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Citations for Agilent :
1351 - 1400 of 1403 citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... qRT-PCR reactions were made for selected genes using the Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix (Agilent Technologies) as previously described.
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bioRxiv - Genomics 2019Quote: ... Gene expression was performed on 7900 HT fast real time PCR system (Applied Biosystem, USA) using 2× Brilliant III SYBR@ Green qPCR Master Mix (Agilent Technologies, USA) and gene specific primers (Supplementary Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Finally ...
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bioRxiv - Biochemistry 2022Quote: ... The phPAI-1 library was generated by error prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) with primers that maintain the AscI and NotI restriction sites (SI Table 1) ...
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bioRxiv - Molecular Biology 2022Quote: ... Adapter dilution (1:4) and PCR amplification (26 cycles) were optimized in a pilot experiment using Bioanalyzer DNA1000 (Agilent, Sant Clara, USA) chips as a read out of library size and quantity ...
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bioRxiv - Molecular Biology 2022Quote: ... The purity and integrity of the PCR products were analysed on an Agilent 2100 Bioanalyzer following manufacturer recommendations for high sensitivity DNA chips (Agilent 5067-4626). Sequencing libraries were prepared using Nextera DNA Library Prep Kit (Illumina FC-131-1024 ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified mRNA was transferred to a clean nuclease-free PCR tube before the yield and size distribution was assessed using an RNA Pico Chip (Agilent Technologies, Inc.).
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bioRxiv - Molecular Biology 2024Quote: ... Amplification and melting curve analysis (see supplementary Figure S1) and monitoring were performed using Agilent AreaMx Real-Time PCR System (Agilent Technologies, USA).
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bioRxiv - Plant Biology 2024Quote: Quantitative PCR (qPCR) was carried out in a 25 μL reaction volume using the Brilliant II SYBR Green PCR Master Mix (Stratagene, Agilent technologies). Primer concentrations were set at 0.2 μM ...
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bioRxiv - Physiology 2023Quote: ... Mutations were introduced by PCR-based site-directed mutagenesis method using the Quick Change II XL Site-Directed Mutagenesis Kit (Agilent Technologies, USA) and verified by sequencing (Supplementary Table 2).
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bioRxiv - Immunology 2023Quote: ... The analysis of the PCR products was performed using the LabChip electrophoretic Agilent 2100 Bioanalyzer microchip electrophoresis system (Agilent Technologies, Wokingham, UK). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... the A3 sequence specifying amino acids 21-393 was amplified from pHD1344tub(PAC)-Nterm3V5-A3 by error prone PCR using the GeneMorphII Random Mutagenesis kit (Agilent, Cat # 200523) and primers that add AttB sites (Table S2 ...
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bioRxiv - Cancer Biology 2023Quote: Mitochondrial DNA copy number was quantified by qPCR on total DNA from gastrocnemius muscle using MxPro Mx3000P real-time PCR Analyzer (Agilent technologies / Stratagene) and determining the mtDNA/nDNA ratio ...
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bioRxiv - Biochemistry 2023Quote: ... All UTX isoforms and mutants were generated by PCR cloning into the pDONR221 vector or by site-directed mutagenesis using the QuikChange strategy (Agilent, CA, USA). Inserts were transferred to the pCDNA5_FRT_TO_N-GFP destination vector using GATEWAY cloning according to instructions of the manufacturer (Life technologies ...
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bioRxiv - Systems Biology 2023Quote: ... 7 cycles were used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System) and DNA 7500 (Agilent 2100 Bioanalyzer) ...
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bioRxiv - Systems Biology 2023Quote: The genomic integrated sgRNA libraries were amplified and indexed in two rounds of PCR using the Herculase II Fusion DNA Polymerase kit (Agilent Technologies 600679). The libraries were sequenced on an Illumina NextSeq with a custom primer oMCB1672 for read 1 and ≥21 cycles in read 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... The CrxE1/CAG::OC1 element was cloned using polymerase chain reaction (PCR) with the high fidelity Herculase II Fusion DNA Polymerase (Agilent cat# 600675) to add “Sal1” sites to the 261bp enhancer sequence ...
