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Citations for Agilent :
1851 - 1879 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 5 samples of myoepithelial cells and 5 samples of s-SHIP GFP+ cap cells were submitted and subjected to quality analysis using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). All Samples had an average RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and extracellular acidification rate (ECAR) were determined using a Seahorse XFe96 analyzer and the Seahorse XF Cell Mito Stress Test (Agilent Technologies, Santa Clara, CA). Cells were seeded into Seahorse XFe96 well plates at a density of 7500 cells/well/80 μl ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Cell Biology 2022Quote: ... T cell culture medium was changed 1 hour before the start of readout into 180μL serum-free assay medium (Agilent Seahorse XF DMEM; 103575-100) supplemented with 4mM L-glutamine ...
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bioRxiv - Immunology 2022Quote: Naive CD3+ T cells were placed on PLL-coated Seahorse Bioanalyzer XFp culture plates (3 × 105 cells/well) with Seahorse XF RPMI Assay Media (RPMI Medium, pH 7.4, 103576-100; Agilent Technologies, Santa Clara, CA, USA), supplemented with 10 mM glucose (103577-100 ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Pre-amplification-free single-cell whole genome sequencing libraries were prepared using a Bravo Automated Liquid Handling Platform (Agilent Technologies, Santa Clara, CA, USA), followed by size-selection and extraction from a 2% E-gel EX (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: Respiration and acidification rates were measured on monolayer culture of undifferentiated hPSCs and cells at S5 using a Seahorse XFe96 extracellular flux analyser (Agilent, Santa Clara, CA, US). Geltrex coated XFe96 assay plates were utilized for seeding of 3×103 undifferentiated hPSCs and 2×105 hPSC-derived cardiac cells in each well ...
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bioRxiv - Molecular Biology 2022Quote: ... Respirometry of isolated mitochondria from brain and SPC tissue and from cell cultures was performed using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience; Agilent, Santa Clara, CA). Mitochondrial oxygen consumption rate (OCR ...
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bioRxiv - Immunology 2022Quote: ... and Extracellular Acidification Rate (ECAR) were measured using Seahorse XF Cell Energy Phenotype kit on Seahorse XFp Flux Analyzer (Agilent Technologies, Santa Clara, CA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). Cells were washed in PBS once and incubated in a hypoxic chamber following the manufacturer’s procedure ...
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bioRxiv - Developmental Biology 2022Quote: Oxygen consumption rate (OCR) of mitofusin mutants expressed using adenovirus in H9c2 cells was measured using a Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience, Billerica, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... 50,000 cells per well were plated after 3 days of induction in a specialized Seahorse analyzer 96-well plate (Agilent, cat. no. 103774-100). 10-12 day old i3Neurons were treated with 5 nM vincristine ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture of early/late apoptosis and some necrotic death was induced by irradiating Jurkat cells and the isolated neutrophils under a UV lamp (254nm, Stratalinker UV 1800, Stratagene Corporation, La Jolla, CA) for 1 and 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... The sequence of bPAC-cMyc-IRES-mCherry was carried in pShuttle-CMV vector for generation of adenovirus (AdbPAC) in 293AD cells with the AdEasy system (Agilent Technologies, Santa Clara, CA) (34) ...
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bioRxiv - Immunology 2023Quote: ... The oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) were measured with an XF Cell Mito Stress Kit (Agilent, Santa Clara, CA, USA) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Primary cells isolated from parotid salivary glands were plated on an Agilent Seahorse XF96 Cell Culture Microplate (Part No. 101085-004, Agilent Technologies, Cedar Creek, TX) at a seeding density of 150,000 cells/well and cultured prior to irradiation at 24 hours or 48 hours before running the Seahorse ATP Rate Assay ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7-ESR1Y537S cells were seeded at a density of 3×104 in corresponding treatment media without phenol red in each well of the XFp Cell Culture miniplates (Seahorse Bioscience Inc., Billerica, MA, USA). The next day ...
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bioRxiv - Molecular Biology 2020Quote: Capture probe sequencing of genomic DNA from MCC cell lines and tissues was performed using the SureSelectXT Target Enrichment System for Illumina Paired-End Multiplexed Sequencing Library (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer’s instructions (protocol version B5) ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting library was inserted into pBEL118N phagemid (Lee et al. 2012) and electroporated to TG1 Electroporation-Competent Cells (Agilent Technologies, Santa Clara, CA, USA). Phages containing the library were rescued using M13KO7 Helper Phage (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... The two types of DNA fragments were inserted into pUAST as a head-to-head dimer and transformed into SURE2 competent cells (200152; Agilent Technologies, Santa Clara, CA, USA). Transgenic flies were generated by standard P-element transformation procedures.
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bioRxiv - Molecular Biology 2022Quote: ... Respirometry of isolated mitochondria from brain and SPC tissue and from cell cultures was performed using the Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience; Agilent, Santa Clara, CA). Mitochondrial oxygen consumption rate (OCR ...
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bioRxiv - Plant Biology 2023Quote: ... The quality of the total RNA extracted from target cells was evaluated using the RNA 6000 Pico kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA integrity was determined based on the RNA Integrity Number (RIN ...
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bioRxiv - Cancer Biology 2023Quote: ... The plates were then incubated at 37°C for 48 hours then analyzed by flow cytometry and ELISA or monitored for target cell death using xCelligence eSight (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cancer Biology 2023Quote: ... The cytolysis of A549 cells was monitored over a period of 5 days using xCELLigence® RTCA eSight device (Agilent Technologies, Inc., Santa Clara, CA) by impedance.
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bioRxiv - Cell Biology 2023Quote: ... Seahorse XF cell Mito stress test was performed to measure ECAR and OCR using the Seahorse Extracellular Flux (XFe96) Analyzer (Seahorse Bioscience Inc. North Billerica, MA). On the day of the assay ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...