Labshake search
Citations for Agilent :
1701 - 1750 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: Life cell metabolic analyses were performed measuring extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) using Seahorse XFp Analyzer (Agilent Technologies). 4×105 cells per well were seeded and incubated for 3 days ...
-
bioRxiv - Cell Biology 2022Quote: ... were isolated by flow cytometric cell sorting into 96-well plates and preamplification-free single-cell whole genome sequencing libraries prepared using a Bravo automated liquid handling platform (Agilent Technologies) as previously described31,32 ...
-
bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
-
bioRxiv - Cancer Biology 2020Quote: Ribonucleic acid was isolated from cells in indicated experimental conditions using a Qiagen miRNAeasy kit (Valencia, CA) and measured on an Agilent Bioanalyzer (Agilent Technologies). Illumina Novaseq 6000 libraries were prepared and sequenced by Novogene (CA ...
-
bioRxiv - Biochemistry 2019Quote: Oxygen consumption rates (OCR) and extracellular acidification rates (ECAR) of muscle stem cells were measured using an XF24 Bio analyzer (Seahorse Bioscience), as described previously (Forni et al ...
-
bioRxiv - Biochemistry 2019Quote: ... the gene coding for the kinse domain of the Elk receptor was amplified from TKB1 cells (Agilent Technologies, Santa Clara, CA) and cloned with a C-terminal HA-tag between NdeI and EcoRV restriction sites within the second open reading frame of the pCDF-Duet vector ...
-
bioRxiv - Cell Biology 2019Quote: The intact cellular OCR of LonP1 stable knockdown and control H9c2 cells was measured using a Seahorse XF-96 Extracellular Flux Analyzer (Seahorse Bioscience) as described previously (40) ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were incubated at 37°C and the medium was replaced with 180 μl XF assay medium (#102365-100, Seahorse Bioscience) containing 10 mM glucose ...
-
bioRxiv - Cell Biology 2020Quote: ... parental HCT116 cells and HCT116 cells with SMYD5 overexpression (OE) or knockout (KO) were seeded in 96-well plate (in triplicates) (#101085-004, Seahorse Bioscience) one day before measurement ...
-
bioRxiv - Physiology 2020Quote: ... Rod shaped adult cardiomyocytes were collected by settling down of cells and plated in 24 well plates for XF24e Extracellular Flux Analyzer analysis (Seahorse Bioscience) or on glass coverslips for confocal imaging.
-
bioRxiv - Neuroscience 2020Quote: ... the original plasmid was digested with DpnI restriction enzyme and the remaining mutagenized plasmid was transformed into XL10-Gold® ultracompetent cells (Agilent). Integrity of the insert sequence and introduction of the point mutation were confirmed by Sanger sequencing of the isolated plasmid DNA.
-
bioRxiv - Synthetic Biology 2021Quote: ... The molarity of the single cell libraries was calculated based on their library sizes as measured using a bioanalyzer (Agilent Technologies) and using the KAPA qPCR quantification (KAPA ...
-
bioRxiv - Neuroscience 2019Quote: ... astrocytes (5×104 cells/well) were plated in a Seahorse XFe 24 microplate (Seahorse Biosciences/Agilent Technologies, Santa Clara, CA, USA) coated with 0.05mg/ml PDL and treated with 1 μM cisplatin or vehicle for 24h ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were washed four times with PBS containing Ca2+ and Mg2+ and mounted in a fluorescent mounting medium (DAKO, CA, USA). Finally ...
-
bioRxiv - Cancer Biology 2020Quote: ... Real-time oxygen consumption rate (OCR) of these cells was measured using the Seahorse Extracellular Flux (XFe-96) analyzer (Agilent Technologies). Protein concentration of cells in each well was determined ...
-
bioRxiv - Cell Biology 2021Quote: The bioenergetic profile of BMDMs were assessed by measuring the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) using a XF cell Mito Stress Test kit and XF glycolysis stress kit (Agilent Technologies) as per manufacturer’s instructions using a Seahorse XF extracellular flux analyzer (Agilent technologies ...
-
bioRxiv - Physiology 2020Quote: ... and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on XF96 cell culture microplates (Cat# 101085-004, Agilent Technologies) coated with poly-D-lysine (Cat# P6407 ...