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bioRxiv - Microbiology 2024Quote: ... 80 µL of the supernatant was removed before exposing the plate to a temperature gradient for 3 minutes in a PCR machine (Agilent SureCycler 8800), followed by 3 minutes at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Complementation of ΔfimB mutants were performed by cloning the promoter and encoding regions of the parental fimB genes into pSC-A-amp/kan (Table S4) by using the StrataClone PCR Cloning Kit (Agilent Technologies, Massy, France). The recombinant plasmid was then electroporated into competent strains S250ΔfimB: ...
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bioRxiv - Molecular Biology 2021Quote: ... IMAGE:6292760)) cloned in the pOTB7 vector (IRAUp969D10104D, RZPD Imagenes, Germany) served as template for site-directed PCR-mutagenesis (QuickChange, Stratagene, La Jolla, CA) according to the manufacturer’s instructions and as described in [52] and using the primer pairs (10 nmol ...
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bioRxiv - Physiology 2019Quote: ... amplified products were concentrated using a solid-phase reversible immobilization method for the purification of PCR products and quantified by electrophoresis using an (Agilent, Santa Clara, CA) 2100 Bioanalyzer ...
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bioRxiv - Microbiology 2019Quote: ... Site-directed mutagenesis of the spaO gene was performed according to the QuikChange PCR site-directed mutagenesis protocol (Agilent, Santa Clara, CA, USA). All primers used in this study can be found in Table S5.
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bioRxiv - Cell Biology 2020Quote: pSecTagNC + ΔEGF_mN1 W1758A and pSecTagNC + ΔEGF_mN1 R1994A: these plasmids were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pSecTagNC + ΔEGF_mN1 as a template and primers ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD R1994A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primer A2026VmutF.
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bioRxiv - Plant Biology 2019Quote: ... DNA fragments carrying both adapters after sodium bisulfite conversion were enriched by 8 cycles of PCR using the Pfu Turbo Cx Hotstart DNA Polymerase (Agilent, Santa Clara, CA). Primer dimers were subsequently removed using two rounds of purification using AMPure beads (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... The anc-1 3’UTR RNAi construct was generated by PCR amplifying part of the 3’UTR-region of the anc-1 transcript using Paq5000™ DNA Polymerase (Agilent Technologies, 600682). The primer sequences are detailed in Table S2 ...
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bioRxiv - Neuroscience 2020Quote: ... Fragment size was determined by loading 1 ng/μl cleaned up PCR product on the Fragment Analyzer System (Agilent Technologies, Santa Clara, USA) using the qualitative DNA Kit dsDNA Reagent 35-5000bp (Cat No ...
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bioRxiv - Microbiology 2020Quote: ... Full-length Vero-PVR1 and Vero-PVR2 cDNA was amplified with the Easy-A High Fidelity PCR Cloning Enzyme (Agilent Technologies, CA, USA), and cloned into T-vector pMD20 (Takara Bio Inc.) ...
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bioRxiv - Cell Biology 2021Quote: Both PCRs were performed in 100 µl with the 2 µl of the Herculase II Fusion DNA Polymerase from Agilent (ref. no. 600675) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Plant Biology 2021Quote: ... The genomic SPL7 (AT5G18830.1) coding region (translational start to stop codon) was PCR-amplified and cloned into the pBluescript SK+ vector (Stratagene/Agilent Technologies, Waldbronn, Germany) which was used a PCR template for site-directed mutagenesis (A279T ...
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bioRxiv - Cancer Biology 2022Quote: ... The locus-specific HDR donors were generated by PCR amplification of the MACHETE bicistronic cassette using a high-fidelity DNA polymerase (Herculase II, Agilent or Q5, NEB). PCR fragments were column purified (Qiagen ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... and retrotranscribed as previously described.16 cDNAs were quantified by quantitative real-time PCR on a MX3000 apparatus (Stratagene, La Jolla, CA, USA) by using specific primers ...
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bioRxiv - Systems Biology 2023Quote: ... A total of 13 cycles was then used for the Sample Index PCR reaction and final libraries were evaluated using D5000 ScreenTape (Agilent 4200 TapeStation System). Each type of library was pooled and sequenced using Illumina NovaSeq6000 System on SP flowcells [61] ...
