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Citations for Agilent :
1651 - 1700 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cells were stored at - 80°C until crosslinking (still frozen) with 400 mJ/cm2 UVC using a Stratalinker 2400 (Stratagene).
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bioRxiv - Cell Biology 2023Quote: Oxygen consumption rate of U2OS WT and G3BP1/2 dKO cells was measured on an extracellular flux analyzer (Agilent Seahorse) using the XF Cell Mito Stress Test Kit following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... After single-cell RNA-seq libraries were prepared and the library quality was confirmed with the TapeStation D1000 Screen Tape (Agilent) and a Qubit 3.0 dsDNA HS Assay Kit (Life Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... Two-channel 85 µm z-stack images were taken using a Cytation 5 V3.14 cell imaging multi-mode reader (Agilent Technologies). A total of 21 z-stacks per well and per experiment were recorded and used for post-processing to calculate the cell phenotypic response in each hydrogel model.
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bioRxiv - Physiology 2024Quote: ... MLO-Y4 cells with Pparγ silenced with siRNA (γY4KD) and their scrambled control (Scrl) were plated 6 hrs before the assay onto Seahorse cell culture microplates (Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: Mitochondrial bioenergetics of MDMs and DCs were measured using the Seahorse XF Cell Mito Stress Test Kit and the XF24 Extracellular Flux Analyzer (Agilent), as previously described (100) ...
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bioRxiv - Molecular Biology 2024Quote: Data analysis for cell culture EVs was done using the Spectrum Mill MS Proteomics Workbench software package v 4.2 beta (Agilent Technologies). All extracted spectra were searched against a UniProt database containing human reference proteome sequences ...
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bioRxiv - Molecular Biology 2024Quote: Primary mouse AT2s were isolated and plated as described above with measurement of mitochondrial respiration performed using the Seahorse XF Cell Mito Stress Test Kit (Agilent) or the Seahorse XF Palmitate Oxidation Stress Test Kit (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-Pu.1 (1:200; 2258, Cell Signalling), hamster anti-CD3 (1:500; MCA2690, AbD Serotec) or rabbit anti-GFAP (1:2400, Z0334, Dako) diluted in the blocking solution overnight at 4°C plus one additional hour at room temperature (RT) ...
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bioRxiv - Cancer Biology 2024Quote: ... data was exported to a Seahorse XF Cell Mito Stress Test Report Generator using the Seahorse Wave Desktop Software (Agilent). Hoechst fluorescent intensity was used to normalize for cell number.
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bioRxiv - Cell Biology 2023Quote: ... medium was removed and cells were washed according to manufacturer’s protocol with Seahorse assay medium consisting of DMEM (103575-100, Agilent, USA) with 10 mM glucose ...
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bioRxiv - Biophysics 2024Quote: ... using a 3 × 3 mm black walls quartz cell at 25 °C on an Agilent Cary Eclipse spectrofluorometer (Agilent Technologies) equipped with a thermostatted cell holder attached to an Agilent PCB 1500 water Peltier system ...
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bioRxiv - Bioengineering 2024Quote: The OCR and ECAR of hMSCs was measured using the Seahorse Cell Mito Stress Test with the extracellular flux Seahorse XFp analyzer (Agilent), in order to assess the mitochondrial respiration and glycolysis ...
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bioRxiv - Biochemistry 2023Quote: ... of C2C12 cells expressing UCP1 under the control of the doxycycline-inducible promoter (TRE3G) were measured with Seahorse XFe24 analyzer (Agilent). Cells were seeded at 4 x 104 cells per well in XFe24 cell culture microplates (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: Cellular oxygen consumption rate (OCR) was measured using a Seahorse XF Cell Mito Stress Test in a XFe24 Bioanalyzer (Agilent). Cells were plated at a concentration of 30,000 (HF2303 ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting vectors were subjected to sequencing to ensure that no mutations were introduced into the WRN sequence and were used for transforming BL21 cells (Stratagene). Expression of GST and GST-fusion proteins were induced upon addition of 1 mM isopropyl-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed in 1X PBS and exposed to UV (10 J/m2) in a Stratalinker UV crosslinker oven (Stratagene).After UV exposure ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed by NovoCyte 3000 flow cytometer equipped with three lasers and 13 fluorescence detectors (Agilent, Santa Clara, CA). GFP and Alexa Flour 647 fluorophores were excited by the 488 and 640 nm lasers ...
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bioRxiv - Cell Biology 2020Quote: Oxygen consumption rate (OCR) was measured during Seahorse XF Cell Mito Stress Tests as per the user guide (Agilent 103015-100) with modifications ...
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bioRxiv - Cell Biology 2020Quote: ... The cell viability was assessed by measuring the absorbance at 570 nm wavelength using Epoch 2 microplate spectrophotometer (Agilent technologies. USA).
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... we washed the cells twice with 1X PBS and incubated them with FITC-conjugated anti-mouse IgG (dilution 1:500; Dako Cytomation) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... The PGBD1 ORF was amplified from a cDNA library prepared from HeLa cells using DNA polymerase Pfu Ultra II Fusion HS (Agilent Technologies) and PGBD1 Forw (5’-ATGTATGAAGCTTTGCCAGGC-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... all cultures were diluted to seed an A600 = 0.01 cells/well into a poly-D-lysine coated Seahorse XFp cell culture miniplate in 50 μl of Seahorse XF assay media (Agilent Technologies). For background measurement ...