-
Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... Total RNA from all other cell lines was used to prepare cDNA sequencing libraries using the SureSelect Strand-Specific RNA library preparation kit (Agilent Technologies) after the polyA selection step ...
-
bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
-
bioRxiv - Cancer Biology 2020Quote: ... OVCAR5 shctrl and shFZD7 cell lines were seeded in Seahorse 96-well microplate (Agilent, Cat#102416-100, Santa Clara, CA, USA) at a density of 10-80K per well.
-
bioRxiv - Developmental Biology 2021Quote: PSM cells were dissociated on day 2 of differentiation and reseeded onto fibronectin-coated Seahorse plates (Agilent cat. no. 101085-004) at a density of 7 x 105 cells per cm2 in Seahorse XF DMEM (Agilent cat ...
-
bioRxiv - Developmental Biology 2021Quote: ... RNA extracted from FACS isolated cells was quantified and assessed for quality using the RNA 6000 Pico Kit (Agilent – 5067-1513) and an Agilent 2100 Bioanalyzer ...
-
bioRxiv - Immunology 2021Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured in B cells using a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies) with kits from Agilent (Santa Clara ...
-
bioRxiv - Biophysics 2020Quote: ... flow cytometry analysis was performed by using the trophoblast-specific epithelial cell marker cytokeratin 7 (Maldonado-Estrada et al., 2004) (anti-cytokeratin 7, Dako, Switzerland). Vimentin (anti-vimentin ...
-
bioRxiv - Cancer Biology 2021Quote: ... we next prepared the sorted nuclei for pre-amplification-free mini-bulk/single-cell whole genome sequencing using a Bravo liquid handler platform (Agilent Technologies) as previously described (56) ...
-
bioRxiv - Cancer Biology 2021Quote: Whole RNA was extracted from three replicates for each of the cell-lines followed by a quality assessment using Agilent Bioanalyser-2100 with Agilent nano Kit (Agilent Technologies). For each sample ...
-
bioRxiv - Immunology 2022Quote: ... The assay was performed for the next 7 days and each day absorbance was read at 570 nm using Cytation 5 Cell Imaging Multi-Mode Reader (Agilent, BioTek). The assay was performed in triplicate.
-
bioRxiv - Neuroscience 2022Quote: ... the sections or cell containing coverslips were mounted on gelatin coated slides with Fluorescent Mounting Medium (S3023, Dako Cytomation, Glostrup, Denmark). The following primary antibodies were used ...
-
bioRxiv - Biochemistry 2022Quote: ... The resulting mixture was digested with DpnI and 5 µL of the mutagenesis reaction was transformed in XL10-Gold® Ultracompetent Cells (Agilent) and selected on amplicillin-containing LB-agar plates ...
-
bioRxiv - Bioengineering 2022Quote: ... Two-channel 54.8 μm z-stack images were taken every 3 hours for 45 hours after co-culture was initiated using a Cytation 5 cell imaging multimode reader (Agilent Technologies). Images were processed with NIH Fiji-ImageJ and cell coverage was measured for each cell type at every time point by calculating the area within the well covered by cells and dividing it by the total well area ...
-
bioRxiv - Bioengineering 2022Quote: Cells were equilibrated 45 min before metabolic characterization in initial volume 150 μL Seahorse XF base medium (103334-100, Agilent Technologies) containing additional (in mmol L−1 ...
-
bioRxiv - Bioengineering 2022Quote: Cells were dissociated from differentiation wells and seeded at 8 × 104 cells well−1 in CM support medium on Matrigel-coated XFe96 monolayer plates (Agilent Technologies). Culture medium was changed the next day to the well’s respective treatment ...
-
bioRxiv - Cancer Biology 2022Quote: ... General mitochondrial function was assessed based on the Seahorse XF Cell Mito Stress Test protocol (Agilent Technologies, Santa Clara, CA, USA). Basal oxygen consumption rate (OCR ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were then washed and 5 × 105 osteoclasts were seeded onto a XF96 plate containing Seahorse XF RPMI medium (Agilent Technologies). The cells were left for 1 h at 37 °C after which the different metabolic drugs were injected (oligomycin 1μM ...