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bioRxiv - Genomics 2023Quote: ... The PCR products obtained were purified (AMPure XP system) and library quality was assessed on an Agilent Bioanalyzer 2100 system (Agilent Technologies, United States). Library preparations of RNA were sequenced on the Illumina NovaSeq 6000 platform (Illumina Inc ...
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bioRxiv - Biochemistry 2023Quote: ... was performed in duplicate using TaqManTM Fast Advanced Master Mix (Cat # 44-445-57) for mRNA in a Mx3000p Real-Time PCR System (Stratagene, Santa Clara, CA). The primers for the qPCR were purchased from Thermo Fisher Scientific/TaqMan ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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bioRxiv - Microbiology 2024Quote: ... Quality assessment and quantification of the PCR products were respectively performed using the Agilent 2100 Bioanalyzer system (Agilent Technologies, Palo Alto, CA, USA) and a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... analysis was performed using TransStart Tip Green qPCR SuperMix kit (TransGen Co.) on a Stratagene Mx3000P real-time PCR system (Stratagene, La Jolla, CA, USA) under the conditions of 94 °C for 30 s ...
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bioRxiv - Molecular Biology 2022Quote: ... All genes were analyzed with the SYBR green detection method (PCR Biosystems, UK) using the Stratagene Mx3000P real[time QPCR system (Agilent Technologies, Santa Clara, USA). Gene expression data were normalized against housekeeping genes (Gapdh in primary osteoblasts and Atp5b in bone tissue ...
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bioRxiv - Immunology 2022Quote: ... PCR amplification was performed on diluted lysis product (1:20 dilution) and 25 µl of PCR reaction mix according to the manufacturer instruction (Herculase II Fusion DNA Polymerase, Agilent Technologies, Les Ulis, France) using the following PCR primers ...
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bioRxiv - Developmental Biology 2019Quote: ... quantitative real time PCR was performed (PerfeCTa® SYBR® Green SuperMix for iQ™, Quanta Biosciences or Brilliant III Ultra Fast QPCR Master Mix, Agilent Technologies). Each sample was analysed in 3 biological replicates and each biological replicate was analysed in technical triplicates using primer pairs in Table S5.
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bioRxiv - Evolutionary Biology 2021Quote: Mutants in the VPg cistron were synthesized by long inverse site-directed PCR mutagenesis using the QuickChange II XL Kit (Stratagene, San Diego CA, USA) following the manufacturer’s instructions and using p35STunos as template ...
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bioRxiv - Biochemistry 2021Quote: ... were obtained by performing a directed evolution starting from the library described above and a random mutagenesis library constructed by error-prone PCR (Agilent, Santa Clara, CA, USA). We used a FRAD to perform directed evolution under identical conditions to our previous study36 ...
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bioRxiv - Immunology 2021Quote: ... The size of the amplicon was confirmed by analyzing 2 μl of PCR products using the Agilent D5000 ScreenTape System (Agilent D5000 ScreenTape, 5067-5588, Agilent D5000, Reagents 5067-5589). Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... The cDNA of Affilin variant Af2p was used as template for error-prone PCR employing the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA, USA). The error rate was set to 10-14 mutations per kbp and the resulting theoretical library size was calculated to contain 6·1010 independent variants ...
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bioRxiv - Biochemistry 2023Quote: ... A mutant Acr library was made using ACX175 as a backbone and error-prone PCR with low mutation rate using GeneMorph II random mutagenesis kit (Agilent, Santa Clara, CA, USA). The resulting library was subcloned into a second plasmid containing AiEvo2 ...
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bioRxiv - Physiology 2023Quote: ... Standard curves for each gene were generated by cloning qPCR products into the pSCA vector with the Strataclone PCR cloning kit (Agilent, Santa Clara, CA, USA), isolating plasmid DNA using the GeneJET Plasmid Miniprep Kit (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Point mutation of D5R-STOP from D5-TANGO was made by polymerase chain reaction (PCR) using the QuikChange II Site–Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The 5D3-scFv-CP33 gene string and the opposite variant were amplified by PfuUltra II Hotstart PCR Master Mix (Agilent, Santa Clara, CA, USA) using 0.8 µM primers specified in S1 Table ...