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Citations for Agilent :
1501 - 1550 of 1879 citations for Rat Cell Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The cells were incubated with anti-tubulin (Thermo PA1-41331; dilution 1:250) and K9JA (Dako A0024; dilution 1:500) antibody ...
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bioRxiv - Cell Biology 2020Quote: ... fetal and adult CFs were seeded onto dopa-coated Seahorse XF96-well cell culture microplates (V3 PS, Agilent, 101085-004) at a density of 123k/cm2 ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Neuroscience 2020Quote: ... Extracellular acidification rates (ECAR) and oxygen consumption rates (OCR) were measured via the Cell Mito Stress Test (Agilent, 103015-100) with a Seahorse XFe96Analyzer (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... was fused with a six histidine tag (6xHis) and an AviTag (pET-28a-AviTag-GluN1C0) and expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent). Expression of His-AviTag-GluN1(834-863 ...
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bioRxiv - Cell Biology 2022Quote: ... of hPASMC were measured using Seahorse XF96 metabolic analyzer and Seahorse XF Cell Mito Stress Test kit (103015-100, Seahorse XF Technology, Agilent), following manufacturer’s recommendations ...
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bioRxiv - Biophysics 2019Quote: Human CAMSAP1 residues 1474-1613 encompassing the CKK domain (HsCKK) were cloned into pET28a vector and expressed in BL21(DE3) cells (Stratagene). Following purification via immobilized metal-affinity chromatography (IMAC ...
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bioRxiv - Physiology 2019Quote: Oxygen consumption rates in C2C12 cells were measured with a Seahorse Flux Analyzer XF24 or XFe96 (Seahorse Bioscience, Billerica, MA). The cells were plated at ∼20,000-40,000 cells/well and then differentiated into myotubes ...
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bioRxiv - Immunology 2019Quote: ... BMDMs were seeded at 5×104 cells/well and analyzed the following day on a Seahorse XF 96well Analyzer (Agilent).
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bioRxiv - Immunology 2019Quote: ... Epithelial cells in the EDTA fraction were depleted by incubation with anti-human epithelial antigen antibody (clone Ber-EP4, Dako) followed by anti-mouse IgG dynabeads (ThermoFisher ...
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bioRxiv - Developmental Biology 2019Quote: ... were dissociated and 106 cells were used to extract total RNA following manufacturer’s instructions of the Absolutely RNA Miniprep Kit (#400800, Agilent Technologies). cDNA was synthesised with 200 ng-1 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... RNA isolated from these cells was checked for integrity using the Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA) and quantified using a Qubit fluorometric assay (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: The Mitochondrial Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in MDA-MB-231 cells were assessed by Agilent Seahorse XF Cell Mito Stress Test Kit and Agilent Seahorse XF Cell Energy Phenotype Test Kit (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... cells were treated at a final concentration of 10μg/ml Poly(I:C) or sonicated salmon sperm DNA (Agilent, Stockport, UK) for 72hrs ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in quadruplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Hippocampal neurons were seeded in a poly-d-lysine-coated XF 96-well cell culture microplate (Seahorse Bioscience, Copenhagen, Denmark) in triplicate at 5 × 105 cells per well in 1 ml neuronal growth medium and then incubated at 37 °C in 5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... For bacterial expression of mEGFP::HCF in E.coli BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent Technologies, Santa Clara, CA, USA), the cDNA of the fusion protein was cloned in pET21a (Merck KGaA ...
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bioRxiv - Biochemistry 2021Quote: Tpm isoforms Tpm1.6 and Tpm3.1 (with and without Met-Ala-Ser at the N-terminus) were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed three times with PBS and then mounted in Roti® Mount FluorCare Dapi mounting medium (#HP20.1, Dako). Cells were visualised on a Zeiss LSM700 confocal microscope using ZEN software ...
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bioRxiv - Cancer Biology 2021Quote: Multiparameter metabolic analysis of intact cells was performed in the Seahorse XF24 extracellular flux analyzer (Seahorse Bioscience, Billerica, MA, USA) as previously described (12) ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells from BM of non-metastasized breast cancer patients were subjected to immunofluorescent staining for EpCAM (Ber-EP4-FITC, Agilent or HEA-125-PE ...
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bioRxiv - Cancer Biology 2020Quote: ... mononuclear cells were screened accordingly to the method for human DCCs using immunofluorescent staining of the cell suspension with anti-human EpCAM (Ber-EP4-FITC, Agilent, or HEA-125-PE ...
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bioRxiv - Cancer Biology 2020Quote: Simultaneous multiparameter metabolic analysis of live cells was performed in the Seahorse XF24®extracellular flux analyzer (Seahorse Bioscience, USA). ACC-489 and CCL-136 cells were seeded in XF24 V7 multi-well plates (15,000 cells per well ...
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bioRxiv - Neuroscience 2020Quote: AAV vectors used in the study were produced in human embryonic kidney HEK 293 cells (AAV-293, Agilent, RRID: CVCL_6871) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride (CsCl ...
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bioRxiv - Biochemistry 2021Quote: The oxygen consumption rate (OCR) of the MEF cells was measured using the Seahorse XFe24 Extracellular Flux Analyzer (Agilent Technologies). Briefly ...