-
bioRxiv - Cell Biology 2022Quote: ... The absorbance was read at 450 nm (reference at 620 nm) using a Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Biotek).
-
bioRxiv - Cell Biology 2022Quote: ... All cell lines tested negative for mycoplasma using the Mycoplasma Plus™ PCR kit (#302008, Agilent Technologies, Santa Clara, CA, USA).
-
bioRxiv - Microbiology 2022Quote: ... hepaticus versus the tdTomato fluorescent protein (72 h transduction) was quantified in epithelial intestinal HT-29 cells using the Human GE 4x44K v2 Microarray Kit (Agilent Technologies) as we previously detailed [13 ...
-
bioRxiv - Molecular Biology 2022Quote: ... as described previously (67) by using the Seahorse XFp Cell Mito Stress Test Kit and XF glycolysis stress test kit (Agilent Technologies). OCR and ECAR were measured under basal conditions and in response to several metabolic drugs provided in the kit ...
-
bioRxiv - Immunology 2023Quote: Real-time analysis of oxygen consumption rates (OCR) were measured in THP-1 cells using XF24/96 Extracellular Flux Analyzer (Seahorse Bioscience). Cells were seeded in Seahorse XF96 cell culture microplate (#101085-004 ...
-
bioRxiv - Neuroscience 2023Quote: ... of VM cells cultured with the PIEZO1 antagonist GsMTx4 and the PIEZO1 agonist Yoda1 was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyser (Agilent®), in order to assess the mitochondrial respiration and glycolysis ...
-
bioRxiv - Systems Biology 2023Quote: The kinetic data in this study are published and were acquired from xCELLigence assays using the xCELLigence Real Time Cell Analysis (RTCA) Single Plate device (Agilent Technologies) (Dankwa et al ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was stored at −80°C and RNA quality was assessed by Bioanalyzer (Agilent 2100 Bioanalyzer, RNA 6000 Nano Kit for cells, RNA 6000 Pico Kit for EVs, Agilent Technologies).
-
bioRxiv - Immunology 2023Quote: ... 1.5 µM FCCP and 1 µM Rotenone/1 µM Antimycin A from Seahorse XF Cell Mito Stress Test (103015-100, Agilent Technologies), and read using Seahorse XFe96 Analyzer (Agilent Technologies) ...
-
bioRxiv - Biophysics 2022Quote: ... Hela CD95KO and Hela WT stable CD95-mEGFP was assessed using the QIFIKIT® for quantification of cell surface antigens by flow cytometry (K007811-8, Agilent Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: The real-time measurements of OCR of H460-FASN-WT/-KO cells were detected on a Seahorse XF24 Extracellular Flux Analyzer (Agilent Technologies). 1×10^4 cells/well were incubated with XF assay medium (pH 7.4 ...
-
bioRxiv - Cancer Biology 2023Quote: Murine glioma cells (similar low passage N1IC-1/2 and p53-1/2) were seeded in PLL coated XFe24 cell culture microplates (Agilent TEchnologies) at 1.8ᵡ104 cells per well in 250μL BFP (n=10 technical replicates for the baseline experiment and n=5 technical replicates for the cysteine/methionine deprivation experiment ...
-
bioRxiv - Genomics 2023Quote: Cells were allowed to grow to confluency and UV crosslinked using 254nm UV light in Stratalinker 2400 on ice (Stratagene, USA). UV treated cells were lysed to get different cellular fractions (Cytoplasmic ...
-
bioRxiv - Cancer Biology 2023Quote: ... HMLER CD24low were seeded at a density of 104 cells per well in a specialized 96-well Seahorse XF96 V3 PS microplate (101085-004, Agilent Technologies). 48h after ...
-
bioRxiv - Biochemistry 2023Quote: The concentration of iron in the cell cultures was determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Agilent, 5110). Freeze-dried cell samples were digested in concentrated nitric acid at 80 °C overnight.
-
bioRxiv - Biochemistry 2023Quote: ... was used as a template to generate deletion variants for expression in insect cells using the QuickChange II XL site-directed mutagenesis kit (Agilent Technologies). For bacterial expression of the ΔN790 variant ...