell imaging multimode reader (Agilent) immediately ...
-
bioRxiv - Neuroscience 2023Quote: ... constructs cloned in AAV-hSyn vectors were transfected with RC/DJ and adenovirus-helper plasmid into the AAV-293 cells (#240073, Agilent) using calcium phosphate precipitation method ...
-
bioRxiv - Neuroscience 2022Quote: Mitochondrial and glycolytic function was analyzed with the Seahorse Cell Mito Stress Test and Seahorse Real-Time ATP Rate Assay on a Seahorse XFe24 Extracellular Flux Analyzer (Agilent). Neurons were treated on day 11 of culture with 500 μL of fresh neuronal media plus 500 μL of astrocytic conditioned media of varying treatment ...
-
bioRxiv - Plant Biology 2022Quote: Plasmids were transformed and expressed in the Escherichia coli strain BL-21 (DE3) RIL cells (Agilent, Santa Clara, CA, USA). For protein expressions ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were seeded at a density of 40,000 cells per well in a Seahorse 96-well plate (Seahorse XFe96, Agilent Technologies). Cells were incubated with 10nM GIP or vehicle for 3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) were measured in cell lines using a Seahorse XFe96 Analyzer (Agilent).
-
bioRxiv - Cell Biology 2022Quote: ... on a OCR and ECAR were measured using a Seahorse XF Cell Mito Stress Test kit (103015-100, Agilent Technologies) as described above.
-
bioRxiv - Neuroscience 2022Quote: Endogenous OCR was measured in adherent SH-SY5Y cells using a Seahorse XF-24 Analyzer (Seahorse Bioscience, Billerica, MA, USA) following the manufacturer’s protocol with minor modifications (63) ...
-
bioRxiv - Bioengineering 2023Quote: ... The luminescence signal which represents the amount of ATP in viable cells was measured with a microtiter well plate reader (BioTek Cytation 5, Agilent).
-
bioRxiv - Neuroscience 2023Quote: ... Primary fibroblasts plated in 96-well assay plate at a density of 40,000 cells/well were cultured overnight and then equilibrated with Seahorse XF base medium (Agilent Technologies) supplemented with glucose ...
-
bioRxiv - Biochemistry 2022Quote: ... The sensor plate was calibrated in the machine prior to loading cells and running a mitochondrial stress test using a Seahorse XFe96 analyser (Agilent). Following assays ...
-
bioRxiv - Systems Biology 2022Quote: ... Adherent NSCs were infected with retro-Cre-Tomato retrovirus (5μl of virus was used to infect 3x105 cells) or nucleofected with expression constructs according to instructions of the Amaxa nucleofection kit (Agilent) [28] ...