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bioRxiv - Systems Biology 2021Quote: ... and organic acids from cell cultures were measured by an 1100 Series HPLC system equipped with a 1200 Series refractive index detector (RID) (Agilent) and a Diode array detector (DAD ...
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bioRxiv - Plant Biology 2021Quote: ... pGEX6P1:SYT1C2B (residues G374-end) and pGEX6P1:SYT1-SMPC2A (residues N34-E397) were transformed into Rosetta 2 (DE3) pLysS cells (Stratagene) for protein expression using standard protocols ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or with minor N-terminal truncations to exclude the transmembrane regions (Sphaeroforma arctica: amino acids 21-316; Auxenochlorella protothecoides: amino acids 21-327) in Arctic Express DE competent cells (Agilent). At the N-terminus ...
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bioRxiv - Neuroscience 2020Quote: Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) were measured from the plated astrocytes using the Seahorse XFe96 Cell Metabolism Analyzer (Agilent). Astrocytes were dissociated to single cells and plated at a density of 1×104 per well ...
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bioRxiv - Neuroscience 2020Quote: ... iPS-Mg were plated and matured on Seahorse cell culture microplates and analysed using a Seahorse XFe96 Analyser (Agilent Technologies) as previously described (Piers et al ...
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bioRxiv - Genomics 2021Quote: ... and electroporated in five separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent). After a 1 hr outgrowth in Luria Broth (LB ...
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bioRxiv - Microbiology 2020Quote: ... and extracellular acidification rate (ECAR) were measured on Seahorse XF96 analyzer using Seahorse XF Cell Mito Stress Test Kit (Agilent). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Cell Biology 2020Quote: ... One hour before the analysis the cells were washed twice in phenol red and bicarbonate free DMEM Seahorse Media (SM) (part nb 103575-100, Agilent) containing 25 mM glucose (Cat nb ...
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bioRxiv - Cell Biology 2020Quote: ... One hour before the analysis the cells were washed twice in SM phenol red free and bicarbonate free DMEM (part nb 103335-100, Agilent), 25 mM glucose ...
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bioRxiv - Biophysics 2021Quote: hnRNPA1* (where * denotes that the hexa-peptide 259-264 is deleted) protein and deletion constructs were expressed as N-terminally tagged hSUMO fusion proteins in BL21 (DE3) RIPL cells (Agilent) in LB media ...
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bioRxiv - Biophysics 2021Quote: ... cells were resuspended by 200 μL 1% (w/v) BSA in PBS for detection by a NovoCyte flow cytometry (Agilent).
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bioRxiv - Cancer Biology 2021Quote: ... Animals treated with CAR T cells had tumors excised and were also stained with H&E and human CD3 (A0452, Dako), in addition to GPC2 ...
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bioRxiv - Cell Biology 2021Quote: ... to exclude the dead cell population and directly analyzed using flow cytometry on a Novocyte flow cytometer (Acea Biosciences, Agilent). The population of interest was gated according to its FSC/SSC criteria ...
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bioRxiv - Cell Biology 2020Quote: ... The OCR was then measured with an XF24 Extracellular Flux Analyzer via the XF Cell Mito Stress Kit (Agilent Seahorse). Following 5 measurements of basal OCR ...
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bioRxiv - Immunology 2021Quote: ... 3×105 CD8+ T cells were plated onto poly-D-lysine coated wells and assayed in XF RPMI medium (Agilent) pH 7.4 supplemented with 10 mM glucose and 2 mM glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated in 100 ng/mL DAPI for 10 minutes before mounting slides using the Fluorescent mounting medium (DAKO). Cells were visualized on Zeiss LSM780 confocal microscope using the ZEN software ...
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bioRxiv - Biochemistry 2022Quote: ... These L6 myoblasts were seeded 24 h before experiments at 30,000 cells per well on either XF24 tissue culture plates (Agilent-Seahorse) for extracellular flux analysis ...
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bioRxiv - Biochemistry 2022Quote: Oxygen consumption rate (OCR) and extracellular acidification (ECAR) were measured using the Seahorse XF Cell Energy Phenotype Test Kit (Agilent). 12 × 103 cells per well were reverse transfected with siRNA control or targeting SORD in a Seahorse XF24 Cell Culture Microplate (Agilent ...
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bioRxiv - Immunology 2022Quote: ... approximate ~1 ×105 TH17 cells were suspended in 50 μL assay medium (Seahorse XF RPMI Assay Medium, pH 7.4, Agilent Technologies) containing 10 mM glucose ...
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Pathology 2022Quote: ... Anti-CD138 antibody was used for detection of plasma cells by immunohistochemistry (MI15 clone; Agilent Dako, Santa Clara, California, USA) at 1:25-1:50 dilution range.
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bioRxiv - Genetics 2022Quote: Total RNA of each cell line was extracted 48 h after seeding and quality was assessed on electrophoretic spectra from a Fragment Analyzer (Agilent), analyzed with the PROSize software (v ...
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bioRxiv - Cell Biology 2022Quote: ... RPE-1 cell lines ectopically expressing HyPer2-DAO-NES or HyPer2-DAO-NLS were generated by viral transduction of cells using an rAAV gene targeting system (Stratagene) and subsequent isolation of single Hyper2-fluorescent clones by cell sorting on a BD FACSAria III (BD Biosciences) ...