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bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
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bioRxiv - Biophysics 2019Quote: ... Endothelial cell phenotype was confirmed by the typical cobblestone appearance and von Willebrand Factor expression (A0082 Dako-Agilent Santa Clara CA) (Supplemental Figure 1) ...
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bioRxiv - Cell Biology 2019Quote: ... and FACS-isolated eYFP positive cells (FACS ARIA IIu sorter, Becton Dickinson) were collected in Lysis Buffer (400753, Absolutely RNA Nanoprep kit, Agilent Technologies).
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bioRxiv - Developmental Biology 2019Quote: ... quality and integrity of RNA samples obtained from HUVEC cell bodies and protrusions were assessed using a 2200 TapeStation (Agilent Technologies). Next ...
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bioRxiv - Neuroscience 2020Quote: Viral particles of AAV2 were packaged in transfected 293T cells with other two plasmids: pAAV-RC and pAAV-Helper (Agilent Genomics). After harvest ...
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bioRxiv - Bioengineering 2021Quote: ... were measured in hypoxic hCMEC/D3 monolayers (20,000 cells/well cultured for four days) in a Seahorse XF96 plate by XF analysis (XF24, Agilent Seahorse Technologies) as previously described [43] ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... or IRBITKO cells was subjected to PCR amplification (Herculase II Fusion DNA Polymerase and 5X Herculase II PCR Buffer and dNTP (Agilent Techologies) using primers flanking the region targeted by the gRNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1.5 μL of the mutagenesis reaction was transformed into SoloPack Gold supercompetent cells following the manufacturer’s protocol (Product Number 230350, Agilent, Wilmington, DE). After a 1 hour recovery ...
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bioRxiv - Microbiology 2021Quote: ... Cell extracts were each resuspended in 50 μl methanol and 10 μl applied to high-performance liquid chromatograph (HPLC) (Agilent Technologies). AzoSM was detected at 365 nm on a C-8 column with a flow rate of 0.5 ml/min in methanol/water 90:10 ratio buffered with 1mM ammonium formate and 0.2% formic acid (Figure S8) ...
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bioRxiv - Microbiology 2021Quote: ... using a Cary WinUV UV-visible spectrophotometer equipped with a Cary Dual Cell Peltier accessory (Agilent Technologies, Santa Clara, CA, USA). Reactions were carried out at 25 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: A pET-24B plasmid containing Marseillevirus marseillevirus doublet histones (Hδ-Hγ with a C-terminal 6×His tag and Hβ-Hα) (Valencia-Sanchez et al., 2021) was transformed into Escherichia coli BL2-codon plus(DE3)-RIL competent cells (Agilent Technologies) and cultured at 37°C in 2xYT-Kanamycin medium ...
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bioRxiv - Cancer Biology 2022Quote: ... Target cells were quantified based on the number of GFP+ objects identified per scanned area using BioTek Gen5 Imager Software (Agilent Technologies). To assess T cell activation based on cytokine release ...
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bioRxiv - Cell Biology 2022Quote: ... Specially designed polystyrene tissue culture-treated 96-well microplates with a clear flat bottom (Seahorse XF96 V3 PS Cell Culture Microplates; Agilent Technologies) were then seeded with 80 μl of cell suspension (4.4×105 cells/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... ECAR (mpH/min) and OCR (pmol/min) values were obtained using Seahorse XFp Cell Energy Phenotype Test Kit (Seahorse Bioscience Inc.). Experiments were performed in triplicate and repeated at least three times.
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bioRxiv - Neuroscience 2022Quote: ... CAS No 1582-09-8) was accessed using the Seahorse XF 96 Extracellular Flux Analyzer and the XF Cell Mito stress test kit (Agilent Technologies) following Agilent Technologies guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were tested for mycoplasma at the beginning of the studies (tested negative with MycoSensor PCR Assay Kit, Agilent, #302109) and have never exhibited contamination symptoms after initial testing.
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bioRxiv - Cancer Biology 2022Quote: ... Plates were read at 450 nm and 560 nm wavelengths using a BioTek Cytation 3 Cell Imaging Multi-Mode Microplate Reader (Agilent Technologies). Plasma samples isolated from retroorbital bleeds were used for ProcartaPlex immunoassays (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: Mitochondrial function was evaluated to assess typical bio-energetic profile of cells by directly measuring Oxygen consumption rate (OCR) in differentiated groups using Seahorse XFP (Seahorse Bioscience, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
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bioRxiv - Cell Biology 2021Quote: ... they were prepared and assayed for mitochondrial function assessment according to manufacturer instructions (Seahorse XF Cell Mito Stress Test Kit, Agilent Technologies). More details are found in Supplemental Methods.
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2020Quote: ... The PmeI-linearized pShuttle-vectors carrying different cDNA fragments were introduced into the replication-deficient adenoviral vector pAdEasy-1 through homologous recombination in BJ5183 cells (Agilent Technologies). Successfully recombined adenoviral vector was verified by sequencing ...
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bioRxiv - Biophysics 2021Quote: HIV-1 Pol constructs of the BH10 strain (gift from Michael Parniak) were cloned into a pET28a vector and transformed into BL21-CodonPlus (DE3)-RIL cells (Agilent Technologies) alone or co-transformed with MBP-IBD ...